Urine miR-21-5p and miR-200c-3p as potential non-invasive biomarkers in patients with prostate cancer.

To evaluate miR-21-5p and miR-200C-3p expression in the urine of patients with prostate cancer (PCa) and to investigate their potential as biomarkers.The urine samples collected from 80 patients, including 20 patients who were diagnosed with benign prostatic hyperplasia ( BPH) and 60 patients diagnosed with PCa, were examined. The exosome isolation is done by using miRCURY exosome isolation kit (Exiqon, Denmark), total RNA was extracted using the miRCURY RNA Isolation Kit-Biofluid kit (Exiqon, Denmark), and complementary DNA (cDNA) was synthesized using a kit Universal cDNA Synthesis (Exiqon, Denmark ).

A quantitative polymerase chain reaction (qPCR) analysis of gene expression was performed using qPCR CFX 96 thermocycler (Bio-Rad). All procedures followed by manufacturers recommendations.The overexpressions of miR-21 in a group of non-metastatic PCa and metastatic PCa compared with BPH group was statistically significant with p-values ​​of 0.001 and 0.018, respectively. The non-metastatic PCa compared with metastatic PCa group was also statistically significant with a p-value of 0.037. Under the expression of miR-200C in non-metastatic PCa and PCa metastasis group compared to the BPH group was statistically significant with p-values ​​of 0.001 and 0.001, respectively.

The overexpressions of miR-21 was found in this study could be a potential non-invasive diagnostic tool for patients with PCa. Despite the significant results in our study, the use of micro-RNA in urine samples may vary due to epigenetic variation. Further studies with a larger population is needed to investigate the role of miR-21 and miR-200C as biomarkers in PCa.

 Urine miR-21-5p and miR-200c-3p as potential non-invasive biomarkers in patients with prostate cancer.
Urine miR-21-5p and miR-200c-3p as potential non-invasive biomarkers in patients with prostate cancer.

Circulating microRNAs in extracellular vesicles as a potential biomarker for psoriatic arthritis in patients with psoriasis.

Psoriatic arthritis (PsA) develops in ~ 30% of patients with psoriasis. The diagnosis of PsA challenging and there is no reliable molecular markers in clinical use. MicroRNAs are short noncoding regulatory RNAs, which can be actively packaged in extracellular vesicles (EV) and secreted into circulation.To investigate whether plasma-derived microRNAs EV can serve as a biomarker for PsA in patients with psoriasis.


METHOD
plasma samples obtained from patients with psoriasis the skin only (PSC) and patients with psoriasis and PsA. EVS plasma was isolated using Exosome miRCURYTM Isolation Kit. RNA sequencing was used to identify differentially expressed miRNAs EV in discovery phase (PSC, n = 15; PsA, n = 14). In the validation phase (PSC, n = 29; PsA, n = 28), 41 miRNAs been analyzed in plasma EVs by qPCR. Association of miRNAs identified with PsA assessed by logistic regression analysis.


RNA sequencing identified 19 miRNAs EV plasma levels differed significantly between PsA and PSC in the category of inventions. significantly lower levels of let-7b plasma EV-5p and miR-30e-5p in PsA vs. PSC confirmed in the validation cohort, and decreased their levels found to be associated with the presence of PsA. ROC analysis revealed AUC of 0.68 (95% CI 0.53 to 0.83) for the let-7b-5p and 0.69 (95% CI from 0.55 to 0.84) to the level of miR-30e-5p.Circulating EV microRNA altered in patients with PsA compared with the PSC. The findings of this exploratory study shows that circulating microRNAs EV can serve as a biomarker for arthritis in patients with psoriasis.


The main objective of this research is to develop nano-drug consisting of doxorubicin and exosome derived from mesenchymal stem cells, and to explore its effect on osteosarcoma in vitro.The exosomes isolated from bone marrow MSCs (BM-MSC) by Exosome Isolation Kit.

PEG-it Virus Precipitation Solution (250 mL aliquot)

LV825A-1 250 ml
EUR 711

TMB for Precipitation

42R-TB100x 1000 ml
EUR 473
Description: High sensitivity TMB Substrate for Precipitation assays

TMB for Precipitation

MBS538888-1L 1L
EUR 620

TMB for Precipitation

MBS538888-5x1L 5x1L
EUR 2700

DNA Precipitation Buffer

D106 55 ml
EUR 210
Description: DNA Precipitation Buffer by Cygnus Technologies is available in Europe via Gentaur.

ExoQuick Exosome RNA Column Purification kit

EQ808A-1 20 preps
EUR 344

PEG Virus Precipitation Kit

K2190-200 200 preparations
EUR 1270.8

PEG Virus Precipitation Kit

K2190-50 50 preparations
EUR 582.4
Description: Kits|Tools Kit#Tools Kit|Virus Concentration Kit#Kits

PEG Virus Precipitation Kit

K904-200 each
EUR 1201.2

PEG Virus Precipitation Kit

K904-50 each
EUR 451.2

Lentivirus Precipitation Reagent

VC125 250 ml
EUR 432.6

Lentivirus Precipitation Reagent

VC150 500 ml
EUR 814.8

Retrovirus Precipitation Reagent

VC200 100 ml
EUR 194.6

Retrovirus Precipitation Reagent

VC225 250 ml
EUR 432.6

Retrovirus Precipitation Reagent

VC250 500 ml
EUR 814.8

Lentivirus Precipitation Reagent

VC100 100 ml
EUR 195.3

ExoQuick-LP for lipoprotein pre-clear & exosome isolation

EXOLP5A-1 5 reactions
EUR 473

Chicken IgY precipitation reagent

85R-1010 4 ml
EUR 638
Description: Chicken IgY precipitation reagent for use in immunoassays

Chicken IgY precipitation reagent

MBS5307794-2mL 2mL
EUR 975

Chicken IgY precipitation reagent

MBS5307794-5x2mL 5x2mL
EUR 4245

In Vivo SDS-K+ Precipitation Kit

TG1011-1 100 assays
EUR 362.4

In Vivo SDS-K+ Precipitation Kit

TG1011-2 250 assays
EUR 496.8

Nucleic Acid Precipitation Enhancer

TBS6010 2 x0.5 mL
EUR 98

In Vitro SDS-K+ Precipitation Kit

TG1010-1 100 assays
EUR 550.8

In Vitro SDS-K+ Precipitation Kit

TG1010-2 250 assays
EUR 685.2

50ML Protein Precipitation Kit - 50ML

NAT1254 50ML
EUR 348.3

PEG Virus Precipitation Kit - 200 preparations

GWB-AXR384 200 preparations Ask for price

PEG Virus Precipitation Kit - 50 preparations

GWB-AXR385 50 preparations Ask for price

101Bio High Efficiency Protein Precipitation kit

P5W6 30 ml / 30 ml
EUR 129

Kinesis TELOS PPT Protein Precipitation Plate - EACH

CHR6509 EACH
EUR 118.97

LPA (Linear Polyacrylamide) for DNA/RNA Precipitation

10510000-1 10 mg
EUR 105.44

LPA (Linear Polyacrylamide) for DNA/RNA Precipitation

10510000-2 25 mg
EUR 172.85

Minute TM High Efficiency Protein Precipitation kit

WA-006 each
EUR 160

Kinesis TELOS PPT Protein Precipitation Columns - PK100

CHR1378 PK100
EUR 249.75

ExoQuick-CG cGMP-grade Exosome Isolation reagent (50 ml)

EXOCG50A-1 50 ml
EUR 1106

ExoQuick PLUS Exosome Purification Kit - for serum and plasma

EQPL10A-1 10 reactions
EUR 518

ExoQuick Exosome Isolation and RNA Purification kit (for Serum and Plasma)

EQ806A-1 20 preps
EUR 493

ExoQuick Exosome Isolation and RNA Purification kit (for Tissue Culture Media)

EQ806TC-1 10 preps
EUR 493

MinuteTM Native Protein Precipitation Kit from Saliva (20 tests)

SV-043 each
EUR 330

ExoQuick-TC PLUS Exosome Purification Kit - for tissue culture media

EQPL10TC-1 10 reactions
EUR 518

SeraMir Exosome RNA Purification kit (5ml ExoQuick and 20 exoRNA columns)

RA806A-1 20 preps
EUR 493

SeraMir Exosome RNA Purification kit (10ml ExoQuick-TC and 10 exoRNA columns) 10 preps

RA806TC-1 10 preps
EUR 493

SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns)

RA800TC-1 10 reactions
EUR 595

Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome)

P101EZ - Ask for price

Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome)

P100EZ - Ask for price

Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome) sample

P101EZ--sample - Ask for price

ExoQuick TC 10ml

EXOTC10A-1 10 ml
EUR 341

ExoQuick TC 50ml

EXOTC50A-1 50 ml
EUR 1147

Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome) sample

P100EZ-sample - Ask for price

Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800TC-1 with 50ml ExoQuick-TC, RA805A-1 and RA812A-1 components)

RA822TC-1 20 profiles
EUR 1533

TMB Precipitating (High Sensitivity)

TM5125 125 ml
EUR 28.28

TMB Precipitating (High Sensitivity)

TM5500 500 ml
EUR 70.71

TMB Precipitating (High Sensitivity)

TM5999 1000 ml
EUR 127.28

TMB Precipitating Reagent (For Membranes)

TMP125 125 ml
EUR 96

TMB Precipitating Reagent (For Membranes)

TMP500 500 ml
EUR 159.6

TMB Precipitating Reagent (For Membranes)

TMP999 1000 ml
EUR 236.4

TMB Precipitating (Standard Sensitivity)

TM3125 125 ml
EUR 24.43

TMB Precipitating (Standard Sensitivity)

TM3500 500 ml
EUR 64.28

TMB Precipitating (Standard Sensitivity)

TM3999 1000 ml
EUR 117

ExoQuick ULTRA EV Isolation Kit for Serum and Plasma

EQULTRA-20A-1 20 reactions
EUR 941

Universal Protein Precipitating Reagent

BSP012 100preps
EUR 97.58

ExoQuick-TC ULTRA EV Isolation Kit for Tissue Culture Media

EQULTRA-20TC-1 20 reactions
EUR 518

ExoQualiTM Exosome DNA Extraction Kit (Pre-Isolated Exosomes)

CEIK-050L-30T 30T Ask for price

ExoQualiTM Exosome DNA Extraction Kit (Pre-Isolated Exosomes)

CEIK-050L-50T 50T Ask for price

ExoQualiTM Exosome RNA Extraction Kit (Pre-Isolated Exosomes)

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Sulfur Precipitated pure, 98%

69181 500 Gms
EUR 1.73
Description: Part B

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GRM622-500G 1 unit
EUR 13.55
Description: Silicic acid, precipitated

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RM1316-500G 1 unit
EUR 11.59
Description: Sulphur precipitated, Hi-AR™

Calcium resinate (precipitated)

C01800 25G
EUR 113.58
Description: CAS N° 9007-13-0

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CEIK-003L-10T 10 T Ask for price

ExoQualiTM Exosome Isolation Kit (Urine)

CEIK-003L-30T 30 T Ask for price

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ESEC-001L-5columns 5 columns Ask for price

Magnesium stearate precipitated fine pow

GRM7219-500G 1 unit
EUR 6.93
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Rabbit plasma, barium chloride precipitate

MBS136153-500mL 500mL
EUR 1360

Rabbit plasma, barium chloride precipitate

MBS136153-50mL 50mL
EUR 270

Rabbit plasma, barium chloride precipitate

MBS136153-5x500mL 5x500mL
EUR 5930

Calcium carbonate, 99%, precipitated, Ph. Eur., BP, USP, FCC grade

GX0868 1kg
EUR 30.35

Calcium carbonate, 99%, precipitated, Ph. Eur., BP, USP, FCC grade

GX0868-1 1
EUR 33.1

ExoQualiTM Exosome Isolation Kit (Cell Media)

CEIK-001L-10T 10 T Ask for price

ExoQualiTM Exosome Isolation Kit (Cell Media)

CEIK-001L-30T 30 T Ask for price

ExoQualiTM Exosome Isolation Kit (All in one)

CEIK-008L-10T 10 T Ask for price

ExoQualiTM Exosome Isolation Kit (All in one)

CEIK-008L-30T 30 T Ask for price

ExoQualiTM Max Exosome Chromatography Columns

ESEC-002L-3columns 3 columns Ask for price

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CEIK-006L-10T 10 T Ask for price

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ExoQualiTM Exosome Isolation Kit (Stem Cell Media)

CEIK-002L-10T 10 T Ask for price

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CEIK-002L-30T 30 T Ask for price

ExoQualiTM Exosome Isolation Kit (other body fluids)

CEIK-007L-10T 10 T Ask for price

ExoQualiTM Exosome Isolation Kit (other body fluids)

CEIK-007L-30T 30 T Ask for price

ExoQualiTM Exosome DNA Extraction Kit (Plasma & Serum)

CEIK-048L-30T 30T Ask for price

ExoQualiTM Exosome DNA Extraction Kit (Plasma & Serum)

CEIK-048L-50T 50T Ask for price

ExoQualiTM Exosome RNA Extraction Kit (Plasma & Serum)

CEIK-093L-30T 30T Ask for price

ExoQualiTM Exosome RNA Extraction Kit (Plasma & Serum)

CEIK-093L-50T 50T Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Serum )

CEIK-070L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Serum )

CEIK-070L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Urine )

CEIK-071L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Urine )

CEIK-071L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Plasma )

CEIK-068L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Plasma )

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ExoQuali™ Lyophilized Exosome Standards ( Human Saliva )

CEIK-069L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

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CEIK-069L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from serum)

CEIK-044L-30T 30 T Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from serum)

CEIK-044L-50T 50 T Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from urine)

CEIK-046L-10T 10 T Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from urine)

CEIK-046L-24T 24 T Ask for price

ExoQuali™ Lyophilized Exosome Standards ( MM1 cell line )

CEIK-064L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

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CEIK-064L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( PC3 cell line )

CEIK-065L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( PC3 cell line )

CEIK-065L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from plasma)

CEIK-045L-30T 30 T Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from plasma)

CEIK-045L-50T 50 T Ask for price

ExoQuali™ Lyophilized Exosome Standards ( A549 cell line )

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ExoQuali™ Lyophilized Exosome Standards ( A549 cell line )

CEIK-054L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQualiTM Total Exosome Capture & Quantification Kit (Serum)

CEIK-107L-96wells 96 wells Ask for price

ExoQualiTM Total Exosome Capture & Quantification Kit (Urine)

CEIK-110L-96wells 96 wells Ask for price

ExoQuali™ Lyophilized Exosome Standards ( COLO1 cell line )

CEIK-058L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( COLO1 cell line )

CEIK-058L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQualiTM Total Exosome Capture & Quantification Kit (Plasma)

CEIK-108L-96wells 96 wells Ask for price

ExoQuali™ Lyophilized Exosome Standards ( U87 MG cell line )

CEIK-067L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

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CEIK-067L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( B16F10 cell line )

CEIK-055L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( B16F10 cell line )

CEIK-055L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( BLCL21 cell line )

CEIK-056L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

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ExoQuali™ Lyophilized Exosome Standards ( HCT116 cell line )

CEIK-060L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

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ExoQuali™ Lyophilized Exosome Standards ( K-562 cell line )

CEIK-062L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( K-562 cell line )

CEIK-062L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQualiTM Overall Exosome Isolation Immunoplates (from Serum)

CEIK-023L-96wells 96 wells Ask for price

ExoQualiTM Overall Exosome Isolation Immunoplates (from Saliva)

CEIK-026L-96wells 96 wells Ask for price

ExoQualiTM Overall Exosome Isolation Immunoplates (from Plasma)

CEIK-095L-96wells 96 wells Ask for price

ExoQualiTM Overall Exosome Isolation Immunoplates (from Urine)

CEIK-096L-96wells 96 wells Ask for price

ExoQualiTM Exosome DNA Extraction Kit (Urine & Cell Supernatant)

CEIK-049L-10T 10T Ask for price

ExoQualiTM Exosome DNA Extraction Kit (Urine & Cell Supernatant)

CEIK-049L-20T 20T Ask for price

ExoQualiTM Exosome RNA Extraction Kit (Urine & Cell Supernatant)

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ExoQualiTM Exosome RNA Extraction Kit (Urine & Cell Supernatant)

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ExoQuali™ Lyophilized Exosome Standards ( SK-N-SH cell line )

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*Innovative Grade US Origin Rabbit Barium Chloride Precipitate Plasma Lyophilized

IRBBACL2PPLALY500ML each
EUR 1045
Description: *Innovative Grade US Origin Rabbit Barium Chloride Precipitate Plasma Lyophilized

*Innovative Grade US Origin Rabbit Barium Chloride Precipitate Plasma Lyophilized

IRBBACL2PPLALY50ML each
EUR 150
Description: *Innovative Grade US Origin Rabbit Barium Chloride Precipitate Plasma Lyophilized

ExoQualiTM Overall Exosome Isolation Immunoplates (from Cell Media)

CEIK-029L-96wells 96 wells Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from other body fluids)

CEIK-047L-10T 10 T Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from other body fluids)

CEIK-047L-24T 24 T Ask for price

ExoQualiTM Total Exosome Capture & Quantification Kit (Cell Supernatant)

CEIK-109L-96wells 96 wells Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from cell culture media)

CEIK-043L-10T 10 T Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from cell culture media)

CEIK-043L-24T 24 T Ask for price

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Cell media, Colorimetric)

K1205-100 each
EUR 1161.6

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Cell Media, Luminometric)

K1210-100 each
EUR 1161.6

ExoQualiTM Human Exosome Capture and Isolation Kits (for Serum/Plasma) (CD9 Capture Beads)

CEIK-101L-10T 10T Ask for price

The exosome-loaded doxorubicin (Exo-Dox) is made by mixing exosome with Dox-HCl, desalinizing with triethylamine and then dialyzing against PBS overnight. Nanoparticle tracking analysis (NTA) and transmission electron microscopy (TEM) was used to characterize of exosome and Exo-Dox.