Urine miR-21-5p and miR-200c-3p as potential non-invasive biomarkers in patients with prostate cancer.

To evaluate miR-21-5p and miR-200C-3p expression in the urine of patients with prostate cancer (PCa) and to investigate their potential as biomarkers.The urine samples collected from 80 patients, including 20 patients who were diagnosed with benign prostatic hyperplasia ( BPH) and 60 patients diagnosed with PCa, were examined. The exosome isolation is done by using miRCURY exosome isolation kit (Exiqon, Denmark), total RNA was extracted using the miRCURY RNA Isolation Kit-Biofluid kit (Exiqon, Denmark), and complementary DNA (cDNA) was synthesized using a kit Universal cDNA Synthesis (Exiqon, Denmark ).

A quantitative polymerase chain reaction (qPCR) analysis of gene expression was performed using qPCR CFX 96 thermocycler (Bio-Rad). All procedures followed by manufacturers recommendations.The overexpressions of miR-21 in a group of non-metastatic PCa and metastatic PCa compared with BPH group was statistically significant with p-values ​​of 0.001 and 0.018, respectively. The non-metastatic PCa compared with metastatic PCa group was also statistically significant with a p-value of 0.037. Under the expression of miR-200C in non-metastatic PCa and PCa metastasis group compared to the BPH group was statistically significant with p-values ​​of 0.001 and 0.001, respectively.

The overexpressions of miR-21 was found in this study could be a potential non-invasive diagnostic tool for patients with PCa. Despite the significant results in our study, the use of micro-RNA in urine samples may vary due to epigenetic variation. Further studies with a larger population is needed to investigate the role of miR-21 and miR-200C as biomarkers in PCa.

 Urine miR-21-5p and miR-200c-3p as potential non-invasive biomarkers in patients with prostate cancer.
Urine miR-21-5p and miR-200c-3p as potential non-invasive biomarkers in patients with prostate cancer.

Circulating microRNAs in extracellular vesicles as a potential biomarker for psoriatic arthritis in patients with psoriasis.

Psoriatic arthritis (PsA) develops in ~ 30% of patients with psoriasis. The diagnosis of PsA challenging and there is no reliable molecular markers in clinical use. MicroRNAs are short noncoding regulatory RNAs, which can be actively packaged in extracellular vesicles (EV) and secreted into circulation.To investigate whether plasma-derived microRNAs EV can serve as a biomarker for PsA in patients with psoriasis.


METHOD
plasma samples obtained from patients with psoriasis the skin only (PSC) and patients with psoriasis and PsA. EVS plasma was isolated using Exosome miRCURYTM Isolation Kit. RNA sequencing was used to identify differentially expressed miRNAs EV in discovery phase (PSC, n = 15; PsA, n = 14). In the validation phase (PSC, n = 29; PsA, n = 28), 41 miRNAs been analyzed in plasma EVs by qPCR. Association of miRNAs identified with PsA assessed by logistic regression analysis.


RNA sequencing identified 19 miRNAs EV plasma levels differed significantly between PsA and PSC in the category of inventions. significantly lower levels of let-7b plasma EV-5p and miR-30e-5p in PsA vs. PSC confirmed in the validation cohort, and decreased their levels found to be associated with the presence of PsA. ROC analysis revealed AUC of 0.68 (95% CI 0.53 to 0.83) for the let-7b-5p and 0.69 (95% CI from 0.55 to 0.84) to the level of miR-30e-5p.Circulating EV microRNA altered in patients with PsA compared with the PSC. The findings of this exploratory study shows that circulating microRNAs EV can serve as a biomarker for arthritis in patients with psoriasis.


The main objective of this research is to develop nano-drug consisting of doxorubicin and exosome derived from mesenchymal stem cells, and to explore its effect on osteosarcoma in vitro.The exosomes isolated from bone marrow MSCs (BM-MSC) by Exosome Isolation Kit.

PEG-it Virus Precipitation Solution (100 mL aliquot)

LV810A-1 100 ml
EUR 325

TMB for Precipitation

42R-TB100x 1000 ml
EUR 645.6
Description: High sensitivity TMB Substrate for Precipitation assays

ExoQuick Exosome RNA Column Purification kit

EQ808A-1 20 preps
EUR 344

DNA Precipitation Buffer

D106 55 ml
EUR 210
Description: DNA Precipitation Buffer by Cygnus Technologies is available in Europe via Gentaur.

PEG Virus Precipitation Kit

K904-200 each
EUR 1201.2

PEG Virus Precipitation Kit

K904-50 each
EUR 451.2

PEG Virus Precipitation Kit

K2190-200 200 preparations
EUR 1270.8

PEG Virus Precipitation Kit

K2190-50 50 preparations
EUR 486

ExoQuick-LP for lipoprotein pre-clear & exosome isolation

EXOLP5A-1 5 reactions
EUR 473

50ML Protein Precipitation Kit

NAT1254 50ML
EUR 309.6

Chicken IgY precipitation reagent

85R-1010 4 ml
EUR 664.8
Description: Chicken IgY precipitation reagent for use in immunoassays

In Vivo SDS-K+ Precipitation Kit

TG1011-1 100 assays
EUR 362.4

In Vivo SDS-K+ Precipitation Kit

TG1011-2 250 assays
EUR 496.8

In Vitro SDS-K+ Precipitation Kit

TG1010-1 100 assays
EUR 550.8

In Vitro SDS-K+ Precipitation Kit

TG1010-2 250 assays
EUR 685.2

Kinesis TELOS PPT Protein Precipitation Plate

CHR6509 EACH
EUR 105.75

Kinesis TELOS PPT Protein Precipitation Columns

CHR1378 PK100
EUR 222

101Bio High Efficiency Protein Precipitation kit

P5W6 - Ask for price

ExoQuick-CG cGMP-grade Exosome Isolation reagent (50 ml)

EXOCG50A-1 50 ml
EUR 1106

ExoQuick PLUS Exosome Purification Kit - for serum and plasma

EQPL10A-1 10 reactions
EUR 518

ExoQuick Exosome Isolation and RNA Purification kit (for Serum and Plasma)

EQ806A-1 20 preps
EUR 493

ExoQuick Exosome Isolation and RNA Purification kit (for Tissue Culture Media)

EQ806TC-1 10 preps
EUR 493

ExoQuick-TC PLUS Exosome Purification Kit - for tissue culture media

EQPL10TC-1 10 reactions
EUR 518

SeraMir Exosome RNA Purification kit (5ml ExoQuick and 20 exoRNA columns)

RA806A-1 20 preps
EUR 493

SeraMir Exosome RNA Purification kit (10ml ExoQuick-TC and 10 exoRNA columns) 10 preps

RA806TC-1 10 preps
EUR 493

SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns)

RA800TC-1 10 reactions
EUR 595

Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome)

P101EZ - Ask for price

Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome)

P100EZ - Ask for price

Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome) sample

P101EZ--sample - Ask for price

Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome) sample

P100EZ-sample - Ask for price

ExoQuick TC 10ml

EXOTC10A-1 10 ml
EUR 341

ExoQuick TC 50ml

EXOTC50A-1 50 ml
EUR 1147

Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800TC-1 with 50ml ExoQuick-TC, RA805A-1 and RA812A-1 components)

RA822TC-1 20 profiles
EUR 1533

TMB Precipitating (High Sensitivity)

TM5125 125 ml
EUR 102

TMB Precipitating (High Sensitivity)

TM5500 500 ml
EUR 165.6

TMB Precipitating (High Sensitivity)

TM5999 1000 ml
EUR 249.6

TMB Precipitating Reagent (For Membranes)

TMP125 125 ml
EUR 96

TMB Precipitating Reagent (For Membranes)

TMP500 500 ml
EUR 159.6

TMB Precipitating Reagent (For Membranes)

TMP999 1000 ml
EUR 236.4

TMB Precipitating (Standard Sensitivity)

TM3125 125 ml
EUR 96

TMB Precipitating (Standard Sensitivity)

TM3500 500 ml
EUR 156

TMB Precipitating (Standard Sensitivity)

TM3999 1000 ml
EUR 235.2

Universal Protein Precipitating Reagent

BSP012 100preps
EUR 97.58

ExoQuick ULTRA EV Isolation Kit for Serum and Plasma

EQULTRA-20A-1 20 reactions
EUR 941

ExoQuick-TC ULTRA EV Isolation Kit for Tissue Culture Media

EQULTRA-20TC-1 20 reactions
EUR 518

Calcium carbonate, 99%, precipitated, Ph. Eur., BP, USP, FCC grade

GX0868-1 1
EUR 33.1

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Cell Media, Luminometric)

K1210-100 each
EUR 1161.6

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Cell media, Colorimetric)

K1205-100 each
EUR 1161.6

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Urine, Colorimetric)

K1202-100 each
EUR 1161.6

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Serum, Colorimetric)

K1203-100 each
EUR 1161.6

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Plasma, Luminometric)

K1206-100 each
EUR 1161.6

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Urine, Luminometric)

K1207-100 each
EUR 1161.6

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Serum, Luminometric)

K1208-100 each
EUR 1161.6

ExoQuant? Overall Exosome Capture and Quantification ELISA Assay Kit (Human Plasma, Colorimetric)

K1201-100 each
EUR 1161.6

Psoriasis Exosome

P141-PS - Ask for price

Exosome Isolation kit (for serum & plasma -Exosome-RNA-NGS)

P101NGS - Ask for price

Lung Cancer Exosome

P141-LG - Ask for price

Leukemia - ALL Exosome

P141-ALL - Ask for price

Leukemia - AML Exosome

P141-AML - Ask for price

Leukemia - CLL Exosome

P141-CLL - Ask for price

Leukemia - CML Exosome

P141-CML - Ask for price

Liver Cancer Exosome

P141-LV - Ask for price

Morpheus - Precipitant Mix 4, 100mL *SHIPPED ON ICE*

M-MD2-100-84 each
EUR 312
Description: Morpheus - Precipitant Mix 4, 100mL *SHIPPED ON ICE*

Breast Cancer Exosome

P141-BR - Ask for price

Crohn's Disease Exosome

P141-CR - Ask for price

Kidney Cancer Exosome

P141-KN - Ask for price

Psoriasis Exosome RNA

P241-PS - Ask for price

Bladder Cancer Exosome

P141-BL - Ask for price

Thyroid Cancer Exosome

P141-TH - Ask for price

Prostate Cancer Exosome

P141-PR - Ask for price

Wizard Precipitant Synergy - Tubes (Precipitant Synergy Protein Crystallization Screen Kit), 192 formulations, 1.7 mL volumes in tubes

M-R-1009539 192 X 1.7 ml
EUR 2064
Description: Wizard Precipitant Synergy - Tubes (Precipitant Synergy Protein Crystallization Screen Kit), 192 formulations, 1.7 mL volumes in tubes

Colorectal Cancer Exosome

P141-CO - Ask for price

Lung Cancer Exosome RNA

P241-LG - Ask for price

Exosome (Pre-isolated) RNA Extraction kit Note! This kit doesn't contain the conponents for exosome enrichment.

EIRK-01 50 reactions
EUR 3380.4
Description: A RNA Extraction kit that is ideal for total RNA (miRNA+mRNAs) extraction from pre-isolated exosomes.

Diabetes (Type 1) Exosome

P141-DB1 - Ask for price

Diabetes (Type 2) Exosome

P141-DB2 - Ask for price

Normal human exosome RNA

P240 - Ask for price

Leukemia - ALL Exosome RNA

P241-ALL - Ask for price

Leukemia - AML Exosome RNA

P241-AML - Ask for price

Leukemia - CLL Exosome RNA

P241-CLL - Ask for price

Leukemia - CML Exosome RNA

P241-CML - Ask for price

Liver Cancer Exosome RNA

P241-LV - Ask for price

Exosome-TEM-easy Kit

P130 - Ask for price

Exo-APC Exosome FACS stain

EXOFLOW810A-1 20 reactions
EUR 188

EXOCET Exosome Quantitation Kit

EXOCET96A-1 96 reactions
EUR 462

Breast Cancer Exosome RNA

P241-BR - Ask for price

Crohn's Disease Exosome RNA

P241-CR - Ask for price

Kidney Cancer Exosome RNA

P241-KN - Ask for price

Exo-FITC Exosome FACS stain

EXOFLOW800A-1 20 reactions
EUR 188

ExoQuant? Tumor-derived Exosome enrichment and Quantification Assay Kit (Biological Fluids/Cell Media, Colorimetric)

K1204-100 each
EUR 1161.6

ExoQuant? Tumor-derived Exosome enrichment and Quantification Assay Kit (Biological Fluids/Cell Media, Luminometric)

K1209-100 each
EUR 1161.6

Rheumatoid Arthritis Exosome

P141-RA - Ask for price

Bladder Cancer Exosome RNA

P241-BL - Ask for price

Thyroid Cancer Exosome RNA

P241-TH - Ask for price

Lyophilized Exosome Standards

ExoA375 100 μg
EUR 364.2

Lyophilized Exosome Standards

ExoHT29 100 μg
EUR 364.2

Lyophilized Exosome Standards

ExoMCF7 100 μg
EUR 364.2

Lyophilized Exosome Standards

ExoPC3 100 μg
EUR 364.2

Lyophilized Exosome Standards

ExoPLASMA 100 μg
EUR 364.2

Lyophilized Exosome Standards

ExoUrine 100 μg
EUR 364.2

Nasopharyngeal Cancer Exosome

P141-NA - Ask for price

Leukemia - Hairy Cell Exosome

P141-HC - Ask for price

Prostate Cancer Exosome RNA

P241-PR - Ask for price

Exosome Component 5 Protein

20-abx260873
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 25 ug
  • 5 ug

Exosome Component 7 Protein

20-abx261347
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

Exosome Component 10 Protein

20-abx261805
  • EUR 5238.00
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

Exosome Component 4 Protein

20-abx261954
  • EUR 5388.00
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

Exosome Component 9 Protein

20-abx262123
  • EUR 393.60
  • EUR 6106.80
  • EUR 276.00
  • 10 ug
  • 1 mg
  • 2 µg

Exosome Component 8 Protein

20-abx262850
  • EUR 393.60
  • EUR 7676.40
  • EUR 276.00
  • 10 ug
  • 1 mg
  • 2 µg

Exosome Component 3 Protein

20-abx263422
  • EUR 276.00
  • EUR 1930.80
  • EUR 393.60
  • 1 µg
  • 50 ug
  • 5 ug

Exosome Component 1 Protein

20-abx263423
  • EUR 1930.80
  • EUR 393.60
  • EUR 276.00
  • 100 ug
  • 10 ug
  • 2 µg

Exosome Component 9 Peptide

46-967P 0.1 mg
EUR 405.6
Description: (CT) PMSCL1 Peptide

Melanoma (skin cancer) Exosome

P141-MN - Ask for price

Uterine Corpus Cancer Exosome

P141-UT - Ask for price

Colorectal Cancer Exosome RNA

P241-CO - Ask for price

Exosome Component 3 (EXOSC3) Antibody

20-abx312069
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Exosome Component 8 (EXOSC8) Antibody

20-abx318649
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Exosome Component 1 (EXOSC1) Antibody

abx232898-100ug 100 ug
EUR 577.2

Exosome Component 2 (EXOSC2) Antibody

abx232901-100ug 100 ug
EUR 577.2

Exosome Component 2 (EXOSC2) Antibody

abx232902-100ug 100 ug
EUR 661.2

Exosome Component 3 (EXOSC3) Antibody

abx232903-100ug 100 ug
EUR 577.2

Exosome Component 4 (EXOSC4) Antibody

abx232904-100ug 100 ug
EUR 577.2

Exosome Component 5 (EXOSC5) Antibody

abx232905-100ug 100 ug
EUR 610.8

Exosome Component 7 (EXOSC7) Antibody

abx232906-100ug 100 ug
EUR 661.2

Exosome Component 8 (EXOSC8) Antibody

abx232907-100ug 100 ug
EUR 577.2

Exosome Component 9 (EXOSC9) Antibody

abx232908-100ug 100 ug
EUR 661.2

Exosome Component 2 (EXOSC2) Antibody

20-abx176346
  • EUR 510.00
  • EUR 159.60
  • EUR 1446.00
  • EUR 693.60
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Exosome Component 2 (EXOSC2) Antibody

20-abx172308
  • EUR 1028.40
  • EUR 526.80
  • 1 mg
  • 200 ug

Exosome Component 1 (EXOSC1) Antibody

20-abx112409
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Exosome Component 2 (EXOSC2) Antibody

20-abx112411
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Exosome Component 3 (EXOSC3) Antibody

20-abx112412
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Exosome Component 4 (EXOSC4) Antibody

20-abx112413
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Exosome Component 5 (EXOSC5) Antibody

20-abx112414
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Exosome Component 8 (EXOSC8) Antibody

20-abx112415
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Exosome Component 1 (EXOSC1) Antibody

abx030303-400ul 400 ul
EUR 627.6

Exosome Component 1 (EXOSC1) Antibody

abx030303-80l 80 µl
EUR 343.2

Exosome Component 8 (EXOSC8) Antibody

abx031706-400ul 400 ul
EUR 627.6

Exosome Component 8 (EXOSC8) Antibody

abx031706-80l 80 µl
EUR 343.2

Exosome Component 2 (EXOSC2) Antibody

abx034983-400ul 400 ul
EUR 627.6

Exosome Component 2 (EXOSC2) Antibody

abx034983-80l 80 µl
EUR 343.2

Exosome Component 4 (EXOSC4) Antibody

abx029390-400ul 400 ul
EUR 627.6

Exosome Component 4 (EXOSC4) Antibody

abx029390-80l 80 µl
EUR 343.2

Exosome Component 7 (EXOSC7) Antibody

20-abx124556
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Exosome Component 4 (EXOSC4) Antibody

20-abx124664
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Exosome Component 1 (EXOSC1) Antibody

20-abx125829
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Exosome Component 2 (EXOSC2) Antibody

20-abx125830
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Exosome Component 3 (EXOSC3) Antibody

20-abx125831
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Exosome Component 8 (EXOSC8) Antibody

20-abx003377
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Exosome Component 2 (EXOSC2) Antibody

20-abx003412
  • EUR 493.20
  • EUR 710.40
  • 100 ul
  • 200 ul

Exosome Component 1 (EXOSC1) Antibody

20-abx301763
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Exosome Component 5 (EXOSC5) Antibody

20-abx302857
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Exo-Check Exosome Antibody Array

322-EXO-FBS-50A-1 100 µg
EUR 469.2

Exo-Check Exosome Antibody Array

EXORAY200A-4 4 arrays
EUR 648

Exo-Check Exosome Antibody Array

EXORAY200B-4 4 arrays
EUR 480

Exo-Check Exosome Antibody Array

EXORAY210A-8 8 arrays
EUR 1176

Exo-Check Exosome Antibody Array

EXORAY210B-8 8 arrays
EUR 910

Diabetes (Type 1) Exosome RNA

P241-DB1 - Ask for price

The exosome-loaded doxorubicin (Exo-Dox) is made by mixing exosome with Dox-HCl, desalinizing with triethylamine and then dialyzing against PBS overnight. Nanoparticle tracking analysis (NTA) and transmission electron microscopy (TEM) was used to characterize of exosome and Exo-Dox.