Circular RNAs expression profiles in plasma exosomes from early-stage lung adenocarcinoma and the potential biomarkers.

This study aims to identify the circular RNA differential (circRNA) in exosomes plasma of patients with lung adenocarcinoma (LUAD) using high-throughput sequencing. First, exosomes were isolated using a kit exosome isolation and confirmed by Western blotting, transmission electron microscopy, and NanoSight Assay. Furthermore, the expression profile circRNA plasma is filtered by high-throughput sequencing and confirmed by fluorescence quantitative real-time polymerase chain reaction (qRT-PCR) and sequencing Sanger.

Finally, network-miRNA-mRNA circRNA performed to predict the potential function circRNAs. The results of the high-throughput sequencing data documented that exosomal circRNAs 182 differentially expressed in all screened, including 105 upregulated and 78 were downregulated in plasma LUAD patients compared with controls. Fourth circRNAs regulated including circ_0001492, circ_0001346, circ_0000690, and circ_0001439 synonymous with sequencing data by qRT-PCR, and the latent interaction circRNA-miRNA-mRNA were exhibited. Taken together, our studies first revealed altered expression exosomal circRNA from plasma samples in patients with LUAD and support the need to explore their potential as biomarkers and pathological effects of lung cancer.


Exosomes are vesicles with sizes ranging from 30-150 nm. Analysis and detection of blood exosomes offer an effective service for cancer diagnosis, prognosis assessment, and evaluation of disease therapies. Because of the difference in separation procedures, methods of collection and use of anticoagulants, serum and plasma samples showed the diversity of the test results.

In order to evaluate the effect of exosomes isolation in serum and plasma samples, two methods commonly used insulation exosomal, ultracentrifugation and precipitation kit based polymers, used, respectively. And the insulating effect was evaluated by comparing the composition and abundant proteins from exosomes isolated based on the analysis of MS-based proteomics. The results showed that plasma exosomes extracted by ultracentrifugation more exosome identify biomarkers and biomarker concentration is higher than others.

And plasma exosomes could be a good example for the blood-based proteomics study of exosomes. It would be useful for future targeted biomarker discovery. This article is protected by copyright. All rights reserved.

 Circular RNAs expression profiles in plasma exosomes from early-stage lung adenocarcinoma and the potential biomarkers.
Circular RNAs expression profiles in plasma exosomes from early-stage lung adenocarcinoma and the potential biomarkers.

Exosomes molecular diagnostics: direct conversion of exosomes into cDNA for gene amplification by polymerase chain reaction two steps.

Exosomes are cells derived lipid nanoparticles with a size of 30-100 nm in diameter, was found in almost all biological fluids. The composition of exosomes mainly lipids, proteins, RNA, DNA, and non-coding RNA. At present, most of the available methods and commercial kits for exosomal RNA (Exo-RNA) isolation has its limitations and shortcomings.

Starting small volume of exosomes and the use of extraction / filtration column is usually not enough outcome results exosomal RNA after isolation. The majority of RNA contained in exosomes purified various sizes 15-500 nucleotides. Some RNA isolation kit is suitable for the isolation of small RNA transcripts larger but difficult to detect mRNA transcripts. For all kits, gift cost per sample analyzed is very high.

Lentivirus Precipitation Solution

VC150 500 ml
EUR 814.8

Retrovirus Precipitation Solution

VC200 100 ml
EUR 194.04

Retrovirus Precipitation Solution

VC225 250 ml
EUR 432.6

Retrovirus Precipitation Solution

VC250 500 ml
EUR 814.8

Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL)

TMEXO-1 100 reactions
EUR 522

PEG-it Virus Precipitation Solution (100 mL aliquot)

LV810A-1 100 ml
EUR 325

PEG-it Virus Precipitation Solution (250 mL aliquot)

LV825A-1 250 ml
EUR 711

TMB for Precipitation

42R-TB100x 1000 ml
EUR 473
Description: High sensitivity TMB Substrate for Precipitation assays

DNA Precipitation Buffer

D106 55 ml
EUR 210
Description: DNA Precipitation Buffer by Cygnus Technologies is available in Europe via Gentaur.

ExoQuick Exosome RNA Column Purification kit

EQ808A-1 20 preps
EUR 344

PEG Virus Precipitation Kit

K904-200 each
EUR 1201.2

PEG Virus Precipitation Kit

K904-50 each
EUR 451.2

PEG Virus Precipitation Kit

K2190-200 200 preparations
EUR 1270.8

PEG Virus Precipitation Kit

K2190-50 50 preparations
EUR 418
Description: High Viral Titer, No ultracentrifugation

ExoQuick-LP for lipoprotein pre-clear & exosome isolation

EXOLP5A-1 5 reactions
EUR 473

Chicken IgY precipitation reagent

85R-1010 4 ml
EUR 638
Description: Chicken IgY precipitation reagent for use in immunoassays

In Vivo SDS-K+ Precipitation Kit

TG1011-1 100 assays
EUR 362.4

In Vivo SDS-K+ Precipitation Kit

TG1011-2 250 assays
EUR 496.8

In Vitro SDS-K+ Precipitation Kit

TG1010-1 100 assays
EUR 550.8

In Vitro SDS-K+ Precipitation Kit

TG1010-2 250 assays
EUR 685.2

Nucleic Acid Precipitation Enhancer

TBS6010 2 x0.5 mL
EUR 98

50ML Protein Precipitation Kit - 50ML

NAT1254 50ML
EUR 348.3

MinuteTM Native Protein Precipitation Kit from Saliva (20 tests)

SV-043 each
EUR 330

PEG Virus Precipitation Kit - 200 preparations

GWB-AXR384 200 preparations Ask for price

PEG Virus Precipitation Kit - 50 preparations

GWB-AXR385 50 preparations Ask for price

101Bio High Efficiency Protein Precipitation kit

P5W6 30 ml / 30 ml
EUR 129

Kinesis TELOS PPT Protein Precipitation Plate - EACH

CHR6509 EACH
EUR 118.97

LPA (Linear Polyacrylamide) for DNA/RNA Precipitation

10510000-1 10 mg
EUR 85.98

LPA (Linear Polyacrylamide) for DNA/RNA Precipitation

10510000-2 25 mg
EUR 140.95

Minute TM High Efficiency Protein Precipitation kit

WA-006 each
EUR 160

ExoQuick-CG cGMP-grade Exosome Isolation reagent (50 ml)

EXOCG50A-1 50 ml
EUR 1106

ExoQuick PLUS Exosome Purification Kit - for serum and plasma

EQPL10A-1 10 reactions
EUR 518

Kinesis TELOS PPT Protein Precipitation Columns - PK100

CHR1378 PK100
EUR 249.75

ExoQuick Exosome Isolation and RNA Purification kit (for Serum and Plasma)

EQ806A-1 20 preps
EUR 493

ExoQuick Exosome Isolation and RNA Purification kit (for Tissue Culture Media)

EQ806TC-1 10 preps
EUR 493

ExoQuick-TC PLUS Exosome Purification Kit - for tissue culture media

EQPL10TC-1 10 reactions
EUR 518

SeraMir Exosome RNA Purification kit (5ml ExoQuick and 20 exoRNA columns)

RA806A-1 20 preps
EUR 493

SeraMir Exosome RNA Purification kit (10ml ExoQuick-TC and 10 exoRNA columns) 10 preps

RA806TC-1 10 preps
EUR 493

SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns)

RA800TC-1 10 reactions
EUR 595

Exo-Check Exosome Antibody Array

322-EXO-FBS-50A-1 100 µg
EUR 469.2

Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome)

P101EZ - Ask for price

Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome)

P100EZ - Ask for price

Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome) sample

P101EZ--sample - Ask for price

Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome) sample

P100EZ-sample - Ask for price

ExoQuick TC 10ml

EXOTC10A-1 10 ml
EUR 341

ExoQuick TC 50ml

EXOTC50A-1 50 ml
EUR 1147

Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800TC-1 with 50ml ExoQuick-TC, RA805A-1 and RA812A-1 components)

RA822TC-1 20 profiles
EUR 1533

SDS-Blue™ - Coomassie based solution for protein staining in SDS-PAGE

04-GSB
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  • 1L
  • 500mL
Description: SDS-Blue™ is an innovative patented formula, based on Coomassie blue, that comes in a convenient ready to use format for staining proteins in SDS-PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis). The formulation of SDS-Blue™ provides numerous advantages compared to the classic Coomassie staining or to other similar protein stains. SDS-Blue™ provides higher sensitivity, virtually no background and eliminates the need for destaining of the gel due to its high specificity and affinity to bind to protein only. Not only does SDS-Blue™ yield clear and sharp bands, but it also contains no methanol and acetic acid, making it non-hazardous, safe to handle and friendly to the environment when disposed of. Two other advantages that make SDS-Blue™ the better option is that it is not light sensitive and can be stored at ambient temperature for 24 months. And this provides a considerable convenience, especially to laboratories that need and keep big amount of protein staining solutions – no more jammed refrigerators, you can keep SDS-Blue™ wherever it is most convenient for You!

TMB Precipitating (High Sensitivity)

TM5125 125 ml
EUR 28.28

TMB Precipitating (High Sensitivity)

TM5500 500 ml
EUR 70.71

TMB Precipitating (High Sensitivity)

TM5999 1000 ml
EUR 127.28

TMB Precipitating Reagent (For Membranes)

TMP125 125 ml
EUR 96

TMB Precipitating Reagent (For Membranes)

TMP500 500 ml
EUR 159.6

TMB Precipitating Reagent (For Membranes)

TMP999 1000 ml
EUR 236.4

TMB Precipitating (Standard Sensitivity)

TM3125 125 ml
EUR 24.43

TMB Precipitating (Standard Sensitivity)

TM3500 500 ml
EUR 64.28

TMB Precipitating (Standard Sensitivity)

TM3999 1000 ml
EUR 117

Universal Protein Precipitating Reagent

BSP012 100preps
EUR 97.58

ExoQuick ULTRA EV Isolation Kit for Serum and Plasma

EQULTRA-20A-1 20 reactions
EUR 941

ExoQuick-TC ULTRA EV Isolation Kit for Tissue Culture Media

EQULTRA-20TC-1 20 reactions
EUR 518

ExoQualiTM Exosome DNA Extraction Kit (Pre-Isolated Exosomes)

CEIK-050L-30T 30T Ask for price

ExoQualiTM Exosome DNA Extraction Kit (Pre-Isolated Exosomes)

CEIK-050L-50T 50T Ask for price

ExoQualiTM Exosome RNA Extraction Kit (Pre-Isolated Exosomes)

CEIK-051L-30T 30T Ask for price

ExoQualiTM Exosome RNA Extraction Kit (Pre-Isolated Exosomes)

CEIK-051L-50T 50T Ask for price

Schillers Test Solution

RRSP2536-D 500ml
EUR 10.19

Schillers Test Solution

RRSP2536-E 1L
EUR 14.76

Gentamicin Solution, Gentamicin Solution, 50 mg/mL

CCM1123-010 10 mL
EUR 76.86

ExoQualiTM Exosome Isolation Kit (Urine)

CEIK-003L-10T 10 T Ask for price

ExoQualiTM Exosome Isolation Kit (Urine)

CEIK-003L-30T 30 T Ask for price

ExoQualiTM Exosome Isolation Kit (Serum)

CEIK-005L-10T 10 T Ask for price

ExoQualiTM Exosome Isolation Kit (Serum)

CEIK-005L-30T 30 T Ask for price

Silicic acid, precipitated

GRM622-500G 1 unit
EUR 13.55
Description: Silicic acid, precipitated

Sulphur precipitated, Hi-AR™

RM1316-500G 1 unit
EUR 11.59
Description: Sulphur precipitated, Hi-AR™

ExoQualiTM Exosome Isolation Kit (Plasma)

CEIK-004L-10T 10 T Ask for price

ExoQualiTM Exosome Isolation Kit (Plasma)

CEIK-004L-30T 30 T Ask for price

Dissolution Test Solution 2

08590-66 20L
EUR 75.6

ExoQualiTM Exosome Chromatography Columns

ESEC-001L-5columns 5 columns Ask for price

Calcium resinate (precipitated)

C01800 25G
EUR 132.08

ExoQualiTM Exosome Isolation Kit (Cell Media)

CEIK-001L-10T 10 T Ask for price

ExoQualiTM Exosome Isolation Kit (Cell Media)

CEIK-001L-30T 30 T Ask for price

ExoQualiTM Exosome Isolation Kit (All in one)

CEIK-008L-10T 10 T Ask for price

ExoQualiTM Exosome Isolation Kit (All in one)

CEIK-008L-30T 30 T Ask for price

ExoQualiTM Max Exosome Chromatography Columns

ESEC-002L-3columns 3 columns Ask for price

ExoQualiTM Exosome Isolation Kit (Plasma & Serum)

CEIK-006L-10T 10 T Ask for price

ExoQualiTM Exosome Isolation Kit (Plasma & Serum)

CEIK-006L-30T 30 T Ask for price

ExoQualiTM Mini Exosome Chromatography Columns

ESEC-003L-2columns 2 columns Ask for price

AlkaSept™ OPA Solution Test Strips

K116-1KT 1 unit
EUR 13.41
Description: AlkaSept™ OPA Solution Test Strips

ExoQualiTM Exosome Isolation Kit (Stem Cell Media)

CEIK-002L-10T 10 T Ask for price

ExoQualiTM Exosome Isolation Kit (Stem Cell Media)

CEIK-002L-30T 30 T Ask for price

Magnesium stearate precipitated fine pow

GRM7219-500G 1 unit
EUR 6.93
Description: Magnesium stearate precipitated fine pow

Porcine Parvovirus Antibody Elisa Test Kit

767-LSY-30009 192 Wells/kit
EUR 382

ExoQualiTM Exosome Isolation Kit (other body fluids)

CEIK-007L-10T 10 T Ask for price

ExoQualiTM Exosome Isolation Kit (other body fluids)

CEIK-007L-30T 30 T Ask for price

ExoQualiTM Exosome DNA Extraction Kit (Plasma & Serum)

CEIK-048L-30T 30T Ask for price

ExoQualiTM Exosome DNA Extraction Kit (Plasma & Serum)

CEIK-048L-50T 50T Ask for price

ExoQualiTM Exosome RNA Extraction Kit (Plasma & Serum)

CEIK-093L-30T 30T Ask for price

ExoQualiTM Exosome RNA Extraction Kit (Plasma & Serum)

CEIK-093L-50T 50T Ask for price

Calcium carbonate, 99%, precipitated, Ph. Eur., BP, USP, FCC grade

GX0868-1 1
EUR 33.1

GENTAMICIN SOLUTION (100 mg/mL)

G3410 100ML
EUR 80.07

ExoQuali™ Lyophilized Exosome Standards ( Human Serum )

CEIK-070L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Serum )

CEIK-070L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Urine )

CEIK-071L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Urine )

CEIK-071L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Plasma )

CEIK-068L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Plasma )

CEIK-068L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Saliva )

CEIK-069L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Saliva )

CEIK-069L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

Disintegration Test Solution 2(pH 6.8)

08589-06 20L
EUR 88.9

ExoQuali™ Lyophilized Exosome Standards ( MM1 cell line )

CEIK-064L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( MM1 cell line )

CEIK-064L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( PC3 cell line )

CEIK-065L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( PC3 cell line )

CEIK-065L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from serum)

CEIK-044L-30T 30 T Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from serum)

CEIK-044L-50T 50 T Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from urine)

CEIK-046L-10T 10 T Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from urine)

CEIK-046L-24T 24 T Ask for price

GENTAMYCIN SULFATE SOLUTION

IB02030 -
EUR 64.1
Description: 50MG/ML-20ML (REF)

ExoQualiTM Overall Exosome Isolation Reagent (from plasma)

CEIK-045L-30T 30 T Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from plasma)

CEIK-045L-50T 50 T Ask for price

ExoQuali™ Lyophilized Exosome Standards ( A549 cell line )

CEIK-054L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( A549 cell line )

CEIK-054L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

Gentamicin Solution, 50mg/mL

25-533 10 mL/Unit
EUR 19.67
Description: Sterile Solution

ExoQualiTM Total Exosome Capture & Quantification Kit (Serum)

CEIK-107L-96wells 96 wells Ask for price

ExoQualiTM Total Exosome Capture & Quantification Kit (Urine)

CEIK-110L-96wells 96 wells Ask for price

ExoQuali™ Lyophilized Exosome Standards ( COLO1 cell line )

CEIK-058L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( COLO1 cell line )

CEIK-058L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( U87 MG cell line )

CEIK-067L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( U87 MG cell line )

CEIK-067L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

GENTAMICIN SOLUTION (50 mg/mL)

G3350 100ML
EUR 60

ExoQualiTM Total Exosome Capture & Quantification Kit (Plasma)

CEIK-108L-96wells 96 wells Ask for price

ExoQuali™ Lyophilized Exosome Standards ( B16F10 cell line )

CEIK-055L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( B16F10 cell line )

CEIK-055L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( BLCL21 cell line )

CEIK-056L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( BLCL21 cell line )

CEIK-056L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( HCT116 cell line )

CEIK-060L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( HCT116 cell line )

CEIK-060L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( BPH-1 cell line )

CEIK-057L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( BPH-1 cell line )

CEIK-057L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( K-562 cell line )

CEIK-062L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( K-562 cell line )

CEIK-062L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQualiTM Overall Exosome Isolation Immunoplates (from Serum)

CEIK-023L-96wells 96 wells Ask for price

ExoQualiTM Overall Exosome Isolation Immunoplates (from Saliva)

CEIK-026L-96wells 96 wells Ask for price

ExoQualiTM Overall Exosome Isolation Immunoplates (from Plasma)

CEIK-095L-96wells 96 wells Ask for price

ExoQualiTM Overall Exosome Isolation Immunoplates (from Urine)

CEIK-096L-96wells 96 wells Ask for price

ExoQualiTM Exosome DNA Extraction Kit (Urine & Cell Supernatant)

CEIK-049L-10T 10T Ask for price

ExoQualiTM Exosome DNA Extraction Kit (Urine & Cell Supernatant)

CEIK-049L-20T 20T Ask for price

ExoQualiTM Exosome RNA Extraction Kit (Urine & Cell Supernatant)

CEIK-094L-10T 10T Ask for price

ExoQualiTM Exosome RNA Extraction Kit (Urine & Cell Supernatant)

CEIK-094L-20T 20T Ask for price

Disintegration Test Solution 2(pH 6.8)(10x)

08593-07 5L
EUR 68.6

Disintegration Test Solution 2(pH 6.8)(10x)

08593-36 20L
EUR 192.5

Gentamicin ReadyMade™ Solution

G046-10x1ML 10 x 1 mL
EUR 51.25
Description: 1405-41-1

ExoQuali™ Lyophilized Exosome Standards ( SK-N-SH cell line )

CEIK-066L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

Our current method provides a new way for the direct conversion of exosomes into cDNA synthesis (Exo-cDNA) and subsequent gene detection by the polymerase chain reaction (PCR). This method has several advantages over the established kit available. There is no extraction column is used in this procedure, which means a total recovery of RNA exosomal with maximum results.