Urine miR-21-5p and miR-200c-3p as potential non-invasive biomarkers in patients with prostate cancer.

To evaluate miR-21-5p and miR-200C-3p expression in the urine of patients with prostate cancer (PCa) and to investigate their potential as biomarkers.The urine samples collected from 80 patients, including 20 patients who were diagnosed with benign prostatic hyperplasia ( BPH) and 60 patients diagnosed with PCa, were examined. The exosome isolation is done by using miRCURY exosome isolation kit (Exiqon, Denmark), total RNA was extracted using the miRCURY RNA Isolation Kit-Biofluid kit (Exiqon, Denmark), and complementary DNA (cDNA) was synthesized using a kit Universal cDNA Synthesis (Exiqon, Denmark ).

A quantitative polymerase chain reaction (qPCR) analysis of gene expression was performed using qPCR CFX 96 thermocycler (Bio-Rad). All procedures followed by manufacturers recommendations.The overexpressions of miR-21 in a group of non-metastatic PCa and metastatic PCa compared with BPH group was statistically significant with p-values ​​of 0.001 and 0.018, respectively. The non-metastatic PCa compared with metastatic PCa group was also statistically significant with a p-value of 0.037. Under the expression of miR-200C in non-metastatic PCa and PCa metastasis group compared to the BPH group was statistically significant with p-values ​​of 0.001 and 0.001, respectively.

The overexpressions of miR-21 was found in this study could be a potential non-invasive diagnostic tool for patients with PCa. Despite the significant results in our study, the use of micro-RNA in urine samples may vary due to epigenetic variation. Further studies with a larger population is needed to investigate the role of miR-21 and miR-200C as biomarkers in PCa.

 Urine miR-21-5p and miR-200c-3p as potential non-invasive biomarkers in patients with prostate cancer.
Urine miR-21-5p and miR-200c-3p as potential non-invasive biomarkers in patients with prostate cancer.

Circulating microRNAs in extracellular vesicles as a potential biomarker for psoriatic arthritis in patients with psoriasis.

Psoriatic arthritis (PsA) develops in ~ 30% of patients with psoriasis. The diagnosis of PsA challenging and there is no reliable molecular markers in clinical use. MicroRNAs are short noncoding regulatory RNAs, which can be actively packaged in extracellular vesicles (EV) and secreted into circulation.To investigate whether plasma-derived microRNAs EV can serve as a biomarker for PsA in patients with psoriasis.


METHOD
plasma samples obtained from patients with psoriasis the skin only (PSC) and patients with psoriasis and PsA. EVS plasma was isolated using Exosome miRCURYTM Isolation Kit. RNA sequencing was used to identify differentially expressed miRNAs EV in discovery phase (PSC, n = 15; PsA, n = 14). In the validation phase (PSC, n = 29; PsA, n = 28), 41 miRNAs been analyzed in plasma EVs by qPCR. Association of miRNAs identified with PsA assessed by logistic regression analysis.


RNA sequencing identified 19 miRNAs EV plasma levels differed significantly between PsA and PSC in the category of inventions. significantly lower levels of let-7b plasma EV-5p and miR-30e-5p in PsA vs. PSC confirmed in the validation cohort, and decreased their levels found to be associated with the presence of PsA. ROC analysis revealed AUC of 0.68 (95% CI 0.53 to 0.83) for the let-7b-5p and 0.69 (95% CI from 0.55 to 0.84) to the level of miR-30e-5p.Circulating EV microRNA altered in patients with PsA compared with the PSC. The findings of this exploratory study shows that circulating microRNAs EV can serve as a biomarker for arthritis in patients with psoriasis.


The main objective of this research is to develop nano-drug consisting of doxorubicin and exosome derived from mesenchymal stem cells, and to explore its effect on osteosarcoma in vitro.The exosomes isolated from bone marrow MSCs (BM-MSC) by Exosome Isolation Kit.

Exosome precipitation from Plasma + Thrombin
EPStep-T 5 ml
EUR 336
ExoQuick Exosome RNA Column Purification kit
EQ808A-1 20 preps
EUR 402
Exosome precipitation from Plasma and Serum
EPStep-PS 5 ml
EUR 297
ExoQuick-LP for lipoprotein pre-clear & exosome isolation
EXOLP5A-1 5 reactions
EUR 533
  • Category: Exosomes
PEG-it Virus Precipitation Solution (250 mL aliquot)
LV825A-1 250 ml
EUR 777
  • Category: Lentiviral Technology
PEG-it Virus Precipitation Solution (100 mL aliquot)
LV810A-1 100 ml
EUR 382
  • Category: Lentiviral Technology
ExoQuick PLUS Exosome Purification Kit - for serum and plasma
EQPL10A-1 10 reactions
EUR 579
  • Category: Exosomes
ExoQuick-CG cGMP-grade Exosome Isolation reagent (50 ml)
EXOCG50A-1 50 ml
EUR 1181
  • Category: Exosomes
ExoQuick-TC PLUS Exosome Purification Kit - for tissue culture media
EQPL10TC-1 10 reactions
EUR 579
  • Category: Exosomes
ExoQuick Exosome Isolation and RNA Purification kit (for Serum and Plasma)
EQ806A-1 20 preps
EUR 554
ExoQuick Exosome Isolation and RNA Purification kit (for Tissue Culture Media)
EQ806TC-1 10 preps
EUR 554
SeraMir Exosome RNA Purification kit (5ml ExoQuick and 20 exoRNA columns)
RA806A-1 20 preps
EUR 554
  • Category: MicroRNA Tools
Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL)
TMEXO-1 100 reactions
EUR 583
  • Category: Exosomes
Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome)
P101EZ NULL
EUR 0
ExoQuick TC 10ml
EXOTC10A-1 10 ml
EUR 399
  • Category: Exosomes
ExoQuick TC 50ml
EXOTC50A-1 50 ml
EUR 1222
  • Category: Exosomes
TMB for Precipitation
42R-TB100x 1000 ml
EUR 538
Description: High sensitivity TMB Substrate for Precipitation assays
DNA Precipitation Buffer
D106 55 ml
EUR 175
  • Product category: DNA and RNA extraction and purification
Description: DNA Precipitation Buffer by Cygnus Technologies is available in Europe via Gentaur.
Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome)
P100EZ NULL
EUR 0
Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome) sample
P101EZ--sample NULL
EUR 0
Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome) sample
P100EZ-sample NULL
EUR 0
SeraMir Exosome RNA Purification kit (10ml ExoQuick-TC and 10 exoRNA columns) 10 preps
RA806TC-1 10 preps
EUR 554
  • Category: MicroRNA Tools
Chicken IgY precipitation reagent
85R-1010 4 ml
EUR 554
Description: Chicken IgY precipitation reagent for use in immunoassays
PEG Virus Precipitation Kit
K2190-200 200 preparations
EUR 1059
PEG Virus Precipitation Kit
K2190-50 50 preparations
EUR 405
50ML Protein Precipitation Kit
NAT1254 50ML
EUR 225
PEG Virus Precipitation Kit
K904-200
EUR 1001
PEG Virus Precipitation Kit
K904-50
EUR 376
SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns)
RA800TC-1 10 reactions
EUR 659
  • Category: MicroRNA Tools
101Bio High Efficiency Protein Precipitation kit
P5W6 NULL
EUR 0
In Vitro SDS-K+ Precipitation Kit
TG1010-1 100 assays
EUR 459
In Vitro SDS-K+ Precipitation Kit
TG1010-2 250 assays
EUR 571
In Vivo SDS-K+ Precipitation Kit
TG1011-1 100 assays
EUR 302
In Vivo SDS-K+ Precipitation Kit
TG1011-2 250 assays
EUR 414
Psoriasis Exosome
P141-PS NULL
EUR 0
Sodium Pyruvate Solution, 100 mM Solution
CCM1753-100 100 mL
EUR 60.84
  • Product category: Culture Media
Lyophilized Exosome Standards
ExoA375 100 μg
EUR 303.5
Lyophilized Exosome Standards
ExoHT29 100 μg
EUR 303.5
Lyophilized Exosome Standards
ExoMCF7 100 μg
EUR 303.5
Lyophilized Exosome Standards
ExoPC3 100 μg
EUR 303.5
Lyophilized Exosome Standards
ExoPLASMA 100 μg
EUR 303.5
Lyophilized Exosome Standards
ExoUrine 100 μg
EUR 303.5
Leukemia - ALL Exosome
P141-ALL NULL
EUR 0
Leukemia - AML Exosome
P141-AML NULL
EUR 0
Bladder Cancer Exosome
P141-BL NULL
EUR 0
Breast Cancer Exosome
P141-BR NULL
EUR 0
Leukemia - CLL Exosome
P141-CLL NULL
EUR 0
Leukemia - CML Exosome
P141-CML NULL
EUR 0
Colorectal Cancer Exosome
P141-CO NULL
EUR 0
Crohn's Disease Exosome
P141-CR NULL
EUR 0
Kidney Cancer Exosome
P141-KN NULL
EUR 0
Lung Cancer Exosome
P141-LG NULL
EUR 0
Liver Cancer Exosome
P141-LV NULL
EUR 0
Nasopharyngeal Cancer Exosome
P141-NA NULL
EUR 0
Prostate Cancer Exosome
P141-PR NULL
EUR 0
Rheumatoid Arthritis Exosome
P141-RA NULL
EUR 0
Thyroid Cancer Exosome
P141-TH NULL
EUR 0
Psoriasis Exosome RNA
P241-PS NULL
EUR 0
Borax Solution
BOR015 15 ml
EUR 56
Borax Solution
BOR125 125 ml
EUR 79
Borax Solution
BOR500 500 ml
EUR 119
Borax Solution
BOR999 1000 ml
EUR 183
Stop Solution
F006 12 ml
EUR 180
  • Product category: Buffer solution
Description: Stop Solution by Cygnus Technologies is available in Europe via Gentaur.
TRIMMING SOLUTION
99-676-CM 1 L /pk
EUR 88
Description: Specialty Media; Islet products
SDS Solution
2102-100
EUR 137
EDTA Solution
2103-100
EUR 137
Prehybridization Solution
K2191050-1 5 ml
EUR 137
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Hybridization Solution
K2191050-2 5 ml
EUR 157
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Blocking Solution
K2191050-8 250 ul
EUR 137
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
AlphaBioCoat Solution
AC001 20 ml
EUR 271
Denaturing solution
DE540 1L
EUR 61.31
  • Product category: Electrophoresis Related/Reagents
Orcein Solution
OAA125 125 ml
EUR 66
Orcein Solution
OAA500 500 ml
EUR 109
Orcein Solution
OAA999 1000 ml
EUR 148
Methenamine Solution
MET125 125 ml
EUR 64
Methenamine Solution
MET500 500 ml
EUR 95
Methenamine Solution
MET999 1000 ml
EUR 132
DTT Solution
R-1104 1 ml, 500 mM
EUR 109.45
Description: fast delivery possible
Schiff's Solution
SRF030 30 ml
EUR 64
Schiff's Solution
SRF250 250 ml
EUR 77
Schiff's Solution
SRF3800 1 Gal.
EUR 345
Schiff's Solution
SRF500 500 ml
EUR 103
Schiff's Solution
SRF999 1000 ml
EUR 149
Tartrazine Solution
TZQ125 125 ml
EUR 64
Tartrazine Solution
TZQ500 500 ml
EUR 98
Tartrazine Solution
TZQ999 1000 ml
EUR 127
Exosome Isolation kit (for serum & plasma -Exosome-RNA-NGS)
P101NGS NULL
EUR 0
Gentamicin Solution, Gentamicin Solution, 50 mg/mL
CCM1123-010 10 mL
EUR 64.05
  • Product category: Culture Media
ExoQuick ULTRA EV Isolation Kit for Serum and Plasma
EQULTRA-20A-1 20 reactions
EUR 579
FluoroCet Exosome Quantitation Kit
FCET96A-1 96 reactions
EUR 726
  • Category: Exosomes
Fluorescent Exosome Standard (Urine )
ESF-15 100 µg
EUR 1723
Description: Fluorescent exosomes are labeled with green dye, which offer a stable fluorescent labeling.
EXOCET Exosome Quantitation Kit
EXOCET96A-1 96 reactions
EUR 521
  • Category: Exosomes
Exosome Component 5 Protein
20-abx260873
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 25 ug
  • 5 ug
  • Shipped within 5-10 working days.
Exosome Component 7 Protein
20-abx261347
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.
Exosome Component 10 Protein
20-abx261805
  • EUR 4365.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.
Exosome Component 4 Protein
20-abx261954
  • EUR 4490.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.
Exosome Component 9 Protein
20-abx262123
  • EUR 328.00
  • EUR 5089.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.
Exosome Component 8 Protein
20-abx262850
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.
Exosome Component 3 Protein
20-abx263422
  • EUR 230.00
  • EUR 1609.00
  • EUR 328.00
  • 1 µg
  • 50 ug
  • 5 ug
  • Shipped within 5-10 working days.
Exosome Component 1 Protein
20-abx263423
  • EUR 1609.00
  • EUR 328.00
  • EUR 230.00
  • 100 ug
  • 10 ug
  • 2 µg
  • Shipped within 5-10 working days.
Exosome-TEM-easy Kit
P130 NULL
EUR 0
Diabetes (Type 1) Exosome
P141-DB1 NULL
EUR 0
Diabetes (Type 2) Exosome
P141-DB2 NULL
EUR 0
Leukemia - Hairy Cell Exosome
P141-HC NULL
EUR 0
Leukemia - Multiple Myeloma Exosome
P141-MM NULL
EUR 0
Melanoma (skin cancer) Exosome
P141-MN NULL
EUR 0
Non-Hodgkin lymphoma Exosome
P141-NH NULL
EUR 0
Systemic Lupus Erythematosus Exosome
P141-SL NULL
EUR 0
Uterine Cervical Cancer Exosome
P141-UC NULL
EUR 0
Uterine Corpus Cancer Exosome
P141-UT NULL
EUR 0
Normal human exosome RNA
P240 NULL
EUR 0
Leukemia - ALL Exosome RNA
P241-ALL NULL
EUR 0
Leukemia - AML Exosome RNA
P241-AML NULL
EUR 0
Bladder Cancer Exosome RNA
P241-BL NULL
EUR 0
Breast Cancer Exosome RNA
P241-BR NULL
EUR 0
Leukemia - CLL Exosome RNA
P241-CLL NULL
EUR 0
Leukemia - CML Exosome RNA
P241-CML NULL
EUR 0
Colorectal Cancer Exosome RNA
P241-CO NULL
EUR 0
Crohn's Disease Exosome RNA
P241-CR NULL
EUR 0
Kidney Cancer Exosome RNA
P241-KN NULL
EUR 0
Lung Cancer Exosome RNA
P241-LG NULL
EUR 0
Liver Cancer Exosome RNA
P241-LV NULL
EUR 0
Nasopharyngeal Cancer Exosome RNA
P241-NA NULL
EUR 0
Prostate Cancer Exosome RNA
P241-PR NULL
EUR 0
Rheumatoid Arthritis Exosome RNA
P241-RA NULL
EUR 0
Thyroid Cancer Exosome RNA
P241-TH NULL
EUR 0
BSA Standard Solution
AJ642 1ml
EUR 60.44
  • Product category: Proteins/Protein Standards
Alcian Yellow Solution
ANY125 125 ml
EUR 83
Alcian Yellow Solution
ANY3800 1 Gal.
EUR 454
Alcian Yellow Solution
ANY500 500 ml
EUR 123
Alcian Yellow Solution
ANY999 1000 ml
EUR 178
HEPES, 1M Solution
CCM1291-100 100 mL
EUR 74.09
  • Product category: Culture Media
NEAA, Solution, 100X
CCM1668-100 100 mL
EUR 60.84
  • Product category: Culture Media
Phenol Red Solution
CA013-010 100ml
EUR 69
Sodium Pyruvate Solution
CA017-010 100ml
EUR 72
0.5M CaCl2 Solution
CA059-100 100ml
EUR 80
Formaldehyde 37% Solution
C5300-1 1L
EUR 72.19
  • Product category: Biochemicals/Biology Reagents/Protein Related
Tyloxapol (10% ) solution
B1159-250
EUR 512
Tyloxapol (10% ) solution
B1159-50
EUR 175
Hematoxylin Counterstain Solution
AR0005 12mL(for 120-180 assays)
EUR 65
Ponceau S solution
AR0142 12mL(10X)
EUR 60
Astra Blue Solution
AST125 125 ml
EUR 104
Astra Blue Solution
AST500 500 ml
EUR 183
MgCl2 Solution, 50mM
BIO-37026 3 x 1.2ml Ask for price
Proteinase K Solution
BIO-37084 5ml @ 20mg/ml Ask for price
Proteinase K Solution
BIO-37085 5 x 5ml @ 20mg/ml Ask for price
Cell Detachment Solution
ADF001 50 ml
EUR 114
Universal Detachment Solution
AD002 100 ml
EUR 148
Hematoxylin Solution (5%)
HSV250 250 ml
EUR 133
Hematoxylin Solution (5%)
HSV500 500 ml
EUR 183
Hematoxylin Solution (5%)
HSV999 1000 ml
EUR 277
Gentian Violet Solution
GVS125 125 ml
EUR 84
Gentian Violet Solution
GVS500 500 ml
EUR 130
Gentian Violet Solution
GVS999 1000 ml
EUR 178
Proteinase K solution
GZ5904-100ML 100 ml
EUR 907
Proteinase K solution
GZ5904-25ML 25 ml
EUR 349
Sodium silicate solution
GK8776-100ML 100 ml
EUR 40

The exosome-loaded doxorubicin (Exo-Dox) is made by mixing exosome with Dox-HCl, desalinizing with triethylamine and then dialyzing against PBS overnight. Nanoparticle tracking analysis (NTA) and transmission electron microscopy (TEM) was used to characterize of exosome and Exo-Dox.