Development of a CD63 Aptamer for Efficient Cancer Immunochemistry and Immunoaffinity-Based Exosome Isolation

CD63, members of Transmembrane-4-Superfamili Tetraspanin proteins and very n-glycosposomal membrane membrane proteins are known to regulate the malignancy of various types of cancer such as melanoma and breast cancer and function as potential markers for cancer detection. Recently, an important role as a classic exosome marker was also emphasized. In this work, through using a competitive Selex based magnetic beads (systematic evolution of ligands with exponential enrichment) and introducing NaCl 0.5m as an elution buffer, we identify two DNA aptamers (CD63-1 and CD63-2) with high affinity. and specificity for CD63 protein (KD = 38.71nm and 78.43, respectively).

Furthermore, CD63-1 was found efficiently in binding CD63 positive cells, including MDA-MB-231 cell breast cancer and too much HEK293T cells are expressed, with intermediate fastener affinity (KD ~ 100 Nm) as assessed by Flow cytometry. When the immunostaining test was carried out using clinical breast cancer biopsy, the CD63-1 APTIMER showed a comparable diagnostic efficacy for CD63 positive breast cancer with commercial antibodies. After developing the immunoaffinity imunoaffinity beads-based immunoaffinity system using Aptamer CD63-1, it was found that this bead-based system could effectively isolate the exosomes of MDA-MB-231 cell culture media and HT29.

Importantly, the introduction of Elution NaCl in this work allows the original exosom isolation through a simple 0.5m NaCl incubation step. Based on this result, we strongly believe that the aptamers developed can be useful for the exosom insulation of the original state efficiently from clinical samples and various terorostic applications for positive CD63 cancer.

Deposition of peptide affinity from extracellular vesicles and cell-free DNA improve sequencing performance for pathogenic mutation detection in plasma lung cancer patients

Liquid biopsy is a minimal-invasive diagnostic method that can increase access to molecular profiles for non-small patients lung cancer (NSCLC). Although the insulation of DNA (CF-DNA) cells from plasma is a standard liquid biopsy method to detect DNA mutations in cancer patients, its sensitivity can vary greatly. VN96 is a peptide with affinity for extracellular vesicles (EV) and circulating CF-DNA. In this study, we evaluated whether the Peptide-Affinity (PA) of EVS and CF-DNA precipitation of Plasma NSCLC patients increased the sensitivity of a single nucleotide variant (SNV) and compared with the observed SNV with those reported in suitable network biopsy.

Pope NSCLC patients are the target of PA precipitation or cell-free methods and total nucleic acids (TNA) are extracted; SNV is then detected by the next generation (NGS) sequencing. Pa causes an increase in DNA recovery and an increase in sequencing parameters of NGS when compared to CF-TNA. Reduced concordance with the network was observed in PA-TNA (62%) compared to CF-TNA (81%), mainly because of the identification of SNVs in Pa-TNA which were not observed in the network. The EGFR mutation was detected in PA-TNA with 83% sensitivity and 100% specificity.

In conclusion, PA-TNA can increase the abundant low allel detection limit using NGS. Stem Cell Educator Therapy (SCE) is a new clinical approach to the treatment of type 1 diabetes and other autoimmune diseases. SCE therapy circulates mononuclear blood cells isolated patients (for example, lymphocytes and monocytes) through apheresis machines, ko-culture mononuclear blood patients with stem cells that are reduced by the blood of adhered straps (CB-SC) on the SCE device, and then return ” Educated “these immune cells with patient blood. Exosom is a nano-sized extracellular vesicle between 30-150 nm in all biofluid media and cell culture.

 Development of a CD63 Aptamer for Efficient Cancer Immunochemistry and Immunoaffinity-Based Exosome Isolation
Development of a CD63 Aptamer for Efficient Cancer Immunochemistry and Immunoaffinity-Based Exosome Isolation

Clinical applications of extracellular vesicles of mesenchymal stem / stromal cells: therapeutic potential of an acellular product

Over the past decade, the sequery activity of mesenchymal stem cells (MSCS) has been widely studied due to its possible therapeutic role. In fact, MSCS releases extracellular vesicles (EVS) containing relevant biomolecules such as MRNAS, microarnas, bioactive lipids and signaling receptors, capable of restoring physiological conditions in which regenerative or anti-inflammatory actions are required. A real benefit would come from the therapeutic use of EV relative to MSC, avoiding possible immune release, pulmonary trapping, safety improvement and allowing crossing of organic barriers. A number of concerns must still be resolved concerning the mechanisms determining the beneficial effect of the MSC-EVS, the possible modification of their properties as a result of isolation / purification methods and / or the best approach for large production. scale for clinical use.

Most of the preclinical studies have been successful, reporting the role of MSC-EVS a role of protection in acute kidney injury after the opposing ischemia, powerful anti-inflammatory and anti-fibrotic effects by reducing inflammation Associated with diseases and fibrosis in lung and liver, as well as the modulation of innate and adaptive immune responses in transplant disease compared to host diseases (GVHD) as well as autoimmune diseases. However, the translation of MSC-EVS to the clinical stage is still in the initial phase. In this document, we discuss the therapeutic potential of an acellular product such as MSC derivative EVS (MSC-EVS) in acute and chronic pathologies.

Bacterial Genomic DNA Isolation Kit

K309-100
EUR 376

Easy3-Plant Genomic DNA Isolation Kit

FYG503-100P 100 Preps Ask for price

Easy3-Plant Genomic DNA Isolation Kit

FYG503-500P 500 Preps Ask for price

Mammalian Cell Genomic DNA Isolation Kit

K967-100
EUR 381

Plant Tissue Genomic DNA Isolation Kit

K316-100
EUR 376

Rapid Bacteria Genomic DNA Isolation Kit

BS8225 50Preps
EUR 76.64
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Bacteria

Rapid Fungal Genomic DNA Isolation Kit

FT71415 50Preps
EUR 76.64
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Fungi

One-Tube Bacteria Genomic DNA Isolation Kit

BS8413 100preps
EUR 78.93
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Bacteria

One-Tube Bacteria Genomic DNA Isolation Kit

BS8414 500Preps, 500prep
EUR 165.71
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Bacteria

Genomic DNA Kit

20-abx098076
  • EUR 439.00
  • EUR 244.00
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

Mitochondrial DNA Isolation Kit

55R-1361 50 assays
EUR 825
Description: Mitochondrial DNA Isolation Kit for use in the research laboratory

Saliva DNA Isolation Kit

K5011050 1 kit
EUR 256

Urine DNA Isolation Kit

K5011150 1 kit
EUR 256

Stool DNA Isolation Kit

K5011250 1 kit
EUR 256

Serum DNA Isolation Kit

K5018100 1 kit
EUR 360

Mitochondrial DNA Isolation Kit

K280-50
EUR 588

Mitochondrial DNA Isolation Kit

K2117-50 50 assays
EUR 599

Plant Genomic DNA Kit

20-abx098077
  • EUR 411.00
  • EUR 230.00
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

Bacteria Genomic DNA Kit

20-abx098080
  • EUR 105.00
  • EUR 244.00
  • 2 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

Micro Genomic DNA Kit

20-abx098242
  • EUR 105.00
  • EUR 328.00
  • 2 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

Blood Genomic DNA Kit

abx098868-50rxns 50 rxns
EUR 272
  • Shipped within 5-10 working days.

Plant Genomic DNA Kit

abx294004-50preps 50 preps
EUR 398
  • Shipped within 5-12 working days.

Animal Genomic DNA Kit

abx294005-100preps 100 preps
EUR 523
  • Shipped within 5-12 working days.

Animal Genomic DNA Kit

abx294005-50preps 50 preps
EUR 398
  • Shipped within 5-12 working days.

Genomic DNA Extraction Kit

K5016005 1 kit
EUR 329

Soil Genomic DNA Kit

K1411-250
EUR 561

Soil Genomic DNA Kit

K1411-50
EUR 316

Insect Genomic DNA Kit

K1412-250
EUR 561

Insect Genomic DNA Kit

K1412-50
EUR 316

Mollusc Genomic DNA Kit

K1413-250
EUR 561

Mollusc Genomic DNA Kit

K1413-50
EUR 316

Yeast Genomic DNA Kit

K1414-250
EUR 561

Yeast Genomic DNA Kit

K1414-50
EUR 316

Fungal Genomic DNA Kit

K1415-250
EUR 561

Fungal Genomic DNA Kit

K1415-50
EUR 316

Human Genomic DNA

BIO-35025 500µl @ 200ng/µl Ask for price

Human Genomic DNA

X11000 0.2 ml Ask for price
Description: reagents widely cited

Whole Blood DNA Isolation Kit

K528-100
EUR 376

Apoptotic DNA Ladder Isolation Kit

K2045-50 50 assays
EUR 502

Apoptotic DNA Ladder Isolation Kit

K170-50
EUR 468

FitAmp Gel DNA Isolation Kit

P-1007 100 Samples
EUR 270.4
Description: The best epigenetics products

FitAmp Urine DNA Isolation Kit

P-1017 100 Samples
EUR 395.1
Description: kits suitable for this type of research

XCF Exosomal DNA Isolation Kit

XCF200A-1 20 rxn
EUR 386
  • Category: Exosome

Marine Animal Genomic DNA Kit

20-abx098079
  • EUR 105.00
  • EUR 244.00
  • 2 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

FFPE Tissue Genomic DNA Kit

abx098866-50rxns 50 rxns
EUR 272
  • Shipped within 5-10 working days.

ISOLATE II Genomic DNA Kit

BIO-52065 10 preps Ask for price

ISOLATE II Genomic DNA Kit

BIO-52066 50 preps Ask for price

ISOLATE II Genomic DNA Kit

BIO-52067 250 preps Ask for price

AccuRT Genomic DNA Removal Kit

G488 200 Reactions
EUR 82

Zenoquick Genomic DNA Purification Kit

Z2003-200 200 preps
EUR 631

Zenoprep Genomic DNA Purification Kit

Z2004-400 400 preps
EUR 550

Genomic DNA - Mouse Male

D1334999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Mouse Female

D1334999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rat Male

D1434999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rat Female

D1434999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Dog Male

D1734999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Dog Female

D1734999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rabbit Male

D1834999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rabbit Female

D1834999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Porcine Male

D1934999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Porcine Female

D1934999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Bovine Male

D1B34999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Bovine Female

D1B34999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Chicken Male

D1C34999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Chicken Female

D1C34999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Hamster Male

D1H34999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Hamster Female

D1H34999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Horse Male

D1O34999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Horse Female

D1O34999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Human Male

D1234999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Human Female

D1234999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Human Brain Genomic DNA

X11001 10 µg Ask for price
Description: kits suitable for this type of research

FastPure Bacteria DNA Isolation Mini Kit

DC103 100 rxn
EUR 177

FastPure Plant DNA Isolation Mini Kit

DC104 50 rxn
EUR 160

FastPure FFPE DNA Isolation Mini Kit

DC105 50 rxn
EUR 201

Soil DNA Isolation Mini Kit (50prep)

FASOI-001 50 preps
EUR 158

Soil DNA Isolation Mini Kit (100prep)

FASOI-001-1 100 preps
EUR 201

Soil DNA Isolation Midi Kit (24prep)

FASOI-002 24 preps
EUR 216

Blood and Serum DNA Isolation Kit

K5017100 1 kit
EUR 360

Methylamp Coupled DNA Isolation & Modification Kit

P-1002 40 Samples
EUR 395.1
Description: The best epigenetics products

FitAmp Plasma/Serum DNA Isolation Kit

P-1004 100 Samples
EUR 347.25
Description: fast delivery possible

100 BP DNA LADDER, 500UL PER KIT

M-DNA-100BP 1/pk
EUR 73
Description: Bioscience Mol Bio; DNA Ladder

1 KB DNA LADDER, 500UL PER KIT

M-DNA-1KB 1/pk
EUR 70
Description: Bioscience Mol Bio; DNA Ladder

Genomic DNA Kit (No RNase A)

20-abx098862
  • EUR 398.00
  • EUR 230.00
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

Rapid Animal Genomic DNA Extraction Kit

AT4780 10Preps
EUR 69.03
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Animal

Rapid Animal Genomic DNA Extraction Kit

AT4781 50Preps
EUR 126.13
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Animal

Rapid Animal Genomic DNA Extraction Kit

AT4782 250preps
EUR 354.5
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Animal

Rapid Yeast Genomic DNA Extraction Kit

BS8227 50Preps
EUR 76.64
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Yeast

Rapid Blood Genomic DNA Extraction Kit

BT4782 50Preps
EUR 76.64
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Blood

Genomic DNA - Cynomolgus Monkey Male

D1534999-Cy-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Cynomolgus Monkey Female

D1534999-Cy-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rhesus Monkey Male

D1534999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rhesus Monkey Female

D1534999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Guinea Pig Male

D1G34999-G01 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Guinea Pig Female

D1G34999-G02 100 ug
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Plant Genomic DNA extraction buffer

20-abx098075
  • EUR 286.00
  • EUR 105.00
  • 100 ml
  • 1.2 ml
  • Shipped within 5-10 working days.

Genomic DNA Extraction KitAnimal Tissue

FYG102-100P 100 Preps Ask for price

Genomic DNA Extraction KitAnimal Tissue

FYG102-300P 300 Preps Ask for price

Genomic DNA Extraction KitAnimal Tissue

FYG102-50P 50 Preps Ask for price

Genomic DNA Extraction Maxi KitPlant

FYG104-10P 10 Preps Ask for price

Genomic DNA Extraction Maxi KitPlant

FYG104-25P 25 Preps Ask for price

Genomic DNA Extraction and Purification

EP10012 100 Tests
EUR 505

FastPure Cell/Tissue DNA Isolation Mini Kit

DC102 100 rxn
EUR 177

FastPure Blood DNA Isolation Mini Kit V2

DC111-01 50 rxn
EUR 179

FastPure Blood DNA Isolation Mini Kit V2

DC111-02 200 rxn
EUR 394

Soil DNA Plus Isolation Mini Kit (50prep)

FAPSO-001 50 preps
EUR 158

Soil DNA Plus Isolation Mini Kit (100prep)

FAPSO-001-1 100 preps
EUR 201

FitAmp General Tissue Section DNA Isolation Kit

P-1003 100 Samples
EUR 628.55
Description: kits suitable for this type of research

FitAmp Paraffin Tissue Section DNA Isolation Kit

P-1009 100 Samples
EUR 585.05
Description: Ask the seller for details

EpiQuik Circulating Cell-Free DNA Isolation Kit

P-1064 50 Isolations
EUR 347.25
Description: kits suitable for this type of research

LumiPure genomic DNA from AnySample Kit, 50

21573 50 preps
EUR 108

Blood Genomic DNA Kit (0.1-20 ml)

abx098074-For200mlBlood For 200 ml Blood
EUR 356
  • Shipped within 5-10 working days.

Blood Genomic DNA Kit (0.1-20 ml)

abx098074-For50mlBlood For 50 ml Blood
EUR 217
  • Shipped within 5-10 working days.

Blood Genomic DNA Kit (5-250 ul)

20-abx098078
  • EUR 411.00
  • EUR 230.00
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

Bacteria Genomic DNA Kit (No RNase A)

abx098240-50rxns 50 rxns
EUR 230
  • Shipped within 5-10 working days.

Food and Fodder Security Genomic DNA Kit

abx098241-50rxns 50 rxns
EUR 314
  • Shipped within 5-10 working days.

Plant Genomic DNA Kit(No RNase A)

20-abx098863
  • EUR 384.00
  • EUR 230.00
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

Blood Genomic DNA Kit (No RNase A)

20-abx098864
  • EUR 384.00
  • EUR 230.00
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

Blood Genomic DNA Kit (with Magnetic Stand)

abx098867-50rxns 50 rxns
EUR 300
  • Shipped within 5-10 working days.

Blood Genomic DNA Extraction Mini Kit (100prep)

FABGK-100 100 preps
EUR 165

Blood Genomic DNA Extraction Mini Kit (300prep)

FABGK-300 300 preps
EUR 266

FavorPrep Genomic DNA Clean-Up Kit (50prep)

FAGDC-001 50 preps
EUR 129

FavorPrep Genomic DNA Clean-Up Kit (200prep)

FAGDC-001-1 200 preps
EUR 204

Plant Genomic DNA Extraction Mini Kit (50prep)

FAPGK-001 50 preps
EUR 158

Plant Genomic DNA Extraction Mini Kit (100prep)

FAPGK-001-1 100 preps
EUR 204

Plant Genomic DNA Extraction Mini Kit (200prep)

FAPGK-001-2 200 preps
EUR 274

Plant Genomic DNA Extraction Maxi Kit (10prep)

FAPGK-002 10 preps
EUR 201

Plant Genomic DNA Extraction Maxi Kit (24prep)

FAPGK-002-1 24 preps
EUR 317

Blood genomic DNA extraction and purification kit

K1443-100
EUR 479

Mouse Tail Genomic DNA Extraction Kit(250)

M9100-250 250 prep
EUR 536

T-Pro Genomic DNA Midi Kit (20)

RB94-NGM020 20preps/kit
EUR 222

T-Pro Genomic DNA Mini Kit (100)

RB94-NGS100 100preps/kit
EUR 213

One-4-All Genomic DNA Miniprep Kit

BS88503 50Preps
EUR 116.99
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Multipurpose

One-4-All Genomic DNA Miniprep Kit

BS88504 100preps
EUR 170.58
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Multipurpose

One-4-All Genomic DNA Miniprep Kit

BS88505 250preps
EUR 327.96
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Multipurpose

One-tube Plant Genomic DNA Extraction Kit

PT71816 100preps
EUR 88.06
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Plant

One-tube Plant Genomic DNA Extraction Kit

PT71817 500Preps, 500prep
EUR 202.25
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Plant

One Tube Viral DNA Isolation Buffer

VT4785 50Preps
EUR 187.03
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Viral

One Tube Viral DNA Isolation Buffer

VT4786 100preps
EUR 324.05
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Viral

Hipro Circulating Cell-Free DNA (cfDNA) Isolation Kit

W2603-10 NULL
EUR 0

Hipro Circulating Cell-Free DNA (cfDNA) Isolation Kit

W2603-20 NULL
EUR 0

Hipro Circulating Cell-Free DNA (cfDNA) Isolation Kit

W2603-50 NULL
EUR 0

Genomic DNA Extraction Mini KitPlant Tissue

FYG103-100P 100 Preps Ask for price

Fully CpG Methylated Human Genomic DNA

X10000 10 µg/100 µl Ask for price
Description: The best epigenetics products

LumiPure genomic DNA Blood and Buccal Kit, 50

21553 50 preps
EUR 97

Marine Animal Genomic DNA Kit (No RNase A)

abx298012-50rxns 50 rxns
EUR 230
  • Shipped within 5-10 working days.

To further explore the molecular mechanisms underlying the SCE therapy and determine the actions of CB-SC-release exosomes, we investigate phagocytizizes these exosomes during treatment with CB-SC. By co-cultivating DIO CB-SC-SC-labeled exosomes with human peripheral blood mononuclear cells (PBMC), we have found that CB-SC-derivative exosomes were mainly taken up by CD14-positive human monocytes, resulting in the Differentiation of monocytes in type 2 macrophages (M2), with morphology and expression resembling the spindle of molecular markers associated with M2.