Urine miR-21-5p and miR-200c-3p as potential non-invasive biomarkers in patients with prostate cancer.

To evaluate miR-21-5p and miR-200C-3p expression in the urine of patients with prostate cancer (PCa) and to investigate their potential as biomarkers.The urine samples collected from 80 patients, including 20 patients who were diagnosed with benign prostatic hyperplasia ( BPH) and 60 patients diagnosed with PCa, were examined. The exosome isolation is done by using miRCURY exosome isolation kit (Exiqon, Denmark), total RNA was extracted using the miRCURY RNA Isolation Kit-Biofluid kit (Exiqon, Denmark), and complementary DNA (cDNA) was synthesized using a kit Universal cDNA Synthesis (Exiqon, Denmark ).

A quantitative polymerase chain reaction (qPCR) analysis of gene expression was performed using qPCR CFX 96 thermocycler (Bio-Rad). All procedures followed by manufacturers recommendations.The overexpressions of miR-21 in a group of non-metastatic PCa and metastatic PCa compared with BPH group was statistically significant with p-values ​​of 0.001 and 0.018, respectively. The non-metastatic PCa compared with metastatic PCa group was also statistically significant with a p-value of 0.037. Under the expression of miR-200C in non-metastatic PCa and PCa metastasis group compared to the BPH group was statistically significant with p-values ​​of 0.001 and 0.001, respectively.

The overexpressions of miR-21 was found in this study could be a potential non-invasive diagnostic tool for patients with PCa. Despite the significant results in our study, the use of micro-RNA in urine samples may vary due to epigenetic variation. Further studies with a larger population is needed to investigate the role of miR-21 and miR-200C as biomarkers in PCa.

 Urine miR-21-5p and miR-200c-3p as potential non-invasive biomarkers in patients with prostate cancer.
Urine miR-21-5p and miR-200c-3p as potential non-invasive biomarkers in patients with prostate cancer.

Circulating microRNAs in extracellular vesicles as a potential biomarker for psoriatic arthritis in patients with psoriasis.

Psoriatic arthritis (PsA) develops in ~ 30% of patients with psoriasis. The diagnosis of PsA challenging and there is no reliable molecular markers in clinical use. MicroRNAs are short noncoding regulatory RNAs, which can be actively packaged in extracellular vesicles (EV) and secreted into circulation.To investigate whether plasma-derived microRNAs EV can serve as a biomarker for PsA in patients with psoriasis.


METHOD
plasma samples obtained from patients with psoriasis the skin only (PSC) and patients with psoriasis and PsA. EVS plasma was isolated using Exosome miRCURYTM Isolation Kit. RNA sequencing was used to identify differentially expressed miRNAs EV in discovery phase (PSC, n = 15; PsA, n = 14). In the validation phase (PSC, n = 29; PsA, n = 28), 41 miRNAs been analyzed in plasma EVs by qPCR. Association of miRNAs identified with PsA assessed by logistic regression analysis.


RNA sequencing identified 19 miRNAs EV plasma levels differed significantly between PsA and PSC in the category of inventions. significantly lower levels of let-7b plasma EV-5p and miR-30e-5p in PsA vs. PSC confirmed in the validation cohort, and decreased their levels found to be associated with the presence of PsA. ROC analysis revealed AUC of 0.68 (95% CI 0.53 to 0.83) for the let-7b-5p and 0.69 (95% CI from 0.55 to 0.84) to the level of miR-30e-5p.Circulating EV microRNA altered in patients with PsA compared with the PSC. The findings of this exploratory study shows that circulating microRNAs EV can serve as a biomarker for arthritis in patients with psoriasis.


The main objective of this research is to develop nano-drug consisting of doxorubicin and exosome derived from mesenchymal stem cells, and to explore its effect on osteosarcoma in vitro.The exosomes isolated from bone marrow MSCs (BM-MSC) by Exosome Isolation Kit.

ExoQuick PLUS Exosome Purification Kit - for serum and plasma

EQPL10A-1 10 reactions
EUR 694.8

ExoQuick-CG cGMP-grade Exosome Isolation reagent (50 ml)

EXOCG50A-1 50 ml
EUR 1417.2

PEG-it Virus Precipitation Solution (100 mL aliquot)

LV810A-1 100 ml
EUR 458.4

PEG-it Virus Precipitation Solution (250 mL aliquot)

LV825A-1 250 ml
EUR 932.4

ExoQuick-TC PLUS Exosome Purification Kit - for tissue culture media

EQPL10TC-1 10 reactions
EUR 694.8

ExoQuick Exosome Isolation and RNA Purification kit (for Serum and Plasma)

EQ806A-1 20 preps
EUR 664.8

ExoQuick Exosome Isolation and RNA Purification kit (for Tissue Culture Media)

EQ806TC-1 10 preps
EUR 664.8

SeraMir Exosome RNA Purification kit (5ml ExoQuick and 20 exoRNA columns)

RA806A-1 20 preps
EUR 664.8

Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL)

TMEXO-1 100 reactions
EUR 699.6

Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome)

P101EZ - Ask for price

ExoQuick TC 10ml

EXOTC10A-1 10 ml
EUR 478.8

ExoQuick TC 50ml

EXOTC50A-1 50 ml
EUR 1466.4

TMB for Precipitation

42R-TB100x 1000 ml
EUR 645.6
Description: High sensitivity TMB Substrate for Precipitation assays

DNA Precipitation Buffer

D106 55 ml
EUR 210
Description: DNA Precipitation Buffer by Cygnus Technologies is available in Europe via Gentaur.

Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome)

P100EZ - Ask for price

Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome) sample

P101EZ--sample - Ask for price

Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome) sample

P100EZ-sample - Ask for price

SeraMir Exosome RNA Purification kit (10ml ExoQuick-TC and 10 exoRNA columns) 10 preps

RA806TC-1 10 preps
EUR 664.8

Chicken IgY precipitation reagent

85R-1010 4 ml
EUR 664.8
Description: Chicken IgY precipitation reagent for use in immunoassays

PEG Virus Precipitation Kit

K904-200
EUR 1201.2

PEG Virus Precipitation Kit

K904-50
EUR 451.2

PEG Virus Precipitation Kit

K2190-200 200 preparations
EUR 1270.8

PEG Virus Precipitation Kit

K2190-50 50 preparations
EUR 486

50ML Protein Precipitation Kit

NAT1254 50ML
EUR 237.12

SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns)

RA800TC-1 10 reactions
EUR 790.8

Psoriasis Exosome

P141-PS - Ask for price

101Bio High Efficiency Protein Precipitation kit

P5W6 - Ask for price

In Vitro SDS-K+ Precipitation Kit

TG1010-1 100 assays
EUR 550.8

In Vitro SDS-K+ Precipitation Kit

TG1010-2 250 assays
EUR 685.2

In Vivo SDS-K+ Precipitation Kit

TG1011-1 100 assays
EUR 362.4

In Vivo SDS-K+ Precipitation Kit

TG1011-2 250 assays
EUR 496.8

Kinesis TELOS PPT Protein Precipitation Plate

CHR6509 EACH
EUR 84.3

Kinesis TELOS PPT Protein Precipitation Columns

CHR1378 PK100
EUR 190.41

Solution

S-2140-A 1ML
EUR 69.54

Leukemia - ALL Exosome

P141-ALL - Ask for price

Leukemia - AML Exosome

P141-AML - Ask for price

Bladder Cancer Exosome

P141-BL - Ask for price

Breast Cancer Exosome

P141-BR - Ask for price

Leukemia - CLL Exosome

P141-CLL - Ask for price

Leukemia - CML Exosome

P141-CML - Ask for price

Colorectal Cancer Exosome

P141-CO - Ask for price

Crohn's Disease Exosome

P141-CR - Ask for price

Kidney Cancer Exosome

P141-KN - Ask for price

Lung Cancer Exosome

P141-LG - Ask for price

Liver Cancer Exosome

P141-LV - Ask for price

Nasopharyngeal Cancer Exosome

P141-NA - Ask for price

Prostate Cancer Exosome

P141-PR - Ask for price

Rheumatoid Arthritis Exosome

P141-RA - Ask for price

Thyroid Cancer Exosome

P141-TH - Ask for price

Lyophilized Exosome Standards

ExoA375 100 μg
EUR 364.2

Lyophilized Exosome Standards

ExoHT29 100 μg
EUR 364.2

Lyophilized Exosome Standards

ExoMCF7 100 μg
EUR 364.2

Lyophilized Exosome Standards

ExoPC3 100 μg
EUR 364.2

Lyophilized Exosome Standards

ExoPLASMA 100 μg
EUR 364.2

Lyophilized Exosome Standards

ExoUrine 100 μg
EUR 364.2

Psoriasis Exosome RNA

P241-PS - Ask for price

Exosome Isolation kit (for serum & plasma -Exosome-RNA-NGS)

P101NGS - Ask for price

Sodium Pyruvate Solution, 100 mM Solution

CCM1753-100 100 mL
EUR 73.01

Membrane Protein Extraction Solution (Solution #1)

MPE01 40 ml
EUR 167.04
Description: This product includes 40 ml of Solution #1 for large-scale preparative extraction of membrane proteins (up to 130 mg) from the cell membranes.

Membrane Protein Extraction Solution (Solution #2)

MPE02 40 ml
EUR 167.04
Description: This product includes 40 ml of Solution #2 for large-scale preparative extraction of membrane proteins (up to 130 mg) from the cell membranes.

Membrane Protein Extraction Solution (Solution #3)

MPE03 40 ml
EUR 167.04
Description: This product includes 40 ml of Solution #3 for large-scale preparative extraction of membrane proteins (up to 130 mg) from the cell membranes.

Membrane Protein Extraction Solution (Solution #4)

MPE04 40 ml
EUR 167.04
Description: This product includes 40 ml of Solution #4 for large-scale preparative extraction of membrane proteins (up to 130 mg) from the cell membranes.

Membrane Protein Extraction Solution (Solution #5)

MPE05 40 ml
EUR 167.04
Description: This product includes 40 ml of Solution #5 for large-scale preparative extraction of membrane proteins (up to 130 mg) from the cell membranes.

Membrane Protein Extraction Solution (Solution #6)

MPE06 40 ml
EUR 167.04
Description: This product includes 40 ml of Solution #6 for large-scale preparative extraction of membrane proteins (up to 130 mg) from the cell membranes.

Membrane Protein Extraction Solution (Solution #7)

MPE07 40 ml
EUR 167.04
Description: This product includes 40 ml of Solution #7 for large-scale preparative extraction of membrane proteins (up to 130 mg) from the cell membranes.

Membrane Protein Extraction Solution (Solution #8)

MPE08 40 ml
EUR 167.04
Description: This product includes 40 ml of Solution #8 for large-scale preparative extraction of membrane proteins (up to 130 mg) from the cell membranes.

Membrane Protein Extraction Solution (Solution #9)

MPE09 40 ml
EUR 167.04
Description: This product includes 40 ml of Solution #9 for large-scale preparative extraction of membrane proteins (up to 130 mg) from the cell membranes.

Membrane Protein Extraction Solution (Solution #10)

MPE10 40 ml
EUR 167.04
Description: This product includes 40 ml of Solution #10 for large-scale preparative extraction of membrane proteins (up to 130 mg) from the cell membranes.

Membrane Protein Extraction Solution (Solution #11)

MPE11 40 ml
EUR 167.04
Description: This product includes 40 ml of Solution #11 for large-scale preparative extraction of membrane proteins (up to 130 mg) from the cell membranes.

Membrane Protein Extraction Solution (Solution #12)

MPE12 40 ml
EUR 167.04
Description: This product includes 40 ml of Solution #12 for large-scale preparative extraction of membrane proteins (up to 130 mg) from the cell membranes.

Bovine Anti-Chicken IgY Beads for immuno-precipitation

XG-6134 1 mL
EUR 546.72
Description: The anti-IgY beads were made by cross-linking of bovine IgG anti-IgY antibodies to Protein A/G agarose beads. IgY is the original designation of chicken IgG like immunoglobulin.

SDS Solution

2102-100
EUR 164.4

EDTA Solution

2103-100
EUR 164.4

TRIMMING SOLUTION

99-676-CM 1 L /pk
EUR 105.6
Description: Specialty Media; Islet products

Denaturing solution

DE540 1L
EUR 73.57

Borax Solution

BOR015 15 ml
EUR 67.2

Borax Solution

BOR125 125 ml
EUR 94.8

Borax Solution

BOR500 500 ml
EUR 142.8

Borax Solution

BOR999 1000 ml
EUR 219.6

Methenamine Solution

MET125 125 ml
EUR 76.8

Methenamine Solution

MET500 500 ml
EUR 114

Methenamine Solution

MET999 1000 ml
EUR 158.4

Orcein Solution

OAA125 125 ml
EUR 79.2

Orcein Solution

OAA500 500 ml
EUR 130.8

Orcein Solution

OAA999 1000 ml
EUR 177.6

Stop Solution

F006 12 ml
EUR 216
Description: Stop Solution by Cygnus Technologies is available in Europe via Gentaur.

Plug Solution

GR103038 500 mL
EUR 102

Prehybridization Solution

K2191050-1 5 ml
EUR 164.4
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Hybridization Solution

K2191050-2 5 ml
EUR 188.4
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Blocking Solution

K2191050-8 250 ul
EUR 164.4
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Schiff's Solution

SRF030 30 ml
EUR 76.8

Schiff's Solution

SRF250 250 ml
EUR 92.4

Schiff's Solution

SRF3800 1 Gal.
EUR 414

Schiff's Solution

SRF500 500 ml
EUR 123.6

Schiff's Solution

SRF999 1000 ml
EUR 178.8

Tartrazine Solution

TZQ125 125 ml
EUR 76.8

Tartrazine Solution

TZQ500 500 ml
EUR 117.6

Tartrazine Solution

TZQ999 1000 ml
EUR 152.4

Fortification Solution

5242-F 1ML
EUR 36.48

Fortification Solution

5252-FS 1ML
EUR 42.18

Decalcifying Solution

DECAL01 100ML
EUR 31.92

TISAB Solution

TISAB1L-500ML 500ML
EUR 33.06

TISAB Solution

TISAB5 5L
EUR 191.52

Chloroprene Solution

S-930A 1ML
EUR 128.82

DTT Solution 

R-1104-1 1 ml, 500 mM
EUR 48.4

DTT Solution 

R-1104
  • EUR 131.34
  • EUR 48.40
  • 1 ml, 500 mM
  • 1 ml, 500 mM

ExoQuick ULTRA EV Isolation Kit for Serum and Plasma

EQULTRA-20A-1 20 reactions
EUR 694.8

Exosome Component 9 Peptide

46-967P 0.1 mg
EUR 405.6
Description: (CT) PMSCL1 Peptide

Exosome Component 3 Protein

20-abx263422
  • EUR 276.00
  • EUR 1930.80
  • EUR 393.60
  • 1 µg
  • 50 ug
  • 5 ug

Exosome Component 1 Protein

20-abx263423
  • EUR 1930.80
  • EUR 393.60
  • EUR 276.00
  • 100 ug
  • 10 ug
  • 2 µg

Exosome Component 5 Protein

20-abx260873
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 25 ug
  • 5 ug

Exosome Component 7 Protein

20-abx261347
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

Exosome Component 10 Protein

20-abx261805
  • EUR 5238.00
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

Exosome Component 4 Protein

20-abx261954
  • EUR 5388.00
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

Exosome Component 9 Protein

20-abx262123
  • EUR 393.60
  • EUR 6106.80
  • EUR 276.00
  • 10 ug
  • 1 mg
  • 2 µg

Exosome Component 8 Protein

20-abx262850
  • EUR 393.60
  • EUR 7676.40
  • EUR 276.00
  • 10 ug
  • 1 mg
  • 2 µg

Exosome-TEM-easy Kit

P130 - Ask for price

Diabetes (Type 1) Exosome

P141-DB1 - Ask for price

Diabetes (Type 2) Exosome

P141-DB2 - Ask for price

Leukemia - Hairy Cell Exosome

P141-HC - Ask for price

Leukemia - Multiple Myeloma Exosome

P141-MM - Ask for price

Melanoma (skin cancer) Exosome

P141-MN - Ask for price

Non-Hodgkin lymphoma Exosome

P141-NH - Ask for price

Systemic Lupus Erythematosus Exosome

P141-SL - Ask for price

Uterine Cervical Cancer Exosome

P141-UC - Ask for price

Uterine Corpus Cancer Exosome

P141-UT - Ask for price

EXOCET Exosome Quantitation Kit

EXOCET96A-1 96 reactions
EUR 625.2

Fluorescent Exosome Standard (Urine )

ESF-15 100 µg
EUR 2067.6
Description: Fluorescent exosomes are labeled with green dye, which offer a stable fluorescent labeling.

FluoroCet Exosome Quantitation Kit

FCET96A-1 96 reactions
EUR 871.2

Normal human exosome RNA

P240 - Ask for price

Leukemia - ALL Exosome RNA

P241-ALL - Ask for price

Leukemia - AML Exosome RNA

P241-AML - Ask for price

Bladder Cancer Exosome RNA

P241-BL - Ask for price

Breast Cancer Exosome RNA

P241-BR - Ask for price

Leukemia - CLL Exosome RNA

P241-CLL - Ask for price

Leukemia - CML Exosome RNA

P241-CML - Ask for price

Colorectal Cancer Exosome RNA

P241-CO - Ask for price

Crohn's Disease Exosome RNA

P241-CR - Ask for price

Kidney Cancer Exosome RNA

P241-KN - Ask for price

Lung Cancer Exosome RNA

P241-LG - Ask for price

Liver Cancer Exosome RNA

P241-LV - Ask for price

Nasopharyngeal Cancer Exosome RNA

P241-NA - Ask for price

Prostate Cancer Exosome RNA

P241-PR - Ask for price

Rheumatoid Arthritis Exosome RNA

P241-RA - Ask for price

Thyroid Cancer Exosome RNA

P241-TH - Ask for price

Gentamicin Solution, Gentamicin Solution, 50 mg/mL

CCM1123-010 10 mL
EUR 76.86

ExoFectin Plasmid DNA-into-Exosome Kit (DNA transfection kit for exosome)

P402 - Ask for price

ExoQuick-TC ULTRA EV Isolation Kit for Tissue Culture Media

EQULTRA-20TC-1 20 reactions
EUR 694.8

PROTEINASE K SOLUTION

501-PK 5 ml
EUR 182.4
Description: Genomic PCR Grade Proteinase K Solution

2% Bisacrylamide Solution

A0114 500ml
EUR 78.79

Acrylamide 40% Solution

A0416-050 495ml
EUR 198

Acrylamide Solution 30%

A067-1L 1 L
EUR 123.6

Acrylamide Solution 30%

A067-500Ml 500 ml
EUR 85.2

CometAssay Lysis Solution

4250-010-01 100 mL
EUR 189.36

Ringer Solution Tablets

09-1025-100 100 tablets
EUR 84

HYGROMYCIN B SOLUTION

30-240-CR 20 mL/pk
EUR 188.4
Description: Media Catalog; Cell Culture Reagents

Propidium Iodide Solution

abx090616-100tests 100 tests
EUR 260.4

The exosome-loaded doxorubicin (Exo-Dox) is made by mixing exosome with Dox-HCl, desalinizing with triethylamine and then dialyzing against PBS overnight. Nanoparticle tracking analysis (NTA) and transmission electron microscopy (TEM) was used to characterize of exosome and Exo-Dox.