Description: It is a semi quantitative competition ELISA kit to detect the Anti-SARS-CoV-2 Neutralization Antibody in human serum or plasma. The micro test plate provided in this kit is pre-coated with recombinant human ACE2. During the reaction, the SARS-CoV-2 neutralization antibody in the standard & sample diluent pretreated samples or controls competes with a fixed amount of human ACE2 on the solid phase supporter for sites on the Horseradish peroxidase (HRP) conjugated recombinant SARS-CoV-2 RBD fragment (HRP-RBD). After 37℃incubation, the unbound HRP-RBD as well as any HRP-RBD bound to non-neutralization antibody will be captured on the plate and eventually form the ACE2-RBD-HRP complex, while the circulating neutralization antibodies HRP-RBD complexes remain in the supernatant and are removed during washing. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm.

Description: SARS-CoV-2 Antigen ELISA Kit intended use is for quantitative detection of the recombinant SARS-COV-2 nucleoprotein antigen in human serum. The use of this kit for natural samples need to be validated by the end user due to the complexity of natural targets and unpredictable interference.

Description: SARS-CoV E Protein ( 34.7 kDa), recombinant protein from E. coli, 1 mg/mL.