Mesenchymal stem cell derived-exosomes: a modern approach in translational medicine

Mesenchymal stem cells (MSCs) have captured great attention in regenerative treatment for more than decades based on their differentiation capacity, strong immunomodulator properties, and their ability to be kululiped and manipulated. The recent investigation implies that the MSCS pleiotropic effect is not related to their differentiation capabilities, but is more mediated by the secretion of soluble paracrine factors. Exosom, nano extracellular vesicles, is one of these paracrine mediators.

Exosom functional cargo transfers such as MIRNA and MRNA molecules, peptides, protein, cytokines, and lipids from MSc to the recipient’s cell. Exocomic participates in communication events between cells and contributes to healing tissues and injured organs. Studies report that exosom alone is responsible for the therapeutic effect of MSC in various experimental models. Therefore, the exosom derived MSC can be manipulated and applied to establish a new cell-free therapeutic approach to the treatment of various diseases including the heart, kidney, liver, immune, and neurological disease, and healing of skin wounds. Compared to their donor cells, the exosom derived MSC offers more stable entities and reduced risk of safety regarding living cell administration, eg. Microvascular occlusion risk.

This review discusses the exosome insulation method found and utilized in clinical settings so far and present the latest information summary of the MSC exosomes in the treatment of translation. Exosom is an extracellular type of vesicle (EVS) issued by several types of mammal cells and involved in communication between cells. A number of studies have explored the potential diagnostic and exosomal therapeutic. The main challenge is the lack of standard and standard techniques for insulation and analysis of downstream nanovesicles. Conventional insulation method, such as ultracentrification, precipitation, filtration, chromatography, and an immune affinity-based approach, depends on certain physical properties or in surface biomarkers. However, one of the existing methods has limitations. Various parameters, such as efficacy, specificity, labor input, cost and scalability, and standardization options, must be considered for the appropriate approach.

The communication of extracellular vesicles between mesenchymal stroma cells and immune effect cells

Mesenchymal Stem / Stromal Cell (MSCS) is a multiphotic cell that is on the body’s stroma network and is able to promote network repair and weaken the inflammatory process through its immunomodulatory properties. Preclinical and clinical observations reveal that it is not only inter-cell communication that directly mediates MSC property; In fact, an important role is also played by the release of soluble and bioactive factors, such as cytokines, growth factors and extracellular vesicles (EVs). EVS is a membrane plated vesicel that contains various kinds of bioactive molecules, including lipids, proteins, and nucleic acids, such as RNA.

EVS let go of their content into target cells, thus affecting the fate of cells through intracellular process control. In addition, EV which is revealed MSC can mediate the effect of modulation towards different effector cells belonging to innate and adaptive immunity. In this review, we will discuss the literature data on EVS obtained by MSC, including the current standard method for their isolation and characterization, the mechanism that supports its immunoregulator properties, and their potential clinical applications as an alternative MSc-based therapy for inflammatory reactions, such as grafting disease Versus-hosted (GVHD).

 Mesenchymal stem cell derived-exosomes: a modern approach in translational medicine
Mesenchymal stem cell derived-exosomes: a modern approach in translational medicine

Large extracellular vesicles can be characterized by multiplex labeling using imaging flow cytometry

The extracellular vesicles (EVS) are heterogeneous (30 Nm-10 μm), content (lipid, RNA, DNA, protein) and potential function (s). Many isolation techniques systematically throw large EVS in the early stages of the small or exosomatic isolation protocols. We describe here a standardized method for isolating large eV from medulloblastoma cells and examine the expression of the EV marker and the diameter using the imaging flow cytometry. Our approach allows the characterization of each large EVS as an individual event, decorated with several fluorescent conjugal markers with the additional advantage of visualizing each event to ensure an application of robust gage strategies.

Methods: We describe the isolation of the step and characterization of a subset of large EVS from the UW228-2 medulloblastoma cell line evaluated by fluorescent light microscopy, transmission electron microscopy (TEM) and Tunable resistance detection (TRP). The viability of parental cells was evaluated by the Annexine V exposure by flow cytometry. Imagery flow cytometry (ImageReam Mark II) identified EVS by direct fluorescent membrane marking with orange cell mask (CMO) in conjunction with the EV markers. A rigorous guarantee algorithm based on the intensity of lateral diffusion and fluorescence has been applied and the expression of the EV markers CD63, CD9 and the lamp 1 evaluated.

Results: UW228-2 cells extend prolifically EVS upwards up to 6 μm. We show that the Imaging Flow Cytometer II allows a robust and reproducible analysis of the large EVS, including the evaluation of the diameter. We also demonstrate a correlation between the increase in the size of the EV and the co-expression of the filtered markers.

Rapid Bacteria RNA Isolation Kit

BS8625 50Preps
EUR 95.63

Easy3-Plant RNA Isolation Kit

FYG502-100P 100 Preps Ask for price

Easy3-Plant RNA Isolation Kit

FYG502-500P 500 Preps Ask for price

Total RNA Isolation Kit II

FATRS--001 100 preps
EUR 369.6

Total RNA Isolation Kit II

FATRS--050 50 preps
EUR 259.2

Rapid Plant RNA Isolation Kit

PT4191 50Preps
EUR 95.63

Total RNA Isolation Kit (Tissue)

P-9102 50 samples
EUR 416.7

Total RNA Isolation Kit (Plant)

P-9104 50 samples
EUR 416.7

EpiMag Viral RNA Isolation Kit

P-9108-050 50 samples
EUR 152.9

EVery EV RNA Isolation Kit

EVery100B-1 20 preps
EUR 412

Navy RNA Lysis Kit 50

NAVYE1-RNA 50pack
EUR 237.6
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Navy RNA Lysis Kit 250

NAVYE5-RNA 250pack
EUR 781.2
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Green RNA Lysis Kit 50

GREENE1-RNA 50pack
EUR 216
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Green RNA Lysis Kit 250

GREENE5-RNA 250pack
EUR 685.2
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Pink RNA Lysis Kit 50

PINKE1-RNA 50pack
EUR 213.6
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Pink RNA Lysis Kit 250

PINKE5-RNA 250pack
EUR 673.2
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RNA Lysis Kit 50

REDE1-RNA 50pack
EUR 230.4
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RNA Lysis Kit 250

REDE5-RNA 250pack
EUR 746.4
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RNA Lysis Kit 50

YELLOWE1-RNA 50pack
EUR 230.4
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RNA Lysis Kit 250

YELLOWE5-RNA 250pack
EUR 748.8
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

RNA-easy Isolation Reagent

R701-01 100 ml
EUR 201.6

RNA-easy Isolation Reagent

R701-02 200 ml
EUR 254.4

5E Navy RNA Lysis Kit 100

NAVY5E100-RNA 100pack
EUR 543.6
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

5E Navy RNA Lysis Kit 20

NAVY5E20-RNA 20pack
EUR 183.6
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Navy RINO RNA Lysis Kit 50

NAVYR1-RNA 50pack
EUR 242.4
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. RNase free.

Navy RINO RNA Lysis Kit 250

NAVYR5-RNA 250pack
EUR 799.2
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. RNase free.

5E GREEN RNA Lysis Kit 100

GREEN5E100-RNA 100pack
EUR 463.2
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 1000, 5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

5E GREEN RNA Lysis Kit 20

GREEN5E20-RNA 20pack
EUR 165.6
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Green RINO RNA Lysis Kit 50

GREENR1-RNA 50pack
EUR 220.8
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with beads. RNase free.

Green RINO RNA Lysis Kit 250

GREENR5-RNA 250pack
EUR 704.4
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with beads. RNase free.

5E PINK RNA Lysis Kit 100

PINK5E100-RNA 100pack
EUR 456
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

5E PINK RNA Lysis Kit 20

PINK5E20-RNA 20pack
EUR 163.2
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Pink RINO RNA Lysis Kit 50

PINKR1-RNA 50pack
EUR 217.2
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

Pink RINO RNA Lysis Kit 250

PINKR5-RNA 250pack
EUR 691.2
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

5E RED RNA Lysis Kit 100

RED5E100-RNA 100pack
EUR 514.8
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

5E RED RNA Lysis Kit 20

RED5E20-RNA 20pack
EUR 177.6
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RINO RNA Lysis Kit 50

REDR1-RNA 50pack
EUR 234
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

Red RINO RNA Lysis Kit 250

REDR5-RNA 250pack
EUR 765.6
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RINO RNA Lysis Kit 50

YELLOWR1-RNA 50pack
EUR 238.8
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RINO RNA Lysis Kit 250

YELLOWR5-RNA 250pack
EUR 787.2
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

Broad Range Total RNA Isolation Kit

K1341050 1 kit
EUR 319.2
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Soil RNA Isolation Mini Kit (50prep)

FASRK-001 50 preps
EUR 180

Soil RNA Isolation Mini Kit (100prep)

FASRK-100 100 preps
EUR 224.4

EpiQuik Total RNA Isolation Fast Kit

P-9105-050 50 samples
EUR 176

EpiQuik Magbeads Quick RNA Isolation Kit

P-9106-050 50 samples
EUR 163.9

EpiQuik Viral RNA Isolation Fast Kit

P-9107-050 50 samples
EUR 167.2

FastPure Cell/Tissue Total RNA Isolation Kit

RC101 50 rxn
EUR 274.8

PureXtract RNAsol RNA Isolation Solution, LS

R6103-120 2X60ml
EUR 343.2

FastPure Plant Total RNA Isolation Kit (Polysaccharides/Polyphenolics-Rich)

RC401 50 rxn
EUR 283.2

Mitochondria Isolation Kit

AR0156 1 kit
EUR 127.2

MicroRNA Isolation Kit

KS341025 1 kit
EUR 307.2
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

miRNA Isolation Kit

FYG304-100P 100 Preps Ask for price

miRNA Isolation Kit

FAMIK--001 Rxns: 100
EUR 398.4
Description: Contents of the kit:
  • Rxns:100Lysis
  • Buffer-10 ml
  • Acid-Phenol-10 ml
  • RNA Column and Collection Tube-100 sets
  • Wash Buffer-10 ml
  • Release Buffer-0.5 ml

miRNA Isolation Kit

FAMIK--002 Rxns: 50
EUR 267.6

Microsome Isolation Kit

K249-50 each
EUR 633.6

Preadipocyte Isolation Kit

K583-5 each
EUR 639.6

ExoQuick Exosome Isolation and RNA Purification kit (for Serum and Plasma)

EQ806A-1 20 preps
EUR 664.8

ExoQuick Exosome Isolation and RNA Purification kit (for Tissue Culture Media)

EQ806TC-1 10 preps
EUR 664.8

Apoptotic Cell Isolation Kit

55R-1349 30 units
EUR 874.8
Description: Apoptotic Cell Isolation Kit for use in the research laboratory

Mitochondrial DNA Isolation Kit

55R-1361 50 assays
EUR 990
Description: Mitochondrial DNA Isolation Kit for use in the research laboratory

Genomic DNA Isolation Kit

55R-1362 50 assays
EUR 462
Description: Genomic DNA Isolation Kit for use in the research laboratory

Cell Mitochondria Isolation Kit

abx090630-50100tests 50-100 tests
EUR 309.6

Tissue Mitochondria Isolation Kit

abx090631-50100assays 50-100 assays
EUR 309.6

Lipid Droplet Isolation Kit

MET-5011 50 preps
EUR 498
Description: Lipid droplets are lipid rich organelles found in the adipose tissue of eukaryotes. They function to regulate the hydrolysis and storage of neutral lipids and serve as storage for cholesterol and acyl-glycerols. Lipid droplets have also been associated with inflammatory responses, obesity, atherosclerosis, and cancer.

Mitochondrial DNA Isolation Kit

K2117-50 50 assays
EUR 718.8

Genomic DNA Isolation Kit

K2118-50 50 assays
EUR 385.2

Saliva DNA Isolation Kit

K5011050 1 kit
EUR 307.2

Urine DNA Isolation Kit

K5011150 1 kit
EUR 307.2

Stool DNA Isolation Kit

K5011250 1 kit
EUR 307.2

Serum DNA Isolation Kit

K5018100 1 kit
EUR 432

ExoPure? Isolation Kit   (Urine)

K1240-10 each
EUR 836.4

ExoPure? Isolation Kit   (Urine)

K1240-2 each
EUR 483.6

Apoptotic Cell Isolation Kit

K258-30 each
EUR 561.6

Yeast Mitochondria Isolation Kit

K259-50 each
EUR 502.8

Mitochondrial DNA Isolation Kit

K280-50 each
EUR 705.6

Genomic DNA Isolation Kit

K281-50 each
EUR 326.4

Yeast Nuclei Isolation Kit

K289-50 each
EUR 470.4

Lipopolysaccharide (LPS) Isolation Kit

K438-10 each
EUR 333.6

Frit Kit

FRIT-KIT 1each
EUR 148.8
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Column Packing Kit

PACK-KIT 1pack
EUR 1242
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

Exo-Urine EV Isolation Kit

EXOU100A-1 2 x 5 reactions
EUR 661.2

Easy3-Total NucleicAcid Isolation Kit

FYG504-100ML 100 ml Ask for price

Easy3-Total NucleicAcid Isolation Kit

FYG504-500ML 500 ml Ask for price

Circulating Nuleic Acid Isolation Kit

FACFK-050 50 preps
EUR 589.2

Circulating Nuleic Acid Isolation Kit

FACFK-100 100 preps
EUR 990

Genoirse Plant DNA Isolation Kit

GR101083 50
EUR 267.6

Apoptotic DNA Ladder Isolation Kit

K2045-50 50 assays
EUR 602.4

XCF Exosomal DNA Isolation Kit

XCF200A-1 20 rxn
EUR 463.2

FitAmp Urine DNA Isolation Kit 

P-1017-050 50 Samples
EUR 171.6

FitAmp Urine DNA Isolation Kit 

P-1017-100 100 Samples
EUR 292.6

ChromaFlash Chromatin Isolation & Shearing Kit 

P-2023-100 100 Preparations
EUR 189.2

FitAmp Gel DNA Isolation Kit

P-1007 100 Samples
EUR 324.48

FitAmp Urine DNA Isolation Kit 

P-1017
  • EUR 474.12
  • EUR 171.60
  • EUR 292.60
  • 100 Samples
  • 50 Samples
  • 100 Samples

ChromaFlash Chromatin Isolation & Shearing Kit 

P-2023
  • EUR 324.48
  • EUR 189.20
  • 100 Preperations
  • 100 Preparations

ExoPure? Isolation Kit   (Cell Media)

K1237-10 each
EUR 796.8

ExoPure? Isolation Kit   (Cell Media)

K1237-2 each
EUR 470.4

ExoPure? Isolation Kit   (Serum, Plasma)

K1238-10 each
EUR 836.4

ExoPure? Isolation Kit   (Serum, Plasma)

K1238-2 each
EUR 470.4

ExoPure? Isolation Kit   (Bio Fluids)

K1241-10 each
EUR 836.4

ExoPure? Isolation Kit   (Bio Fluids)

K1241-2 each
EUR 483.6

Apoptotic DNA Ladder Isolation Kit

K170-50 each
EUR 561.6

EZExtract? Core Histone Isolation Kit

K234-100 each
EUR 314.4

Cell Surface Protein Isolation Kit

K295-10 each
EUR 601.2

Bacterial Genomic DNA Isolation Kit

K309-100 each
EUR 451.2

OrgFrontier? Plasma Membrane Isolation Kit

K414-10 each
EUR 679.2

Org Frontier? Chloroplast Isolation Kit

K468-10 each
EUR 666

Whole Blood DNA Isolation Kit

K528-100 each
EUR 451.2

CD19 Positive Cell Isolation Kit

78564-1 1 x 10^8 Cells
EUR 70
Description: The CD19 Positive Cell Isolation Kit is designed to magnetically separate CD19-expressing-cells from a complex immune cell population. This kit is optimized for the isolation of CD19 positive cells from normal human peripheral blood mononuclear cells (PBMCs). Cells are incubated with the antibody:bead complex and placed on a magnet for quick and easy separation. When placed on the magnet, CD19-positive cells will be immobilized along the side of the tube while undesired CD19-negative cells will remain in suspension for easy removal.

CD19 Positive Cell Isolation Kit

78564-2 1 x 10^9 Cells
EUR 630
Description: The CD19 Positive Cell Isolation Kit is designed to magnetically separate CD19-expressing-cells from a complex immune cell population. This kit is optimized for the isolation of CD19 positive cells from normal human peripheral blood mononuclear cells (PBMCs). Cells are incubated with the antibody:bead complex and placed on a magnet for quick and easy separation. When placed on the magnet, CD19-positive cells will be immobilized along the side of the tube while undesired CD19-negative cells will remain in suspension for easy removal.

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 319.2

Human anti-cartilage-antibody(anti-cartilage-Ab) ELISA Kit

YLA0056HU-48T 48T
EUR 435

Human anti-cartilage-antibody(anti-cartilage-Ab) ELISA Kit

YLA0056HU-96T 96T
EUR 562.5

Orange Lysis Kit 50

ORANGEE1-RNA 50pack
EUR 198
Description: Bead lysis kits for extracting bacteria or generating single cell suspensions from tissue samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Orange Lysis Kit 250

ORANGEE5-RNA 250pack
EUR 644.4
Description: Bead lysis kits for extracting bacteria or generating single cell suspensions from tissue samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Mouse Cartilage PrimaCell: Normal Articular Cartilage

2-82096 1 Kit Ask for price

Rat Cartilage PrimaCell: Normal Articular Cartilage

2-82523 1 Kit Ask for price

Human Cartilage PrimaCell: Normal Articular Cartilage

2-96043 1 Kit Ask for price

PSEUDOMONAS ISOLATION AGAR

P16-114-10kg 10 kg
EUR 2058

PSEUDOMONAS ISOLATION AGAR

P16-114-2kg 2kg
EUR 494.4

PSEUDOMONAS ISOLATION AGAR

P16-114-500g 500 g
EUR 177.6

PSEUDOMONAS ISOLATION BROTH

P16-115-10kg 10 kg Ask for price

PSEUDOMONAS ISOLATION BROTH

P16-115-2Kg 2 Kg Ask for price

PSEUDOMONAS ISOLATION BROTH

P16-115-500g 500 g Ask for price

Orange RINO Lysis Kit 50

ORANGER1-RNA 50pack
EUR 202.8
Description: Bead lysis kits for extracting bacteria or generating single cell suspensions from tissue samples. Pack of 50, 1.5mL RINO tubes prefilled with stainless steel beads. RNase free.

Orange RINO Lysis Kit 250

ORANGER5-RNA 250pack
EUR 664.8
Description: Bead lysis kits for extracting bacteria or generating single cell suspensions from tissue samples. Pack of 250, 1.5mL RINO tubes prefilled with stainless steel beads. RNase free.

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit

CAS400A-KIT 1 kit (10 rxn)
EUR 1332

Pesticide Kit Containing All 10 Multi-Compound Mixes

SPXPR-KIT 1ML
EUR 1923.18

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit

CAS410A-KIT 1 kit (10 rxn)
EUR 798

Cas9-EGFP Protein and T7 gRNA SmartNuclease Synthesis Kit

CAS420A-KIT 1 kit (10 rxn)
EUR 828

RNA Kit

20-abx098089
  • EUR 126.00
  • EUR 410.40
  • 2 rxns
  • 50 rxns

CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV100PA-KIT 1 Kit
EUR 1358.4

CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV105PA-KIT 1 Kit
EUR 1358.4

MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV120PA-KIT 1 Kit
EUR 1358.4

MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV125PA-KIT 1 Kit
EUR 1358.4

Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS700A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS720A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS740A-KIT 10 rxn
EUR 1358.4

Mammalian Mitochondria Isolation Kit for Tissue

abx098857-50rxns 50 rxns
EUR 493.2

FastPure Bacteria DNA Isolation Mini Kit

DC103 100 rxn
EUR 212.4

FastPure Plant DNA Isolation Mini Kit

DC104 50 rxn
EUR 192

Conclusions: We have developed a labeling and gating strategy strictly capable of exploring the eV marker expression (CD63, CD9 and LAMP1) on individual EV in a largely heterogeneous population. Taken together, the data presented here strongly support the value of the exploration of large EVS in clinical samples for potential biomarkers, useful in the monitoring of diagnosis and disease surveillance.