Circular RNAs expression profiles in plasma exosomes from early-stage lung adenocarcinoma and the potential biomarkers.

This study aims to identify the circular RNA differential (circRNA) in exosomes plasma of patients with lung adenocarcinoma (LUAD) using high-throughput sequencing. First, exosomes were isolated using a kit exosome isolation and confirmed by Western blotting, transmission electron microscopy, and NanoSight Assay. Furthermore, the expression profile circRNA plasma is filtered by high-throughput sequencing and confirmed by fluorescence quantitative real-time polymerase chain reaction (qRT-PCR) and sequencing Sanger.

Finally, network-miRNA-mRNA circRNA performed to predict the potential function circRNAs. The results of the high-throughput sequencing data documented that exosomal circRNAs 182 differentially expressed in all screened, including 105 upregulated and 78 were downregulated in plasma LUAD patients compared with controls. Fourth circRNAs regulated including circ_0001492, circ_0001346, circ_0000690, and circ_0001439 synonymous with sequencing data by qRT-PCR, and the latent interaction circRNA-miRNA-mRNA were exhibited. Taken together, our studies first revealed altered expression exosomal circRNA from plasma samples in patients with LUAD and support the need to explore their potential as biomarkers and pathological effects of lung cancer.


Exosomes are vesicles with sizes ranging from 30-150 nm. Analysis and detection of blood exosomes offer an effective service for cancer diagnosis, prognosis assessment, and evaluation of disease therapies. Because of the difference in separation procedures, methods of collection and use of anticoagulants, serum and plasma samples showed the diversity of the test results.

In order to evaluate the effect of exosomes isolation in serum and plasma samples, two methods commonly used insulation exosomal, ultracentrifugation and precipitation kit based polymers, used, respectively. And the insulating effect was evaluated by comparing the composition and abundant proteins from exosomes isolated based on the analysis of MS-based proteomics. The results showed that plasma exosomes extracted by ultracentrifugation more exosome identify biomarkers and biomarker concentration is higher than others.

And plasma exosomes could be a good example for the blood-based proteomics study of exosomes. It would be useful for future targeted biomarker discovery. This article is protected by copyright. All rights reserved.

 Circular RNAs expression profiles in plasma exosomes from early-stage lung adenocarcinoma and the potential biomarkers.
Circular RNAs expression profiles in plasma exosomes from early-stage lung adenocarcinoma and the potential biomarkers.

Exosomes molecular diagnostics: direct conversion of exosomes into cDNA for gene amplification by polymerase chain reaction two steps.

Exosomes are cells derived lipid nanoparticles with a size of 30-100 nm in diameter, was found in almost all biological fluids. The composition of exosomes mainly lipids, proteins, RNA, DNA, and non-coding RNA. At present, most of the available methods and commercial kits for exosomal RNA (Exo-RNA) isolation has its limitations and shortcomings.

Starting small volume of exosomes and the use of extraction / filtration column is usually not enough outcome results exosomal RNA after isolation. The majority of RNA contained in exosomes purified various sizes 15-500 nucleotides. Some RNA isolation kit is suitable for the isolation of small RNA transcripts larger but difficult to detect mRNA transcripts. For all kits, gift cost per sample analyzed is very high.

Lentivirus Precipitation Solution

VC150 500 ml
EUR 814.8

Retrovirus Precipitation Solution

VC200 100 ml
EUR 194.04

Retrovirus Precipitation Solution

VC225 250 ml
EUR 432.6

Retrovirus Precipitation Solution

VC250 500 ml
EUR 814.8

Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL)

TMEXO-1 100 reactions
EUR 522

PEG-it Virus Precipitation Solution (250 mL aliquot)

LV825A-1 250 ml
EUR 711

PEG-it Virus Precipitation Solution (100 mL aliquot)

LV810A-1 100 ml
EUR 325

ExoQuick Exosome RNA Column Purification kit

EQ808A-1 20 preps
EUR 344

TMB for Precipitation

42R-TB100x 1000 ml
EUR 645.6
Description: High sensitivity TMB Substrate for Precipitation assays

ExoQuick-LP for lipoprotein pre-clear & exosome isolation

EXOLP5A-1 5 reactions
EUR 473

DNA Precipitation Buffer

D106 55 ml
EUR 210
Description: DNA Precipitation Buffer by Cygnus Technologies is available in Europe via Gentaur.

PEG Virus Precipitation Kit

K904-200 each
EUR 1201.2

PEG Virus Precipitation Kit

K904-50 each
EUR 451.2

PEG Virus Precipitation Kit

K2190-200 200 preparations
EUR 1270.8

PEG Virus Precipitation Kit

K2190-50 50 preparations
EUR 486

50ML Protein Precipitation Kit

NAT1254 50ML
EUR 309.6

Chicken IgY precipitation reagent

85R-1010 4 ml
EUR 664.8
Description: Chicken IgY precipitation reagent for use in immunoassays

In Vivo SDS-K+ Precipitation Kit

TG1011-1 100 assays
EUR 362.4

In Vivo SDS-K+ Precipitation Kit

TG1011-2 250 assays
EUR 496.8

In Vitro SDS-K+ Precipitation Kit

TG1010-1 100 assays
EUR 550.8

In Vitro SDS-K+ Precipitation Kit

TG1010-2 250 assays
EUR 685.2

Nucleic Acid Precipitation Enhancer

TBS6010 2 x0.5 mL
EUR 98

MinuteTM Native Protein Precipitation Kit from Saliva (20 tests)

SV-043 each
EUR 330

ExoQuick-CG cGMP-grade Exosome Isolation reagent (50 ml)

EXOCG50A-1 50 ml
EUR 1106

ExoQuick PLUS Exosome Purification Kit - for serum and plasma

EQPL10A-1 10 reactions
EUR 518

Kinesis TELOS PPT Protein Precipitation Plate

CHR6509 EACH
EUR 105.75

ExoQuick Exosome Isolation and RNA Purification kit (for Serum and Plasma)

EQ806A-1 20 preps
EUR 493

Kinesis TELOS PPT Protein Precipitation Columns

CHR1378 PK100
EUR 222

101Bio High Efficiency Protein Precipitation kit

P5W6 - Ask for price

ExoQuick Exosome Isolation and RNA Purification kit (for Tissue Culture Media)

EQ806TC-1 10 preps
EUR 493

LPA (Linear Polyacrylamide) for DNA/RNA Precipitation

10510000-1 10 mg
EUR 85.98

LPA (Linear Polyacrylamide) for DNA/RNA Precipitation

10510000-2 25 mg
EUR 140.95

Minute TM High Efficiency Protein Precipitation kit

WA-006 each
EUR 160

ExoQuick-TC PLUS Exosome Purification Kit - for tissue culture media

EQPL10TC-1 10 reactions
EUR 518

SeraMir Exosome RNA Purification kit (5ml ExoQuick and 20 exoRNA columns)

RA806A-1 20 preps
EUR 493

SeraMir Exosome RNA Purification kit (10ml ExoQuick-TC and 10 exoRNA columns) 10 preps

RA806TC-1 10 preps
EUR 493

SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns)

RA800TC-1 10 reactions
EUR 595

Exo-Check Exosome Antibody Array

322-EXO-FBS-50A-1 100 µg
EUR 469.2

Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome)

P101EZ - Ask for price

Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome)

P100EZ - Ask for price

Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome) sample

P101EZ--sample - Ask for price

Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome) sample

P100EZ-sample - Ask for price

ExoQuick TC 10ml

EXOTC10A-1 10 ml
EUR 341

ExoQuick TC 50ml

EXOTC50A-1 50 ml
EUR 1147

Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800TC-1 with 50ml ExoQuick-TC, RA805A-1 and RA812A-1 components)

RA822TC-1 20 profiles
EUR 1533

SDS-Blue™ - Coomassie based solution for protein staining in SDS-PAGE

04-GSB
  • EUR 144.00
  • EUR 96.00
  • 1L
  • 500mL
Description: SDS-Blue™ is an innovative patented formula, based on Coomassie blue, that comes in a convenient ready to use format for staining proteins in SDS-PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis). The formulation of SDS-Blue™ provides numerous advantages compared to the classic Coomassie staining or to other similar protein stains. SDS-Blue™ provides higher sensitivity, virtually no background and eliminates the need for destaining of the gel due to its high specificity and affinity to bind to protein only. Not only does SDS-Blue™ yield clear and sharp bands, but it also contains no methanol and acetic acid, making it non-hazardous, safe to handle and friendly to the environment when disposed of. Two other advantages that make SDS-Blue™ the better option is that it is not light sensitive and can be stored at ambient temperature for 24 months. And this provides a considerable convenience, especially to laboratories that need and keep big amount of protein staining solutions – no more jammed refrigerators, you can keep SDS-Blue™ wherever it is most convenient for You!

ExoQuick ULTRA EV Isolation Kit for Serum and Plasma

EQULTRA-20A-1 20 reactions
EUR 941

TMB Precipitating (High Sensitivity)

TM5125 125 ml
EUR 102

TMB Precipitating (High Sensitivity)

TM5500 500 ml
EUR 165.6

TMB Precipitating (High Sensitivity)

TM5999 1000 ml
EUR 249.6

TMB Precipitating Reagent (For Membranes)

TMP125 125 ml
EUR 96

TMB Precipitating Reagent (For Membranes)

TMP500 500 ml
EUR 159.6

TMB Precipitating Reagent (For Membranes)

TMP999 1000 ml
EUR 236.4

TMB Precipitating (Standard Sensitivity)

TM3125 125 ml
EUR 96

TMB Precipitating (Standard Sensitivity)

TM3500 500 ml
EUR 156

TMB Precipitating (Standard Sensitivity)

TM3999 1000 ml
EUR 235.2

Universal Protein Precipitating Reagent

BSP012 100preps
EUR 97.58

ExoQuick-TC ULTRA EV Isolation Kit for Tissue Culture Media

EQULTRA-20TC-1 20 reactions
EUR 518

Schillers Test Solution

RRSP2536-D 500ml
EUR 10.19

Schillers Test Solution

RRSP2536-E 1L
EUR 14.76

Gentamicin Solution, Gentamicin Solution, 50 mg/mL

CCM1123-010 10 mL
EUR 76.86

Dissolution Test Solution 2

08590-66 20L
EUR 75.6

Calcium resinate (precipitated)

C01800 25G
EUR 132.08

Porcine Parvovirus Antibody Elisa Test Kit

767-LSY-30009 192 Wells/kit
EUR 382

Calcium carbonate, 99%, precipitated, Ph. Eur., BP, USP, FCC grade

GX0868-1 1
EUR 33.1

Disintegration Test Solution 2(pH 6.8)

08589-06 20L
EUR 88.9

GENTAMICIN SOLUTION (100 mg/mL)

G3410 100ML
EUR 80.07

GENTAMYCIN SULFATE SOLUTION

IB02030 -
EUR 64.1
Description: 50MG/ML-20ML (REF)

GENTAMICIN SOLUTION (50 mg/mL)

G3350 100ML
EUR 60

Disintegration Test Solution 2(pH 6.8)(10x)

08593-07 5L
EUR 68.6

Disintegration Test Solution 2(pH 6.8)(10x)

08593-36 20L
EUR 192.5

Gentamycin/Amphotericin B Solution(100X)

CA700-050 500ml
EUR 208.8

Gentamicin Sulfate Solution(50mg/ml)

11980-14 10ML
EUR 56

Gentamicin Sulfate Solution(10mg/ml)

16672-04 10ML
EUR 16.1

10mg/mL Gentamicin Sulphate Solution

PB180124-10mL 10 mL
EUR 15
Description: Supplements & Reagents

Gentamicin Sulfate Solution, 100mg/mL, Sterile

40120797-1 20 mL
EUR 59.15

Gentamicin Sulfate Solution, 100mg/mL, Sterile

40120797-2 100 mL
EUR 148.6

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Cell Media, Luminometric)

K1210-100 each
EUR 1161.6

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Cell media, Colorimetric)

K1205-100 each
EUR 1161.6

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Urine, Colorimetric)

K1202-100 each
EUR 1161.6

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Serum, Colorimetric)

K1203-100 each
EUR 1161.6

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Plasma, Luminometric)

K1206-100 each
EUR 1161.6

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Urine, Luminometric)

K1207-100 each
EUR 1161.6

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Serum, Luminometric)

K1208-100 each
EUR 1161.6

*Innovative Grade US Origin Rabbit Barium Chloride Precipitate Plasma Lyophilized

IRBBACL2PPLALY500ML each
EUR 1045
Description: *Innovative Grade US Origin Rabbit Barium Chloride Precipitate Plasma Lyophilized

*Innovative Grade US Origin Rabbit Barium Chloride Precipitate Plasma Lyophilized

IRBBACL2PPLALY50ML each
EUR 150
Description: *Innovative Grade US Origin Rabbit Barium Chloride Precipitate Plasma Lyophilized

Gentamicin Sulfate Solution, 10mg/mL, Sterile

40120796-1 20x1 mL
EUR 58.57

Gentamicin Sulfate Solution, 10mg/mL, Sterile

40120796-2 20 mL
EUR 53.99

Gentamicin Sulfate Solution, 10mg/mL, Sterile

40120796-3 50 mL
EUR 77.39

Gentamicin Sulfate Solution, 10mg/mL, Sterile

40120796-4 100 mL
EUR 116.22

Gentamicin Sulfate Solution, 10mg/mL, Sterile

40120796-5 100 mL
EUR 73.7

Gentamicin Sulfate Solution, 30mg/mL, Sterile

40120798-1 20 mL
EUR 37.61

Gentamicin Sulfate Solution, 30mg/mL, Sterile

40120798-2 100 mL
EUR 75.2

Gentamicin Sulfate Solution, 50mg/mL, Sterile

40710024-1 20 mL
EUR 44.92

Gentamicin Sulfate Solution, 50mg/mL, Sterile

40710024-2 100 mL
EUR 112.16

Gentamicin Sulfate Solution (50 mg/mL in Water)

G046-20ML 20 mL
EUR 132

ExoQuant? Overall Exosome Capture and Quantification ELISA Assay Kit (Human Plasma, Colorimetric)

K1201-100 each
EUR 1161.6

Exosome Isolation kit (for serum & plasma -Exosome-RNA-NGS)

P101NGS - Ask for price

Psoriasis Exosome

P141-PS - Ask for price

Gentian Violet Solution

GVS125 125 ml
EUR 100.8

Gentian Violet Solution

GVS500 500 ml
EUR 156

Gentian Violet Solution

GVS999 1000 ml
EUR 213.6

Morpheus - Precipitant Mix 4, 100mL *SHIPPED ON ICE*

M-MD2-100-84 each
EUR 312
Description: Morpheus - Precipitant Mix 4, 100mL *SHIPPED ON ICE*

ExoQuant? Tumor-derived Exosome enrichment and Quantification Assay Kit (Biological Fluids/Cell Media, Colorimetric)

K1204-100 each
EUR 1161.6

ExoQuant? Tumor-derived Exosome enrichment and Quantification Assay Kit (Biological Fluids/Cell Media, Luminometric)

K1209-100 each
EUR 1161.6

Lung Cancer Exosome

P141-LG - Ask for price

Leukemia - ALL Exosome

P141-ALL - Ask for price

Leukemia - AML Exosome

P141-AML - Ask for price

Leukemia - CLL Exosome

P141-CLL - Ask for price

Leukemia - CML Exosome

P141-CML - Ask for price

Liver Cancer Exosome

P141-LV - Ask for price

Exosome FACS sorting

S107 -
EUR 2000

Wizard Precipitant Synergy - Tubes (Precipitant Synergy Protein Crystallization Screen Kit), 192 formulations, 1.7 mL volumes in tubes

M-R-1009539 192 X 1.7 ml
EUR 2064
Description: Wizard Precipitant Synergy - Tubes (Precipitant Synergy Protein Crystallization Screen Kit), 192 formulations, 1.7 mL volumes in tubes

Breast Cancer Exosome

P141-BR - Ask for price

Crohn's Disease Exosome

P141-CR - Ask for price

Kidney Cancer Exosome

P141-KN - Ask for price

Psoriasis Exosome RNA

P241-PS - Ask for price

Exosome Isolation Kit

E601E001 20 tests
EUR 2500

Bladder Cancer Exosome

P141-BL - Ask for price

Thyroid Cancer Exosome

P141-TH - Ask for price

Prostate Cancer Exosome

P141-PR - Ask for price

Exosome Biotin labeling

S106 -
EUR 800

Exosome (Pre-isolated) RNA Extraction kit Note! This kit doesn't contain the conponents for exosome enrichment.

EIRK-01 50 reactions
EUR 3380.4
Description: A RNA Extraction kit that is ideal for total RNA (miRNA+mRNAs) extraction from pre-isolated exosomes.

Gentodenz

19-DENZ-500 500 g
EUR 416

Colorectal Cancer Exosome

P141-CO - Ask for price

Exosome antibody labeling

S105 -
EUR 800

Lung Cancer Exosome RNA

P241-LG - Ask for price

Diabetes (Type 1) Exosome

P141-DB1 - Ask for price

Diabetes (Type 2) Exosome

P141-DB2 - Ask for price

Normal human exosome RNA

P240 - Ask for price

Leukemia - ALL Exosome RNA

P241-ALL - Ask for price

Leukemia - AML Exosome RNA

P241-AML - Ask for price

Leukemia - CLL Exosome RNA

P241-CLL - Ask for price

Leukemia - CML Exosome RNA

P241-CML - Ask for price

Liver Cancer Exosome RNA

P241-LV - Ask for price

Exosome-TEM-easy Kit

P130 - Ask for price

Exo-APC Exosome FACS stain

EXOFLOW810A-1 20 reactions
EUR 188

EXOCET Exosome Quantitation Kit

EXOCET96A-1 96 reactions
EUR 462

Breast Cancer Exosome RNA

P241-BR - Ask for price

Crohn's Disease Exosome RNA

P241-CR - Ask for price

Kidney Cancer Exosome RNA

P241-KN - Ask for price

Exo-FITC Exosome FACS stain

EXOFLOW800A-1 20 reactions
EUR 188

Rheumatoid Arthritis Exosome

P141-RA - Ask for price

Exosome fluorescent staining

S104 -
EUR 800

Bladder Cancer Exosome RNA

P241-BL - Ask for price

Thyroid Cancer Exosome RNA

P241-TH - Ask for price

Lyophilized Exosome Standards

ExoA375 100 μg
EUR 364.2

Lyophilized Exosome Standards

ExoHT29 100 μg
EUR 364.2

Lyophilized Exosome Standards

ExoMCF7 100 μg
EUR 364.2

Lyophilized Exosome Standards

ExoPC3 100 μg
EUR 364.2

Lyophilized Exosome Standards

ExoPLASMA 100 μg
EUR 364.2

Lyophilized Exosome Standards

ExoUrine 100 μg
EUR 364.2

Our current method provides a new way for the direct conversion of exosomes into cDNA synthesis (Exo-cDNA) and subsequent gene detection by the polymerase chain reaction (PCR). This method has several advantages over the established kit available. There is no extraction column is used in this procedure, which means a total recovery of RNA exosomal with maximum results.