Circular RNAs expression profiles in plasma exosomes from early-stage lung adenocarcinoma and the potential biomarkers.

This study aims to identify the circular RNA differential (circRNA) in exosomes plasma of patients with lung adenocarcinoma (LUAD) using high-throughput sequencing. First, exosomes were isolated using a kit exosome isolation and confirmed by Western blotting, transmission electron microscopy, and NanoSight Assay. Furthermore, the expression profile circRNA plasma is filtered by high-throughput sequencing and confirmed by fluorescence quantitative real-time polymerase chain reaction (qRT-PCR) and sequencing Sanger.

Finally, network-miRNA-mRNA circRNA performed to predict the potential function circRNAs. The results of the high-throughput sequencing data documented that exosomal circRNAs 182 differentially expressed in all screened, including 105 upregulated and 78 were downregulated in plasma LUAD patients compared with controls. Fourth circRNAs regulated including circ_0001492, circ_0001346, circ_0000690, and circ_0001439 synonymous with sequencing data by qRT-PCR, and the latent interaction circRNA-miRNA-mRNA were exhibited. Taken together, our studies first revealed altered expression exosomal circRNA from plasma samples in patients with LUAD and support the need to explore their potential as biomarkers and pathological effects of lung cancer.


Exosomes are vesicles with sizes ranging from 30-150 nm. Analysis and detection of blood exosomes offer an effective service for cancer diagnosis, prognosis assessment, and evaluation of disease therapies. Because of the difference in separation procedures, methods of collection and use of anticoagulants, serum and plasma samples showed the diversity of the test results.

In order to evaluate the effect of exosomes isolation in serum and plasma samples, two methods commonly used insulation exosomal, ultracentrifugation and precipitation kit based polymers, used, respectively. And the insulating effect was evaluated by comparing the composition and abundant proteins from exosomes isolated based on the analysis of MS-based proteomics. The results showed that plasma exosomes extracted by ultracentrifugation more exosome identify biomarkers and biomarker concentration is higher than others.

And plasma exosomes could be a good example for the blood-based proteomics study of exosomes. It would be useful for future targeted biomarker discovery. This article is protected by copyright. All rights reserved.

 Circular RNAs expression profiles in plasma exosomes from early-stage lung adenocarcinoma and the potential biomarkers.
Circular RNAs expression profiles in plasma exosomes from early-stage lung adenocarcinoma and the potential biomarkers.

Exosomes molecular diagnostics: direct conversion of exosomes into cDNA for gene amplification by polymerase chain reaction two steps.

Exosomes are cells derived lipid nanoparticles with a size of 30-100 nm in diameter, was found in almost all biological fluids. The composition of exosomes mainly lipids, proteins, RNA, DNA, and non-coding RNA. At present, most of the available methods and commercial kits for exosomal RNA (Exo-RNA) isolation has its limitations and shortcomings.

Starting small volume of exosomes and the use of extraction / filtration column is usually not enough outcome results exosomal RNA after isolation. The majority of RNA contained in exosomes purified various sizes 15-500 nucleotides. Some RNA isolation kit is suitable for the isolation of small RNA transcripts larger but difficult to detect mRNA transcripts. For all kits, gift cost per sample analyzed is very high.

Exosome precipitation from Plasma + Thrombin

EPStep-T 5 ml
EUR 336

ExoQuick Exosome RNA Column Purification kit

EQ808A-1 20 preps
EUR 402

Exosome precipitation from Plasma and Serum

EPStep-PS 5 ml
EUR 297

ExoQuick-LP for lipoprotein pre-clear & exosome isolation

EXOLP5A-1 5 reactions
EUR 533
  • Category: Exosomes

PEG-it Virus Precipitation Solution (250 mL aliquot)

LV825A-1 250 ml
EUR 777
  • Category: Lentiviral Technology

PEG-it Virus Precipitation Solution (100 mL aliquot)

LV810A-1 100 ml
EUR 382
  • Category: Lentiviral Technology

ExoQuick-CG cGMP-grade Exosome Isolation reagent (50 ml)

EXOCG50A-1 50 ml
EUR 1181
  • Category: Exosomes

ExoQuick PLUS Exosome Purification Kit - for serum and plasma

EQPL10A-1 10 reactions
EUR 579
  • Category: Exosomes

ExoQuick-TC PLUS Exosome Purification Kit - for tissue culture media

EQPL10TC-1 10 reactions
EUR 579
  • Category: Exosomes

NATtrol Zika Virus Stock (Qualitative) (1 mL)

TEST 1 mL
EUR 1106.64
  • What is the product classification?
  • NATtrol Zika Virus Stock Qualitative is marked as RUO.
Description: Please contact Gentaur in order to receive the datasheet of the product.

ExoQuick Exosome Isolation and RNA Purification kit (for Serum and Plasma)

EQ806A-1 20 preps
EUR 554

ExoQuick Exosome Isolation and RNA Purification kit (for Tissue Culture Media)

EQ806TC-1 10 preps
EUR 554

SeraMir Exosome RNA Purification kit (5ml ExoQuick and 20 exoRNA columns)

RA806A-1 20 preps
EUR 554
  • Category: MicroRNA Tools

Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL)

TMEXO-1 100 reactions
EUR 583
  • Category: Exosomes

Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome)

P101EZ NULL
EUR 0

ExoQuick TC 10ml

EXOTC10A-1 10 ml
EUR 399
  • Category: Exosomes

ExoQuick TC 50ml

EXOTC50A-1 50 ml
EUR 1222
  • Category: Exosomes

TMB for Precipitation

42R-TB100x 1000 ml
EUR 538
Description: High sensitivity TMB Substrate for Precipitation assays

DNA Precipitation Buffer

D106 55 ml
EUR 175
  • Product category: DNA and RNA extraction and purification
Description: DNA Precipitation Buffer by Cygnus Technologies is available in Europe via Gentaur.

Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome)

P100EZ NULL
EUR 0

Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome) sample

P101EZ--sample NULL
EUR 0

Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome) sample

P100EZ-sample NULL
EUR 0

SeraMir Exosome RNA Purification kit (10ml ExoQuick-TC and 10 exoRNA columns) 10 preps

RA806TC-1 10 preps
EUR 554
  • Category: MicroRNA Tools

Chicken IgY precipitation reagent

85R-1010 4 ml
EUR 554
Description: Chicken IgY precipitation reagent for use in immunoassays

PEG Virus Precipitation Kit

K904-200
EUR 1001

PEG Virus Precipitation Kit

K904-50
EUR 376

PEG Virus Precipitation Kit

K2190-200 200 preparations
EUR 1059

PEG Virus Precipitation Kit

K2190-50 50 preparations
EUR 405

50ML Protein Precipitation Kit

NAT1254 50ML
EUR 225

SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns)

RA800TC-1 10 reactions
EUR 659
  • Category: MicroRNA Tools

101Bio High Efficiency Protein Precipitation kit

P5W6 NULL
EUR 0

In Vitro SDS-K+ Precipitation Kit

TG1010-1 100 assays
EUR 459

In Vitro SDS-K+ Precipitation Kit

TG1010-2 250 assays
EUR 571

In Vivo SDS-K+ Precipitation Kit

TG1011-1 100 assays
EUR 302

In Vivo SDS-K+ Precipitation Kit

TG1011-2 250 assays
EUR 414

Accu-Tell COVID-19 IgG/IgM Rapid Test

GEN-B352-20tests 20 tests
EUR 236
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

Accu-Tell COVID-19 IgG/IgM Rapid Test

GEN-B352-40tests 40 tests
EUR 321
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

Psoriasis Exosome

P141-PS NULL
EUR 0

DNASE TEST AGAR

D04-101-10kg 10 kg Ask for price

DNASE TEST AGAR

D04-101-2Kg 2 Kg Ask for price

DNASE TEST AGAR

D04-101-500g 500 g Ask for price

DERMATOPHYTE TEST AGAR

D04-107-10kg 10 kg
EUR 849

DERMATOPHYTE TEST AGAR

D04-107-2Kg 2 Kg
EUR 224

DERMATOPHYTE TEST AGAR

D04-107-500g 500 g
EUR 97

MOTILITY TEST MEDIUM

M13-121-10kg 10 kg
EUR 1528

MOTILITY TEST MEDIUM

M13-121-2Kg 2 Kg
EUR 371

MOTILITY TEST MEDIUM

M13-121-500g 500 g
EUR 137

SNYDER TEST AGAR

S19-119-10kg 10 kg
EUR 1049

SNYDER TEST AGAR

S19-119-2Kg 2 Kg
EUR 267

SNYDER TEST AGAR

S19-119-500g 500 g
EUR 109

SAM Test Strip

TS00201s-30 30 tests
EUR 416
Description: S-adenosylmethionine quantitative test strip for serum, plasma and whole blood

SAH Test Strip

TS00301s-30 30 tests
EUR 504
Description: S-adenosylhomocysteine quantitative test strip for serum, plasma and whole blood

HCY Test Strip

TS00401s-30 30 tests
EUR 553
Description: Serum homocysteine quantitative test strip

Sodium Pyruvate Solution, 100 mM Solution

CCM1753-100 100 mL
EUR 60.84
  • Product category: Culture Media

Lyophilized Exosome Standards

ExoA375 100 μg
EUR 303.5

Lyophilized Exosome Standards

ExoHT29 100 μg
EUR 303.5

Lyophilized Exosome Standards

ExoMCF7 100 μg
EUR 303.5

Lyophilized Exosome Standards

ExoPC3 100 μg
EUR 303.5

Lyophilized Exosome Standards

ExoPLASMA 100 μg
EUR 303.5

Lyophilized Exosome Standards

ExoUrine 100 μg
EUR 303.5

Leukemia - ALL Exosome

P141-ALL NULL
EUR 0

Leukemia - AML Exosome

P141-AML NULL
EUR 0

Bladder Cancer Exosome

P141-BL NULL
EUR 0

Breast Cancer Exosome

P141-BR NULL
EUR 0

Leukemia - CLL Exosome

P141-CLL NULL
EUR 0

Leukemia - CML Exosome

P141-CML NULL
EUR 0

Colorectal Cancer Exosome

P141-CO NULL
EUR 0

Crohn's Disease Exosome

P141-CR NULL
EUR 0

Kidney Cancer Exosome

P141-KN NULL
EUR 0

Lung Cancer Exosome

P141-LG NULL
EUR 0

Liver Cancer Exosome

P141-LV NULL
EUR 0

Nasopharyngeal Cancer Exosome

P141-NA NULL
EUR 0

Prostate Cancer Exosome

P141-PR NULL
EUR 0

Rheumatoid Arthritis Exosome

P141-RA NULL
EUR 0

Thyroid Cancer Exosome

P141-TH NULL
EUR 0

Psoriasis Exosome RNA

P241-PS NULL
EUR 0

2019-nCoV IgG/IgM Rapid Test Cassette (Whole Blood/Serum/Plasma)

GEN-402-25tests 25 tests
EUR 244
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

SDS Solution

2102-100
EUR 137

EDTA Solution

2103-100
EUR 137

Denaturing solution

DE540 1L
EUR 61.31
  • Product category: Electrophoresis Related/Reagents

TRIMMING SOLUTION

99-676-CM 1 L /pk
EUR 88
Description: Specialty Media; Islet products

AlphaBioCoat Solution

AC001 20 ml
EUR 271

Borax Solution

BOR015 15 ml
EUR 56

Borax Solution

BOR125 125 ml
EUR 79

Borax Solution

BOR500 500 ml
EUR 119

Borax Solution

BOR999 1000 ml
EUR 183

Stop Solution

F006 12 ml
EUR 180
  • Product category: Buffer solution
Description: Stop Solution by Cygnus Technologies is available in Europe via Gentaur.

Prehybridization Solution

K2191050-1 5 ml
EUR 137
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Hybridization Solution

K2191050-2 5 ml
EUR 157
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Blocking Solution

K2191050-8 250 ul
EUR 137
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Orcein Solution

OAA125 125 ml
EUR 66

Orcein Solution

OAA500 500 ml
EUR 109

Orcein Solution

OAA999 1000 ml
EUR 148

Methenamine Solution

MET125 125 ml
EUR 64

Methenamine Solution

MET500 500 ml
EUR 95

Methenamine Solution

MET999 1000 ml
EUR 132

DTT Solution

R-1104 1 ml, 500 mM
EUR 109.45
Description: fast delivery possible

Tartrazine Solution

TZQ125 125 ml
EUR 64

Tartrazine Solution

TZQ500 500 ml
EUR 98

Tartrazine Solution

TZQ999 1000 ml
EUR 127

Schiff's Solution

SRF030 30 ml
EUR 64

Schiff's Solution

SRF250 250 ml
EUR 77

Schiff's Solution

SRF3800 1 Gal.
EUR 345

Schiff's Solution

SRF500 500 ml
EUR 103

Schiff's Solution

SRF999 1000 ml
EUR 149

Exosome Isolation kit (for serum & plasma -Exosome-RNA-NGS)

P101NGS NULL
EUR 0

50ml TC Tubes, Conical, 440 units/box

04-5540150 440 units/box
EUR 71

GMP IL4, 50µg

04-GMP-HU-IL4-50UG 50µg
EUR 483
Description: Recombinant Human IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15000 Dalton. The rHuIL-4 is purified by proprietary chromatographic techniques.

rHu IL 2 , 3MIU

04-RHIL2-02F01 1 vial
EUR 249
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated.

rHu IL 2 , 3MIU , Lot 200908F02

04-RHIL2-08F02 1 vial
EUR 249
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated.

PRE-GMP rHu GM-CSF, Molgramostim-Leukoma

04-RHUGM-CSF-7A10 300 µg
EUR 385
Description: Recombinant human GM-CSF produced in E.coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids, two pairs of disulfide bonds and having a molecular mass of approximately 14.5kD.

Gentamicin Solution, Gentamicin Solution, 50 mg/mL

CCM1123-010 10 mL
EUR 64.05
  • Product category: Culture Media

CA72-4 ELISA test

24 96T/Box Ask for price
  • Area of application: Hormone testing
Description: ELISA based test for quantitative detection of CA72-4

FERR ( Ferritin) ELISA test

7 96T/Box Ask for price
  • Area of application: Hormone testing
Description: ELISA based test for quantitative detection of FERR ( Ferritin)

TSH (Thyrotrophin) ELISA test

14 96T/Box Ask for price
  • Area of application: Hormone testing
Description: ELISA based test for quantitative detection of TSH (Thyrotrophin)

T3 (Triiodothyronine) ELISA test

15 96T/Box Ask for price
  • Area of application: Hormone testing
Description: ELISA based test for quantitative detection of T3 (Triiodothyronine)

T4 (Thyroxine) ELISA test

16 96T/Box Ask for price
  • Area of application: Hormone testing
Description: ELISA based test for quantitative detection of T4 (Thyroxine)

TESTO (Testosterone) ELISA test

19 96T/Box Ask for price
  • Area of application: Hormone testing
Description: ELISA based test for quantitative detection of O (osterone)

PROG (Progesterone) ELISA test

20 96T/Box Ask for price
  • Area of application: Hormone testing
Description: ELISA based test for quantitative detection of PROG (Progesterone)

E2 (Estradiol) ELISA test

5 96T/Box Ask for price
  • Area of application: Hormone testing
Description: ELISA based test for quantitative detection of E2 (Estradiol)

PRL (Prolactin) ELISA test

4 96T/Box Ask for price
  • Area of application: Hormone testing
Description: ELISA based test for quantitative detection of PRL (Prolactin)

Basic Cytotoxicity Test Kits

CT17001 125 Tests
EUR 409

Basic Cytotoxicity Test Kits

CT17002 250 Tests
EUR 650

Total Cytotoxicity Test Kits

CT17003 125 Tests
EUR 513

Total Cytotoxicity Test Kits

CT17004 250 Tests
EUR 813

Clearview Chlamydia MF test

CV504524 1 box
EUR 162
Description: Please check the datasheet of Clearview Chlamydia MF test before using the test.

PreS2-Ag ELISA test

96 96T/Box Ask for price
  • Area of application: Hepatitis testing
Description: ELISA based test for quantitative detection of PreS2-Ag

HBcAb Sandwich ELISA test

95 96T/Box Ask for price
  • Area of application: Hepatitis testing
Description: ELISA based test for quantitative detection of HBcAb Sandwich

Melamine Rapid Test Kit

abx092011-50tests 50 tests
EUR 370
  • Shipped within 5-12 working days.

NDV rapid test kit

RG15-03 1 box
EUR 139.05
Description: Please check the datasheet of NDV rapid test kit before using the test.

IBD rapid test strip

RG15-04 10 boxes
EUR 148
Description: Please check the datasheet of IBD rapid test strip before using the test.

Rabies rapid test strip

RG18-01 10 boxes
EUR 151.92
Description: Please check the datasheet of Rabies rapid test strip before using the test.

ExoQuick ULTRA EV Isolation Kit for Serum and Plasma

EQULTRA-20A-1 20 reactions
EUR 579

Exosome Component 5 Protein

20-abx260873
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 25 ug
  • 5 ug
  • Shipped within 5-10 working days.

Exosome Component 7 Protein

20-abx261347
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

Exosome Component 10 Protein

20-abx261805
  • EUR 4365.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

Exosome Component 4 Protein

20-abx261954
  • EUR 4490.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

Exosome Component 9 Protein

20-abx262123
  • EUR 328.00
  • EUR 5089.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

Exosome Component 3 Protein

20-abx263422
  • EUR 230.00
  • EUR 1609.00
  • EUR 328.00
  • 1 µg
  • 50 ug
  • 5 ug
  • Shipped within 5-10 working days.

Exosome Component 1 Protein

20-abx263423
  • EUR 1609.00
  • EUR 328.00
  • EUR 230.00
  • 100 ug
  • 10 ug
  • 2 µg
  • Shipped within 5-10 working days.

Exosome Component 8 Protein

20-abx262850
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

EXOCET Exosome Quantitation Kit

EXOCET96A-1 96 reactions
EUR 521
  • Category: Exosomes

FluoroCet Exosome Quantitation Kit

FCET96A-1 96 reactions
EUR 726
  • Category: Exosomes

Fluorescent Exosome Standard (Urine )

ESF-15 100 µg
EUR 1723
Description: Fluorescent exosomes are labeled with green dye, which offer a stable fluorescent labeling.

Exosome-TEM-easy Kit

P130 NULL
EUR 0

Diabetes (Type 1) Exosome

P141-DB1 NULL
EUR 0

Diabetes (Type 2) Exosome

P141-DB2 NULL
EUR 0

Leukemia - Hairy Cell Exosome

P141-HC NULL
EUR 0

Leukemia - Multiple Myeloma Exosome

P141-MM NULL
EUR 0

Melanoma (skin cancer) Exosome

P141-MN NULL
EUR 0

Non-Hodgkin lymphoma Exosome

P141-NH NULL
EUR 0

Systemic Lupus Erythematosus Exosome

P141-SL NULL
EUR 0

Our current method provides a new way for the direct conversion of exosomes into cDNA synthesis (Exo-cDNA) and subsequent gene detection by the polymerase chain reaction (PCR). This method has several advantages over the established kit available. There is no extraction column is used in this procedure, which means a total recovery of RNA exosomal with maximum results.