Development of a CD63 Aptamer for Efficient Cancer Immunochemistry and Immunoaffinity-Based Exosome Isolation

CD63, members of Transmembrane-4-Superfamili Tetraspanin proteins and very n-glycosposomal membrane membrane proteins are known to regulate the malignancy of various types of cancer such as melanoma and breast cancer and function as potential markers for cancer detection. Recently, an important role as a classic exosome marker was also emphasized. In this work, through using a competitive Selex based magnetic beads (systematic evolution of ligands with exponential enrichment) and introducing NaCl 0.5m as an elution buffer, we identify two DNA aptamers (CD63-1 and CD63-2) with high affinity. and specificity for CD63 protein (KD = 38.71nm and 78.43, respectively).

Furthermore, CD63-1 was found efficiently in binding CD63 positive cells, including MDA-MB-231 cell breast cancer and too much HEK293T cells are expressed, with intermediate fastener affinity (KD ~ 100 Nm) as assessed by Flow cytometry. When the immunostaining test was carried out using clinical breast cancer biopsy, the CD63-1 APTIMER showed a comparable diagnostic efficacy for CD63 positive breast cancer with commercial antibodies. After developing the immunoaffinity imunoaffinity beads-based immunoaffinity system using Aptamer CD63-1, it was found that this bead-based system could effectively isolate the exosomes of MDA-MB-231 cell culture media and HT29.

Importantly, the introduction of Elution NaCl in this work allows the original exosom isolation through a simple 0.5m NaCl incubation step. Based on this result, we strongly believe that the aptamers developed can be useful for the exosom insulation of the original state efficiently from clinical samples and various terorostic applications for positive CD63 cancer.

Deposition of peptide affinity from extracellular vesicles and cell-free DNA improve sequencing performance for pathogenic mutation detection in plasma lung cancer patients

Liquid biopsy is a minimal-invasive diagnostic method that can increase access to molecular profiles for non-small patients lung cancer (NSCLC). Although the insulation of DNA (CF-DNA) cells from plasma is a standard liquid biopsy method to detect DNA mutations in cancer patients, its sensitivity can vary greatly. VN96 is a peptide with affinity for extracellular vesicles (EV) and circulating CF-DNA. In this study, we evaluated whether the Peptide-Affinity (PA) of EVS and CF-DNA precipitation of Plasma NSCLC patients increased the sensitivity of a single nucleotide variant (SNV) and compared with the observed SNV with those reported in suitable network biopsy.

Pope NSCLC patients are the target of PA precipitation or cell-free methods and total nucleic acids (TNA) are extracted; SNV is then detected by the next generation (NGS) sequencing. Pa causes an increase in DNA recovery and an increase in sequencing parameters of NGS when compared to CF-TNA. Reduced concordance with the network was observed in PA-TNA (62%) compared to CF-TNA (81%), mainly because of the identification of SNVs in Pa-TNA which were not observed in the network. The EGFR mutation was detected in PA-TNA with 83% sensitivity and 100% specificity.

In conclusion, PA-TNA can increase the abundant low allel detection limit using NGS. Stem Cell Educator Therapy (SCE) is a new clinical approach to the treatment of type 1 diabetes and other autoimmune diseases. SCE therapy circulates mononuclear blood cells isolated patients (for example, lymphocytes and monocytes) through apheresis machines, ko-culture mononuclear blood patients with stem cells that are reduced by the blood of adhered straps (CB-SC) on the SCE device, and then return ” Educated “these immune cells with patient blood. Exosom is a nano-sized extracellular vesicle between 30-150 nm in all biofluid media and cell culture.

 Development of a CD63 Aptamer for Efficient Cancer Immunochemistry and Immunoaffinity-Based Exosome Isolation
Development of a CD63 Aptamer for Efficient Cancer Immunochemistry and Immunoaffinity-Based Exosome Isolation

Clinical applications of extracellular vesicles of mesenchymal stem / stromal cells: therapeutic potential of an acellular product

Over the past decade, the sequery activity of mesenchymal stem cells (MSCS) has been widely studied due to its possible therapeutic role. In fact, MSCS releases extracellular vesicles (EVS) containing relevant biomolecules such as MRNAS, microarnas, bioactive lipids and signaling receptors, capable of restoring physiological conditions in which regenerative or anti-inflammatory actions are required. A real benefit would come from the therapeutic use of EV relative to MSC, avoiding possible immune release, pulmonary trapping, safety improvement and allowing crossing of organic barriers. A number of concerns must still be resolved concerning the mechanisms determining the beneficial effect of the MSC-EVS, the possible modification of their properties as a result of isolation / purification methods and / or the best approach for large production. scale for clinical use.

Most of the preclinical studies have been successful, reporting the role of MSC-EVS a role of protection in acute kidney injury after the opposing ischemia, powerful anti-inflammatory and anti-fibrotic effects by reducing inflammation Associated with diseases and fibrosis in lung and liver, as well as the modulation of innate and adaptive immune responses in transplant disease compared to host diseases (GVHD) as well as autoimmune diseases. However, the translation of MSC-EVS to the clinical stage is still in the initial phase. In this document, we discuss the therapeutic potential of an acellular product such as MSC derivative EVS (MSC-EVS) in acute and chronic pathologies.

Genomic DNA Isolation Kit

abx460025-96tests 96 tests
EUR 437.5

Genomic DNA Isolation Kit (Plant)

PDC10-0100 100 rxns
EUR 132

Genomic DNA Isolation Kit (Tissue)

PDC11-0100 100 rxns
EUR 132

Bacterial Genomic DNA Isolation Kit

K309-100 each
EUR 451.2

Genomic DNA isolation dual kit

PDC02-0100 100 rxns
EUR 110

Genomic DNA Isolation Reagent Kit

abx460021-1096tests 10 × 96 tests Ask for price

Genomic DNA Isolation Reagent Kit

abx460021-596tests 5 × 96 tests Ask for price

Genomic DNA Isolation Reagent Kit

abx460021-96tests 96 tests
EUR 325

Dual Genomic DNA Isolation Kit (Plant)

PDC05-0100 100 rxns
EUR 154

Dual Genomic DNA Isolation Kit(Tissue)

PDC06-0100 100 rxns
EUR 154

Genomic DNA Isolation Reagent

PDR01-0100 100 rxns
EUR 88

Rapid Fungal Genomic DNA Isolation Kit

FT71415 50Preps
EUR 91.97

Plant Tissue Genomic DNA Isolation Kit

K316-100 each
EUR 451.2

Easy3-Plant Genomic DNA Isolation Kit

FYG503-100P 100 Preps Ask for price

Easy3-Plant Genomic DNA Isolation Kit

FYG503-500P 500 Preps Ask for price

Rapid Bacteria Genomic DNA Isolation Kit

BS8225 50Preps
EUR 91.97

Mammalian Cell Genomic DNA Isolation Kit

K967-100 each
EUR 457.2

Plant Genomic DNA Isolation Reagent Kit

abx460022-1096tests 10 × 96 tests Ask for price

Plant Genomic DNA Isolation Reagent Kit

abx460022-596tests 5 × 96 tests Ask for price

Plant Genomic DNA Isolation Reagent Kit

abx460022-96tests 96 tests
EUR 325

One-Tube Bacteria Genomic DNA Isolation Kit

BS8413 100preps
EUR 94.72

One-Tube Bacteria Genomic DNA Isolation Kit

BS8414 500Preps, 500prep
EUR 198.85

Plant Genomic DNA Isolation Reagent

PDR02-0100 100 rxns
EUR 88

Column-Pure Animal Genomic DNA Isolation Kit

D512 50 Preparations
EUR 195

Column-Pure Bacterial Genomic DNA Isolation Kit

D511 50 Preparations
EUR 195

Genomic DNA Isolation Kit (Paraffin-embedded tissue)

PDC12-0100 100 rxns
EUR 132

Genomic DNA Isolation Kit (Blood/Cultured Cell/Fungus)

PDC09-0100 100 rxns
EUR 99

Magnetic Blood Genomic DNA Isolation Kit, 50 reactions/pk

208501 8 pcs/cs
EUR 74.91
Description: Magnetic Blood Genomic DNA Isolation Kit, 50 reactions/pk

Magnetic Blood Spots Genomic DNA Isolation Kit, 50 reactions/pk

208401 8 pcs/cs
EUR 74.91
Description: Magnetic Blood Spots Genomic DNA Isolation Kit, 50 reactions/pk

Genomic DNA Isolation Reagent Kit(Blood/Cultured Cell/Tissue)

PDR05-0100 100 rxns
EUR 154

DNA/RNA Mini Kit (isolation of both genomic DNA and total RNA from a single sample)

FADRK_050 1 kit
EUR 90

Genoirse Plant DNA Isolation Kit

GR101083 50
EUR 223

ISOLATE II Genomic DNA Kit

BIO-52065 10 preps Ask for price

ISOLATE II Genomic DNA Kit

BIO-52066 50 preps Ask for price

ISOLATE II Genomic DNA Kit

BIO-52067 250 preps Ask for price

Urine DNA Isolation Kit

K5011150 1 kit
EUR 261

Stool DNA Isolation Kit

K5011250 1 kit
EUR 261

Serum DNA Isolation Kit

K5018100 1 kit
EUR 391

Saliva DNA Isolation Kit

AG0226 Kit Ask for price
Description: Saliva DNA Isolation Kit provides a fast and simple spin column procedure for isolating high quality DNA from saliva samples collected and preserved using AcceGen's Saliva DNA Collection and Preservation Devices, as well as fresh saliva samples.Saliva DNA purified using AcceGen's kit is of the excellent quality, and is compatible with a number of downstream research applications including PCR, qPCR, Southern Blot analysis, sequencing and microarray analysis.

Saliva DNA Isolation Kit

K5011050 1 kit
EUR 261

Geneaid™ DNA Isolation Kit (Blood)

GEB003 each
EUR 3

Geneaid™ DNA Isolation Kit (Blood)

GEB01K each
EUR 450

Geneaid™ DNA Isolation Kit (Blood)

GEB100 each
EUR 75

Geneaid™ DNA Isolation Kit (Yeast)

GEY004 each
EUR 3

Geneaid™ DNA Isolation Kit (Yeast)

GEY100 each
EUR 64.5

Geneaid™ DNA Isolation Kit (Yeast)

GEY300 each
EUR 174

Geneaid™ DNA Isolation Kit (Tissue)

GET005 each
EUR 3

Geneaid™ DNA Isolation Kit (Tissue)

GET1.5K each
EUR 550

Geneaid™ DNA Isolation Kit (Tissue)

GET150 each
EUR 92.5

Mitochondrial DNA Isolation Kit

55R-1361 50 assays
EUR 660
Description: Mitochondrial DNA Isolation Kit for use in the research laboratory

Mitochondrial DNA Isolation Kit

GWB-AXR186 50 assays Ask for price

Mitochondrial DNA Isolation Kit

K2117-1 1 kit
EUR 686
Description: Isolates mt. DNA, no genomic DNA contamination

Mitochondrial DNA Isolation Kit

K2117-50 50 assays
EUR 718.8

Mitochondrial DNA Isolation Kit

K280-50 each
EUR 705.6

Geneaid™ DNA Isolation Kit (Bacteria)

GEE005 each
EUR 3

Geneaid™ DNA Isolation Kit (Bacteria)

GEE1.5K each
EUR 450

Geneaid™ DNA Isolation Kit (Bacteria)

GEE150 each
EUR 75

FitAmp Gel DNA Isolation Kit

P-1007 100 Samples
EUR 324.48

Plant/Fungi DNA Isolation Kit

10760114-1 50 Prep(s)
EUR 350.39

Whole Blood DNA Isolation Kit

K528-100 each
EUR 451.2

FitAmp Urine DNA Isolation Kit 

P-1017
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  • 100 Samples
  • 50 Samples
  • 100 Samples

XCF Exosomal DNA Isolation Kit

XCF200A-1 20 rxn
EUR 328

FastPure FFPE DNA Isolation Kit

DC105-01 50 rxns
EUR 75.2

Viral DNA/RNA Isolation Kit

AGDK-0044 Kit Ask for price
Description: The Viral DNA/RNA Isolation Kit is intended for rapid co-extraction of viral DNA and RNA from a variety of biofluid samples, such as, plasma, serum, milk and swap samples. The proprietary microspherical paramagnetic beads used in the kit have a large binding surfaces and a high affinity towards nucleic acids. Going through sample lysis/binding, washing, and elution steps, the whole process can be completed under 35 minutes, and yields highly pure nucleic acids elute.  The recovered nucleic acids can be used in a wide range of applications, such as PCR, RT-PCR, Sanger Sequencing, NGS, and gene chips.

Geneaid™ DNA Isolation Kit (Blood) Plus

GEB01K-A each
EUR 480

Geneaid™ DNA Isolation Kit (Tissue) Plus

GET1.5K-A each
EUR 594

Geneaid™ DNA Isolation Kit (Cultured Cell)

GEC005 each
EUR 3

Geneaid™ DNA Isolation Kit (Cultured Cell)

GEC1.5K each
EUR 360

Geneaid™ DNA Isolation Kit (Cultured Cell)

GEC150 each
EUR 60

Geneaid™ DNA Isolation Kit (Bacteria) Plus

GEE1.5K-A each
EUR 480

Blood and Serum DNA Isolation Kit

K5017100 1 kit
EUR 391

HipurA® Cotton DNA Isolation Kit

MB528-250PR 1 unit
EUR 213.83
Description: HipurA® Cotton DNA Isolation Kit

HipurA® Cotton DNA Isolation Kit

MB528-50PR 1 unit
EUR 47.85
Description: HipurA® Cotton DNA Isolation Kit

HiPurA® Banana DNA Isolation Kit

MB533-250PR 1 unit
EUR 182.59
Description: HiPurA® Banana DNA Isolation Kit

HiPurA® Banana DNA Isolation Kit

MB533-50PR 1 unit
EUR 46.63
Description: HiPurA® Banana DNA Isolation Kit

Apoptotic DNA Ladder Isolation Kit

GWB-AXR127 50 assays Ask for price

Apoptotic DNA Ladder Isolation Kit

K170-50 each
EUR 561.6

Apoptotic DNA Ladder Isolation Kit

K2045-50 50 assays
EUR 490
Description: No genomic DNA contamination.

FitAmp General Tissue Section DNA Isolation Kit 

P-1003
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  • Ask for price
  • Ask for price
  • 100 Samples
  • 50 Samples
  • 100 Samples

FitAmp Plasma/Serum DNA Isolation Kit 

P-1004
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  • Ask for price
  • Ask for price
  • 100 Samples
  • 50 Samples
  • 100 Samples

Geneaid™ DNA Isolation Kit (Cultured Cell) Plus

GEC1.5K-A each
EUR 400

HiPurA® SPP Blood DNA Isolation Kit

MB541-10ML 1 unit
EUR 27.19
Description: HiPurA® SPP Blood DNA Isolation Kit

HiPurA® SPP Blood DNA Isolation Kit

MB541-150ML 1 unit
EUR 155.06
Description: HiPurA® SPP Blood DNA Isolation Kit

HiPurA® SPP Blood DNA Isolation Kit

MB541-300ML 1 unit
EUR 242.51
Description: HiPurA® SPP Blood DNA Isolation Kit

HiPurA® SPP Blood DNA Isolation Kit

MB541-50ML 1 unit
EUR 68.71
Description: HiPurA® SPP Blood DNA Isolation Kit

Soil DNA Isolation Mini Kit (100prep)

FASOI-001-1 100 preps
EUR 241.2

Soil DNA Isolation Mini Kit (100prep)

FASOI_001-1 1 kit
EUR 90

Soil DNA Isolation Mini Kit (50prep)

FASOI-001 50 preps
EUR 189.6

Soil DNA Isolation Midi Kit (24prep)

FASOI-002 24 preps
EUR 259.2

Soil DNA Isolation Mini Kit (50prep)

FASOI_001 1 kit
EUR 45

FastPure FFPE DNA Isolation Mini Kit

DC105 50 rxn
EUR 241.2

FastPure Plant DNA Isolation Mini Kit

DC104 50 rxn
EUR 192

FastPure Plant DNA Isolation Mini Kit

DC104-01 50 rxns
EUR 60

HiPurA® Plant DNA Isolation Kit (CTAB Me

MB502-50PR 1 unit
EUR 33.57
Description: HiPurA® Plant DNA Isolation Kit (CTAB Me

FastPure Bacteria DNA Isolation Mini Kit

DC103 100 rxn
EUR 212.4

FastPure Bacteria DNA Isolation Mini Kit

DC103-01 100 rxns
EUR 52

HipurA® Silica Kit for DNA Isolation

MB503-400PR 1 unit
EUR 47.85
Description: HipurA® Silica Kit for DNA Isolation

FitAmp Paraffin Tissue Section DNA Isolation Kit 

P-1009
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  • Ask for price
  • 100 Samples
  • 50 Samples
  • 100 Samples

EpiQuik Circulating Cell-Free DNA Isolation Kit

P-1064
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  • Ask for price
  • Ask for price
  • 50 Isolations
  • 25 Isolations
  • 50 Isolations

Mitochondrial DNA Isolation Kit, Catalog: PI-0105

PI-0105 50 isolations
EUR 465
Description: To isolate intact mitochondrial DNA from a variety of cells and tissue types without contamination from genomic DNA. 

FastPure Blood DNA Isolation Mini Kit V2

DC111-01 50 rxn
EUR 214.8

FastPure Blood DNA Isolation Mini Kit V2

DC111-02 200 rxn
EUR 472.8

Soil DNA Plus Isolation Mini Kit (100prep)

FAPSO-001-1 100 preps
EUR 241.2

FastPure Cell/Tissue DNA Isolation Mini Kit

DC102 100 rxn
EUR 212.4

FastPure Cell/Tissue DNA Isolation Mini Kit

DC102-01 100 rxns
EUR 52

Plasmid DNA Isolation Solution I

10760028-1 250 mL
EUR 29.83

Plasmid DNA Isolation Solution I

10760028-2 500 mL
EUR 45.3

Plasmid DNA Isolation Solution II

10760029-1 250 mL
EUR 29.83

Plasmid DNA Isolation Solution II

10760029-2 500 mL
EUR 37.89

Soil DNA Plus Isolation Mini Kit (50prep)

FAPSO-001 50 preps
EUR 189.6

Methylamp Coupled DNA Isolation & Modification Kit

P-1002 40 Samples
EUR 474.12

Plasmid DNA Isolation Solution III

10760030-1 250 mL
EUR 28.07

Plasmid DNA Isolation Solution III

10760030-2 500 mL
EUR 43

QC Buffer for Plasmid DNA Isolation

10760036-1 250 mL
EUR 25.45

QC Buffer for Plasmid DNA Isolation

10760036-2 500 mL
EUR 43.01

QF Buffer for Plasmid DNA Isolation

10760037-1 250 mL
EUR 24.44

QF Buffer for Plasmid DNA Isolation

10760037-2 500 mL
EUR 43.01

QF Buffer for Plasmid DNA Isolation

10760037-3 1 L
EUR 76.87

One Tube Viral DNA Isolation Buffer

VT4785 50Preps
EUR 224.44

One Tube Viral DNA Isolation Buffer

VT4786 100preps
EUR 388.86

Magnetic Cell-Free DNA Isolation Kit, 50 reactions/pk

208301 6 pcs/cs
EUR 183.37
Description: Magnetic Cell-Free DNA Isolation Kit, 50 reactions/pk

EpiMag High Throughput DNA Isolation Universal Kit

P-1022-96 96 Samples
EUR 227.7

EpiMag High Throughput DNA Isolation Universal Kit

P-1022 96 Samples
EUR 215

QBT Buffer for Plasmid DNA Isolation

10760035-1 250 mL
EUR 34.22

QBT Buffer for Plasmid DNA Isolation

10760035-2 500 mL
EUR 49.13

QBT Buffer for Plasmid DNA Isolation

10760035-3 1 L
EUR 71.93

Hipro Circulating Cell-Free DNA (cfDNA) Isolation Kit

W2603-10 10 rxn
EUR 319

Hipro Circulating Cell-Free DNA (cfDNA) Isolation Kit

W2603-20 20 rxn
EUR 499

Hipro Circulating Cell-Free DNA (cfDNA) Isolation Kit

W2603-50 - Ask for price

Plasmid DNA Isolation Solutions I, II, III

10760027-1 1 Kit
EUR 85.98

miRNA Isolation Kit

FYG304-100P 100 Preps Ask for price

miRNA Isolation Kit

FAMIK--001 Rxns: 100
EUR 398.4
Description: Contents of the kit:
  • Rxns:100Lysis
  • Buffer-10 ml
  • Acid-Phenol-10 ml
  • RNA Column and Collection Tube-100 sets
  • Wash Buffer-10 ml
  • Release Buffer-0.5 ml

miRNA Isolation Kit

FAMIK--002 Rxns: 50
EUR 267.6

Exosome Isolation Kit

E601E001 20 tests
EUR 2500

MicroRNA Isolation Kit

KS341025 1 kit
EUR 284

Endosome Isolation Kit

P528 - Ask for price

Microsome Isolation Kit

K249-50 each
EUR 633.6

Protoplast Isolation Kit

30210002-1 20 Prep(s)
EUR 199.16

Protoplast Isolation Kit

30210002-2 50 Prep(s)
EUR 438.32

FastPure Blood/Cell/Tissue/Bacteria DNA Isolation Mini Kit

DC112-01 50 rxns
EUR 63.6

FastPure Blood/Cell/Tissue/Bacteria DNA Isolation Mini Kit

DC112-02 200 rxns
EUR 223.2

Chloroplast Isolation Kit

30210000-1 20 Prep(s)
EUR 273.97

Rna Isolation Kit - EACH

11828665001 EACH
EUR 425.95

Mitochondria Isolation Kit

AR0156 1 kit
EUR 127.2

Preadipocyte Isolation Kit

K583-5 each
EUR 639.6

Total RNA Isolation Kit

P-9100 50 samples
EUR 416.7

To further explore the molecular mechanisms underlying the SCE therapy and determine the actions of CB-SC-release exosomes, we investigate phagocytizizes these exosomes during treatment with CB-SC. By co-cultivating DIO CB-SC-SC-labeled exosomes with human peripheral blood mononuclear cells (PBMC), we have found that CB-SC-derivative exosomes were mainly taken up by CD14-positive human monocytes, resulting in the Differentiation of monocytes in type 2 macrophages (M2), with morphology and expression resembling the spindle of molecular markers associated with M2.