Circular RNAs expression profiles in plasma exosomes from early-stage lung adenocarcinoma and the potential biomarkers.

This study aims to identify the circular RNA differential (circRNA) in exosomes plasma of patients with lung adenocarcinoma (LUAD) using high-throughput sequencing. First, exosomes were isolated using a kit exosome isolation and confirmed by Western blotting, transmission electron microscopy, and NanoSight Assay. Furthermore, the expression profile circRNA plasma is filtered by high-throughput sequencing and confirmed by fluorescence quantitative real-time polymerase chain reaction (qRT-PCR) and sequencing Sanger.

Finally, network-miRNA-mRNA circRNA performed to predict the potential function circRNAs. The results of the high-throughput sequencing data documented that exosomal circRNAs 182 differentially expressed in all screened, including 105 upregulated and 78 were downregulated in plasma LUAD patients compared with controls. Fourth circRNAs regulated including circ_0001492, circ_0001346, circ_0000690, and circ_0001439 synonymous with sequencing data by qRT-PCR, and the latent interaction circRNA-miRNA-mRNA were exhibited. Taken together, our studies first revealed altered expression exosomal circRNA from plasma samples in patients with LUAD and support the need to explore their potential as biomarkers and pathological effects of lung cancer.


Exosomes are vesicles with sizes ranging from 30-150 nm. Analysis and detection of blood exosomes offer an effective service for cancer diagnosis, prognosis assessment, and evaluation of disease therapies. Because of the difference in separation procedures, methods of collection and use of anticoagulants, serum and plasma samples showed the diversity of the test results.

In order to evaluate the effect of exosomes isolation in serum and plasma samples, two methods commonly used insulation exosomal, ultracentrifugation and precipitation kit based polymers, used, respectively. And the insulating effect was evaluated by comparing the composition and abundant proteins from exosomes isolated based on the analysis of MS-based proteomics. The results showed that plasma exosomes extracted by ultracentrifugation more exosome identify biomarkers and biomarker concentration is higher than others.

And plasma exosomes could be a good example for the blood-based proteomics study of exosomes. It would be useful for future targeted biomarker discovery. This article is protected by copyright. All rights reserved.

 Circular RNAs expression profiles in plasma exosomes from early-stage lung adenocarcinoma and the potential biomarkers.
Circular RNAs expression profiles in plasma exosomes from early-stage lung adenocarcinoma and the potential biomarkers.

Exosomes molecular diagnostics: direct conversion of exosomes into cDNA for gene amplification by polymerase chain reaction two steps.

Exosomes are cells derived lipid nanoparticles with a size of 30-100 nm in diameter, was found in almost all biological fluids. The composition of exosomes mainly lipids, proteins, RNA, DNA, and non-coding RNA. At present, most of the available methods and commercial kits for exosomal RNA (Exo-RNA) isolation has its limitations and shortcomings.

Starting small volume of exosomes and the use of extraction / filtration column is usually not enough outcome results exosomal RNA after isolation. The majority of RNA contained in exosomes purified various sizes 15-500 nucleotides. Some RNA isolation kit is suitable for the isolation of small RNA transcripts larger but difficult to detect mRNA transcripts. For all kits, gift cost per sample analyzed is very high.

ExoQuick-LP for lipoprotein pre-clear & exosome isolation

EXOLP5A-1 5 reactions
EUR 639.6

Porcine Parvovirus Antibody Elisa Test Kit

767-LSY-30009 192 Wells/kit
EUR 382

ExoQuick PLUS Exosome Purification Kit - for serum and plasma

EQPL10A-1 10 reactions
EUR 694.8

ExoQuick-CG cGMP-grade Exosome Isolation reagent (50 ml)

EXOCG50A-1 50 ml
EUR 1417.2

PEG-it Virus Precipitation Solution (100 mL aliquot)

LV810A-1 100 ml
EUR 458.4

PEG-it Virus Precipitation Solution (250 mL aliquot)

LV825A-1 250 ml
EUR 932.4

ExoQuick-TC PLUS Exosome Purification Kit - for tissue culture media

EQPL10TC-1 10 reactions
EUR 694.8

NATtrol Zika Virus Stock (Qualitative) (1 mL)

TEST 1 mL
EUR 1327.97
Description: Please contact Gentaur in order to receive the datasheet of the product.

ExoQuick Exosome Isolation and RNA Purification kit (for Serum and Plasma)

EQ806A-1 20 preps
EUR 664.8

ExoQuick Exosome Isolation and RNA Purification kit (for Tissue Culture Media)

EQ806TC-1 10 preps
EUR 664.8

SeraMir Exosome RNA Purification kit (5ml ExoQuick and 20 exoRNA columns)

RA806A-1 20 preps
EUR 664.8

Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL)

TMEXO-1 100 reactions
EUR 699.6

SDS-Blue™ - Coomassie based solution for protein staining in SDS-PAGE

04-GSB
  • EUR 144.00
  • EUR 96.00
  • 1L
  • 500mL
Description: SDS-Blue™ is an innovative patented formula, based on Coomassie blue, that comes in a convenient ready to use format for staining proteins in SDS-PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis). The formulation of SDS-Blue™ provides numerous advantages compared to the classic Coomassie staining or to other similar protein stains. SDS-Blue™ provides higher sensitivity, virtually no background and eliminates the need for destaining of the gel due to its high specificity and affinity to bind to protein only. Not only does SDS-Blue™ yield clear and sharp bands, but it also contains no methanol and acetic acid, making it non-hazardous, safe to handle and friendly to the environment when disposed of. Two other advantages that make SDS-Blue™ the better option is that it is not light sensitive and can be stored at ambient temperature for 24 months. And this provides a considerable convenience, especially to laboratories that need and keep big amount of protein staining solutions – no more jammed refrigerators, you can keep SDS-Blue™ wherever it is most convenient for You!

Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome)

P101EZ - Ask for price

ExoQuick TC 10ml

EXOTC10A-1 10 ml
EUR 478.8

ExoQuick TC 50ml

EXOTC50A-1 50 ml
EUR 1466.4

TMB for Precipitation

42R-TB100x 1000 ml
EUR 645.6
Description: High sensitivity TMB Substrate for Precipitation assays

DNA Precipitation Buffer

D106 55 ml
EUR 210
Description: DNA Precipitation Buffer by Cygnus Technologies is available in Europe via Gentaur.

Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome)

P100EZ - Ask for price

Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome) sample

P101EZ--sample - Ask for price

Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome) sample

P100EZ-sample - Ask for price

SeraMir Exosome RNA Purification kit (10ml ExoQuick-TC and 10 exoRNA columns) 10 preps

RA806TC-1 10 preps
EUR 664.8

Chicken IgY precipitation reagent

85R-1010 4 ml
EUR 664.8
Description: Chicken IgY precipitation reagent for use in immunoassays

PEG Virus Precipitation Kit

K904-200
EUR 1201.2

PEG Virus Precipitation Kit

K904-50
EUR 451.2

PEG Virus Precipitation Kit

K2190-200 200 preparations
EUR 1270.8

PEG Virus Precipitation Kit

K2190-50 50 preparations
EUR 486

50ML Protein Precipitation Kit

NAT1254 50ML
EUR 237.12

SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns)

RA800TC-1 10 reactions
EUR 790.8

Psoriasis Exosome

P141-PS - Ask for price

101Bio High Efficiency Protein Precipitation kit

P5W6 - Ask for price

In Vitro SDS-K+ Precipitation Kit

TG1010-1 100 assays
EUR 550.8

In Vitro SDS-K+ Precipitation Kit

TG1010-2 250 assays
EUR 685.2

In Vivo SDS-K+ Precipitation Kit

TG1011-1 100 assays
EUR 362.4

In Vivo SDS-K+ Precipitation Kit

TG1011-2 250 assays
EUR 496.8

Kinesis TELOS PPT Protein Precipitation Plate

CHR6509 EACH
EUR 84.3

Kinesis TELOS PPT Protein Precipitation Columns

CHR1378 PK100
EUR 190.41

Accu-Tell COVID-19 IgG/IgM Rapid Test

GEN-B352-20tests 20 tests
EUR 283.2
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

Accu-Tell COVID-19 IgG/IgM Rapid Test

GEN-B352-40tests 40 tests
EUR 385.2
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

Solution

S-2140-A 1ML
EUR 69.54

Test product

testtt 0 Ask for price

Leukemia - ALL Exosome

P141-ALL - Ask for price

Leukemia - AML Exosome

P141-AML - Ask for price

Bladder Cancer Exosome

P141-BL - Ask for price

Breast Cancer Exosome

P141-BR - Ask for price

Leukemia - CLL Exosome

P141-CLL - Ask for price

Leukemia - CML Exosome

P141-CML - Ask for price

Colorectal Cancer Exosome

P141-CO - Ask for price

Crohn's Disease Exosome

P141-CR - Ask for price

Kidney Cancer Exosome

P141-KN - Ask for price

Lung Cancer Exosome

P141-LG - Ask for price

Liver Cancer Exosome

P141-LV - Ask for price

Nasopharyngeal Cancer Exosome

P141-NA - Ask for price

Prostate Cancer Exosome

P141-PR - Ask for price

Rheumatoid Arthritis Exosome

P141-RA - Ask for price

Thyroid Cancer Exosome

P141-TH - Ask for price

Lyophilized Exosome Standards

ExoA375 100 μg
EUR 364.2

Lyophilized Exosome Standards

ExoHT29 100 μg
EUR 364.2

Lyophilized Exosome Standards

ExoMCF7 100 μg
EUR 364.2

Lyophilized Exosome Standards

ExoPC3 100 μg
EUR 364.2

Lyophilized Exosome Standards

ExoPLASMA 100 μg
EUR 364.2

Lyophilized Exosome Standards

ExoUrine 100 μg
EUR 364.2

Psoriasis Exosome RNA

P241-PS - Ask for price

Exosome Isolation kit (for serum & plasma -Exosome-RNA-NGS)

P101NGS - Ask for price

2019-nCoV IgG/IgM Rapid Test Cassette (Whole Blood/Serum/Plasma)

GEN-402-25tests 25 tests
EUR 292.8
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

Sodium Pyruvate Solution, 100 mM Solution

CCM1753-100 100 mL
EUR 73.01

Membrane Protein Extraction Solution (Solution #1)

MPE01 40 ml
EUR 167.04
Description: This product includes 40 ml of Solution #1 for large-scale preparative extraction of membrane proteins (up to 130 mg) from the cell membranes.

Membrane Protein Extraction Solution (Solution #2)

MPE02 40 ml
EUR 167.04
Description: This product includes 40 ml of Solution #2 for large-scale preparative extraction of membrane proteins (up to 130 mg) from the cell membranes.

Membrane Protein Extraction Solution (Solution #3)

MPE03 40 ml
EUR 167.04
Description: This product includes 40 ml of Solution #3 for large-scale preparative extraction of membrane proteins (up to 130 mg) from the cell membranes.

Membrane Protein Extraction Solution (Solution #4)

MPE04 40 ml
EUR 167.04
Description: This product includes 40 ml of Solution #4 for large-scale preparative extraction of membrane proteins (up to 130 mg) from the cell membranes.

Membrane Protein Extraction Solution (Solution #5)

MPE05 40 ml
EUR 167.04
Description: This product includes 40 ml of Solution #5 for large-scale preparative extraction of membrane proteins (up to 130 mg) from the cell membranes.

Membrane Protein Extraction Solution (Solution #6)

MPE06 40 ml
EUR 167.04
Description: This product includes 40 ml of Solution #6 for large-scale preparative extraction of membrane proteins (up to 130 mg) from the cell membranes.

Membrane Protein Extraction Solution (Solution #7)

MPE07 40 ml
EUR 167.04
Description: This product includes 40 ml of Solution #7 for large-scale preparative extraction of membrane proteins (up to 130 mg) from the cell membranes.

Membrane Protein Extraction Solution (Solution #8)

MPE08 40 ml
EUR 167.04
Description: This product includes 40 ml of Solution #8 for large-scale preparative extraction of membrane proteins (up to 130 mg) from the cell membranes.

Membrane Protein Extraction Solution (Solution #9)

MPE09 40 ml
EUR 167.04
Description: This product includes 40 ml of Solution #9 for large-scale preparative extraction of membrane proteins (up to 130 mg) from the cell membranes.

Membrane Protein Extraction Solution (Solution #10)

MPE10 40 ml
EUR 167.04
Description: This product includes 40 ml of Solution #10 for large-scale preparative extraction of membrane proteins (up to 130 mg) from the cell membranes.

Membrane Protein Extraction Solution (Solution #11)

MPE11 40 ml
EUR 167.04
Description: This product includes 40 ml of Solution #11 for large-scale preparative extraction of membrane proteins (up to 130 mg) from the cell membranes.

Membrane Protein Extraction Solution (Solution #12)

MPE12 40 ml
EUR 167.04
Description: This product includes 40 ml of Solution #12 for large-scale preparative extraction of membrane proteins (up to 130 mg) from the cell membranes.

Bovine Anti-Chicken IgY Beads for immuno-precipitation

XG-6134 1 mL
EUR 546.72
Description: The anti-IgY beads were made by cross-linking of bovine IgG anti-IgY antibodies to Protein A/G agarose beads. IgY is the original designation of chicken IgG like immunoglobulin.

SDS Solution

2102-100
EUR 164.4

EDTA Solution

2103-100
EUR 164.4

TRIMMING SOLUTION

99-676-CM 1 L /pk
EUR 105.6
Description: Specialty Media; Islet products

Denaturing solution

DE540 1L
EUR 73.57

Borax Solution

BOR015 15 ml
EUR 67.2

Borax Solution

BOR125 125 ml
EUR 94.8

Borax Solution

BOR500 500 ml
EUR 142.8

Borax Solution

BOR999 1000 ml
EUR 219.6

Methenamine Solution

MET125 125 ml
EUR 76.8

Methenamine Solution

MET500 500 ml
EUR 114

Methenamine Solution

MET999 1000 ml
EUR 158.4

Orcein Solution

OAA125 125 ml
EUR 79.2

Orcein Solution

OAA500 500 ml
EUR 130.8

Orcein Solution

OAA999 1000 ml
EUR 177.6

Stop Solution

F006 12 ml
EUR 216
Description: Stop Solution by Cygnus Technologies is available in Europe via Gentaur.

Plug Solution

GR103038 500 mL
EUR 102

Prehybridization Solution

K2191050-1 5 ml
EUR 164.4
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Hybridization Solution

K2191050-2 5 ml
EUR 188.4
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Blocking Solution

K2191050-8 250 ul
EUR 164.4
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Schiff's Solution

SRF030 30 ml
EUR 76.8

Schiff's Solution

SRF250 250 ml
EUR 92.4

Schiff's Solution

SRF3800 1 Gal.
EUR 414

Schiff's Solution

SRF500 500 ml
EUR 123.6

Schiff's Solution

SRF999 1000 ml
EUR 178.8

Tartrazine Solution

TZQ125 125 ml
EUR 76.8

Tartrazine Solution

TZQ500 500 ml
EUR 117.6

Tartrazine Solution

TZQ999 1000 ml
EUR 152.4

Fortification Solution

5242-F 1ML
EUR 36.48

Fortification Solution

5252-FS 1ML
EUR 42.18

Decalcifying Solution

DECAL01 100ML
EUR 31.92

TISAB Solution

TISAB1L-500ML 500ML
EUR 33.06

TISAB Solution

TISAB5 5L
EUR 191.52

Chloroprene Solution

S-930A 1ML
EUR 128.82

DTT Solution 

R-1104-1 1 ml, 500 mM
EUR 48.4

DTT Solution 

R-1104
  • EUR 131.34
  • EUR 48.40
  • 1 ml, 500 mM
  • 1 ml, 500 mM

DNASE TEST AGAR

D04-101-10kg 10 kg Ask for price

DNASE TEST AGAR

D04-101-2Kg 2 Kg Ask for price

DNASE TEST AGAR

D04-101-500g 500 g Ask for price

DERMATOPHYTE TEST AGAR

D04-107-10kg 10 kg
EUR 1018.8

DERMATOPHYTE TEST AGAR

D04-107-2Kg 2 Kg
EUR 268.8

DERMATOPHYTE TEST AGAR

D04-107-500g 500 g
EUR 116.4

MOTILITY TEST MEDIUM

M13-121-10kg 10 kg
EUR 1833.6

MOTILITY TEST MEDIUM

M13-121-2Kg 2 Kg
EUR 445.2

MOTILITY TEST MEDIUM

M13-121-500g 500 g
EUR 164.4

SNYDER TEST AGAR

S19-119-10kg 10 kg
EUR 1258.8

SNYDER TEST AGAR

S19-119-2Kg 2 Kg
EUR 320.4

SNYDER TEST AGAR

S19-119-500g 500 g
EUR 130.8

SAM Test Strip

TS00201s-30 30 tests
EUR 499.2
Description: S-adenosylmethionine quantitative test strip for serum, plasma and whole blood

SAH Test Strip

TS00301s-30 30 tests
EUR 604.8
Description: S-adenosylhomocysteine quantitative test strip for serum, plasma and whole blood

HCY Test Strip

TS00401s-30 30 tests
EUR 663.6
Description: Serum homocysteine quantitative test strip

Test Tube 16x100mm

3840/16X100 PK100
EUR 57.11

Dryslide Oxidase Test

231746 PK75
EUR 199.5

aa test product

123 1MG
EUR 279.6

Phenols Cuvette Test

LCK345 EACH
EUR 141.72

Dermatophyte Test Medium

MED1484 EACH
EUR 87.78

Test for Enteropathogenic.C

MIC6774 PK50
EUR 215.46

Sulphite Test Strips

MN91306 PK100
EUR 51.53

Thermal Test Film

PCR0890 PK25
EUR 383.04

5mL Test Tubes

TUB0228 PK1400
EUR 159.6

Spectroquant COD test

WAT1080 EACH
EUR 96.9

Phosphate Total Test

WAT3655 EACH
EUR 203.01

ExoQuick ULTRA EV Isolation Kit for Serum and Plasma

EQULTRA-20A-1 20 reactions
EUR 694.8

Exosome Component 9 Peptide

46-967P 0.1 mg
EUR 405.6
Description: (CT) PMSCL1 Peptide

Exosome Component 3 Protein

20-abx263422
  • EUR 276.00
  • EUR 1930.80
  • EUR 393.60
  • 1 µg
  • 50 ug
  • 5 ug

Exosome Component 1 Protein

20-abx263423
  • EUR 1930.80
  • EUR 393.60
  • EUR 276.00
  • 100 ug
  • 10 ug
  • 2 µg

Exosome Component 5 Protein

20-abx260873
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 25 ug
  • 5 ug

Exosome Component 7 Protein

20-abx261347
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

Exosome Component 10 Protein

20-abx261805
  • EUR 5238.00
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

Exosome Component 4 Protein

20-abx261954
  • EUR 5388.00
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

Exosome Component 9 Protein

20-abx262123
  • EUR 393.60
  • EUR 6106.80
  • EUR 276.00
  • 10 ug
  • 1 mg
  • 2 µg

Exosome Component 8 Protein

20-abx262850
  • EUR 393.60
  • EUR 7676.40
  • EUR 276.00
  • 10 ug
  • 1 mg
  • 2 µg

Exosome-TEM-easy Kit

P130 - Ask for price

Diabetes (Type 1) Exosome

P141-DB1 - Ask for price

Our current method provides a new way for the direct conversion of exosomes into cDNA synthesis (Exo-cDNA) and subsequent gene detection by the polymerase chain reaction (PCR). This method has several advantages over the established kit available. There is no extraction column is used in this procedure, which means a total recovery of RNA exosomal with maximum results.