Umbilical Cord Mesenchymal Stem Cells as well as their Released Exosomes Suppress Proliferation of Activated PBMCs in Multiple Sclerosis

Multiple Sclerosis (MS) is a degenerative disorder of the central nervous system (CNS) caused by an attack mediated by autoimmune against Mielin protein. Previously, mesenchymal stem cells were considered novel and successful treatment for MS. One of the underlying mechanisms behind its immunomodulator function is the release of extracellular vesicles, especially exosom. In this study, we aim to evaluate the efficacy of their MSCS and exosom suppression at the proliferation of peripheral mononuclear blood cells (PBMC) in MS (RRMS) patients who relapse and healthy subjects.

What must be done, mesenchymal stem cells come from human navel cord tissue and are used for exosomous insulation through ultracentrification. The MSCS and Exosom oppression functions which are revealed MSC are checked in pain with PBMC labeled CFSE in vitro. PBMC proliferation of patients and healthy individuals is measured using flow chicometry. We first showed that the proliferation of PBMC declined in the presence of MSC and the oppression was more efficient by the exosomes derived by MSC, with the minimum alloreax level. However, their MSCS and exosom suppression capacity decreased significantly during broad sub-cultivation. This study indicates that the exosomes derived MSC as cell-free therapy can effectively prevent the proliferation of PBMC. However, further evaluation needs to move towards a functional approach that can be translated into the clinic. The drug delivery system that is reduced by cells (DDSS), especially exosom, has grown in popularity and is increasingly explored as a novel DDS, because of their intrinsic targeting capabilities.

However, the clinical translation of the exosomes is required by boring insulation procedures and poor results. The recent nanovesicle cell (CDN) cell (CDN) was produced and proposed as a mimetic of exosomes. Various methods for producing exosoms-mimethics have been developed. In this chapter, we present a simple, efficient, and cost-effective CDN production method that uses general laboratory equipment (microcentrifuge) and Spin Cup. Through a series of extrusion steps and exclusion sizes, CDN is produced from in vitro cell culture and is found very resembling endogenous exosom. Thus, we imagine that this strategy has great potential as a proper alternative to exosom in the development of ideal DDS.

Comparison of excosom refining methods using the Marek (MDV) virus serum (MDV) -vaccinated and -The chicken bearings

Marek (MD) disease is the Alphaherpesvirus (Marek, MDV) virus -Induced chicken pathology associated with paralysis, immunosuppression, neurological signs, and t lymphoma. MD is controlled in the production of poultry through a direct attenuated vaccine. The purpose of this study was to compare the method for triggering exosomes of chicken serum (VEX) who were vaccinated and protected and chicken tumors (Tex) for biomarker analysis of the protection induced by vaccines and MD lymphoma respectively.

The standard method of polyethylene glycol (PEG, 8%) compared to commercial reagents (total insulation reagent exosome, TEI) for excosomal content and RNA content. Although the exosomes are purified by PEG or TEI comparable in size and morphology, Te-reagents produce a greater 3-4 times concentration. Relative expression 8 of the 10 G. Gallus- and MDV1-MIRNAs encoded examined significant differences depending on the precipitation method used. Peg standards produce comparable, although lower than the exosomes than TEI reagents and distinctive Minna compositions.

 Umbilical Cord Mesenchymal Stem Cells as well as their Released Exosomes Suppress Proliferation of Activated PBMCs in Multiple Sclerosis
Umbilical Cord Mesenchymal Stem Cells as well as their Released Exosomes Suppress Proliferation of Activated PBMCs in Multiple Sclerosis

Analysis of non-annotated and unannotified non-coded RNAs of the subtypes of exosome using a new generation RNA sequencing

Non-coding RNAs (LNCRNAS) contain> 200 nucleotides and act as regulatory molecules in transcription and translation processes under normal and pathological conditions. LNCRNAS have been reported to locate in nuclei, cytoplasm and, more recently, extracellular vesicles such as exosomes. The exosomal LNCRAS have gained great attention because the secreted exosomes of a cell type can transfer their cargo (for example, their cargo, protein species, RNA species and lipids) to the recipient cells and modifications. Phenotypic mediates in the recipient cell. In recent years, many exosomal lncnas have been discovered and annotated and attracts a lot of attention as potential markers for the diagnosis and prognosis of diseases. Numerous exosomal lncnas are expected to be identified. However, the characterization of non-annual exosomal RNAs with non-protein coding sequences from massive RNA sequencing data is technically difficult. Here we describe a method for the discovery of annotated and unspoastly exosomal LNCRNO.

This method includes a large-scale and purification isolation strategy for exosomatic subtypes, using the human colorectal cancer (LIM1863) cell line as a model. RNA sequencing method inputs read and performs a transcription set to identify annotated and flooded exosomal LNCRAS. Cuts (length, number of exon, classification code and probability of coding of human proteins) are used to potentially identify new exosomal lncnas. The calculation of the read read reading and differential expression analysis are also introduced for downstream analysis and selection of candidates. The exosomal candidates of LNCRNA are validated using RT-QPCR. This method provides a model for the discovery and analysis of the exosomal LNCRNA from the new generation RNA sequencing.

FavorPrep Stool DNA Plus Isolation Mini Kit (50prep), with Proteinase K Powder

FAPST001 50 preps
EUR 198

Urine DNA Isolation Kit

K5011150 1 kit
EUR 237

Serum DNA Isolation Kit

K5018100 1 kit
EUR 355

Saliva DNA Isolation Kit

K5011050 1 kit
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Genomic DNA Isolation Kit

55R-1362 50 assays
EUR 462
Description: Genomic DNA Isolation Kit for use in the research laboratory

Genomic DNA Isolation Kit

K281-50 each
EUR 326.4

Genomic DNA Isolation Kit

K2118-50 50 assays
EUR 385.2

Mitochondrial DNA Isolation Kit

55R-1361 50 assays
EUR 990
Description: Mitochondrial DNA Isolation Kit for use in the research laboratory

Mitochondrial DNA Isolation Kit

K280-50 each
EUR 705.6

Mitochondrial DNA Isolation Kit

K2117-50 50 assays
EUR 718.8

Mitochondrial DNA Isolation Kit

K2117-1 1 kit
EUR 858
Description: Isolates mt. DNA, no genomic DNA contamination

FitAmp Gel DNA Isolation Kit

P-1007 100 Samples
EUR 324.48

Whole Blood DNA Isolation Kit

K528-100 each
EUR 451.2

Plant/Fungi DNA Isolation Kit

10760114-1 50 Prep(s)
EUR 350.39

FitAmp Urine DNA Isolation Kit 

P-1017
  • EUR 474.12
  • EUR 171.60
  • EUR 292.60
  • 100 Samples
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  • 100 Samples

XCF Exosomal DNA Isolation Kit

XCF200A-1 20 rxn
EUR 328

FastPure FFPE DNA Isolation Kit

DC105-01 50 rxns
EUR 75.2

Genoirse Plant DNA Isolation Kit

GR101083 50
EUR 223

Bacterial Genomic DNA Isolation Kit

K309-100 each
EUR 451.2

Blood and Serum DNA Isolation Kit

K5017100 1 kit
EUR 355

Apoptotic DNA Ladder Isolation Kit

K170-50 each
EUR 561.6

Apoptotic DNA Ladder Isolation Kit

K2045-50 50 assays
EUR 602.4

FitAmp Plasma/Serum DNA Isolation Kit 

P-1004
  • EUR 416.70
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  • EUR 253.00
  • 100 Samples
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  • 100 Samples

Rapid Fungal Genomic DNA Isolation Kit

FT71415 50Preps
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Plant Tissue Genomic DNA Isolation Kit

K316-100 each
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Easy3-Plant Genomic DNA Isolation Kit

FYG503-100P 100 Preps Ask for price

Easy3-Plant Genomic DNA Isolation Kit

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Rapid Bacteria Genomic DNA Isolation Kit

BS8225 50Preps
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Mammalian Cell Genomic DNA Isolation Kit

K967-100 each
EUR 457.2

Soil DNA Isolation Mini Kit (100prep)

FASOI-001-1 100 preps
EUR 241.2

Soil DNA Isolation Mini Kit (100prep)

FASOI_001-1 1 kit
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Soil DNA Isolation Mini Kit (50prep)

FASOI-001 50 preps
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Soil DNA Isolation Midi Kit (24prep)

FASOI-002 24 preps
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Soil DNA Isolation Mini Kit (50prep)

FASOI_001 1 kit
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FastPure FFPE DNA Isolation Mini Kit

DC105 50 rxn
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FastPure Plant DNA Isolation Mini Kit

DC104 50 rxn
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FastPure Plant DNA Isolation Mini Kit

DC104-01 50 rxns
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One-Tube Bacteria Genomic DNA Isolation Kit

BS8413 100preps
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One-Tube Bacteria Genomic DNA Isolation Kit

BS8414 500Preps, 500prep
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FastPure Bacteria DNA Isolation Mini Kit

DC103 100 rxn
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FastPure Bacteria DNA Isolation Mini Kit

DC103-01 100 rxns
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FitAmp General Tissue Section DNA Isolation Kit 

P-1003
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FitAmp Paraffin Tissue Section DNA Isolation Kit 

P-1009
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  • EUR 454.30
  • 100 Samples
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  • 100 Samples

FastPure Blood DNA Isolation Mini Kit V2

DC111-01 50 rxn
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FastPure Blood DNA Isolation Mini Kit V2

DC111-02 200 rxn
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Plasmid DNA Isolation Solution I

10760028-1 250 mL
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Plasmid DNA Isolation Solution I

10760028-2 500 mL
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Plasmid DNA Isolation Solution II

10760029-1 250 mL
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10760029-2 500 mL
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EpiQuik Circulating Cell-Free DNA Isolation Kit

P-1064
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  • 50 Isolations
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Plasmid DNA Isolation Solution III

10760030-1 250 mL
EUR 28.07

Plasmid DNA Isolation Solution III

10760030-2 500 mL
EUR 43

Soil DNA Plus Isolation Mini Kit (100prep)

FAPSO-001-1 100 preps
EUR 241.2

FastPure Cell/Tissue DNA Isolation Mini Kit

DC102 100 rxn
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FastPure Cell/Tissue DNA Isolation Mini Kit

DC102-01 100 rxns
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Soil DNA Plus Isolation Mini Kit (50prep)

FAPSO-001 50 preps
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Methylamp Coupled DNA Isolation & Modification Kit

P-1002 40 Samples
EUR 474.12

QC Buffer for Plasmid DNA Isolation

10760036-1 250 mL
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QC Buffer for Plasmid DNA Isolation

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QF Buffer for Plasmid DNA Isolation

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QF Buffer for Plasmid DNA Isolation

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QF Buffer for Plasmid DNA Isolation

10760037-3 1 L
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One Tube Viral DNA Isolation Buffer

VT4785 50Preps
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One Tube Viral DNA Isolation Buffer

VT4786 100preps
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QBT Buffer for Plasmid DNA Isolation

10760035-1 250 mL
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QBT Buffer for Plasmid DNA Isolation

10760035-2 500 mL
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QBT Buffer for Plasmid DNA Isolation

10760035-3 1 L
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EpiMag High Throughput DNA Isolation Universal Kit

P-1022-96 96 Samples
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Hipro Circulating Cell-Free DNA (cfDNA) Isolation Kit

W2603-10 - Ask for price

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Hipro Circulating Cell-Free DNA (cfDNA) Isolation Kit

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Rna Isolation Kit

11828665001 EACH
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miRNA Isolation Kit

FAMIK--001 Rxns: 100
EUR 398.4
Description: Contents of the kit:
  • Rxns:100Lysis
  • Buffer-10 ml
  • Acid-Phenol-10 ml
  • RNA Column and Collection Tube-100 sets
  • Wash Buffer-10 ml
  • Release Buffer-0.5 ml

miRNA Isolation Kit

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miRNA Isolation Kit

FYG304-100P 100 Preps Ask for price

Exosome Isolation Kit

E601E001 20 tests
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MicroRNA Isolation Kit

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Endosome Isolation Kit

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Microsome Isolation Kit

K249-50 each
EUR 633.6

Protoplast Isolation Kit

30210002-1 20 Prep(s)
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Protoplast Isolation Kit

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Chloroplast Isolation Kit

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FastPure Blood/Cell/Tissue/Bacteria DNA Isolation Mini Kit

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FastPure Blood/Cell/Tissue/Bacteria DNA Isolation Mini Kit

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Mitochondria Isolation Kit

AR0156 1 kit
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Preadipocyte Isolation Kit

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Total RNA Isolation Kit

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Easy3-RNA Isolation Kit

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Easy3-RNA Isolation Kit

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Yeast Nuclei Isolation Kit

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Lipid Droplet Isolation Kit

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Description: Lipid droplets are lipid rich organelles found in the adipose tissue of eukaryotes. They function to regulate the hydrolysis and storage of neutral lipids and serve as storage for cholesterol and acyl-glycerols. Lipid droplets have also been associated with inflammatory responses, obesity, atherosclerosis, and cancer.

ExoPure? Isolation Kit   (Urine)

K1240-10 each
EUR 836.4

ExoPure? Isolation Kit   (Urine)

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EUR 483.6

Cartilage RNA Isolation Kit

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EUR 443

Apoptotic Cell Isolation Kit

55R-1349 30 units
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Description: Apoptotic Cell Isolation Kit for use in the research laboratory

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miRNA ISOLATION KIT-TISSUE

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miRNA ISOLATION KIT-TISSUE

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Total RNA Isolation Kit II

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EUR 369.6

Total RNA Isolation Kit II

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EVery EV RNA Isolation Kit

EVery100B-1 20 preps
EUR 412

EpiQuik Circulating Cell-Free DNA (ccfDNA) Isolation Easy Kit

P-1065
  • EUR 416.70
  • EUR 148.50
  • EUR 253.00
  • 50 Isolations
  • 25 Isolations
  • 50 Isolations

Exo-Urine EV Isolation Kit

EXOU100A-1 2 x 5 reactions
EUR 490

Cell Mitochondria Isolation Kit

abx090630-50100tests 50-100 tests
EUR 309.6

Cell Mitochondria Isolation Kit

ETF3601 100 assays
EUR 135

Rapid Blood RNA Isolation Kit

BT4182 10Preps
EUR 98.36

Rapid Blood RNA Isolation Kit

BT4183 50Preps
EUR 197.03

Rapid Blood RNA Isolation Kit

BT4184 250preps
EUR 608.1

Total RNA Isolation Kit (Plant)

P-9104 50 samples
EUR 416.7

Rapid Plant RNA Isolation Kit

PT4191 50Preps
EUR 95.63

Yeast Mitochondria Isolation Kit

K259-50 each
EUR 502.8

Total RNA Isolation Kit (Tissue)

P-9102 50 samples
EUR 416.7

EpiMag Viral RNA Isolation Kit

P-9108-050 50 samples
EUR 152.9

Tissue Mitochondria Isolation Kit

abx090631-50100assays 50-100 assays
EUR 309.6

Tissue Mitochondria Isolation Kit

ETF3606 100 assays
EUR 135

Lysosome Isolation Kit (20 tests)

P534 - Ask for price

Exosome Isolation kit (for urine)

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Exosome Isolation kit (for urine)

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Easy3-Plant RNA Isolation Kit

FYG502-100P 100 Preps Ask for price

Easy3-Plant RNA Isolation Kit

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CD19 Positive Cell Isolation Kit

78564-1 1 x 10^8 Cells
EUR 70
Description: The CD19 Positive Cell Isolation Kit is designed to magnetically separate CD19-expressing-cells from a complex immune cell population. This kit is optimized for the isolation of CD19 positive cells from normal human peripheral blood mononuclear cells (PBMCs). Cells are incubated with the antibody:bead complex and placed on a magnet for quick and easy separation. When placed on the magnet, CD19-positive cells will be immobilized along the side of the tube while undesired CD19-negative cells will remain in suspension for easy removal.

CD19 Positive Cell Isolation Kit

78564-2 1 x 10^9 Cells
EUR 630
Description: The CD19 Positive Cell Isolation Kit is designed to magnetically separate CD19-expressing-cells from a complex immune cell population. This kit is optimized for the isolation of CD19 positive cells from normal human peripheral blood mononuclear cells (PBMCs). Cells are incubated with the antibody:bead complex and placed on a magnet for quick and easy separation. When placed on the magnet, CD19-positive cells will be immobilized along the side of the tube while undesired CD19-negative cells will remain in suspension for easy removal.

Rapid Bacteria RNA Isolation Kit

BS8625 50Preps
EUR 95.63

ExoPure? Isolation Kit   (Cell Media)

K1237-10 each
EUR 796.8

ExoPure? Isolation Kit   (Cell Media)

K1237-2 each
EUR 470.4

ExoPure? Isolation Kit   (Bio Fluids)

K1241-10 each
EUR 836.4

ExoPure? Isolation Kit   (Bio Fluids)

K1241-2 each
EUR 483.6

Lipopolysaccharide (LPS) Isolation Kit

K438-10 each
EUR 333.6

ExoPure? Isolation Kit   (Serum, Plasma)

K1238-10 each
EUR 836.4

ExoPure? Isolation Kit   (Serum, Plasma)

K1238-2 each
EUR 470.4

Cell Surface Protein Isolation Kit

K295-10 each
EUR 601.2

GPC1 Capture Exosome Isolation Kit

E601E004 20 tests
EUR 2500

CD63 Capture Exosome Isolation Kit

E601E005 20 tests
EUR 2500

CD81 Capture Exosome Isolation Kit

E601E006 20 tests
EUR 2500

Breast cancer Exosome Isolation Kit

E601E002 20 tests
EUR 2500

EPCAM Capture Exosome Isolation Kit

E601E003 20 tests
EUR 2500

HSP70 Capture Exosome Isolation Kit

E601E007 20 tests
EUR 2500

EZExtract? Core Histone Isolation Kit

K234-100 each
EUR 314.4

10-min. Single Cell Isolation Kit

P713-20 - Ask for price

MagIso NK Cell Isolation Kit, Human

WHK-B023 20 tests, 200 tests Ask for price

DNA/RNA Mini Kit (isolation of both genomic DNA and total RNA from a single sample)

FADRK_050 1 kit
EUR 90

Circulating Nuleic Acid Isolation Kit

FACFK-050 50 preps
EUR 589.2

Circulating Nuleic Acid Isolation Kit

FACFK-100 100 preps
EUR 990

Detergent-Free Nuclei Isolation Kit

P524-2 - Ask for price

Detergent-Free Nuclei Isolation Kit

P524-20 - Ask for price

Detergent-Free Nuclei Isolation Kit

P524-80 - Ask for price

Mouse Brain Microvessel Isolation Kit

P750-4 - Ask for price

Circulating Nuleic Acid Isolation Kit

FACFK_050 1 kit
EUR 476

Broad Range Total RNA Isolation Kit

K1341050 1 kit
EUR 259

MagIso CD3 Cell Isolation Kit, Mouse

WHK-B009 20 tests, 200 tests Ask for price

Org Frontier? Chloroplast Isolation Kit

K468-10 each
EUR 666

The current hybrid hybrid is not easily dissociated into the biological buffers, which is thus suitable for use in “liquid biopsy”, requiring a long-term incubation process with biological samples. In addition, it is gradually degraded by the action of chelating agents; An effortless recovery of traffic markers has been performed. Then we modified the spheres of the CSID hydrogel with the anti-EPCAM antibody (“immunosphere CSID”) and the anti-CD63 antibody to isolate two promising circulation markers in the biopsy Liquid: CTC and CTXS. The capacities of the immunospheres for the isolation of markers and recovery have been confirmed by a fluorescence image, where the spheres isolate and effortlessly recover the target traffic markers.