Development of a CD63 Aptamer for Efficient Cancer Immunochemistry and Immunoaffinity-Based Exosome Isolation

CD63, members of Transmembrane-4-Superfamili Tetraspanin proteins and very n-glycosposomal membrane membrane proteins are known to regulate the malignancy of various types of cancer such as melanoma and breast cancer and function as potential markers for cancer detection. Recently, an important role as a classic exosome marker was also emphasized. In this work, through using a competitive Selex based magnetic beads (systematic evolution of ligands with exponential enrichment) and introducing NaCl 0.5m as an elution buffer, we identify two DNA aptamers (CD63-1 and CD63-2) with high affinity. and specificity for CD63 protein (KD = 38.71nm and 78.43, respectively).

Furthermore, CD63-1 was found efficiently in binding CD63 positive cells, including MDA-MB-231 cell breast cancer and too much HEK293T cells are expressed, with intermediate fastener affinity (KD ~ 100 Nm) as assessed by Flow cytometry. When the immunostaining test was carried out using clinical breast cancer biopsy, the CD63-1 APTIMER showed a comparable diagnostic efficacy for CD63 positive breast cancer with commercial antibodies. After developing the immunoaffinity imunoaffinity beads-based immunoaffinity system using Aptamer CD63-1, it was found that this bead-based system could effectively isolate the exosomes of MDA-MB-231 cell culture media and HT29.

Importantly, the introduction of Elution NaCl in this work allows the original exosom isolation through a simple 0.5m NaCl incubation step. Based on this result, we strongly believe that the aptamers developed can be useful for the exosom insulation of the original state efficiently from clinical samples and various terorostic applications for positive CD63 cancer.

Deposition of peptide affinity from extracellular vesicles and cell-free DNA improve sequencing performance for pathogenic mutation detection in plasma lung cancer patients

Liquid biopsy is a minimal-invasive diagnostic method that can increase access to molecular profiles for non-small patients lung cancer (NSCLC). Although the insulation of DNA (CF-DNA) cells from plasma is a standard liquid biopsy method to detect DNA mutations in cancer patients, its sensitivity can vary greatly. VN96 is a peptide with affinity for extracellular vesicles (EV) and circulating CF-DNA. In this study, we evaluated whether the Peptide-Affinity (PA) of EVS and CF-DNA precipitation of Plasma NSCLC patients increased the sensitivity of a single nucleotide variant (SNV) and compared with the observed SNV with those reported in suitable network biopsy.

Pope NSCLC patients are the target of PA precipitation or cell-free methods and total nucleic acids (TNA) are extracted; SNV is then detected by the next generation (NGS) sequencing. Pa causes an increase in DNA recovery and an increase in sequencing parameters of NGS when compared to CF-TNA. Reduced concordance with the network was observed in PA-TNA (62%) compared to CF-TNA (81%), mainly because of the identification of SNVs in Pa-TNA which were not observed in the network. The EGFR mutation was detected in PA-TNA with 83% sensitivity and 100% specificity.

In conclusion, PA-TNA can increase the abundant low allel detection limit using NGS. Stem Cell Educator Therapy (SCE) is a new clinical approach to the treatment of type 1 diabetes and other autoimmune diseases. SCE therapy circulates mononuclear blood cells isolated patients (for example, lymphocytes and monocytes) through apheresis machines, ko-culture mononuclear blood patients with stem cells that are reduced by the blood of adhered straps (CB-SC) on the SCE device, and then return ” Educated “these immune cells with patient blood. Exosom is a nano-sized extracellular vesicle between 30-150 nm in all biofluid media and cell culture.

 Development of a CD63 Aptamer for Efficient Cancer Immunochemistry and Immunoaffinity-Based Exosome Isolation
Development of a CD63 Aptamer for Efficient Cancer Immunochemistry and Immunoaffinity-Based Exosome Isolation

Clinical applications of extracellular vesicles of mesenchymal stem / stromal cells: therapeutic potential of an acellular product

Over the past decade, the sequery activity of mesenchymal stem cells (MSCS) has been widely studied due to its possible therapeutic role. In fact, MSCS releases extracellular vesicles (EVS) containing relevant biomolecules such as MRNAS, microarnas, bioactive lipids and signaling receptors, capable of restoring physiological conditions in which regenerative or anti-inflammatory actions are required. A real benefit would come from the therapeutic use of EV relative to MSC, avoiding possible immune release, pulmonary trapping, safety improvement and allowing crossing of organic barriers. A number of concerns must still be resolved concerning the mechanisms determining the beneficial effect of the MSC-EVS, the possible modification of their properties as a result of isolation / purification methods and / or the best approach for large production. scale for clinical use.

Most of the preclinical studies have been successful, reporting the role of MSC-EVS a role of protection in acute kidney injury after the opposing ischemia, powerful anti-inflammatory and anti-fibrotic effects by reducing inflammation Associated with diseases and fibrosis in lung and liver, as well as the modulation of innate and adaptive immune responses in transplant disease compared to host diseases (GVHD) as well as autoimmune diseases. However, the translation of MSC-EVS to the clinical stage is still in the initial phase. In this document, we discuss the therapeutic potential of an acellular product such as MSC derivative EVS (MSC-EVS) in acute and chronic pathologies.

Rapid Fungal Genomic DNA Isolation Kit

FT71415 50Preps
EUR 91.97

Easy3-Plant Genomic DNA Isolation Kit

FYG503-100P 100 Preps Ask for price

Easy3-Plant Genomic DNA Isolation Kit

FYG503-500P 500 Preps Ask for price

Plant Tissue Genomic DNA Isolation Kit

K316-100
EUR 451.2

One-Tube Bacteria Genomic DNA Isolation Kit

BS8413 100preps
EUR 94.72

One-Tube Bacteria Genomic DNA Isolation Kit

BS8414 500Preps, 500prep
EUR 198.85

Genomic DNA Kit

20-abx098076
  • EUR 526.80
  • EUR 292.80
  • 200 rxns
  • 50 rxns

Mitochondrial DNA Isolation Kit

55R-1361 50 assays
EUR 990
Description: Mitochondrial DNA Isolation Kit for use in the research laboratory

Mitochondrial DNA Isolation Kit

K2117-50 50 assays
EUR 718.8

Saliva DNA Isolation Kit

K5011050 1 kit
EUR 307.2

Urine DNA Isolation Kit

K5011150 1 kit
EUR 307.2

Stool DNA Isolation Kit

K5011250 1 kit
EUR 307.2

Serum DNA Isolation Kit

K5018100 1 kit
EUR 432

Mitochondrial DNA Isolation Kit

K280-50
EUR 705.6

Plant Genomic DNA Kit

20-abx098077
  • EUR 493.20
  • EUR 276.00
  • 200 rxns
  • 50 rxns

Bacteria Genomic DNA Kit

20-abx098080
  • EUR 126.00
  • EUR 292.80
  • 2 rxns
  • 50 rxns

Micro Genomic DNA Kit

20-abx098242
  • EUR 126.00
  • EUR 393.60
  • 2 rxns
  • 50 rxns

Blood Genomic DNA Kit

abx098868-50rxns 50 rxns
EUR 326.4

Plant Genomic DNA Kit

abx294004-50preps 50 preps
EUR 477.6

Animal Genomic DNA Kit

abx294005-100preps 100 preps
EUR 627.6

Animal Genomic DNA Kit

abx294005-50preps 50 preps
EUR 477.6

Soil Genomic DNA Kit

K1411-250
EUR 673.2

Soil Genomic DNA Kit

K1411-50
EUR 379.2

Insect Genomic DNA Kit

K1412-250
EUR 673.2

Insect Genomic DNA Kit

K1412-50
EUR 379.2

Mollusc Genomic DNA Kit

K1413-250
EUR 673.2

Mollusc Genomic DNA Kit

K1413-50
EUR 379.2

Yeast Genomic DNA Kit

K1414-250
EUR 673.2

Yeast Genomic DNA Kit

K1414-50
EUR 379.2

Fungal Genomic DNA Kit

K1415-250
EUR 673.2

Fungal Genomic DNA Kit

K1415-50
EUR 379.2

Human Genomic DNA

BIO-35025 500µl @ 200ng/µl Ask for price

Human Genomic DNA 

X11000-1 0.2 ml
EUR 235.4

Human Genomic DNA 

X11000
  • Ask for price
  • EUR 235.40
  • 0.2 ml
  • 0.2 ml

Apoptotic DNA Ladder Isolation Kit

K170-50
EUR 561.6

Genoirse Plant DNA Isolation Kit

GR101083 50
EUR 267.6

Apoptotic DNA Ladder Isolation Kit

K2045-50 50 assays
EUR 602.4

Whole Blood DNA Isolation Kit

K528-100
EUR 451.2

XCF Exosomal DNA Isolation Kit

XCF200A-1 20 rxn
EUR 463.2

FitAmp Gel DNA Isolation Kit

P-1007 100 Samples
EUR 324.48

FitAmp Urine DNA Isolation Kit 

P-1017
  • EUR 474.12
  • EUR 171.60
  • EUR 292.60
  • 100 Samples
  • 50 Samples
  • 100 Samples

FitAmp Urine DNA Isolation Kit 

P-1017-050 50 Samples
EUR 171.6

FitAmp Urine DNA Isolation Kit 

P-1017-100 100 Samples
EUR 292.6

Marine Animal Genomic DNA Kit

20-abx098079
  • EUR 126.00
  • EUR 292.80
  • 2 rxns
  • 50 rxns

FFPE Tissue Genomic DNA Kit

abx098866-50rxns 50 rxns
EUR 326.4

ISOLATE II Genomic DNA Kit

BIO-52065 10 preps Ask for price

ISOLATE II Genomic DNA Kit

BIO-52066 50 preps Ask for price

ISOLATE II Genomic DNA Kit

BIO-52067 250 preps Ask for price

AccuRT Genomic DNA Removal Kit

G488 200 Reactions
EUR 98.4

Zenoquick Genomic DNA Purification Kit

Z2003-200 200 preps
EUR 757.2

Zenoprep Genomic DNA Purification Kit

Z2004-400 400 preps
EUR 660

Sera-Xtracta Genomic DNA Kit

29429140 EACH
EUR 380.76

Genomic DNA - Human Male

D1234999-G01 100 ug
EUR 225.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Human Female

D1234999-G02 100 ug
EUR 225.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Mouse Male

D1334999-G01 100 ug
EUR 225.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Mouse Female

D1334999-G02 100 ug
EUR 225.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rat Male

D1434999-G01 100 ug
EUR 225.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rat Female

D1434999-G02 100 ug
EUR 225.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Dog Male

D1734999-G01 100 ug
EUR 225.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Dog Female

D1734999-G02 100 ug
EUR 225.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rabbit Male

D1834999-G01 100 ug
EUR 225.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rabbit Female

D1834999-G02 100 ug
EUR 225.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Porcine Male

D1934999-G01 100 ug
EUR 225.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Porcine Female

D1934999-G02 100 ug
EUR 225.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Bovine Male

D1B34999-G01 100 ug
EUR 225.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Bovine Female

D1B34999-G02 100 ug
EUR 225.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Chicken Male

D1C34999-G01 100 ug
EUR 225.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Chicken Female

D1C34999-G02 100 ug
EUR 225.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Hamster Male

D1H34999-G01 100 ug
EUR 225.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Hamster Female

D1H34999-G02 100 ug
EUR 225.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Horse Male

D1O34999-G01 100 ug
EUR 225.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Horse Female

D1O34999-G02 100 ug
EUR 225.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Human Brain Genomic DNA  

X11001-1 10 ul
EUR 77

Human Brain Genomic DNA  

X11001
  • Ask for price
  • EUR 77.00
  • 10 µg
  • 10 ul

100 BP DNA LADDER, 500UL PER KIT

M-DNA-100BP 1/pk
EUR 87.6
Description: Bioscience Mol Bio; DNA Ladder

1 KB DNA LADDER, 500UL PER KIT

M-DNA-1KB 1/pk
EUR 84
Description: Bioscience Mol Bio; DNA Ladder

FastPure Bacteria DNA Isolation Mini Kit

DC103 100 rxn
EUR 212.4

FastPure Plant DNA Isolation Mini Kit

DC104 50 rxn
EUR 192

FastPure FFPE DNA Isolation Mini Kit

DC105 50 rxn
EUR 241.2

Soil DNA Isolation Mini Kit (50prep)

FASOI-001 50 preps
EUR 189.6

Soil DNA Isolation Mini Kit (100prep)

FASOI-001-1 100 preps
EUR 241.2

Soil DNA Isolation Midi Kit (24prep)

FASOI-002 24 preps
EUR 259.2

Blood and Serum DNA Isolation Kit

K5017100 1 kit
EUR 432

Methylamp Coupled DNA Isolation & Modification Kit

P-1002 40 Samples
EUR 474.12

FitAmp Plasma/Serum DNA Isolation Kit 

P-1004
  • EUR 416.70
  • EUR 149.60
  • EUR 253.00
  • 100 Samples
  • 50 Samples
  • 100 Samples

FitAmp Plasma/Serum DNA Isolation Kit 

P-1004-1 50 Samples
EUR 149.6

FitAmp Plasma/Serum DNA Isolation Kit 

P-1004-2 100 Samples
EUR 253

Genomic DNA Kit (No RNase A)

20-abx098862
  • EUR 477.60
  • EUR 276.00
  • 200 rxns
  • 50 rxns

Rapid Animal Genomic DNA Extraction Kit

AT4780 10Preps
EUR 82.84

Rapid Animal Genomic DNA Extraction Kit

AT4781 50Preps
EUR 151.36

Rapid Animal Genomic DNA Extraction Kit

AT4782 250preps
EUR 425.4

Rapid Blood Genomic DNA Extraction Kit

BT4782 50Preps
EUR 91.97

Rapid Yeast Genomic DNA Extraction Kit

BS8227 50Preps
EUR 91.97

Nucleon BACC3 Genomic DNA Extraction Kit

RPN8512 EACH
EUR 409.26

Plant Genomic DNA extraction buffer

20-abx098075
  • EUR 343.20
  • EUR 126.00
  • 100 ml
  • 1.2 ml

Genomic DNA - Cynomolgus Monkey Male

D1534999-Cy-G01 100 ug
EUR 225.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Cynomolgus Monkey Female

D1534999-Cy-G02 100 ug
EUR 225.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rhesus Monkey Male

D1534999-G01 100 ug
EUR 225.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rhesus Monkey Female

D1534999-G02 100 ug
EUR 225.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Guinea Pig Male

D1G34999-G01 100 ug
EUR 225.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Guinea Pig Female

D1G34999-G02 100 ug
EUR 225.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA Extraction and Purification

EP10012 100 Tests
EUR 606

Genomic DNA Extraction KitAnimal Tissue

FYG102-100P 100 Preps Ask for price

Genomic DNA Extraction KitAnimal Tissue

FYG102-300P 300 Preps Ask for price

Genomic DNA Extraction KitAnimal Tissue

FYG102-50P 50 Preps Ask for price

Genomic DNA Extraction Maxi KitPlant

FYG104-10P 10 Preps Ask for price

Genomic DNA Extraction Maxi KitPlant

FYG104-25P 25 Preps Ask for price

FastPure Cell/Tissue DNA Isolation Mini Kit

DC102 100 rxn
EUR 212.4

FastPure Blood DNA Isolation Mini Kit V2

DC111-01 50 rxn
EUR 214.8

FastPure Blood DNA Isolation Mini Kit V2

DC111-02 200 rxn
EUR 472.8

Soil DNA Plus Isolation Mini Kit (50prep)

FAPSO-001 50 preps
EUR 189.6

Soil DNA Plus Isolation Mini Kit (100prep)

FAPSO-001-1 100 preps
EUR 241.2

FitAmp General Tissue Section DNA Isolation Kit 

P-1003
  • EUR 754.26
  • EUR 253.00
  • EUR 490.60
  • 100 Samples
  • 50 Samples
  • 100 Samples

FitAmp Paraffin Tissue Section DNA Isolation Kit 

P-1009
  • EUR 702.06
  • EUR 239.80
  • EUR 454.30
  • 100 Samples
  • 50 Samples
  • 100 Samples

EpiMag High Throughput DNA Isolation Universal Kit

P-1022-96 96 Samples
EUR 227.7

EpiQuik Circulating Cell-Free DNA Isolation Kit

P-1064
  • EUR 416.70
  • EUR 148.50
  • EUR 253.00
  • 50 Isolations
  • 25 Isolations
  • 50 Isolations

FitAmp General Tissue Section DNA Isolation Kit 

P-1003-1 50 Samples
EUR 253

FitAmp General Tissue Section DNA Isolation Kit 

P-1003-2 100 Samples
EUR 490.6

FitAmp Paraffin Tissue Section DNA Isolation Kit 

P-1009-1 50 Samples
EUR 239.8

FitAmp Paraffin Tissue Section DNA Isolation Kit 

P-1009-2 100 Samples
EUR 454.3

EpiQuik Circulating Cell-Free DNA Isolation Kit

P-1064-25 25 Isolations
EUR 148.5

EpiQuik Circulating Cell-Free DNA Isolation Kit

P-1064-50 50 Isolations
EUR 253

LumiPure genomic DNA from AnySample Kit, 50

21573 50 preps
EUR 129.6

Blood Genomic DNA Kit (0.1-20 ml)

abx098074-For200mlBlood For 200 ml Blood
EUR 427.2

Blood Genomic DNA Kit (0.1-20 ml)

abx098074-For50mlBlood For 50 ml Blood
EUR 260.4

Blood Genomic DNA Kit (5-250 ul)

20-abx098078
  • EUR 493.20
  • EUR 276.00
  • 200 rxns
  • 50 rxns

Bacteria Genomic DNA Kit (No RNase A)

abx098240-50rxns 50 rxns
EUR 276

Food and Fodder Security Genomic DNA Kit

abx098241-50rxns 50 rxns
EUR 376.8

Plant Genomic DNA Kit(No RNase A)

20-abx098863
  • EUR 460.80
  • EUR 276.00
  • 200 rxns
  • 50 rxns

Blood Genomic DNA Kit (No RNase A)

20-abx098864
  • EUR 460.80
  • EUR 276.00
  • 200 rxns
  • 50 rxns

Blood Genomic DNA Kit (with Magnetic Stand)

abx098867-50rxns 50 rxns
EUR 360

One-4-All Genomic DNA Miniprep Kit

BS88503 50Preps
EUR 140.39

One-4-All Genomic DNA Miniprep Kit

BS88504 100preps
EUR 204.7

One-4-All Genomic DNA Miniprep Kit

BS88505 250preps
EUR 393.55

Mouse Tail Genomic DNA Extraction Kit(250)

M9100-250 250 prep
EUR 643.2

Blood genomic DNA extraction and purification kit

K1443-100
EUR 574.8

Blood Genomic DNA Extraction Mini Kit (100prep)

FABGK-100 100 preps
EUR 198

Blood Genomic DNA Extraction Mini Kit (300prep)

FABGK-300 300 preps
EUR 319.2

FavorPrep Genomic DNA Clean-Up Kit (50prep)

FAGDC-001 50 preps
EUR 154.8

FavorPrep Genomic DNA Clean-Up Kit (200prep)

FAGDC-001-1 200 preps
EUR 244.8

Plant Genomic DNA Extraction Mini Kit (50prep)

FAPGK-001 50 preps
EUR 189.6

Plant Genomic DNA Extraction Mini Kit (100prep)

FAPGK-001-1 100 preps
EUR 244.8

Plant Genomic DNA Extraction Mini Kit (200prep)

FAPGK-001-2 200 preps
EUR 328.8

Plant Genomic DNA Extraction Maxi Kit (10prep)

FAPGK-002 10 preps
EUR 241.2

Plant Genomic DNA Extraction Maxi Kit (24prep)

FAPGK-002-1 24 preps
EUR 380.4

One-tube Plant Genomic DNA Extraction Kit

PT71816 100preps
EUR 105.67

To further explore the molecular mechanisms underlying the SCE therapy and determine the actions of CB-SC-release exosomes, we investigate phagocytizizes these exosomes during treatment with CB-SC. By co-cultivating DIO CB-SC-SC-labeled exosomes with human peripheral blood mononuclear cells (PBMC), we have found that CB-SC-derivative exosomes were mainly taken up by CD14-positive human monocytes, resulting in the Differentiation of monocytes in type 2 macrophages (M2), with morphology and expression resembling the spindle of molecular markers associated with M2.