Umbilical Cord Mesenchymal Stem Cells as well as their Released Exosomes Suppress Proliferation of Activated PBMCs in Multiple Sclerosis

Multiple Sclerosis (MS) is a degenerative disorder of the central nervous system (CNS) caused by an attack mediated by autoimmune against Mielin protein. Previously, mesenchymal stem cells were considered novel and successful treatment for MS. One of the underlying mechanisms behind its immunomodulator function is the release of extracellular vesicles, especially exosom. In this study, we aim to evaluate the efficacy of their MSCS and exosom suppression at the proliferation of peripheral mononuclear blood cells (PBMC) in MS (RRMS) patients who relapse and healthy subjects.

What must be done, mesenchymal stem cells come from human navel cord tissue and are used for exosomous insulation through ultracentrification. The MSCS and Exosom oppression functions which are revealed MSC are checked in pain with PBMC labeled CFSE in vitro. PBMC proliferation of patients and healthy individuals is measured using flow chicometry. We first showed that the proliferation of PBMC declined in the presence of MSC and the oppression was more efficient by the exosomes derived by MSC, with the minimum alloreax level. However, their MSCS and exosom suppression capacity decreased significantly during broad sub-cultivation. This study indicates that the exosomes derived MSC as cell-free therapy can effectively prevent the proliferation of PBMC. However, further evaluation needs to move towards a functional approach that can be translated into the clinic. The drug delivery system that is reduced by cells (DDSS), especially exosom, has grown in popularity and is increasingly explored as a novel DDS, because of their intrinsic targeting capabilities.

However, the clinical translation of the exosomes is required by boring insulation procedures and poor results. The recent nanovesicle cell (CDN) cell (CDN) was produced and proposed as a mimetic of exosomes. Various methods for producing exosoms-mimethics have been developed. In this chapter, we present a simple, efficient, and cost-effective CDN production method that uses general laboratory equipment (microcentrifuge) and Spin Cup. Through a series of extrusion steps and exclusion sizes, CDN is produced from in vitro cell culture and is found very resembling endogenous exosom. Thus, we imagine that this strategy has great potential as a proper alternative to exosom in the development of ideal DDS.

Comparison of excosom refining methods using the Marek (MDV) virus serum (MDV) -vaccinated and -The chicken bearings

Marek (MD) disease is the Alphaherpesvirus (Marek, MDV) virus -Induced chicken pathology associated with paralysis, immunosuppression, neurological signs, and t lymphoma. MD is controlled in the production of poultry through a direct attenuated vaccine. The purpose of this study was to compare the method for triggering exosomes of chicken serum (VEX) who were vaccinated and protected and chicken tumors (Tex) for biomarker analysis of the protection induced by vaccines and MD lymphoma respectively.

The standard method of polyethylene glycol (PEG, 8%) compared to commercial reagents (total insulation reagent exosome, TEI) for excosomal content and RNA content. Although the exosomes are purified by PEG or TEI comparable in size and morphology, Te-reagents produce a greater 3-4 times concentration. Relative expression 8 of the 10 G. Gallus- and MDV1-MIRNAs encoded examined significant differences depending on the precipitation method used. Peg standards produce comparable, although lower than the exosomes than TEI reagents and distinctive Minna compositions.

 Umbilical Cord Mesenchymal Stem Cells as well as their Released Exosomes Suppress Proliferation of Activated PBMCs in Multiple Sclerosis
Umbilical Cord Mesenchymal Stem Cells as well as their Released Exosomes Suppress Proliferation of Activated PBMCs in Multiple Sclerosis

Analysis of non-annotated and unannotified non-coded RNAs of the subtypes of exosome using a new generation RNA sequencing

Non-coding RNAs (LNCRNAS) contain> 200 nucleotides and act as regulatory molecules in transcription and translation processes under normal and pathological conditions. LNCRNAS have been reported to locate in nuclei, cytoplasm and, more recently, extracellular vesicles such as exosomes. The exosomal LNCRAS have gained great attention because the secreted exosomes of a cell type can transfer their cargo (for example, their cargo, protein species, RNA species and lipids) to the recipient cells and modifications. Phenotypic mediates in the recipient cell. In recent years, many exosomal lncnas have been discovered and annotated and attracts a lot of attention as potential markers for the diagnosis and prognosis of diseases. Numerous exosomal lncnas are expected to be identified. However, the characterization of non-annual exosomal RNAs with non-protein coding sequences from massive RNA sequencing data is technically difficult. Here we describe a method for the discovery of annotated and unspoastly exosomal LNCRNO.

This method includes a large-scale and purification isolation strategy for exosomatic subtypes, using the human colorectal cancer (LIM1863) cell line as a model. RNA sequencing method inputs read and performs a transcription set to identify annotated and flooded exosomal LNCRAS. Cuts (length, number of exon, classification code and probability of coding of human proteins) are used to potentially identify new exosomal lncnas. The calculation of the read read reading and differential expression analysis are also introduced for downstream analysis and selection of candidates. The exosomal candidates of LNCRNA are validated using RT-QPCR. This method provides a model for the discovery and analysis of the exosomal LNCRNA from the new generation RNA sequencing.

Stool DNA Purification Kit

K1474-50 50 Preps
EUR 417.6

FavorPrep Stool DNA Plus Isolation Mini Kit (50prep), with Proteinase K Powder

FAPST001 50 preps
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FavorPrep Stool DNA Plus Isolation Mini Kit (100prep), with Proteinase K Powder

FAPST001-1 100 preps
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Mitochondrial DNA Isolation Kit

55R-1361 50 assays
EUR 990
Description: Mitochondrial DNA Isolation Kit for use in the research laboratory

Genomic DNA Isolation Kit

55R-1362 50 assays
EUR 462
Description: Genomic DNA Isolation Kit for use in the research laboratory

Mitochondrial DNA Isolation Kit

K2117-50 50 assays
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Genomic DNA Isolation Kit

K2118-50 50 assays
EUR 385.2

Saliva DNA Isolation Kit

K5011050 1 kit
EUR 307.2

Urine DNA Isolation Kit

K5011150 1 kit
EUR 307.2

Serum DNA Isolation Kit

K5018100 1 kit
EUR 432

Mitochondrial DNA Isolation Kit

K280-50
EUR 705.6

Genomic DNA Isolation Kit

K281-50
EUR 326.4

Genorise® Stool DNA Extraction Kit-1000

GR101086 1000
EUR 1008

Genorise® Stool DNA Extraction Kit-100

GR101086-100 100
EUR 172.8

Genorise® Stool DNA Extraction Kit-200

GR101086-200 200
EUR 309.6

Genorise® Stool DNA Extraction Kit-50

GR101086-50 50
EUR 104.4

Genorise® Stool DNA Extraction Kit-500

GR101086-500 500
EUR 576

Apoptotic DNA Ladder Isolation Kit

K170-50
EUR 561.6

Genoirse Plant DNA Isolation Kit

GR101083 50
EUR 267.6

Apoptotic DNA Ladder Isolation Kit

K2045-50 50 assays
EUR 602.4

Whole Blood DNA Isolation Kit

K528-100
EUR 451.2

Bacterial Genomic DNA Isolation Kit

K309-100
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XCF Exosomal DNA Isolation Kit

XCF200A-1 20 rxn
EUR 463.2

FitAmp Gel DNA Isolation Kit

P-1007 100 Samples
EUR 324.48

FitAmp Urine DNA Isolation Kit 

P-1017
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FitAmp Urine DNA Isolation Kit 

P-1017-050 50 Samples
EUR 171.6

FitAmp Urine DNA Isolation Kit 

P-1017-100 100 Samples
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100 BP DNA LADDER, 500UL PER KIT

M-DNA-100BP 1/pk
EUR 87.6
Description: Bioscience Mol Bio; DNA Ladder

1 KB DNA LADDER, 500UL PER KIT

M-DNA-1KB 1/pk
EUR 84
Description: Bioscience Mol Bio; DNA Ladder

Genorise® Stool DNA Purification Mini Kit 50

GR101082 50
EUR 177.6

Genorise® Stool DNA Purification Mini Kit-100

GR101082-100 100
EUR 320.4

Genorise® Stool DNA Purification Mini Kit-200

GR101082-200 200
EUR 572.4

Genorise® Stool DNA Purification Mini Kit-500

GR101082-500 500
EUR 1288.8

FastPure Bacteria DNA Isolation Mini Kit

DC103 100 rxn
EUR 212.4

FastPure Plant DNA Isolation Mini Kit

DC104 50 rxn
EUR 192

FastPure FFPE DNA Isolation Mini Kit

DC105 50 rxn
EUR 241.2

Rapid Bacteria Genomic DNA Isolation Kit

BS8225 50Preps
EUR 91.97

Mammalian Cell Genomic DNA Isolation Kit

K967-100
EUR 457.2

Rapid Fungal Genomic DNA Isolation Kit

FT71415 50Preps
EUR 91.97

Easy3-Plant Genomic DNA Isolation Kit

FYG503-100P 100 Preps Ask for price

Easy3-Plant Genomic DNA Isolation Kit

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Soil DNA Isolation Mini Kit (50prep)

FASOI-001 50 preps
EUR 189.6

Soil DNA Isolation Mini Kit (100prep)

FASOI-001-1 100 preps
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Soil DNA Isolation Midi Kit (24prep)

FASOI-002 24 preps
EUR 259.2

Blood and Serum DNA Isolation Kit

K5017100 1 kit
EUR 432

Plant Tissue Genomic DNA Isolation Kit

K316-100
EUR 451.2

Methylamp Coupled DNA Isolation & Modification Kit

P-1002 40 Samples
EUR 474.12

FitAmp Plasma/Serum DNA Isolation Kit 

P-1004
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FitAmp Plasma/Serum DNA Isolation Kit 

P-1004-1 50 Samples
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FitAmp Plasma/Serum DNA Isolation Kit 

P-1004-2 100 Samples
EUR 253

FastPure Cell/Tissue DNA Isolation Mini Kit

DC102 100 rxn
EUR 212.4

FastPure Blood DNA Isolation Mini Kit V2

DC111-01 50 rxn
EUR 214.8

FastPure Blood DNA Isolation Mini Kit V2

DC111-02 200 rxn
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One-Tube Bacteria Genomic DNA Isolation Kit

BS8413 100preps
EUR 94.72

One-Tube Bacteria Genomic DNA Isolation Kit

BS8414 500Preps, 500prep
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Soil DNA Plus Isolation Mini Kit (50prep)

FAPSO-001 50 preps
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Soil DNA Plus Isolation Mini Kit (100prep)

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FitAmp General Tissue Section DNA Isolation Kit 

P-1003
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FitAmp Paraffin Tissue Section DNA Isolation Kit 

P-1009
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EpiMag High Throughput DNA Isolation Universal Kit

P-1022-96 96 Samples
EUR 227.7

EpiQuik Circulating Cell-Free DNA Isolation Kit

P-1064
  • EUR 416.70
  • EUR 148.50
  • EUR 253.00
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FitAmp General Tissue Section DNA Isolation Kit 

P-1003-1 50 Samples
EUR 253

FitAmp General Tissue Section DNA Isolation Kit 

P-1003-2 100 Samples
EUR 490.6

FitAmp Paraffin Tissue Section DNA Isolation Kit 

P-1009-1 50 Samples
EUR 239.8

FitAmp Paraffin Tissue Section DNA Isolation Kit 

P-1009-2 100 Samples
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EpiQuik Circulating Cell-Free DNA Isolation Kit

P-1064-25 25 Isolations
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EpiQuik Circulating Cell-Free DNA Isolation Kit

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One Tube Viral DNA Isolation Buffer

VT4785 50Preps
EUR 224.44

One Tube Viral DNA Isolation Buffer

VT4786 100preps
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Hipro Circulating Cell-Free DNA (cfDNA) Isolation Kit

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EpiQuik Circulating Cell-Free DNA (ccfDNA) Isolation Easy Kit

P-1065
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EpiQuik Circulating Cell-Free DNA (ccfDNA) Isolation Easy Kit

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EpiQuik Circulating Cell-Free DNA (ccfDNA) Isolation Easy Kit

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EUR 253

Mitochondria Isolation Kit

AR0156 1 kit
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MicroRNA Isolation Kit

KS341025 1 kit
EUR 307.2
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

miRNA Isolation Kit

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miRNA Isolation Kit

FAMIK--001 Rxns: 100
EUR 398.4
Description: Contents of the kit:
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  • Acid-Phenol-10 ml
  • RNA Column and Collection Tube-100 sets
  • Wash Buffer-10 ml
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miRNA Isolation Kit

FAMIK--002 Rxns: 50
EUR 267.6

Preadipocyte Isolation Kit

K583-5
EUR 639.6

Microsome Isolation Kit

K249-50
EUR 633.6

Endosome Isolation Kit

P528 - Ask for price

Rna Isolation Kit

11828665001 EACH
EUR 330.6

H. Pylori Ag Stool Extraction Kit

DEIA1951 50T
EUR 760.8
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Apoptotic Cell Isolation Kit

55R-1349 30 units
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Description: Apoptotic Cell Isolation Kit for use in the research laboratory

Cell Mitochondria Isolation Kit

abx090630-50100tests 50-100 tests
EUR 309.6

Tissue Mitochondria Isolation Kit

abx090631-50100assays 50-100 assays
EUR 309.6

Lipid Droplet Isolation Kit

MET-5011 50 preps
EUR 498
Description: Lipid droplets are lipid rich organelles found in the adipose tissue of eukaryotes. They function to regulate the hydrolysis and storage of neutral lipids and serve as storage for cholesterol and acyl-glycerols. Lipid droplets have also been associated with inflammatory responses, obesity, atherosclerosis, and cancer.

ExoPure? Isolation Kit   (Urine)

K1240-10
EUR 836.4

ExoPure? Isolation Kit   (Urine)

K1240-2
EUR 483.6

Easy3-RNA Isolation Kit

FYG501-100P 100 Preps Ask for price

Easy3-RNA Isolation Kit

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Cartilage RNA Isolation Kit

K2031010 1 kit
EUR 502.8
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Lipopolysaccharide (LPS) Isolation Kit

K438-10
EUR 333.6

Apoptotic Cell Isolation Kit

K258-30
EUR 561.6

Yeast Mitochondria Isolation Kit

K259-50
EUR 502.8

Yeast Nuclei Isolation Kit

K289-50
EUR 470.4

Total RNA Isolation Kit

P-9100 50 samples
EUR 416.7

Soil/ Stool RNA Extraction MicroElute Kit (100prep)

FAFRK-001-MICRO 100 preps
EUR 363.6

Labsit Stool Foam w Castors

FUR3548 EACH
EUR 158.46

Stool Stacking 610mm Night Grey

FUR5050 EACH
EUR 86.64

Stool Stacking 660mm Night Grey

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EUR 86.64

Frit Kit

FRIT-KIT 1each
EUR 148.8
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Column Packing Kit

PACK-KIT 1pack
EUR 1242
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

Rapid Blood RNA Isolation Kit

BT4182 10Preps
EUR 98.36

Rapid Blood RNA Isolation Kit

BT4183 50Preps
EUR 197.03

Rapid Blood RNA Isolation Kit

BT4184 250preps
EUR 608.1

Rapid Bacteria RNA Isolation Kit

BS8625 50Preps
EUR 95.63

Exosome Isolation kit (for urine)

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Exosome Isolation kit (for urine)

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EZExtract? Core Histone Isolation Kit

K234-100
EUR 314.4

ExoPure? Isolation Kit   (Cell Media)

K1237-10
EUR 796.8

ExoPure? Isolation Kit   (Cell Media)

K1237-2
EUR 470.4

ExoPure? Isolation Kit   (Serum, Plasma)

K1238-10
EUR 836.4

ExoPure? Isolation Kit   (Serum, Plasma)

K1238-2
EUR 470.4

ExoPure? Isolation Kit   (Bio Fluids)

K1241-10
EUR 836.4

ExoPure? Isolation Kit   (Bio Fluids)

K1241-2
EUR 483.6

Exo-Urine EV Isolation Kit

EXOU100A-1 2 x 5 reactions
EUR 661.2

Easy3-Plant RNA Isolation Kit

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Easy3-Plant RNA Isolation Kit

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Easy3-Total NucleicAcid Isolation Kit

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Easy3-Total NucleicAcid Isolation Kit

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Circulating Nuleic Acid Isolation Kit

FACFK-050 50 preps
EUR 589.2

Circulating Nuleic Acid Isolation Kit

FACFK-100 100 preps
EUR 990

Total RNA Isolation Kit II

FATRS--001 100 preps
EUR 369.6

Total RNA Isolation Kit II

FATRS--050 50 preps
EUR 259.2

Org Frontier? Chloroplast Isolation Kit

K468-10
EUR 666

OrgFrontier? Plasma Membrane Isolation Kit

K414-10
EUR 679.2

Cell Surface Protein Isolation Kit

K295-10
EUR 601.2

Detergent-Free Nuclei Isolation Kit

P524-2 - Ask for price

Detergent-Free Nuclei Isolation Kit

P524-20 - Ask for price

Detergent-Free Nuclei Isolation Kit

P524-80 - Ask for price

Lysosome Isolation Kit (20 tests)

P534 - Ask for price

Mouse Brain Microvessel Isolation Kit

P750-4 - Ask for price

Rapid Plant RNA Isolation Kit

PT4191 50Preps
EUR 95.63

ChromaFlash Chromatin Isolation & Shearing Kit 

P-2023
  • EUR 324.48
  • EUR 189.20
  • 100 Preperations
  • 100 Preparations

ChromaFlash Chromatin Isolation & Shearing Kit 

P-2023-100 100 Preparations
EUR 189.2

Total RNA Isolation Kit (Tissue)

P-9102 50 samples
EUR 416.7

Total RNA Isolation Kit (Plant)

P-9104 50 samples
EUR 416.7

EpiMag Viral RNA Isolation Kit

P-9108-050 50 samples
EUR 152.9

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 319.2

NATtrol Rotavirus (Stool Matrix) (0.5 mL)

NATROTA-GP 0.5 mL
EUR 112.42
Description: Please contact Gentaur in order to receive the datasheet of the product.

PSEUDOMONAS ISOLATION AGAR

P16-114-10kg 10 kg
EUR 2058

PSEUDOMONAS ISOLATION AGAR

P16-114-2kg 2kg
EUR 494.4

PSEUDOMONAS ISOLATION AGAR

P16-114-500g 500 g
EUR 177.6

PSEUDOMONAS ISOLATION BROTH

P16-115-10kg 10 kg Ask for price

PSEUDOMONAS ISOLATION BROTH

P16-115-2Kg 2 Kg Ask for price

PSEUDOMONAS ISOLATION BROTH

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Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit

CAS400A-KIT 1 kit (10 rxn)
EUR 1332

Pesticide Kit Containing All 10 Multi-Compound Mixes

SPXPR-KIT 1ML
EUR 1923.18

CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV100PA-KIT 1 Kit
EUR 1358.4

The current hybrid hybrid is not easily dissociated into the biological buffers, which is thus suitable for use in “liquid biopsy”, requiring a long-term incubation process with biological samples. In addition, it is gradually degraded by the action of chelating agents; An effortless recovery of traffic markers has been performed. Then we modified the spheres of the CSID hydrogel with the anti-EPCAM antibody (“immunosphere CSID”) and the anti-CD63 antibody to isolate two promising circulation markers in the biopsy Liquid: CTC and CTXS. The capacities of the immunospheres for the isolation of markers and recovery have been confirmed by a fluorescence image, where the spheres isolate and effortlessly recover the target traffic markers.