Urine miR-21-5p and miR-200c-3p as potential non-invasive biomarkers in patients with prostate cancer.

To evaluate miR-21-5p and miR-200C-3p expression in the urine of patients with prostate cancer (PCa) and to investigate their potential as biomarkers.The urine samples collected from 80 patients, including 20 patients who were diagnosed with benign prostatic hyperplasia ( BPH) and 60 patients diagnosed with PCa, were examined. The exosome isolation is done by using miRCURY exosome isolation kit (Exiqon, Denmark), total RNA was extracted using the miRCURY RNA Isolation Kit-Biofluid kit (Exiqon, Denmark), and complementary DNA (cDNA) was synthesized using a kit Universal cDNA Synthesis (Exiqon, Denmark ).

A quantitative polymerase chain reaction (qPCR) analysis of gene expression was performed using qPCR CFX 96 thermocycler (Bio-Rad). All procedures followed by manufacturers recommendations.The overexpressions of miR-21 in a group of non-metastatic PCa and metastatic PCa compared with BPH group was statistically significant with p-values ​​of 0.001 and 0.018, respectively. The non-metastatic PCa compared with metastatic PCa group was also statistically significant with a p-value of 0.037. Under the expression of miR-200C in non-metastatic PCa and PCa metastasis group compared to the BPH group was statistically significant with p-values ​​of 0.001 and 0.001, respectively.

The overexpressions of miR-21 was found in this study could be a potential non-invasive diagnostic tool for patients with PCa. Despite the significant results in our study, the use of micro-RNA in urine samples may vary due to epigenetic variation. Further studies with a larger population is needed to investigate the role of miR-21 and miR-200C as biomarkers in PCa.

 Urine miR-21-5p and miR-200c-3p as potential non-invasive biomarkers in patients with prostate cancer.
Urine miR-21-5p and miR-200c-3p as potential non-invasive biomarkers in patients with prostate cancer.

Circulating microRNAs in extracellular vesicles as a potential biomarker for psoriatic arthritis in patients with psoriasis.

Psoriatic arthritis (PsA) develops in ~ 30% of patients with psoriasis. The diagnosis of PsA challenging and there is no reliable molecular markers in clinical use. MicroRNAs are short noncoding regulatory RNAs, which can be actively packaged in extracellular vesicles (EV) and secreted into circulation.To investigate whether plasma-derived microRNAs EV can serve as a biomarker for PsA in patients with psoriasis.


METHOD
plasma samples obtained from patients with psoriasis the skin only (PSC) and patients with psoriasis and PsA. EVS plasma was isolated using Exosome miRCURYTM Isolation Kit. RNA sequencing was used to identify differentially expressed miRNAs EV in discovery phase (PSC, n = 15; PsA, n = 14). In the validation phase (PSC, n = 29; PsA, n = 28), 41 miRNAs been analyzed in plasma EVs by qPCR. Association of miRNAs identified with PsA assessed by logistic regression analysis.


RNA sequencing identified 19 miRNAs EV plasma levels differed significantly between PsA and PSC in the category of inventions. significantly lower levels of let-7b plasma EV-5p and miR-30e-5p in PsA vs. PSC confirmed in the validation cohort, and decreased their levels found to be associated with the presence of PsA. ROC analysis revealed AUC of 0.68 (95% CI 0.53 to 0.83) for the let-7b-5p and 0.69 (95% CI from 0.55 to 0.84) to the level of miR-30e-5p.Circulating EV microRNA altered in patients with PsA compared with the PSC. The findings of this exploratory study shows that circulating microRNAs EV can serve as a biomarker for arthritis in patients with psoriasis.


The main objective of this research is to develop nano-drug consisting of doxorubicin and exosome derived from mesenchymal stem cells, and to explore its effect on osteosarcoma in vitro.The exosomes isolated from bone marrow MSCs (BM-MSC) by Exosome Isolation Kit.

Exosome precipitation from Plasma + Thrombin
EPStep-T 5 ml
EUR 336.00
ExoQuick Exosome RNA Column Purification kit
EQ808A-1 20 preps
EUR 402.00
Exosome precipitation from Plasma and Serum
EPStep-PS 5 ml
EUR 297.00
ExoQuick-LP for lipoprotein pre-clear & exosome isolation
EXOLP5A-1 5 reactions
EUR 533.00
  • Category: Exosomes
ExoQuick PLUS Exosome Purification Kit - for serum and plasma
EQPL10A-1 10 reactions
EUR 579.00
  • Category: Exosomes
ExoQuick-CG cGMP-grade Exosome Isolation reagent (50 ml)
EXOCG50A-1 50 ml
EUR 1181.00
  • Category: Exosomes
PEG-it Virus Precipitation Solution (100 mL aliquot)
LV810A-1 100 ml
EUR 382.00
  • Category: Lentiviral Technology
PEG-it Virus Precipitation Solution (250 mL aliquot)
LV825A-1 250 ml
EUR 777.00
  • Category: Lentiviral Technology
ExoQuick-TC PLUS Exosome Purification Kit - for tissue culture media
EQPL10TC-1 10 reactions
EUR 579.00
  • Category: Exosomes
ExoQuick Exosome Isolation and RNA Purification kit (for Serum and Plasma)
EQ806A-1 20 preps
EUR 554.00
ExoQuick Exosome Isolation and RNA Purification kit (for Tissue Culture Media)
EQ806TC-1 10 preps
EUR 554.00
SeraMir Exosome RNA Purification kit (5ml ExoQuick and 20 exoRNA columns)
RA806A-1 20 preps
EUR 554.00
  • Category: MicroRNA Tools
Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL)
TMEXO-1 100 reactions
EUR 583.00
  • Category: Exosomes
Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome)
P101EZ NULL
EUR NULL
ExoQuick TC 10ml
EXOTC10A-1 10 ml
EUR 399.00
  • Category: Exosomes
ExoQuick TC 50ml
EXOTC50A-1 50 ml
EUR 1222.00
  • Category: Exosomes
Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome)
P100EZ NULL
EUR NULL
Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome) sample
P101EZ--sample NULL
EUR NULL
DNA Precipitation Buffer
D106 55 ml
EUR 175.00
  • Product category: DNA and RNA extraction and purification
Description: DNA Precipitation Buffer by Cygnus Technologies is available in Europe via Gentaur.
TMB for Precipitation
42R-TB100x 1000 ml
EUR 538.00
Description: High sensitivity TMB Substrate for Precipitation assays
Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome) sample
P100EZ-sample NULL
EUR NULL
SeraMir Exosome RNA Purification kit (10ml ExoQuick-TC and 10 exoRNA columns) 10 preps
RA806TC-1 10 preps
EUR 554.00
  • Category: MicroRNA Tools
Chicken IgY precipitation reagent
85R-1010 4 ml
EUR 554.00
Description: Chicken IgY precipitation reagent for use in immunoassays
50ML Protein Precipitation Kit
NAT1254 50ML
EUR 225.00
PEG Virus Precipitation Kit
K2190-200 200 preparations
EUR 1059.00
PEG Virus Precipitation Kit
K2190-50 50 preparations
EUR 405.00
PEG Virus Precipitation Kit
K904-200
EUR 1001.00
PEG Virus Precipitation Kit
K904-50
EUR 376.00
SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns)
RA800TC-1 10 reactions
EUR 659.00
  • Category: MicroRNA Tools
101Bio High Efficiency Protein Precipitation kit
P5W6 NULL
EUR NULL
In Vitro SDS-K+ Precipitation Kit
TG1010-1 100 assays
EUR 459.00
In Vitro SDS-K+ Precipitation Kit
TG1010-2 250 assays
EUR 571.00
In Vivo SDS-K+ Precipitation Kit
TG1011-1 100 assays
EUR 302.00
In Vivo SDS-K+ Precipitation Kit
TG1011-2 250 assays
EUR 414.00
Psoriasis Exosome
P141-PS NULL
EUR NULL
Lyophilized Exosome Standards
ExoA375 100 μg
EUR 303.50
Lyophilized Exosome Standards
ExoHT29 100 μg
EUR 303.50
Lyophilized Exosome Standards
ExoMCF7 100 μg
EUR 303.50
Lyophilized Exosome Standards
ExoPC3 100 μg
EUR 303.50
Lyophilized Exosome Standards
ExoPLASMA 100 μg
EUR 303.50
Lyophilized Exosome Standards
ExoUrine 100 μg
EUR 303.50
Leukemia - ALL Exosome
P141-ALL NULL
EUR NULL
Leukemia - AML Exosome
P141-AML NULL
EUR NULL
Bladder Cancer Exosome
P141-BL NULL
EUR NULL
Breast Cancer Exosome
P141-BR NULL
EUR NULL
Leukemia - CLL Exosome
P141-CLL NULL
EUR NULL
Leukemia - CML Exosome
P141-CML NULL
EUR NULL
Colorectal Cancer Exosome
P141-CO NULL
EUR NULL
Crohn's Disease Exosome
P141-CR NULL
EUR NULL
Kidney Cancer Exosome
P141-KN NULL
EUR NULL
Lung Cancer Exosome
P141-LG NULL
EUR NULL
Liver Cancer Exosome
P141-LV NULL
EUR NULL
Nasopharyngeal Cancer Exosome
P141-NA NULL
EUR NULL
Prostate Cancer Exosome
P141-PR NULL
EUR NULL
Rheumatoid Arthritis Exosome
P141-RA NULL
EUR NULL
Thyroid Cancer Exosome
P141-TH NULL
EUR NULL
Psoriasis Exosome RNA
P241-PS NULL
EUR NULL
Sodium Pyruvate Solution, 100 mM Solution
CCM1753-100 100 mL
EUR 60.84
  • Product category: Culture Media
TRIMMING SOLUTION
99-676-CM 1 L /pk
EUR 88.00
Description: Specialty Media; Islet products
Denaturing solution
DE540 1L
EUR 61.31
  • Product category: Electrophoresis Related/Reagents
SDS Solution
2102-100
EUR 137.00
EDTA Solution
2103-100
EUR 137.00
AlphaBioCoat Solution
AC001 20 ml
EUR 271.00
Borax Solution
BOR015 15 ml
EUR 56.00
Borax Solution
BOR125 125 ml
EUR 79.00
Borax Solution
BOR500 500 ml
EUR 119.00
Borax Solution
BOR999 1000 ml
EUR 183.00
Orcein Solution
OAA125 125 ml
EUR 66.00
Orcein Solution
OAA500 500 ml
EUR 109.00
Orcein Solution
OAA999 1000 ml
EUR 148.00
Stop Solution
F006 12 ml
EUR 180.00
  • Product category: Buffer solution
Description: Stop Solution by Cygnus Technologies is available in Europe via Gentaur.
Methenamine Solution
MET125 125 ml
EUR 64.00
Methenamine Solution
MET500 500 ml
EUR 95.00
Methenamine Solution
MET999 1000 ml
EUR 132.00
Prehybridization Solution
K2191050-1 5 ml
EUR 137.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Hybridization Solution
K2191050-2 5 ml
EUR 157.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Blocking Solution
K2191050-8 250 ul
EUR 137.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Schiff's Solution
SRF030 30 ml
EUR 64.00
Schiff's Solution
SRF250 250 ml
EUR 77.00
Schiff's Solution
SRF3800 1 Gal.
EUR 345.00
Schiff's Solution
SRF500 500 ml
EUR 103.00
Schiff's Solution
SRF999 1000 ml
EUR 149.00
DTT Solution
R-1104 1 ml, 500 mM
EUR 109.45
Description: fast delivery possible
Tartrazine Solution
TZQ125 125 ml
EUR 64.00
Tartrazine Solution
TZQ500 500 ml
EUR 98.00
Tartrazine Solution
TZQ999 1000 ml
EUR 127.00
Exosome Isolation kit (for serum & plasma -Exosome-RNA-NGS)
P101NGS NULL
EUR NULL
Gentamicin Solution, Gentamicin Solution, 50 mg/mL
CCM1123-010 10 mL
EUR 64.05
  • Product category: Culture Media
ExoQuick ULTRA EV Isolation Kit for Serum and Plasma
EQULTRA-20A-1 20 reactions
EUR 579.00
Exosome Component 5 Protein
20-abx260873
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 25 ug
  • 5 ug
  • Shipped within 5-10 working days.
Exosome Component 7 Protein
20-abx261347
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.
Exosome Component 10 Protein
20-abx261805
  • EUR 4365.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.
Exosome Component 4 Protein
20-abx261954
  • EUR 4490.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.
Exosome Component 9 Protein
20-abx262123
  • EUR 328.00
  • EUR 5089.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.
Exosome Component 8 Protein
20-abx262850
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.
Exosome Component 3 Protein
20-abx263422
  • EUR 230.00
  • EUR 1609.00
  • EUR 328.00
  • 1 µg
  • 50 ug
  • 5 ug
  • Shipped within 5-10 working days.
Exosome Component 1 Protein
20-abx263423
  • EUR 1609.00
  • EUR 328.00
  • EUR 230.00
  • 100 ug
  • 10 ug
  • 2 µg
  • Shipped within 5-10 working days.
FluoroCet Exosome Quantitation Kit
FCET96A-1 96 reactions
EUR 726.00
  • Category: Exosomes
Fluorescent Exosome Standard (Urine )
ESF-15 100 µg
EUR 1723.00
Description: Fluorescent exosomes are labeled with green dye, which offer a stable fluorescent labeling.
EXOCET Exosome Quantitation Kit
EXOCET96A-1 96 reactions
EUR 521.00
  • Category: Exosomes
Exosome-TEM-easy Kit
P130 NULL
EUR NULL
Diabetes (Type 1) Exosome
P141-DB1 NULL
EUR NULL
Diabetes (Type 2) Exosome
P141-DB2 NULL
EUR NULL
Leukemia - Hairy Cell Exosome
P141-HC NULL
EUR NULL
Leukemia - Multiple Myeloma Exosome
P141-MM NULL
EUR NULL
Melanoma (skin cancer) Exosome
P141-MN NULL
EUR NULL
Non-Hodgkin lymphoma Exosome
P141-NH NULL
EUR NULL
Systemic Lupus Erythematosus Exosome
P141-SL NULL
EUR NULL
Uterine Cervical Cancer Exosome
P141-UC NULL
EUR NULL
Uterine Corpus Cancer Exosome
P141-UT NULL
EUR NULL
Normal human exosome RNA
P240 NULL
EUR NULL
Leukemia - ALL Exosome RNA
P241-ALL NULL
EUR NULL
Leukemia - AML Exosome RNA
P241-AML NULL
EUR NULL
Bladder Cancer Exosome RNA
P241-BL NULL
EUR NULL
Breast Cancer Exosome RNA
P241-BR NULL
EUR NULL
Leukemia - CLL Exosome RNA
P241-CLL NULL
EUR NULL
Leukemia - CML Exosome RNA
P241-CML NULL
EUR NULL
Colorectal Cancer Exosome RNA
P241-CO NULL
EUR NULL
Crohn's Disease Exosome RNA
P241-CR NULL
EUR NULL
Kidney Cancer Exosome RNA
P241-KN NULL
EUR NULL
Lung Cancer Exosome RNA
P241-LG NULL
EUR NULL
Liver Cancer Exosome RNA
P241-LV NULL
EUR NULL
Nasopharyngeal Cancer Exosome RNA
P241-NA NULL
EUR NULL
Prostate Cancer Exosome RNA
P241-PR NULL
EUR NULL
Rheumatoid Arthritis Exosome RNA
P241-RA NULL
EUR NULL
Thyroid Cancer Exosome RNA
P241-TH NULL
EUR NULL
Hematoxylin Counterstain Solution
AR0005 12mL(for 120-180 assays)
EUR 65.00
Ponceau S solution
AR0142 12mL(10X)
EUR 60.00
Astra Blue Solution
AST125 125 ml
EUR 104.00
Astra Blue Solution
AST500 500 ml
EUR 183.00
Tyloxapol (10% ) solution
B1159-250
EUR 512.00
Tyloxapol (10% ) solution
B1159-50
EUR 175.00
Acrylamide 40% Solution
A0416-050 495ml
EUR 165.00
Stop/Elution Solution
85R-123 250 ml
EUR 155.00
Description: Elution or stop reagent for use in immunoassays
STOCK POLYSUCROSE SOLUTION
99-662-CVS 300 mL/pk
EUR 188.00
Description: Specialty Media; Islet products
GRADIENT STOCK SOLUTION
99-674-CM 1 L /pk
EUR 614.00
Description: Specialty Media; Islet products
PHASE I SOLUTION
99-678-CM 1 L /pk
EUR 105.00
Description: Specialty Media; Islet products
PENTASTARCH, 10& SOLUTION
99-723-CM 1 L /pk
EUR 386.00
Description: Specialty Media; Islet products
Aniline Blue Solution
ABP125 125 ml
EUR 72.00
Aniline Blue Solution
ABP500 500 ml
EUR 106.00
2% Bisacrylamide Solution
A0114 500ml
EUR 65.66
  • Product category: Electrophoresis Related/Acry/Bis-Acrylamide/Bis-Acrylamide
Acrylamide Solution 30%
A067-1L 1 L
EUR 103.00
Acrylamide Solution 30%
A067-500Ml 500 ml
EUR 71.00
PROTEINASE K SOLUTION
501-PK 5 ml
EUR 152.00
Description: Genomic PCR Grade Proteinase K Solution
Glyoxal, 40% solution
D0238 1Kg
EUR 75.91
  • Product category: Biochemicals/Biology Reagents/Other
Crystal Violet Solution
CVA500 500 ml
EUR 106.00
Crystal Violet Solution
CVA999 1000 ml
EUR 143.00
DAB Differentiating Solution
DDS500 500 ml
EUR 109.00
Congo Red Solution
CRA030 30 ml
EUR 74.00
Congo Red Solution
CRA500 500 ml
EUR 106.00
Congo Red Solution
CRA999 1000 ml
EUR 143.00
Formaldehyde 37% Solution
C5300-1 1L
EUR 72.19
  • Product category: Biochemicals/Biology Reagents/Protein Related
Ringer Solution Tablets
09-1025-100 100 tablets
EUR 70.00
HYGROMYCIN B SOLUTION
30-240-CR 20 mL/pk
EUR 157.00
Description: Media Catalog; Cell Culture Reagents
Ponceau S Solution
22001 1L
EUR 125.00
Description: Minimum order quantity: 1 unit of 1L
HEPES SOLUTION (1M)
25-060-CI 100 mL/pk
EUR 104.00
Description: Media Catalog; Cell Culture Reagents
CometAssay Lysis Solution
4250-010-01 100 mL
EUR 157.80

The exosome-loaded doxorubicin (Exo-Dox) is made by mixing exosome with Dox-HCl, desalinizing with triethylamine and then dialyzing against PBS overnight. Nanoparticle tracking analysis (NTA) and transmission electron microscopy (TEM) was used to characterize of exosome and Exo-Dox.