Urine miR-21-5p and miR-200c-3p as potential non-invasive biomarkers in patients with prostate cancer.

To evaluate miR-21-5p and miR-200C-3p expression in the urine of patients with prostate cancer (PCa) and to investigate their potential as biomarkers.The urine samples collected from 80 patients, including 20 patients who were diagnosed with benign prostatic hyperplasia ( BPH) and 60 patients diagnosed with PCa, were examined. The exosome isolation is done by using miRCURY exosome isolation kit (Exiqon, Denmark), total RNA was extracted using the miRCURY RNA Isolation Kit-Biofluid kit (Exiqon, Denmark), and complementary DNA (cDNA) was synthesized using a kit Universal cDNA Synthesis (Exiqon, Denmark ).

A quantitative polymerase chain reaction (qPCR) analysis of gene expression was performed using qPCR CFX 96 thermocycler (Bio-Rad). All procedures followed by manufacturers recommendations.The overexpressions of miR-21 in a group of non-metastatic PCa and metastatic PCa compared with BPH group was statistically significant with p-values ​​of 0.001 and 0.018, respectively. The non-metastatic PCa compared with metastatic PCa group was also statistically significant with a p-value of 0.037. Under the expression of miR-200C in non-metastatic PCa and PCa metastasis group compared to the BPH group was statistically significant with p-values ​​of 0.001 and 0.001, respectively.

The overexpressions of miR-21 was found in this study could be a potential non-invasive diagnostic tool for patients with PCa. Despite the significant results in our study, the use of micro-RNA in urine samples may vary due to epigenetic variation. Further studies with a larger population is needed to investigate the role of miR-21 and miR-200C as biomarkers in PCa.

 Urine miR-21-5p and miR-200c-3p as potential non-invasive biomarkers in patients with prostate cancer.
Urine miR-21-5p and miR-200c-3p as potential non-invasive biomarkers in patients with prostate cancer.

Circulating microRNAs in extracellular vesicles as a potential biomarker for psoriatic arthritis in patients with psoriasis.

Psoriatic arthritis (PsA) develops in ~ 30% of patients with psoriasis. The diagnosis of PsA challenging and there is no reliable molecular markers in clinical use. MicroRNAs are short noncoding regulatory RNAs, which can be actively packaged in extracellular vesicles (EV) and secreted into circulation.To investigate whether plasma-derived microRNAs EV can serve as a biomarker for PsA in patients with psoriasis.


METHOD
plasma samples obtained from patients with psoriasis the skin only (PSC) and patients with psoriasis and PsA. EVS plasma was isolated using Exosome miRCURYTM Isolation Kit. RNA sequencing was used to identify differentially expressed miRNAs EV in discovery phase (PSC, n = 15; PsA, n = 14). In the validation phase (PSC, n = 29; PsA, n = 28), 41 miRNAs been analyzed in plasma EVs by qPCR. Association of miRNAs identified with PsA assessed by logistic regression analysis.


RNA sequencing identified 19 miRNAs EV plasma levels differed significantly between PsA and PSC in the category of inventions. significantly lower levels of let-7b plasma EV-5p and miR-30e-5p in PsA vs. PSC confirmed in the validation cohort, and decreased their levels found to be associated with the presence of PsA. ROC analysis revealed AUC of 0.68 (95% CI 0.53 to 0.83) for the let-7b-5p and 0.69 (95% CI from 0.55 to 0.84) to the level of miR-30e-5p.Circulating EV microRNA altered in patients with PsA compared with the PSC. The findings of this exploratory study shows that circulating microRNAs EV can serve as a biomarker for arthritis in patients with psoriasis.


The main objective of this research is to develop nano-drug consisting of doxorubicin and exosome derived from mesenchymal stem cells, and to explore its effect on osteosarcoma in vitro.The exosomes isolated from bone marrow MSCs (BM-MSC) by Exosome Isolation Kit.

PEG-it Virus Precipitation Solution (100 mL aliquot)

LV810A-1 100 ml
EUR 382

PEG-it Virus Precipitation Solution (250 mL aliquot)

LV825A-1 250 ml
EUR 777

ExoQuick PLUS Exosome Purification Kit - for serum and plasma

EQPL10A-1 10 reactions
EUR 579

ExoQuick-CG cGMP-grade Exosome Isolation reagent (50 ml)

EXOCG50A-1 50 ml
EUR 1181

ExoQuick-TC PLUS Exosome Purification Kit - for tissue culture media

EQPL10TC-1 10 reactions
EUR 579

Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL)

TMEXO-1 100 reactions
EUR 583

ExoQuick Exosome Isolation and RNA Purification kit (for Serum and Plasma)

EQ806A-1 20 preps
EUR 554

ExoQuick Exosome Isolation and RNA Purification kit (for Tissue Culture Media)

EQ806TC-1 10 preps
EUR 554

SeraMir Exosome RNA Purification kit (5ml ExoQuick and 20 exoRNA columns)

RA806A-1 20 preps
EUR 554

Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome)

P101EZ - Ask for price

DNA Precipitation Buffer

D106 55 ml
EUR 175
Description: DNA Precipitation Buffer by Cygnus Technologies is available in Europe via Gentaur.

TMB for Precipitation

42R-TB100x 1000 ml
EUR 538
Description: High sensitivity TMB Substrate for Precipitation assays

Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome)

P100EZ - Ask for price

Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome) sample

P101EZ--sample - Ask for price

ExoQuick TC 10ml

EXOTC10A-1 10 ml
EUR 399

ExoQuick TC 50ml

EXOTC50A-1 50 ml
EUR 1222

Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome) sample

P100EZ-sample - Ask for price

50ML Protein Precipitation Kit

NAT1254 50ML
EUR 225

PEG Virus Precipitation Kit

K2190-200 200 preparations
EUR 1059

PEG Virus Precipitation Kit

K2190-50 50 preparations
EUR 405

Chicken IgY precipitation reagent

85R-1010 4 ml
EUR 554
Description: Chicken IgY precipitation reagent for use in immunoassays

PEG Virus Precipitation Kit

K904-200
EUR 1001

PEG Virus Precipitation Kit

K904-50
EUR 376

SeraMir Exosome RNA Purification kit (10ml ExoQuick-TC and 10 exoRNA columns) 10 preps

RA806TC-1 10 preps
EUR 554

SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns)

RA800TC-1 10 reactions
EUR 659

101Bio High Efficiency Protein Precipitation kit

P5W6 - Ask for price

In Vitro SDS-K+ Precipitation Kit

TG1010-1 100 assays
EUR 459

In Vitro SDS-K+ Precipitation Kit

TG1010-2 250 assays
EUR 571

In Vivo SDS-K+ Precipitation Kit

TG1011-1 100 assays
EUR 302

In Vivo SDS-K+ Precipitation Kit

TG1011-2 250 assays
EUR 414

Psoriasis Exosome

P141-PS - Ask for price

Sodium Pyruvate Solution, 100 mM Solution

CCM1753-100 100 mL
EUR 60.84

Stop Solution

F006 12 ml
EUR 180
Description: Stop Solution by Cygnus Technologies is available in Europe via Gentaur.

DTT Solution

R-1104 1 ml, 500 mM
EUR 109.45
Description: fast delivery possible

Denaturing solution

DE540 1L
EUR 61.31

AlphaBioCoat Solution

AC001 20 ml
EUR 271

TRIMMING SOLUTION

99-676-CM 1 L /pk
EUR 88
Description: Specialty Media; Islet products

Schiff's Solution

SRF030 30 ml
EUR 64

Schiff's Solution

SRF250 250 ml
EUR 77

Schiff's Solution

SRF3800 1 Gal.
EUR 345

Schiff's Solution

SRF500 500 ml
EUR 103

Schiff's Solution

SRF999 1000 ml
EUR 149

Tartrazine Solution

TZQ125 125 ml
EUR 64

Tartrazine Solution

TZQ500 500 ml
EUR 98

Tartrazine Solution

TZQ999 1000 ml
EUR 127

Orcein Solution

OAA125 125 ml
EUR 66

Orcein Solution

OAA500 500 ml
EUR 109

Orcein Solution

OAA999 1000 ml
EUR 148

Methenamine Solution

MET125 125 ml
EUR 64

Methenamine Solution

MET500 500 ml
EUR 95

Methenamine Solution

MET999 1000 ml
EUR 132

Borax Solution

BOR015 15 ml
EUR 56

Borax Solution

BOR125 125 ml
EUR 79

Borax Solution

BOR500 500 ml
EUR 119

Borax Solution

BOR999 1000 ml
EUR 183

Prehybridization Solution

K2191050-1 5 ml
EUR 137
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Hybridization Solution

K2191050-2 5 ml
EUR 157
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Blocking Solution

K2191050-8 250 ul
EUR 137
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Plug Solution

GR103038 500 mL
EUR 85

SDS Solution

2102-100
EUR 137

EDTA Solution

2103-100
EUR 137

Leukemia - ALL Exosome

P141-ALL - Ask for price

Leukemia - AML Exosome

P141-AML - Ask for price

Bladder Cancer Exosome

P141-BL - Ask for price

Breast Cancer Exosome

P141-BR - Ask for price

Leukemia - CLL Exosome

P141-CLL - Ask for price

Leukemia - CML Exosome

P141-CML - Ask for price

Colorectal Cancer Exosome

P141-CO - Ask for price

Crohn's Disease Exosome

P141-CR - Ask for price

Kidney Cancer Exosome

P141-KN - Ask for price

Lung Cancer Exosome

P141-LG - Ask for price

Liver Cancer Exosome

P141-LV - Ask for price

Nasopharyngeal Cancer Exosome

P141-NA - Ask for price

Prostate Cancer Exosome

P141-PR - Ask for price

Rheumatoid Arthritis Exosome

P141-RA - Ask for price

Thyroid Cancer Exosome

P141-TH - Ask for price

Psoriasis Exosome RNA

P241-PS - Ask for price

Lyophilized Exosome Standards

ExoA375 100 μg
EUR 303.5

Lyophilized Exosome Standards

ExoHT29 100 μg
EUR 303.5

Lyophilized Exosome Standards

ExoMCF7 100 μg
EUR 303.5

Lyophilized Exosome Standards

ExoPC3 100 μg
EUR 303.5

Lyophilized Exosome Standards

ExoPLASMA 100 μg
EUR 303.5

Lyophilized Exosome Standards

ExoUrine 100 μg
EUR 303.5

Gentamicin Solution, Gentamicin Solution, 50 mg/mL

CCM1123-010 10 mL
EUR 64.05

Exosome Isolation kit (for serum & plasma -Exosome-RNA-NGS)

P101NGS - Ask for price

Bovine Anti-Chicken IgY Beads for immuno-precipitation

XG-6134 1 mL
EUR 455.6
Description: The anti-IgY beads were made by cross-linking of bovine IgG anti-IgY antibodies to Protein A/G agarose beads. IgY is the original designation of chicken IgG like immunoglobulin.

Fluorescent Exosome Standard (Urine )

ESF-15 100 µg
EUR 1723
Description: Fluorescent exosomes are labeled with green dye, which offer a stable fluorescent labeling.

Exosome-TEM-easy Kit

P130 - Ask for price

Normal human exosome sample

P140 - Ask for price

Diabetes (Type 1) Exosome

P141-DB1 - Ask for price

Diabetes (Type 2) Exosome

P141-DB2 - Ask for price

Leukemia - Hairy Cell Exosome

P141-HC - Ask for price

Leukemia - Multiple Myeloma Exosome

P141-MM - Ask for price

Melanoma (skin cancer) Exosome

P141-MN - Ask for price

Non-Hodgkin lymphoma Exosome

P141-NH - Ask for price

Systemic Lupus Erythematosus Exosome

P141-SL - Ask for price

Uterine Cervical Cancer Exosome

P141-UC - Ask for price

Uterine Corpus Cancer Exosome

P141-UT - Ask for price

Normal human exosome RNA

P240 - Ask for price

Leukemia - ALL Exosome RNA

P241-ALL - Ask for price

Leukemia - AML Exosome RNA

P241-AML - Ask for price

Bladder Cancer Exosome RNA

P241-BL - Ask for price

Breast Cancer Exosome RNA

P241-BR - Ask for price

Leukemia - CLL Exosome RNA

P241-CLL - Ask for price

Leukemia - CML Exosome RNA

P241-CML - Ask for price

Colorectal Cancer Exosome RNA

P241-CO - Ask for price

Crohn's Disease Exosome RNA

P241-CR - Ask for price

Kidney Cancer Exosome RNA

P241-KN - Ask for price

Lung Cancer Exosome RNA

P241-LG - Ask for price

Liver Cancer Exosome RNA

P241-LV - Ask for price

Nasopharyngeal Cancer Exosome RNA

P241-NA - Ask for price

Prostate Cancer Exosome RNA

P241-PR - Ask for price

Rheumatoid Arthritis Exosome RNA

P241-RA - Ask for price

Thyroid Cancer Exosome RNA

P241-TH - Ask for price

Exosome Component 5 Protein

20-abx260873
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 25 ug
  • 5 ug

Exosome Component 7 Protein

20-abx261347
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug

Exosome Component 10 Protein

20-abx261805
  • EUR 4365.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug

Exosome Component 4 Protein

20-abx261954
  • EUR 4490.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug

Exosome Component 9 Protein

20-abx262123
  • EUR 328.00
  • EUR 5089.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg

Exosome Component 8 Protein

20-abx262850
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg

Exosome Component 3 Protein

20-abx263422
  • EUR 230.00
  • EUR 1609.00
  • EUR 328.00
  • 1 µg
  • 50 ug
  • 5 ug

Exosome Component 1 Protein

20-abx263423
  • EUR 1609.00
  • EUR 328.00
  • EUR 230.00
  • 100 ug
  • 10 ug
  • 2 µg

EXOCET Exosome Quantitation Kit

EXOCET96A-1 96 reactions
EUR 521

FluoroCet Exosome Quantitation Kit

FCET96A-1 96 reactions
EUR 726

Exosome Component 9 Peptide

46-967P 0.1 mg
EUR 338
Description: (CT) PMSCL1 Peptide

Stop Solution, 25ML

X020-25ML 25ML
EUR 109

Stop Solution, 5ML

X020-5ML 5ML
EUR 65

Stop Solution, 1ML

X046-1ML 1ML
EUR 65

Creatinine Solution, 100ML

X116-100ML 100ML
EUR 131

Creatinine Solution, 25ML

X120-25ML 25ML
EUR 173

Extraction Solution, 250ML

X123-250ML 250ML
EUR 263

Extraction Solution, 50ML

X123-50ML 50ML
EUR 123

Mesotocin Solution, 625UL

X127-625UL 625UL
EUR 227

Isotocin Solution, 625UL

X128-625UL 625UL
EUR 227

Stop Solution, 5ML

X134-5ML 5ML
EUR 65

RNA Stabilization Solution

FARSS-001 100ml
EUR 208

Glutaraldehyde, 50% solution

G0875 25ml
EUR 60.44

Glutaraldehyde, 25% solution

GC3870 100ml
EUR 60.44

calibration solution ammonium

ISC20 ea
EUR 91

calibration solution bromide

ISC21 ea
EUR 91

calibration solution calcium

ISC23 ea
EUR 91

calibration solution chloride

ISC24 ea
EUR 91

calibration solution cupric

ISC25 ea
EUR 91

calibration solution fluoride

ISC27 ea
EUR 91

calibr. solution fluoroborate

ISC28 ea
EUR 91

calibration solution iodide

ISC29 ea
EUR 91

calibration solution nitrate

ISC31 ea
EUR 91

cal. solution perchlorate

ISC32 ea
EUR 91

cal. solution potassium

ISC33 ea
EUR 91

calibration solution silver

ISC34 ea
EUR 91

calibration solution sodium

ISC35 ea
EUR 91

cal. solution surfactant

ISC36 ea
EUR 91

calibration solution ammonia

ISC50 ea
EUR 91

Glyoxal, 40% solution

D0238 1Kg
EUR 75.91

1L Denaturation Solution

NAT1232 1L
EUR 85

1L Neutralization Solution

NAT1234 1L
EUR 85

100ML Eosin Solution

NAT1340 100ML
EUR 89

The exosome-loaded doxorubicin (Exo-Dox) is made by mixing exosome with Dox-HCl, desalinizing with triethylamine and then dialyzing against PBS overnight. Nanoparticle tracking analysis (NTA) and transmission electron microscopy (TEM) was used to characterize of exosome and Exo-Dox.