This study aims to identify the circular RNA differential (circRNA) in exosomes plasma of patients with lung adenocarcinoma (LUAD) using high-throughput sequencing. First, exosomes were isolated using a kit exosome isolation and confirmed by Western blotting, transmission electron microscopy, and NanoSight Assay. Furthermore, the expression profile circRNA plasma is filtered by high-throughput sequencing and confirmed by fluorescence quantitative real-time polymerase chain reaction (qRT-PCR) and sequencing Sanger.

Finally, network-miRNA-mRNA circRNA performed to predict the potential function circRNAs. The results of the high-throughput sequencing data documented that exosomal circRNAs 182 differentially expressed in all screened, including 105 upregulated and 78 were downregulated in plasma LUAD patients compared with controls. Fourth circRNAs regulated including circ_0001492, circ_0001346, circ_0000690, and circ_0001439 synonymous with sequencing data by qRT-PCR, and the latent interaction circRNA-miRNA-mRNA were exhibited. Taken together, our studies first revealed altered expression exosomal circRNA from plasma samples in patients with LUAD and support the need to explore their potential as biomarkers and pathological effects of lung cancer.

Exosomes are vesicles with sizes ranging from 30-150 nm. Analysis and detection of blood exosomes offer an effective service for cancer diagnosis, prognosis assessment, and evaluation of disease therapies. Because of the difference in separation procedures, methods of collection and use of anticoagulants, serum and plasma samples showed the diversity of the test results.

In order to evaluate the effect of exosomes isolation in serum and plasma samples, two methods commonly used insulation exosomal, ultracentrifugation and precipitation kit based polymers, used, respectively. And the insulating effect was evaluated by comparing the composition and abundant proteins from exosomes isolated based on the analysis of MS-based proteomics. The results showed that plasma exosomes extracted by ultracentrifugation more exosome identify biomarkers and biomarker concentration is higher than others.

And plasma exosomes could be a good example for the blood-based proteomics study of exosomes. It would be useful for future targeted biomarker discovery. This article is protected by copyright. All rights reserved.

Exosomes molecular diagnostics: direct conversion of exosomes into cDNA for gene amplification by polymerase chain reaction two steps.

Exosomes are cells derived lipid nanoparticles with a size of 30-100 nm in diameter, was found in almost all biological fluids. The composition of exosomes mainly lipids, proteins, RNA, DNA, and non-coding RNA. At present, most of the available methods and commercial kits for exosomal RNA (Exo-RNA) isolation has its limitations and shortcomings.

Starting small volume of exosomes and the use of extraction / filtration column is usually not enough outcome results exosomal RNA after isolation. The majority of RNA contained in exosomes purified various sizes 15-500 nucleotides. Some RNA isolation kit is suitable for the isolation of small RNA transcripts larger but difficult to detect mRNA transcripts. For all kits, gift cost per sample analyzed is very high.

Exosome precipitation from Plasma + Thrombin
EPStep-T	ImmunoStep	5 ml	EUR 336

ExoQuick Exosome RNA Column Purification kit
EQ808A-1	SBI	20 preps	EUR 402

Exosome precipitation from Plasma and Serum
EPStep-PS	ImmunoStep	5 ml	EUR 297

ExoQuick-LP for lipoprotein pre-clear & exosome isolation
EXOLP5A-1	SBI	5 reactions	EUR 533
Category: Exosomes

PEG-it Virus Precipitation Solution (250 mL aliquot)
LV825A-1	SBI	250 ml	EUR 777
Category: Lentiviral Technology

PEG-it Virus Precipitation Solution (100 mL aliquot)
LV810A-1	SBI	100 ml	EUR 382
Category: Lentiviral Technology

ExoQuick-CG cGMP-grade Exosome Isolation reagent (50 ml)
EXOCG50A-1	SBI	50 ml	EUR 1181
Category: Exosomes

ExoQuick PLUS Exosome Purification Kit - for serum and plasma
EQPL10A-1	SBI	10 reactions	EUR 579
Category: Exosomes

ExoQuick-TC PLUS Exosome Purification Kit - for tissue culture media
EQPL10TC-1	SBI	10 reactions	EUR 579
Category: Exosomes

NATtrol Zika Virus Stock (Qualitative) (1 mL)
TEST	Zeptometrix	1 mL	EUR 1106.64
What is the product classification? NATtrol Zika Virus Stock Qualitative is marked as RUO.
Description: Please contact Gentaur in order to receive the datasheet of the product.

ExoQuick Exosome Isolation and RNA Purification kit (for Serum and Plasma)
EQ806A-1	SBI	20 preps	EUR 554

ExoQuick Exosome Isolation and RNA Purification kit (for Tissue Culture Media)
EQ806TC-1	SBI	10 preps	EUR 554

SeraMir Exosome RNA Purification kit (5ml ExoQuick and 20 exoRNA columns)
RA806A-1	SBI	20 preps	EUR 554
Category: MicroRNA Tools

Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL)
TMEXO-1	SBI	100 reactions	EUR 583
Category: Exosomes

Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome)
P101EZ	101Bio	NULL	EUR 0

ExoQuick TC 10ml
EXOTC10A-1	SBI	10 ml	EUR 399
Category: Exosomes

ExoQuick TC 50ml
EXOTC50A-1	SBI	50 ml	EUR 1222
Category: Exosomes

TMB for Precipitation
42R-TB100x	Fitzgerald	1000 ml	EUR 538
Description: High sensitivity TMB Substrate for Precipitation assays

DNA Precipitation Buffer
D106	Cygnus Technologies	55 ml	EUR 175
Product category: DNA and RNA extraction and purification
Description: DNA Precipitation Buffer by Cygnus Technologies is available in Europe via Gentaur.

Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome)
P100EZ	101Bio	NULL	EUR 0

Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome) sample
P101EZ--sample	101Bio	NULL	EUR 0

Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome) sample
P100EZ-sample	101Bio	NULL	EUR 0

SeraMir Exosome RNA Purification kit (10ml ExoQuick-TC and 10 exoRNA columns) 10 preps
RA806TC-1	SBI	10 preps	EUR 554
Category: MicroRNA Tools

Chicken IgY precipitation reagent
85R-1010	Fitzgerald	4 ml	EUR 554
Description: Chicken IgY precipitation reagent for use in immunoassays

PEG Virus Precipitation Kit
K904-200	Biovision		EUR 1001

PEG Virus Precipitation Kit
K904-50	Biovision		EUR 376

PEG Virus Precipitation Kit
K2190-200	ApexBio	200 preparations	EUR 1059

PEG Virus Precipitation Kit
K2190-50	ApexBio	50 preparations	EUR 405

50ML Protein Precipitation Kit
NAT1254	National Diagnostics	50ML	EUR 225

SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns)
RA800TC-1	SBI	10 reactions	EUR 659
Category: MicroRNA Tools

101Bio High Efficiency Protein Precipitation kit
P5W6	101Bio	NULL	EUR 0

In Vitro SDS-K+ Precipitation Kit
TG1010-1	TopoGen	100 assays	EUR 459

In Vitro SDS-K+ Precipitation Kit
TG1010-2	TopoGen	250 assays	EUR 571

In Vivo SDS-K+ Precipitation Kit
TG1011-1	TopoGen	100 assays	EUR 302

In Vivo SDS-K+ Precipitation Kit
TG1011-2	TopoGen	250 assays	EUR 414

Accu-Tell COVID-19 IgG/IgM Rapid Test
GEN-B352-20tests	Accu test	20 tests	EUR 236
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

Accu-Tell COVID-19 IgG/IgM Rapid Test
GEN-B352-40tests	Accu test	40 tests	EUR 321
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

Psoriasis Exosome
P141-PS	101Bio	NULL	EUR 0

DNASE TEST AGAR
D04-101-10kg	Alphabiosciences	10 kg	Ask for price

DNASE TEST AGAR
D04-101-2Kg	Alphabiosciences	2 Kg	Ask for price

DNASE TEST AGAR
D04-101-500g	Alphabiosciences	500 g	Ask for price

DERMATOPHYTE TEST AGAR
D04-107-10kg	Alphabiosciences	10 kg	EUR 849

DERMATOPHYTE TEST AGAR
D04-107-2Kg	Alphabiosciences	2 Kg	EUR 224

DERMATOPHYTE TEST AGAR
D04-107-500g	Alphabiosciences	500 g	EUR 97

MOTILITY TEST MEDIUM
M13-121-10kg	Alphabiosciences	10 kg	EUR 1528

MOTILITY TEST MEDIUM
M13-121-2Kg	Alphabiosciences	2 Kg	EUR 371

MOTILITY TEST MEDIUM
M13-121-500g	Alphabiosciences	500 g	EUR 137

SNYDER TEST AGAR
S19-119-10kg	Alphabiosciences	10 kg	EUR 1049

SNYDER TEST AGAR
S19-119-2Kg	Alphabiosciences	2 Kg	EUR 267

SNYDER TEST AGAR
S19-119-500g	Alphabiosciences	500 g	EUR 109

SAM Test Strip
TS00201s-30	Arthus	30 tests	EUR 416
Description: S-adenosylmethionine quantitative test strip for serum, plasma and whole blood

SAH Test Strip
TS00301s-30	Arthus	30 tests	EUR 504
Description: S-adenosylhomocysteine quantitative test strip for serum, plasma and whole blood

HCY Test Strip
TS00401s-30	Arthus	30 tests	EUR 553
Description: Serum homocysteine quantitative test strip

Lyophilized Exosome Standards
ExoA375	ImmunoStep	100 μg	EUR 303.5

Lyophilized Exosome Standards
ExoHT29	ImmunoStep	100 μg	EUR 303.5

Lyophilized Exosome Standards
ExoMCF7	ImmunoStep	100 μg	EUR 303.5

Lyophilized Exosome Standards
ExoPC3	ImmunoStep	100 μg	EUR 303.5

Lyophilized Exosome Standards
ExoPLASMA	ImmunoStep	100 μg	EUR 303.5

Lyophilized Exosome Standards
ExoUrine	ImmunoStep	100 μg	EUR 303.5

Leukemia - ALL Exosome
P141-ALL	101Bio	NULL	EUR 0

Leukemia - AML Exosome
P141-AML	101Bio	NULL	EUR 0

Bladder Cancer Exosome
P141-BL	101Bio	NULL	EUR 0

Breast Cancer Exosome
P141-BR	101Bio	NULL	EUR 0

Leukemia - CLL Exosome
P141-CLL	101Bio	NULL	EUR 0

Leukemia - CML Exosome
P141-CML	101Bio	NULL	EUR 0

Colorectal Cancer Exosome
P141-CO	101Bio	NULL	EUR 0

Crohn's Disease Exosome
P141-CR	101Bio	NULL	EUR 0

Kidney Cancer Exosome
P141-KN	101Bio	NULL	EUR 0

Lung Cancer Exosome
P141-LG	101Bio	NULL	EUR 0

Liver Cancer Exosome
P141-LV	101Bio	NULL	EUR 0

Nasopharyngeal Cancer Exosome
P141-NA	101Bio	NULL	EUR 0

Prostate Cancer Exosome
P141-PR	101Bio	NULL	EUR 0

Rheumatoid Arthritis Exosome
P141-RA	101Bio	NULL	EUR 0

Thyroid Cancer Exosome
P141-TH	101Bio	NULL	EUR 0

Psoriasis Exosome RNA
P241-PS	101Bio	NULL	EUR 0

2019-nCoV IgG/IgM Rapid Test Cassette (Whole Blood/Serum/Plasma)
GEN-402-25tests	All test	25 tests	EUR 244
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

Sodium Pyruvate Solution, 100 mM Solution
CCM1753-100	Bio Basic	100 mL	EUR 60.84
Product category: Culture Media

SDS Solution
2102-100	Biovision		EUR 137

EDTA Solution
2103-100	Biovision		EUR 137

TRIMMING SOLUTION
99-676-CM	CORNING	1 L /pk	EUR 88
Description: Specialty Media; Islet products

AlphaBioCoat Solution
AC001	Neuromics	20 ml	EUR 271

Borax Solution
BOR015	ScyTek Laboratories	15 ml	EUR 56

Borax Solution
BOR125	ScyTek Laboratories	125 ml	EUR 79

Borax Solution
BOR500	ScyTek Laboratories	500 ml	EUR 119

Borax Solution
BOR999	ScyTek Laboratories	1000 ml	EUR 183

Stop Solution
F006	Cygnus Technologies	12 ml	EUR 180
Product category: Buffer solution
Description: Stop Solution by Cygnus Technologies is available in Europe via Gentaur.

Prehybridization Solution
K2191050-1	Biochain	5 ml	EUR 137
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Hybridization Solution
K2191050-2	Biochain	5 ml	EUR 157
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Blocking Solution
K2191050-8	Biochain	250 ul	EUR 137
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Orcein Solution
OAA125	ScyTek Laboratories	125 ml	EUR 66

Orcein Solution
OAA500	ScyTek Laboratories	500 ml	EUR 109

Orcein Solution
OAA999	ScyTek Laboratories	1000 ml	EUR 148

Methenamine Solution
MET125	ScyTek Laboratories	125 ml	EUR 64

Methenamine Solution
MET500	ScyTek Laboratories	500 ml	EUR 95

Methenamine Solution
MET999	ScyTek Laboratories	1000 ml	EUR 132

DTT Solution
R-1104	EpiGentek	1 ml, 500 mM	EUR 109.45
Description: fast delivery possible

Tartrazine Solution
TZQ125	ScyTek Laboratories	125 ml	EUR 64

Tartrazine Solution
TZQ500	ScyTek Laboratories	500 ml	EUR 98

Tartrazine Solution
TZQ999	ScyTek Laboratories	1000 ml	EUR 127

Schiff's Solution
SRF030	ScyTek Laboratories	30 ml	EUR 64

Schiff's Solution
SRF250	ScyTek Laboratories	250 ml	EUR 77

Schiff's Solution
SRF3800	ScyTek Laboratories	1 Gal.	EUR 345

Schiff's Solution
SRF500	ScyTek Laboratories	500 ml	EUR 103

Schiff's Solution
SRF999	ScyTek Laboratories	1000 ml	EUR 149

Denaturing solution
DE540	Bio Basic	1L	EUR 61.31
Product category: Electrophoresis Related/Reagents

Exosome Isolation kit (for serum & plasma -Exosome-RNA-NGS)
P101NGS	101Bio	NULL	EUR 0

50ml TC Tubes, Conical, 440 units/box
04-5540150	Gentaur Genprice	440 units/box	EUR 71

GMP IL4, 50µg
04-GMP-HU-IL4-50UG	Gentaur Genprice	50µg	EUR 483
Description: Recombinant Human IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15000 Dalton. The rHuIL-4 is purified by proprietary chromatographic techniques.

rHu IL 2 , 3MIU
04-RHIL2-02F01	Gentaur Genprice	1 vial	EUR 249
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated.

rHu IL 2 , 3MIU , Lot 200908F02
04-RHIL2-08F02	Gentaur Genprice	1 vial	EUR 249
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated.

PRE-GMP rHu GM-CSF, Molgramostim-Leukoma
04-RHUGM-CSF-7A10	Gentaur Genprice	300 µg	EUR 385
Description: Recombinant human GM-CSF produced in E.coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids, two pairs of disulfide bonds and having a molecular mass of approximately 14.5kD.

Gentamicin Solution, Gentamicin Solution, 50 mg/mL
CCM1123-010	Bio Basic	10 mL	EUR 64.05
Product category: Culture Media

CA72-4 ELISA test
24	Biobase	96T/Box	Ask for price
Area of application: Hormone testing
Description: ELISA based test for quantitative detection of CA72-4

FERR ( Ferritin) ELISA test
7	Biobase	96T/Box	Ask for price
Area of application: Hormone testing
Description: ELISA based test for quantitative detection of FERR ( Ferritin)

TSH (Thyrotrophin) ELISA test
14	Biobase	96T/Box	Ask for price
Area of application: Hormone testing
Description: ELISA based test for quantitative detection of TSH (Thyrotrophin)

T3 (Triiodothyronine) ELISA test
15	Biobase	96T/Box	Ask for price
Area of application: Hormone testing
Description: ELISA based test for quantitative detection of T3 (Triiodothyronine)

T4 (Thyroxine) ELISA test
16	Biobase	96T/Box	Ask for price
Area of application: Hormone testing
Description: ELISA based test for quantitative detection of T4 (Thyroxine)

TESTO (Testosterone) ELISA test
19	Biobase	96T/Box	Ask for price
Area of application: Hormone testing
Description: ELISA based test for quantitative detection of O (osterone)

PROG (Progesterone) ELISA test
20	Biobase	96T/Box	Ask for price
Area of application: Hormone testing
Description: ELISA based test for quantitative detection of PROG (Progesterone)

E2 (Estradiol) ELISA test
5	Biobase	96T/Box	Ask for price
Area of application: Hormone testing
Description: ELISA based test for quantitative detection of E2 (Estradiol)

PRL (Prolactin) ELISA test
4	Biobase	96T/Box	Ask for price
Area of application: Hormone testing
Description: ELISA based test for quantitative detection of PRL (Prolactin)

Basic Cytotoxicity Test Kits
CT17001	Neuromics	125 Tests	EUR 409

Basic Cytotoxicity Test Kits
CT17002	Neuromics	250 Tests	EUR 650

Total Cytotoxicity Test Kits
CT17003	Neuromics	125 Tests	EUR 513

Total Cytotoxicity Test Kits
CT17004	Neuromics	250 Tests	EUR 813

Clearview Chlamydia MF test
CV504524	Bionote	1 box	EUR 162
Description: Please check the datasheet of Clearview Chlamydia MF test before using the test.

PreS2-Ag ELISA test
96	Biobase	96T/Box	Ask for price
Area of application: Hepatitis testing
Description: ELISA based test for quantitative detection of PreS2-Ag

HBcAb Sandwich ELISA test
95	Biobase	96T/Box	Ask for price
Area of application: Hepatitis testing
Description: ELISA based test for quantitative detection of HBcAb Sandwich

Melamine Rapid Test Kit
abx092011-50tests	Abbexa	50 tests	EUR 370
Shipped within 5-12 working days.

NDV rapid test kit
RG15-03	Bionote	1 box	EUR 139.05
Description: Please check the datasheet of NDV rapid test kit before using the test.

IBD rapid test strip
RG15-04	Bionote	10 boxes	EUR 148
Description: Please check the datasheet of IBD rapid test strip before using the test.

Rabies rapid test strip
RG18-01	Bionote	10 boxes	EUR 151.92
Description: Please check the datasheet of Rabies rapid test strip before using the test.

ExoQuick ULTRA EV Isolation Kit for Serum and Plasma
EQULTRA-20A-1	SBI	20 reactions	EUR 579

Exosome Component 5 Protein
20-abx260873	Abbexa	EUR 3418.00 EUR 328.00 EUR 230.00	1 mg 25 ug 5 ug
Shipped within 5-10 working days.

Exosome Component 7 Protein
20-abx261347	Abbexa	EUR 3418.00 EUR 328.00 EUR 230.00	1 mg 20 ug 5 ug
Shipped within 5-10 working days.

Exosome Component 10 Protein
20-abx261805	Abbexa	EUR 4365.00 EUR 328.00 EUR 230.00	1 mg 20 ug 5 ug
Shipped within 5-10 working days.

Exosome Component 4 Protein
20-abx261954	Abbexa	EUR 4490.00 EUR 328.00 EUR 230.00	1 mg 20 ug 5 ug
Shipped within 5-10 working days.

Exosome Component 9 Protein
20-abx262123	Abbexa	EUR 328.00 EUR 5089.00 EUR 230.00	10 ug 1 mg 2 µg
Shipped within 5-10 working days.

Exosome Component 3 Protein
20-abx263422	Abbexa	EUR 230.00 EUR 1609.00 EUR 328.00	1 µg 50 ug 5 ug
Shipped within 5-10 working days.

Exosome Component 1 Protein
20-abx263423	Abbexa	EUR 1609.00 EUR 328.00 EUR 230.00	100 ug 10 ug 2 µg
Shipped within 5-10 working days.

Exosome Component 8 Protein
20-abx262850	Abbexa	EUR 328.00 EUR 6397.00 EUR 230.00	10 ug 1 mg 2 µg
Shipped within 5-10 working days.

EXOCET Exosome Quantitation Kit
EXOCET96A-1	SBI	96 reactions	EUR 521
Category: Exosomes

FluoroCet Exosome Quantitation Kit
FCET96A-1	SBI	96 reactions	EUR 726
Category: Exosomes

Fluorescent Exosome Standard (Urine )
ESF-15	Creative Biolabs	100 µg	EUR 1723
Description: Fluorescent exosomes are labeled with green dye, which offer a stable fluorescent labeling.

Exosome-TEM-easy Kit
P130	101Bio	NULL	EUR 0

Diabetes (Type 1) Exosome
P141-DB1	101Bio	NULL	EUR 0

Diabetes (Type 2) Exosome
P141-DB2	101Bio	NULL	EUR 0

Leukemia - Hairy Cell Exosome
P141-HC	101Bio	NULL	EUR 0

Leukemia - Multiple Myeloma Exosome
P141-MM	101Bio	NULL	EUR 0

Melanoma (skin cancer) Exosome
P141-MN	101Bio	NULL	EUR 0

Non-Hodgkin lymphoma Exosome
P141-NH	101Bio	NULL	EUR 0

Systemic Lupus Erythematosus Exosome
P141-SL	101Bio	NULL	EUR 0

Our current method provides a new way for the direct conversion of exosomes into cDNA synthesis (Exo-cDNA) and subsequent gene detection by the polymerase chain reaction (PCR). This method has several advantages over the established kit available. There is no extraction column is used in this procedure, which means a total recovery of RNA exosomal with maximum results.