Development of a CD63 Aptamer for Efficient Cancer Immunochemistry and Immunoaffinity-Based Exosome Isolation

CD63, members of Transmembrane-4-Superfamili Tetraspanin proteins and very n-glycosposomal membrane membrane proteins are known to regulate the malignancy of various types of cancer such as melanoma and breast cancer and function as potential markers for cancer detection. Recently, an important role as a classic exosome marker was also emphasized. In this work, through using a competitive Selex based magnetic beads (systematic evolution of ligands with exponential enrichment) and introducing NaCl 0.5m as an elution buffer, we identify two DNA aptamers (CD63-1 and CD63-2) with high affinity. and specificity for CD63 protein (KD = 38.71nm and 78.43, respectively).

Furthermore, CD63-1 was found efficiently in binding CD63 positive cells, including MDA-MB-231 cell breast cancer and too much HEK293T cells are expressed, with intermediate fastener affinity (KD ~ 100 Nm) as assessed by Flow cytometry. When the immunostaining test was carried out using clinical breast cancer biopsy, the CD63-1 APTIMER showed a comparable diagnostic efficacy for CD63 positive breast cancer with commercial antibodies. After developing the immunoaffinity imunoaffinity beads-based immunoaffinity system using Aptamer CD63-1, it was found that this bead-based system could effectively isolate the exosomes of MDA-MB-231 cell culture media and HT29.

Importantly, the introduction of Elution NaCl in this work allows the original exosom isolation through a simple 0.5m NaCl incubation step. Based on this result, we strongly believe that the aptamers developed can be useful for the exosom insulation of the original state efficiently from clinical samples and various terorostic applications for positive CD63 cancer.

Deposition of peptide affinity from extracellular vesicles and cell-free DNA improve sequencing performance for pathogenic mutation detection in plasma lung cancer patients

Liquid biopsy is a minimal-invasive diagnostic method that can increase access to molecular profiles for non-small patients lung cancer (NSCLC). Although the insulation of DNA (CF-DNA) cells from plasma is a standard liquid biopsy method to detect DNA mutations in cancer patients, its sensitivity can vary greatly. VN96 is a peptide with affinity for extracellular vesicles (EV) and circulating CF-DNA. In this study, we evaluated whether the Peptide-Affinity (PA) of EVS and CF-DNA precipitation of Plasma NSCLC patients increased the sensitivity of a single nucleotide variant (SNV) and compared with the observed SNV with those reported in suitable network biopsy.

Pope NSCLC patients are the target of PA precipitation or cell-free methods and total nucleic acids (TNA) are extracted; SNV is then detected by the next generation (NGS) sequencing. Pa causes an increase in DNA recovery and an increase in sequencing parameters of NGS when compared to CF-TNA. Reduced concordance with the network was observed in PA-TNA (62%) compared to CF-TNA (81%), mainly because of the identification of SNVs in Pa-TNA which were not observed in the network. The EGFR mutation was detected in PA-TNA with 83% sensitivity and 100% specificity.

In conclusion, PA-TNA can increase the abundant low allel detection limit using NGS. Stem Cell Educator Therapy (SCE) is a new clinical approach to the treatment of type 1 diabetes and other autoimmune diseases. SCE therapy circulates mononuclear blood cells isolated patients (for example, lymphocytes and monocytes) through apheresis machines, ko-culture mononuclear blood patients with stem cells that are reduced by the blood of adhered straps (CB-SC) on the SCE device, and then return ” Educated “these immune cells with patient blood. Exosom is a nano-sized extracellular vesicle between 30-150 nm in all biofluid media and cell culture.

 Development of a CD63 Aptamer for Efficient Cancer Immunochemistry and Immunoaffinity-Based Exosome Isolation
Development of a CD63 Aptamer for Efficient Cancer Immunochemistry and Immunoaffinity-Based Exosome Isolation

Clinical applications of extracellular vesicles of mesenchymal stem / stromal cells: therapeutic potential of an acellular product

Over the past decade, the sequery activity of mesenchymal stem cells (MSCS) has been widely studied due to its possible therapeutic role. In fact, MSCS releases extracellular vesicles (EVS) containing relevant biomolecules such as MRNAS, microarnas, bioactive lipids and signaling receptors, capable of restoring physiological conditions in which regenerative or anti-inflammatory actions are required. A real benefit would come from the therapeutic use of EV relative to MSC, avoiding possible immune release, pulmonary trapping, safety improvement and allowing crossing of organic barriers. A number of concerns must still be resolved concerning the mechanisms determining the beneficial effect of the MSC-EVS, the possible modification of their properties as a result of isolation / purification methods and / or the best approach for large production. scale for clinical use.

Most of the preclinical studies have been successful, reporting the role of MSC-EVS a role of protection in acute kidney injury after the opposing ischemia, powerful anti-inflammatory and anti-fibrotic effects by reducing inflammation Associated with diseases and fibrosis in lung and liver, as well as the modulation of innate and adaptive immune responses in transplant disease compared to host diseases (GVHD) as well as autoimmune diseases. However, the translation of MSC-EVS to the clinical stage is still in the initial phase. In this document, we discuss the therapeutic potential of an acellular product such as MSC derivative EVS (MSC-EVS) in acute and chronic pathologies.

Easy3-Plant Genomic DNA Isolation Kit

FYG503-100P 100 Preps Ask for price

Easy3-Plant Genomic DNA Isolation Kit

FYG503-500P 500 Preps Ask for price

Rapid Bacteria Genomic DNA Isolation Kit

BS8225 50Preps
EUR 91.97

Mammalian Cell Genomic DNA Isolation Kit

K967-100 each
EUR 457.2

One-Tube Bacteria Genomic DNA Isolation Kit

BS8413 100preps
EUR 94.72

One-Tube Bacteria Genomic DNA Isolation Kit

BS8414 500Preps, 500prep
EUR 198.85

Genoirse Plant DNA Isolation Kit

GR101083 50
EUR 223

ISOLATE II Genomic DNA Kit

BIO-52065 10 preps Ask for price

ISOLATE II Genomic DNA Kit

BIO-52066 50 preps Ask for price

ISOLATE II Genomic DNA Kit

BIO-52067 250 preps Ask for price

Urine DNA Isolation Kit

K5011150 1 kit
EUR 237

Stool DNA Isolation Kit

K5011250 1 kit
EUR 237

Serum DNA Isolation Kit

K5018100 1 kit
EUR 355

Saliva DNA Isolation Kit

K5011050 1 kit
EUR 237

Mitochondrial DNA Isolation Kit

55R-1361 50 assays
EUR 990
Description: Mitochondrial DNA Isolation Kit for use in the research laboratory

Mitochondrial DNA Isolation Kit

K280-50 each
EUR 705.6

Mitochondrial DNA Isolation Kit

K2117-50 50 assays
EUR 718.8

FitAmp Gel DNA Isolation Kit

P-1007 100 Samples
EUR 324.48

Whole Blood DNA Isolation Kit

K528-100 each
EUR 451.2

FitAmp Urine DNA Isolation Kit 

P-1017
  • EUR 474.12
  • EUR 171.60
  • EUR 292.60
  • 100 Samples
  • 50 Samples
  • 100 Samples

XCF Exosomal DNA Isolation Kit

XCF200A-1 20 rxn
EUR 328

FastPure FFPE DNA Isolation Kit

DC105-01 50 rxns
EUR 75.2

Blood and Serum DNA Isolation Kit

K5017100 1 kit
EUR 355

Apoptotic DNA Ladder Isolation Kit

K170-50 each
EUR 561.6

Apoptotic DNA Ladder Isolation Kit

K2045-50 50 assays
EUR 602.4

FitAmp General Tissue Section DNA Isolation Kit 

P-1003
  • EUR 754.26
  • EUR 253.00
  • EUR 490.60
  • 100 Samples
  • 50 Samples
  • 100 Samples

FitAmp Plasma/Serum DNA Isolation Kit 

P-1004
  • EUR 416.70
  • EUR 149.60
  • EUR 253.00
  • 100 Samples
  • 50 Samples
  • 100 Samples

Soil DNA Isolation Mini Kit (100prep)

FASOI-001-1 100 preps
EUR 241.2

Soil DNA Isolation Mini Kit (50prep)

FASOI-001 50 preps
EUR 189.6

Soil DNA Isolation Midi Kit (24prep)

FASOI-002 24 preps
EUR 259.2

FastPure FFPE DNA Isolation Mini Kit

DC105 50 rxn
EUR 241.2

FastPure Plant DNA Isolation Mini Kit

DC104 50 rxn
EUR 192

FastPure Plant DNA Isolation Mini Kit

DC104-01 50 rxns
EUR 60

FastPure Bacteria DNA Isolation Mini Kit

DC103 100 rxn
EUR 212.4

FastPure Bacteria DNA Isolation Mini Kit

DC103-01 100 rxns
EUR 52

FitAmp Paraffin Tissue Section DNA Isolation Kit 

P-1009
  • EUR 702.06
  • EUR 239.80
  • EUR 454.30
  • 100 Samples
  • 50 Samples
  • 100 Samples

FastPure Blood DNA Isolation Mini Kit V2

DC111-01 50 rxn
EUR 214.8

FastPure Blood DNA Isolation Mini Kit V2

DC111-02 200 rxn
EUR 472.8

EpiQuik Circulating Cell-Free DNA Isolation Kit

P-1064
  • EUR 416.70
  • EUR 148.50
  • EUR 253.00
  • 50 Isolations
  • 25 Isolations
  • 50 Isolations

Soil DNA Plus Isolation Mini Kit (100prep)

FAPSO-001-1 100 preps
EUR 241.2

FastPure Cell/Tissue DNA Isolation Mini Kit

DC102 100 rxn
EUR 212.4

FastPure Cell/Tissue DNA Isolation Mini Kit

DC102-01 100 rxns
EUR 52

Soil DNA Plus Isolation Mini Kit (50prep)

FAPSO-001 50 preps
EUR 189.6

Methylamp Coupled DNA Isolation & Modification Kit

P-1002 40 Samples
EUR 474.12

One Tube Viral DNA Isolation Buffer

VT4785 50Preps
EUR 224.44

One Tube Viral DNA Isolation Buffer

VT4786 100preps
EUR 388.86

EpiMag High Throughput DNA Isolation Universal Kit

P-1022-96 96 Samples
EUR 227.7

Hipro Circulating Cell-Free DNA (cfDNA) Isolation Kit

W2603-10 - Ask for price

Hipro Circulating Cell-Free DNA (cfDNA) Isolation Kit

W2603-20 - Ask for price

Hipro Circulating Cell-Free DNA (cfDNA) Isolation Kit

W2603-50 - Ask for price

Rna Isolation Kit

11828665001 EACH
EUR 378.62

miRNA Isolation Kit

FAMIK--001 Rxns: 100
EUR 398.4
Description: Contents of the kit:
  • Rxns:100Lysis
  • Buffer-10 ml
  • Acid-Phenol-10 ml
  • RNA Column and Collection Tube-100 sets
  • Wash Buffer-10 ml
  • Release Buffer-0.5 ml

miRNA Isolation Kit

FAMIK--002 Rxns: 50
EUR 267.6

miRNA Isolation Kit

FYG304-100P 100 Preps Ask for price

MicroRNA Isolation Kit

KS341025 1 kit
EUR 247

Endosome Isolation Kit

P528 - Ask for price

Microsome Isolation Kit

K249-50 each
EUR 633.6

FastPure Blood/Cell/Tissue/Bacteria DNA Isolation Mini Kit

DC112-01 50 rxns
EUR 63.6

FastPure Blood/Cell/Tissue/Bacteria DNA Isolation Mini Kit

DC112-02 200 rxns
EUR 223.2

Mitochondria Isolation Kit

AR0156 1 kit
EUR 127.2

Preadipocyte Isolation Kit

K583-5 each
EUR 639.6

Total RNA Isolation Kit

P-9100 50 samples
EUR 416.7

Easy3-RNA Isolation Kit

FYG501-100P 100 Preps Ask for price

Easy3-RNA Isolation Kit

FYG501-500P 500 Preps Ask for price

Stool DNA Isolation Mini Kit (100prep), with Proteinase K Powder

FASTI001-1 100 preps
EUR 261.6

Yeast Nuclei Isolation Kit

K289-50 each
EUR 470.4

Stool DNA Isolation Mini Kit (50prep), with Proteinase K Powder

FASTI001 50 preps
EUR 198

Lipid Droplet Isolation Kit

MET-5011 50 preps
EUR 498
Description: Lipid droplets are lipid rich organelles found in the adipose tissue of eukaryotes. They function to regulate the hydrolysis and storage of neutral lipids and serve as storage for cholesterol and acyl-glycerols. Lipid droplets have also been associated with inflammatory responses, obesity, atherosclerosis, and cancer.

ExoPure? Isolation Kit   (Urine)

K1240-10 each
EUR 836.4

ExoPure? Isolation Kit   (Urine)

K1240-2 each
EUR 483.6

Cartilage RNA Isolation Kit

K2031010 1 kit
EUR 443

Apoptotic Cell Isolation Kit

55R-1349 30 units
EUR 874.8
Description: Apoptotic Cell Isolation Kit for use in the research laboratory

Apoptotic Cell Isolation Kit

K258-30 each
EUR 561.6

Total RNA Isolation Kit II

FATRS--001 100 preps
EUR 369.6

Total RNA Isolation Kit II

FATRS--050 50 preps
EUR 259.2

EVery EV RNA Isolation Kit

EVery100B-1 20 preps
EUR 412

EpiQuik Circulating Cell-Free DNA (ccfDNA) Isolation Easy Kit

P-1065
  • EUR 416.70
  • EUR 148.50
  • EUR 253.00
  • 50 Isolations
  • 25 Isolations
  • 50 Isolations

Exo-Urine EV Isolation Kit

EXOU100A-1 2 x 5 reactions
EUR 490

Cell Mitochondria Isolation Kit

abx090630-50100tests 50-100 tests
EUR 309.6

Rapid Blood RNA Isolation Kit

BT4182 10Preps
EUR 98.36

Rapid Blood RNA Isolation Kit

BT4183 50Preps
EUR 197.03

Rapid Blood RNA Isolation Kit

BT4184 250preps
EUR 608.1

Total RNA Isolation Kit (Plant)

P-9104 50 samples
EUR 416.7

Rapid Plant RNA Isolation Kit

PT4191 50Preps
EUR 95.63

Yeast Mitochondria Isolation Kit

K259-50 each
EUR 502.8

Total RNA Isolation Kit (Tissue)

P-9102 50 samples
EUR 416.7

EpiMag Viral RNA Isolation Kit

P-9108-050 50 samples
EUR 152.9

Tissue Mitochondria Isolation Kit

abx090631-50100assays 50-100 assays
EUR 309.6

Genomic DNA Kit

20-abx098076
  • EUR 526.80
  • EUR 292.80
  • 200 rxns
  • 50 rxns

Lysosome Isolation Kit (20 tests)

P534 - Ask for price

Exosome Isolation kit (for urine)

P120L - Ask for price

Exosome Isolation kit (for urine)

P120S - Ask for price

Easy3-Plant RNA Isolation Kit

FYG502-100P 100 Preps Ask for price

Easy3-Plant RNA Isolation Kit

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CD19 Positive Cell Isolation Kit

78564-1 1 x 10^8 Cells
EUR 70
Description: The CD19 Positive Cell Isolation Kit is designed to magnetically separate CD19-expressing-cells from a complex immune cell population. This kit is optimized for the isolation of CD19 positive cells from normal human peripheral blood mononuclear cells (PBMCs). Cells are incubated with the antibody:bead complex and placed on a magnet for quick and easy separation. When placed on the magnet, CD19-positive cells will be immobilized along the side of the tube while undesired CD19-negative cells will remain in suspension for easy removal.

CD19 Positive Cell Isolation Kit

78564-2 1 x 10^9 Cells
EUR 630
Description: The CD19 Positive Cell Isolation Kit is designed to magnetically separate CD19-expressing-cells from a complex immune cell population. This kit is optimized for the isolation of CD19 positive cells from normal human peripheral blood mononuclear cells (PBMCs). Cells are incubated with the antibody:bead complex and placed on a magnet for quick and easy separation. When placed on the magnet, CD19-positive cells will be immobilized along the side of the tube while undesired CD19-negative cells will remain in suspension for easy removal.

Rapid Bacteria RNA Isolation Kit

BS8625 50Preps
EUR 95.63

ExoPure? Isolation Kit   (Cell Media)

K1237-10 each
EUR 796.8

ExoPure? Isolation Kit   (Cell Media)

K1237-2 each
EUR 470.4

ExoPure? Isolation Kit   (Bio Fluids)

K1241-10 each
EUR 836.4

ExoPure? Isolation Kit   (Bio Fluids)

K1241-2 each
EUR 483.6

Lipopolysaccharide (LPS) Isolation Kit

K438-10 each
EUR 333.6

ExoPure? Isolation Kit   (Serum, Plasma)

K1238-10 each
EUR 836.4

ExoPure? Isolation Kit   (Serum, Plasma)

K1238-2 each
EUR 470.4

Cell Surface Protein Isolation Kit

K295-10 each
EUR 601.2

EZExtract? Core Histone Isolation Kit

K234-100 each
EUR 314.4

10-min. Single Cell Isolation Kit

P713-20 - Ask for price

MagIso NK Cell Isolation Kit, Human

WHK-B023 20 tests, 200 tests Ask for price

Circulating Nuleic Acid Isolation Kit

FACFK-050 50 preps
EUR 589.2

Circulating Nuleic Acid Isolation Kit

FACFK-100 100 preps
EUR 990

Detergent-Free Nuclei Isolation Kit

P524-2 - Ask for price

Detergent-Free Nuclei Isolation Kit

P524-20 - Ask for price

Detergent-Free Nuclei Isolation Kit

P524-80 - Ask for price

Mouse Brain Microvessel Isolation Kit

P750-4 - Ask for price

Broad Range Total RNA Isolation Kit

K1341050 1 kit
EUR 259

MagIso CD3 Cell Isolation Kit, Mouse

WHK-B009 20 tests, 200 tests Ask for price

Org Frontier? Chloroplast Isolation Kit

K468-10 each
EUR 666

MagIso CD90.2 Cell Isolation Kit, Mouse

WHK-B010 20 tests, 200 tests Ask for price

Easy3-Total NucleicAcid Isolation Kit

FYG504-100ML 100 ml Ask for price

Easy3-Total NucleicAcid Isolation Kit

FYG504-500ML 500 ml Ask for price

ExoPure? Isolation Kit   (Stem Cell Media)

K1239-10 each
EUR 901.2

ExoPure? Isolation Kit   (Stem Cell Media)

K1239-2 each
EUR 510

Exosome Isolation kit (for serum & plasma)

P101 - Ask for price

Exosome Isolation kit (for serum & plasma)

P101L - Ask for price

Exosome Isolation kit (for serum & plasma)

P101S - Ask for price

MagIso CX3CR1 Cell Isolation Kit, Mouse

WHK-B017 10 tests, 100 tests Ask for price

OrgFrontier? Plasma Membrane Isolation Kit

K414-10 each
EUR 679.2

MagIso CD4 T Cell Isolation Kit, Human

WHK-B001 10 tests
EUR 724.8

MagIso CD8 T Cell Isolation Kit, Human

WHK-B002 10 tests
EUR 724.8

MagIso CD4 T Cell Isolation Kit, Mouse

WHK-B003 10 tests
EUR 724.8

MagIso CD8 T Cell Isolation Kit, Mouse

WHK-B004 10 tests
EUR 724.8

MagIso Pan B Cell Isolation Kit, Mouse

WHK-B013 20 tests, 200 tests Ask for price

MagIso Pan B Cell Isolation Kit, Human

WHK-B022 20 tests, 200 tests Ask for price

Human Whole Blood Monocyte Isolation Kit

K933-30 each
EUR 451.2

EpiQuik Magbeads Quick RNA Isolation Kit

P-9106-050 50 samples
EUR 163.9

Soil Genomic DNA Kit

K1411-250 each
EUR 673.2

Soil Genomic DNA Kit

K1411-50 each
EUR 379.2

Exosome Isolation kit (forHuman Body Fluid)

P121 - Ask for price

Exosome Isolation kit (forHuman Body Fluid)

P121L - Ask for price

Exosome Isolation kit (forHuman Body Fluid)

P121S - Ask for price

Plant Genomic DNA Kit

20-abx098077
  • EUR 493.20
  • EUR 276.00
  • 200 rxns
  • 50 rxns

Micro Genomic DNA Kit

20-abx098242
  • EUR 126.00
  • EUR 393.60
  • 2 rxns
  • 50 rxns

Blood Genomic DNA Kit

abx098868-50rxns 50 rxns
EUR 326.4

Plant Genomic DNA Kit

abx294004-50preps 50 preps
EUR 477.6

Yeast Genomic DNA Kit

K1414-250 each
EUR 673.2

Yeast Genomic DNA Kit

K1414-50 each
EUR 379.2

To further explore the molecular mechanisms underlying the SCE therapy and determine the actions of CB-SC-release exosomes, we investigate phagocytizizes these exosomes during treatment with CB-SC. By co-cultivating DIO CB-SC-SC-labeled exosomes with human peripheral blood mononuclear cells (PBMC), we have found that CB-SC-derivative exosomes were mainly taken up by CD14-positive human monocytes, resulting in the Differentiation of monocytes in type 2 macrophages (M2), with morphology and expression resembling the spindle of molecular markers associated with M2.