To evaluate miR-21-5p and miR-200C-3p expression in the urine of patients with prostate cancer (PCa) and to investigate their potential as biomarkers.The urine samples collected from 80 patients, including 20 patients who were diagnosed with benign prostatic hyperplasia ( BPH) and 60 patients diagnosed with PCa, were examined. The exosome isolation is done by using miRCURY exosome isolation kit (Exiqon, Denmark), total RNA was extracted using the miRCURY RNA Isolation Kit-Biofluid kit (Exiqon, Denmark), and complementary DNA (cDNA) was synthesized using a kit Universal cDNA Synthesis (Exiqon, Denmark ).
A quantitative polymerase chain reaction (qPCR) analysis of gene expression was performed using qPCR CFX 96 thermocycler (Bio-Rad). All procedures followed by manufacturers recommendations.The overexpressions of miR-21 in a group of non-metastatic PCa and metastatic PCa compared with BPH group was statistically significant with p-values of 0.001 and 0.018, respectively. The non-metastatic PCa compared with metastatic PCa group was also statistically significant with a p-value of 0.037. Under the expression of miR-200C in non-metastatic PCa and PCa metastasis group compared to the BPH group was statistically significant with p-values of 0.001 and 0.001, respectively.
The overexpressions of miR-21 was found in this study could be a potential non-invasive diagnostic tool for patients with PCa. Despite the significant results in our study, the use of micro-RNA in urine samples may vary due to epigenetic variation. Further studies with a larger population is needed to investigate the role of miR-21 and miR-200C as biomarkers in PCa.
Urine miR-21-5p and miR-200c-3p as potential non-invasive biomarkers in patients with prostate cancer.
Circulating microRNAs in extracellular vesicles as a potential biomarker for psoriatic arthritis in patients with psoriasis.
Psoriatic arthritis (PsA) develops in ~ 30% of patients with psoriasis. The diagnosis of PsA challenging and there is no reliable molecular markers in clinical use. MicroRNAs are short noncoding regulatory RNAs, which can be actively packaged in extracellular vesicles (EV) and secreted into circulation.To investigate whether plasma-derived microRNAs EV can serve as a biomarker for PsA in patients with psoriasis.
METHOD plasma samples obtained from patients with psoriasis the skin only (PSC) and patients with psoriasis and PsA. EVS plasma was isolated using Exosome miRCURYTM Isolation Kit. RNA sequencing was used to identify differentially expressed miRNAs EV in discovery phase (PSC, n = 15; PsA, n = 14). In the validation phase (PSC, n = 29; PsA, n = 28), 41 miRNAs been analyzed in plasma EVs by qPCR. Association of miRNAs identified with PsA assessed by logistic regression analysis.
RNA sequencing identified 19 miRNAs EV plasma levels differed significantly between PsA and PSC in the category of inventions. significantly lower levels of let-7b plasma EV-5p and miR-30e-5p in PsA vs. PSC confirmed in the validation cohort, and decreased their levels found to be associated with the presence of PsA. ROC analysis revealed AUC of 0.68 (95% CI 0.53 to 0.83) for the let-7b-5p and 0.69 (95% CI from 0.55 to 0.84) to the level of miR-30e-5p .Circulating EV microRNA altered in patients with PsA compared with the PSC. The findings of this exploratory study shows that circulating microRNAs EV can serve as a biomarker for arthritis in patients with psoriasis.
The main objective of this research is to develop nano-drug consisting of doxorubicin and exosome derived from mesenchymal stem cells, and to explore its effect on osteosarcoma in vitro.The exosomes isolated from bone marrow MSCs (BM-MSC) by Exosome Isolation Kit.
PEG-it Virus Precipitation Solution (250 mL aliquot)
LV825A-1
SBI
250 ml
EUR 711
TMB for Precipitation
42R-TB100x
Fitzgerald
1000 ml
EUR 473
Description: High sensitivity TMB Substrate for Precipitation assays
TMB for Precipitation
MBS538888-1L
MyBiosource
1L
EUR 620
TMB for Precipitation
MBS538888-5x1L
MyBiosource
5x1L
EUR 2700
DNA Precipitation Buffer
D106
Cygnus Technologies
55 ml
EUR 210
Description: DNA Precipitation Buffer by Cygnus Technologies is available in Europe via Gentaur.
ExoQuick Exosome RNA Column Purification kit
EQ808A-1
SBI
20 preps
EUR 344
PEG Virus Precipitation Kit
K2190-200
ApexBio
200 preparations
EUR 1270.8
PEG Virus Precipitation Kit
K2190-50
ApexBio
50 preparations
EUR 582.4
Description: Kits|Tools Kit#Tools Kit|Virus Concentration Kit#Kits
PEG Virus Precipitation Kit
K904-200
Biovision
each
EUR 1201.2
PEG Virus Precipitation Kit
K904-50
Biovision
each
EUR 451.2
Lentivirus Precipitation Reagent
VC125
ALSTEM
250 ml
EUR 432.6
Lentivirus Precipitation Reagent
VC150
ALSTEM
500 ml
EUR 814.8
Retrovirus Precipitation Reagent
VC200
ALSTEM
100 ml
EUR 194.6
Retrovirus Precipitation Reagent
VC225
ALSTEM
250 ml
EUR 432.6
Retrovirus Precipitation Reagent
VC250
ALSTEM
500 ml
EUR 814.8
Lentivirus Precipitation Reagent
VC100
ALSTEM
100 ml
EUR 195.3
ExoQuick-LP for lipoprotein pre-clear & exosome isolation
EXOLP5A-1
SBI
5 reactions
EUR 473
Chicken IgY precipitation reagent
85R-1010
Fitzgerald
4 ml
EUR 638
Description: Chicken IgY precipitation reagent for use in immunoassays
Chicken IgY precipitation reagent
MBS5307794-2mL
MyBiosource
2mL
EUR 975
Chicken IgY precipitation reagent
MBS5307794-5x2mL
MyBiosource
5x2mL
EUR 4245
In Vivo SDS-K+ Precipitation Kit
TG1011-1
TopoGen
100 assays
EUR 362.4
In Vivo SDS-K+ Precipitation Kit
TG1011-2
TopoGen
250 assays
EUR 496.8
Nucleic Acid Precipitation Enhancer
TBS6010
Tribioscience
2 x0.5 mL
EUR 98
In Vitro SDS-K+ Precipitation Kit
TG1010-1
TopoGen
100 assays
EUR 550.8
In Vitro SDS-K+ Precipitation Kit
TG1010-2
TopoGen
250 assays
EUR 685.2
PEG Virus Precipitation Kit - 200 preparations
GWB-AXR384
GenWay Biotech
200 preparations
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PEG Virus Precipitation Kit - 50 preparations
GWB-AXR385
GenWay Biotech
50 preparations
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101Bio High Efficiency Protein Precipitation kit
P5W6
101Bio
30 ml / 30 ml
EUR 129
LPA (Linear Polyacrylamide) for DNA/RNA Precipitation
10510000-1
Bio-WORLD
10 mg
EUR 105.44
LPA (Linear Polyacrylamide) for DNA/RNA Precipitation
10510000-2
Bio-WORLD
25 mg
EUR 172.85
Minute TM High Efficiency Protein Precipitation kit
WA-006
Inventbiotech
each
EUR 160
ExoQuick-CG cGMP-grade Exosome Isolation reagent (50 ml)
EXOCG50A-1
SBI
50 ml
EUR 1106
ExoQuick PLUS Exosome Purification Kit - for serum and plasma
EQPL10A-1
SBI
10 reactions
EUR 518
ExoQuick Exosome Isolation and RNA Purification kit (for Serum and Plasma)
EQ806A-1
SBI
20 preps
EUR 493
ExoQuick Exosome Isolation and RNA Purification kit (for Tissue Culture Media)
EQ806TC-1
SBI
10 preps
EUR 493
MinuteTM Native Protein Precipitation Kit from Saliva (20 tests)
SV-043
Inventbiotech
each
EUR 330
ExoQuick-TC PLUS Exosome Purification Kit - for tissue culture media
EQPL10TC-1
SBI
10 reactions
EUR 518
SeraMir Exosome RNA Purification kit (5ml ExoQuick and 20 exoRNA columns)
RA806A-1
SBI
20 preps
EUR 493
SeraMir Exosome RNA Purification kit (10ml ExoQuick-TC and 10 exoRNA columns) 10 preps
RA806TC-1
SBI
10 preps
EUR 493
SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns)
RA800TC-1
SBI
10 reactions
EUR 595
Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome)
P101EZ
101Bio
-
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Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome)
P100EZ
101Bio
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Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome) sample
P101EZ--sample
101Bio
-
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ExoQuick TC 10ml
EXOTC10A-1
SBI
10 ml
EUR 341
ExoQuick TC 50ml
EXOTC50A-1
SBI
50 ml
EUR 1147
Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome) sample
P100EZ-sample
101Bio
-
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Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800TC-1 with 50ml ExoQuick-TC, RA805A-1 and RA812A-1 components)
RA822TC-1
SBI
20 profiles
EUR 1533
ExoQuick ULTRA EV Isolation Kit for Serum and Plasma
EQULTRA-20A-1
SBI
20 reactions
EUR 941
Universal Protein Precipitating Reagent
BSP012
Bio Basic
100preps
EUR 97.58
ExoQuick-TC ULTRA EV Isolation Kit for Tissue Culture Media
EQULTRA-20TC-1
SBI
20 reactions
EUR 518
ExoQualiTM Exosome DNA Extraction Kit (Pre-Isolated Exosomes)
CEIK-050L-30T
Creative Bioarray
30T
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ExoQualiTM Exosome DNA Extraction Kit (Pre-Isolated Exosomes)
CEIK-050L-50T
Creative Bioarray
50T
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ExoQualiTM Exosome RNA Extraction Kit (Pre-Isolated Exosomes)
CEIK-051L-30T
Creative Bioarray
30T
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ExoQualiTM Exosome RNA Extraction Kit (Pre-Isolated Exosomes)
CEIK-051L-50T
Creative Bioarray
50T
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Sulfur Precipitated pure, 98%
69181
Sisco Laboratories
500 Gms
EUR 1.73
Description: Part B
Silicic acid, precipitated
GRM622-500G
EWC Diagnostics
1 unit
EUR 13.55
Description: Silicic acid, precipitated
Sulphur precipitated, Hi-AR™
RM1316-500G
EWC Diagnostics
1 unit
EUR 11.59
Description: Sulphur precipitated, Hi-AR™
Calcium resinate (precipitated)
C01800
Pfaltz & Bauer
25G
EUR 113.58
Description: CAS N° 9007-13-0
ExoQualiTM Exosome Isolation Kit (Urine)
CEIK-003L-10T
Creative Bioarray
10 T
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ExoQualiTM Exosome Isolation Kit (Urine)
CEIK-003L-30T
Creative Bioarray
30 T
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ExoQualiTM Exosome Isolation Kit (Serum)
CEIK-005L-10T
Creative Bioarray
10 T
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ExoQualiTM Exosome Isolation Kit (Serum)
CEIK-005L-30T
Creative Bioarray
30 T
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ExoQualiTM Exosome Isolation Kit (Plasma)
CEIK-004L-10T
Creative Bioarray
10 T
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ExoQualiTM Exosome Isolation Kit (Plasma)
CEIK-004L-30T
Creative Bioarray
30 T
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ExoQualiTM Exosome Chromatography Columns
ESEC-001L-5columns
Creative Bioarray
5 columns
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Magnesium stearate precipitated fine pow
GRM7219-500G
EWC Diagnostics
1 unit
EUR 6.93
Description: Magnesium stearate precipitated fine pow
Rabbit plasma, barium chloride precipitate
MBS136153-500mL
MyBiosource
500mL
EUR 1360
Rabbit plasma, barium chloride precipitate
MBS136153-50mL
MyBiosource
50mL
EUR 270
Rabbit plasma, barium chloride precipitate
MBS136153-5x500mL
MyBiosource
5x500mL
EUR 5930
Calcium carbonate, 99%, precipitated, Ph. Eur., BP, USP, FCC grade
GX0868
Glentham Life Sciences
1kg
EUR 30.35
ExoQualiTM Exosome Isolation Kit (Cell Media)
CEIK-001L-10T
Creative Bioarray
10 T
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ExoQualiTM Exosome Isolation Kit (Cell Media)
CEIK-001L-30T
Creative Bioarray
30 T
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ExoQualiTM Exosome Isolation Kit (All in one)
CEIK-008L-10T
Creative Bioarray
10 T
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ExoQualiTM Exosome Isolation Kit (All in one)
CEIK-008L-30T
Creative Bioarray
30 T
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ExoQualiTM Max Exosome Chromatography Columns
ESEC-002L-3columns
Creative Bioarray
3 columns
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ExoQualiTM Exosome Isolation Kit (Plasma & Serum)
CEIK-006L-10T
Creative Bioarray
10 T
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ExoQualiTM Exosome Isolation Kit (Plasma & Serum)
CEIK-006L-30T
Creative Bioarray
30 T
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ExoQualiTM Mini Exosome Chromatography Columns
ESEC-003L-2columns
Creative Bioarray
2 columns
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ExoQualiTM Exosome Isolation Kit (Stem Cell Media)
CEIK-002L-10T
Creative Bioarray
10 T
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ExoQualiTM Exosome Isolation Kit (Stem Cell Media)
CEIK-002L-30T
Creative Bioarray
30 T
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ExoQualiTM Exosome Isolation Kit (other body fluids)
CEIK-007L-10T
Creative Bioarray
10 T
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ExoQualiTM Exosome Isolation Kit (other body fluids)
CEIK-007L-30T
Creative Bioarray
30 T
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ExoQualiTM Exosome DNA Extraction Kit (Plasma & Serum)
CEIK-048L-30T
Creative Bioarray
30T
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ExoQualiTM Exosome DNA Extraction Kit (Plasma & Serum)
CEIK-048L-50T
Creative Bioarray
50T
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ExoQualiTM Exosome RNA Extraction Kit (Plasma & Serum)
CEIK-093L-30T
Creative Bioarray
30T
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ExoQualiTM Exosome RNA Extraction Kit (Plasma & Serum)
CEIK-093L-50T
Creative Bioarray
50T
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ExoQuali™ Lyophilized Exosome Standards ( Human Serum )
CEIK-070L-2vials1x1010particlespervial
Creative Bioarray
2 vials (> 1x10^10 particles per vial)
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ExoQuali™ Lyophilized Exosome Standards ( Human Serum )
CEIK-070L-5vials1x1010particlespervial
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5 vials (> 1x10^10 particles per vial)
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ExoQuali™ Lyophilized Exosome Standards ( Human Urine )
CEIK-071L-2vials1x1010particlespervial
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ExoQuali™ Lyophilized Exosome Standards ( Human Urine )
CEIK-071L-5vials1x1010particlespervial
Creative Bioarray
5 vials (> 1x10^10 particles per vial)
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ExoQuali™ Lyophilized Exosome Standards ( Human Plasma )
CEIK-068L-2vials1x1010particlespervial
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2 vials (> 1x10^10 particles per vial)
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ExoQuali™ Lyophilized Exosome Standards ( Human Plasma )
CEIK-068L-5vials1x1010particlespervial
Creative Bioarray
5 vials (> 1x10^10 particles per vial)
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ExoQuali™ Lyophilized Exosome Standards ( Human Saliva )
CEIK-069L-2vials1x1010particlespervial
Creative Bioarray
2 vials (> 1x10^10 particles per vial)
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ExoQuali™ Lyophilized Exosome Standards ( Human Saliva )
CEIK-069L-5vials1x1010particlespervial
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ExoQualiTM Overall Exosome Isolation Reagent (from serum)
CEIK-044L-30T
Creative Bioarray
30 T
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ExoQualiTM Overall Exosome Isolation Reagent (from serum)
CEIK-044L-50T
Creative Bioarray
50 T
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ExoQualiTM Overall Exosome Isolation Reagent (from urine)
CEIK-046L-10T
Creative Bioarray
10 T
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ExoQualiTM Overall Exosome Isolation Reagent (from urine)
CEIK-046L-24T
Creative Bioarray
24 T
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ExoQuali™ Lyophilized Exosome Standards ( MM1 cell line )
CEIK-064L-2vials1x1010particlespervial
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ExoQuali™ Lyophilized Exosome Standards ( MM1 cell line )
CEIK-064L-5vials1x1010particlespervial
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ExoQuali™ Lyophilized Exosome Standards ( PC3 cell line )
CEIK-065L-2vials1x1010particlespervial
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2 vials (> 1x10^10 particles per vial)
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ExoQuali™ Lyophilized Exosome Standards ( PC3 cell line )
CEIK-065L-5vials1x1010particlespervial
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5 vials (> 1x10^10 particles per vial)
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ExoQualiTM Overall Exosome Isolation Reagent (from plasma)
CEIK-045L-30T
Creative Bioarray
30 T
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ExoQualiTM Overall Exosome Isolation Reagent (from plasma)
CEIK-045L-50T
Creative Bioarray
50 T
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ExoQuali™ Lyophilized Exosome Standards ( A549 cell line )
CEIK-054L-2vials1x1010particlespervial
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ExoQuali™ Lyophilized Exosome Standards ( A549 cell line )
CEIK-054L-5vials1x1010particlespervial
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ExoQualiTM Total Exosome Capture & Quantification Kit (Serum)
CEIK-107L-96wells
Creative Bioarray
96 wells
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ExoQualiTM Total Exosome Capture & Quantification Kit (Urine)
CEIK-110L-96wells
Creative Bioarray
96 wells
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ExoQuali™ Lyophilized Exosome Standards ( COLO1 cell line )
CEIK-058L-2vials1x1010particlespervial
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ExoQuali™ Lyophilized Exosome Standards ( COLO1 cell line )
CEIK-058L-5vials1x1010particlespervial
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ExoQualiTM Total Exosome Capture & Quantification Kit (Plasma)
CEIK-108L-96wells
Creative Bioarray
96 wells
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ExoQuali™ Lyophilized Exosome Standards ( U87 MG cell line )
CEIK-067L-2vials1x1010particlespervial
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ExoQuali™ Lyophilized Exosome Standards ( U87 MG cell line )
CEIK-067L-5vials1x1010particlespervial
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ExoQuali™ Lyophilized Exosome Standards ( B16F10 cell line )
CEIK-055L-2vials1x1010particlespervial
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ExoQuali™ Lyophilized Exosome Standards ( B16F10 cell line )
CEIK-055L-5vials1x1010particlespervial
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ExoQuali™ Lyophilized Exosome Standards ( BLCL21 cell line )
CEIK-056L-2vials1x1010particlespervial
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ExoQuali™ Lyophilized Exosome Standards ( BLCL21 cell line )
CEIK-056L-5vials1x1010particlespervial
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ExoQuali™ Lyophilized Exosome Standards ( HCT116 cell line )
CEIK-060L-2vials1x1010particlespervial
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ExoQuali™ Lyophilized Exosome Standards ( HCT116 cell line )
CEIK-060L-5vials1x1010particlespervial
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ExoQuali™ Lyophilized Exosome Standards ( BPH-1 cell line )
CEIK-057L-2vials1x1010particlespervial
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ExoQuali™ Lyophilized Exosome Standards ( BPH-1 cell line )
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ExoQuali™ Lyophilized Exosome Standards ( K-562 cell line )
CEIK-062L-2vials1x1010particlespervial
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ExoQuali™ Lyophilized Exosome Standards ( K-562 cell line )
CEIK-062L-5vials1x1010particlespervial
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ExoQualiTM Overall Exosome Isolation Immunoplates (from Serum)
CEIK-023L-96wells
Creative Bioarray
96 wells
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ExoQualiTM Overall Exosome Isolation Immunoplates (from Saliva)
CEIK-026L-96wells
Creative Bioarray
96 wells
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ExoQualiTM Overall Exosome Isolation Immunoplates (from Plasma)
CEIK-095L-96wells
Creative Bioarray
96 wells
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ExoQualiTM Overall Exosome Isolation Immunoplates (from Urine)
CEIK-096L-96wells
Creative Bioarray
96 wells
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ExoQualiTM Exosome DNA Extraction Kit (Urine & Cell Supernatant)
CEIK-049L-10T
Creative Bioarray
10T
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ExoQualiTM Exosome DNA Extraction Kit (Urine & Cell Supernatant)
CEIK-049L-20T
Creative Bioarray
20T
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ExoQualiTM Exosome RNA Extraction Kit (Urine & Cell Supernatant)
CEIK-094L-10T
Creative Bioarray
10T
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ExoQualiTM Exosome RNA Extraction Kit (Urine & Cell Supernatant)
CEIK-094L-20T
Creative Bioarray
20T
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ExoQuali™ Lyophilized Exosome Standards ( SK-N-SH cell line )
CEIK-066L-2vials1x1010particlespervial
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ExoQuali™ Lyophilized Exosome Standards ( SK-N-SH cell line )
CEIK-066L-5vials1x1010particlespervial
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*Innovative Grade US Origin Rabbit Barium Chloride Precipitate Plasma Lyophilized
IRBBACL2PPLALY500ML
Innovative research
each
EUR 1045
Description: *Innovative Grade US Origin Rabbit Barium Chloride Precipitate Plasma Lyophilized
*Innovative Grade US Origin Rabbit Barium Chloride Precipitate Plasma Lyophilized
IRBBACL2PPLALY50ML
Innovative research
each
EUR 150
Description: *Innovative Grade US Origin Rabbit Barium Chloride Precipitate Plasma Lyophilized
ExoQualiTM Overall Exosome Isolation Immunoplates (from Cell Media)
CEIK-029L-96wells
Creative Bioarray
96 wells
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ExoQualiTM Overall Exosome Isolation Reagent (from other body fluids)
CEIK-047L-10T
Creative Bioarray
10 T
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ExoQualiTM Overall Exosome Isolation Reagent (from other body fluids)
CEIK-047L-24T
Creative Bioarray
24 T
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ExoQualiTM Total Exosome Capture & Quantification Kit (Cell Supernatant)
CEIK-109L-96wells
Creative Bioarray
96 wells
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ExoQualiTM Overall Exosome Isolation Reagent (from cell culture media)
CEIK-043L-10T
Creative Bioarray
10 T
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ExoQualiTM Overall Exosome Isolation Reagent (from cell culture media)
CEIK-043L-24T
Creative Bioarray
24 T
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ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Cell media, Colorimetric)
K1205-100
Biovision
each
EUR 1161.6
ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Cell Media, Luminometric)
K1210-100
Biovision
each
EUR 1161.6
ExoQualiTM Human Exosome Capture and Isolation Kits (for Serum/Plasma) (CD9 Capture Beads)
CEIK-101L-10T
Creative Bioarray
10T
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The exosome-loaded doxorubicin (Exo-Dox) is made by mixing exosome with Dox-HCl, desalinizing with triethylamine and then dialyzing against PBS overnight. Nanoparticle tracking analysis (NTA) and transmission electron microscopy (TEM) was used to characterize of exosome and Exo-Dox.