Mesenchymal stem cell derived-exosomes: a modern approach in translational medicine

Mesenchymal stem cells (MSCs) have captured great attention in regenerative treatment for more than decades based on their differentiation capacity, strong immunomodulator properties, and their ability to be kululiped and manipulated. The recent investigation implies that the MSCS pleiotropic effect is not related to their differentiation capabilities, but is more mediated by the secretion of soluble paracrine factors. Exosom, nano extracellular vesicles, is one of these paracrine mediators.

Exosom functional cargo transfers such as MIRNA and MRNA molecules, peptides, protein, cytokines, and lipids from MSc to the recipient’s cell. Exocomic participates in communication events between cells and contributes to healing tissues and injured organs. Studies report that exosom alone is responsible for the therapeutic effect of MSC in various experimental models. Therefore, the exosom derived MSC can be manipulated and applied to establish a new cell-free therapeutic approach to the treatment of various diseases including the heart, kidney, liver, immune, and neurological disease, and healing of skin wounds. Compared to their donor cells, the exosom derived MSC offers more stable entities and reduced risk of safety regarding living cell administration, eg. Microvascular occlusion risk.

This review discusses the exosome insulation method found and utilized in clinical settings so far and present the latest information summary of the MSC exosomes in the treatment of translation. Exosom is an extracellular type of vesicle (EVS) issued by several types of mammal cells and involved in communication between cells. A number of studies have explored the potential diagnostic and exosomal therapeutic. The main challenge is the lack of standard and standard techniques for insulation and analysis of downstream nanovesicles. Conventional insulation method, such as ultracentrification, precipitation, filtration, chromatography, and an immune affinity-based approach, depends on certain physical properties or in surface biomarkers. However, one of the existing methods has limitations. Various parameters, such as efficacy, specificity, labor input, cost and scalability, and standardization options, must be considered for the appropriate approach.

The communication of extracellular vesicles between mesenchymal stroma cells and immune effect cells

Mesenchymal Stem / Stromal Cell (MSCS) is a multiphotic cell that is on the body’s stroma network and is able to promote network repair and weaken the inflammatory process through its immunomodulatory properties. Preclinical and clinical observations reveal that it is not only inter-cell communication that directly mediates MSC property; In fact, an important role is also played by the release of soluble and bioactive factors, such as cytokines, growth factors and extracellular vesicles (EVs). EVS is a membrane plated vesicel that contains various kinds of bioactive molecules, including lipids, proteins, and nucleic acids, such as RNA.

EVS let go of their content into target cells, thus affecting the fate of cells through intracellular process control. In addition, EV which is revealed MSC can mediate the effect of modulation towards different effector cells belonging to innate and adaptive immunity. In this review, we will discuss the literature data on EVS obtained by MSC, including the current standard method for their isolation and characterization, the mechanism that supports its immunoregulator properties, and their potential clinical applications as an alternative MSc-based therapy for inflammatory reactions, such as grafting disease Versus-hosted (GVHD).

 Mesenchymal stem cell derived-exosomes: a modern approach in translational medicine
Mesenchymal stem cell derived-exosomes: a modern approach in translational medicine

Large extracellular vesicles can be characterized by multiplex labeling using imaging flow cytometry

The extracellular vesicles (EVS) are heterogeneous (30 Nm-10 μm), content (lipid, RNA, DNA, protein) and potential function (s). Many isolation techniques systematically throw large EVS in the early stages of the small or exosomatic isolation protocols. We describe here a standardized method for isolating large eV from medulloblastoma cells and examine the expression of the EV marker and the diameter using the imaging flow cytometry. Our approach allows the characterization of each large EVS as an individual event, decorated with several fluorescent conjugal markers with the additional advantage of visualizing each event to ensure an application of robust gage strategies.

Methods: We describe the isolation of the step and characterization of a subset of large EVS from the UW228-2 medulloblastoma cell line evaluated by fluorescent light microscopy, transmission electron microscopy (TEM) and Tunable resistance detection (TRP). The viability of parental cells was evaluated by the Annexine V exposure by flow cytometry. Imagery flow cytometry (ImageReam Mark II) identified EVS by direct fluorescent membrane marking with orange cell mask (CMO) in conjunction with the EV markers. A rigorous guarantee algorithm based on the intensity of lateral diffusion and fluorescence has been applied and the expression of the EV markers CD63, CD9 and the lamp 1 evaluated.

Results: UW228-2 cells extend prolifically EVS upwards up to 6 μm. We show that the Imaging Flow Cytometer II allows a robust and reproducible analysis of the large EVS, including the evaluation of the diameter. We also demonstrate a correlation between the increase in the size of the EV and the co-expression of the filtered markers.

EVery EV RNA Isolation Kit

EVery100B-1 20 preps
EUR 412

Blood RNA Isolation(50) Kit     

TBS6002 50 samples
EUR 198

Viral DNA/RNA Isolation Kit

AGDK-0044 Kit Ask for price
Description: The Viral DNA/RNA Isolation Kit is intended for rapid co-extraction of viral DNA and RNA from a variety of biofluid samples, such as, plasma, serum, milk and swap samples. The proprietary microspherical paramagnetic beads used in the kit have a large binding surfaces and a high affinity towards nucleic acids. Going through sample lysis/binding, washing, and elution steps, the whole process can be completed under 35 minutes, and yields highly pure nucleic acids elute.  The recovered nucleic acids can be used in a wide range of applications, such as PCR, RT-PCR, Sanger Sequencing, NGS, and gene chips.

Rapid Blood RNA Isolation Kit

BT4182 10Preps
EUR 98.36

Rapid Blood RNA Isolation Kit

BT4183 50Preps
EUR 197.03

Rapid Blood RNA Isolation Kit

BT4184 250preps
EUR 608.1

Total RNA Isolation Kit (Plant)

PDC07-0100 100 rxns
EUR 209

Total RNA Isolation Kit (Plant)

P-9104 50 samples
EUR 416.7

Rapid Plant RNA Isolation Kit

PT4191 50Preps
EUR 95.63

Total RNA Isolation Kit(Tissue)

PDC08-0100 100 rxns
EUR 209

Total RNA Isolation Kit (Tissue)

P-9102 50 samples
EUR 416.7

EpiMag Viral RNA Isolation Kit

P-9108 50 samples
EUR 147

EpiMag Viral RNA Isolation Kit

P-9108-050 50 samples
EUR 152.9

Urine Exosome RNA Isolation Kit

47200 50 Preps
EUR 429

Easy3-Plant RNA Isolation Kit

FYG502-100P 100 Preps Ask for price

Easy3-Plant RNA Isolation Kit

FYG502-500P 500 Preps Ask for price

EXTRAClean Exosomal RNA Isolation Kit

72800 50 Preps
EUR 303

Total RNA Isolation Reagent Kit

abx460023-1096tests 10 × 96 tests Ask for price

Total RNA Isolation Reagent Kit

abx460023-596tests 5 × 96 tests Ask for price

Total RNA Isolation Reagent Kit

abx460023-96tests 96 tests
EUR 275

Total RNA Isolation Kit (Column Based)

MBS402031-100Reactions 100Reactions
EUR 400

Total RNA Isolation Kit (Column Based)

MBS402031-4Reactions 4Reactions
EUR 190

Easy3-RNA Isolation Kit

FYG501-100P 100 Preps Ask for price

Easy3-RNA Isolation Kit

FYG501-500P 500 Preps Ask for price

Phenol-Free Plant RNA Isolation Kit

10760056-1 50 Prep(s)
EUR 219.59

Column-Pure Fungi RNA Isolation Kit

D510 50 Preparations
EUR 150

RNApure Total RNA Rapid Isolation Kit

MBS355999-INQUIRE INQUIRE Ask for price

EpiQuik Magbeads Quick RNA Isolation Kit

P-9106 50 samples
EUR 154

EpiQuik Magbeads Quick RNA Isolation Kit

P-9106-050 50 samples
EUR 163.9

Soil RNA Isolation Mini Kit (100prep)

FASRK-100 100 preps
EUR 224.4

Soil RNA Isolation Mini Kit (50prep)

FASRK-001 50 preps
EUR 180

Total RNA Isolation Reagent

PDR03-0100 100 rxns
EUR 66

EpiQuik Total RNA Isolation Fast Kit

P-9105 50 samples
EUR 166

EpiQuik Total RNA Isolation Fast Kit

P-9105-050 50 samples
EUR 176

EpiQuik Viral RNA Isolation Fast Kit

P-9107 50 samples
EUR 161

EpiQuik Viral RNA Isolation Fast Kit

P-9107-050 50 samples
EUR 167.2

Total RNA Isolation Kit (Plant) (Column Based)

MBS402034-100Reactions 100Reactions
EUR 400

Total RNA Isolation Kit (Plant) (Column Based)

MBS402034-4Reactions 4Reactions
EUR 190

Plant Total RNA Isolation Reagent Kit

abx460024-1096tests 10 × 96 tests Ask for price

Plant Total RNA Isolation Reagent Kit

abx460024-596tests 5 × 96 tests Ask for price

Plant Total RNA Isolation Reagent Kit

abx460024-96tests 96 tests
EUR 362.5

Total RNA Isolation Kit(Tissue) (Column Based)

MBS402035-100Reactions 100Reactions
EUR 400

Total RNA Isolation Kit(Tissue) (Column Based)

MBS402035-4Reactions 4Reactions
EUR 190

FastPure Cell/Tissue Total RNA Isolation Kit

RC101 50 rxn
EUR 274.8

RNA-easy Isolation Reagent

R701-01 100 ml
EUR 201.6

RNA-easy Isolation Reagent

R701-02 200 ml
EUR 254.4

FastPure Universal Plant Total RNA Isolation Kit

RC411-00-10rxns 10 rxns
EUR 1.09

FastPure Universal Plant Total RNA Isolation Kit

RC411-01 50 rxns
EUR 100

FastPure Universal Plant Total RNA Isolation Kit

RC411-01-50rxns 50 rxns
EUR 129.71

Total RNA Isolation Kit II (Tri-reagent + RNA spin column)

FATRS__050 1 kit
EUR 62

Total RNA Isolation Kit II (Tri-reagent + RNA spin column)

FATRS__100 1 kit
EUR 112

FastPure Cell/Tissue Total RNA Isolation Kit V2

RC112-00-10rxns 10 rxns
EUR 1.09

FastPure Cell/Tissue Total RNA Isolation Kit V2

RC112-01 50 rxns
EUR 82.6

FastPure Cell/Tissue Total RNA Isolation Kit V2

RC112-01-50rxns 50 rxns
EUR 129.44

Plant Total RNA Isolation Reagent

PDR04-0100 100 rxns
EUR 110

Total RNA Isolation Kit (Blood/Cultured Cell/Fungus)

PDC04-0100 100 rxns
EUR 220

rSYNC™ RNA Isolation Kit - Individual Packed Columns

RS004 each
EUR 3

rSYNC™ RNA Isolation Kit - Individual Packed Columns

RS050 each
EUR 83.4

rSYNC™ RNA Isolation Kit - Individual Packed Columns

RS100 each
EUR 153

Type II Collagen (isolate from chicken cartilage)

COS-T2C1 1kg
EUR 174.4

miRNA Isolation Kit (Tri-reagent + RNA spin column)

FAMIK__001 1 kit
EUR 134.5

miRNA Isolation Kit (Tri-reagent + RNA spin column)

FAMIK__002 1 kit
EUR 73

Ultrapure Total RNA Rapid Isolation Kit (whole blood)

MBS356003-50Tests 50Tests
EUR 315

Total RNA Isolation Reagent (Reagent Based)

MBS402052-100mL 100mL
EUR 235

Total RNA Isolation Reagent (Reagent Based)

MBS402052-4Reactions 4Reactions
EUR 165

EASYspin RNA Isolation Kit (Ultramicro-volume, with DNase I)

MBS355959-50Tests 50Tests
EUR 455

EASYspin RNA Isolation Kit (Ultramicro-volume, with DNase I)

MBS355959-5x50Tests 5x50Tests
EUR 2050

PureXtract RNAsol RNA Isolation Solution, LS

R6103-120 2X60ml
EUR 343.2

Urine Exosome Purification and RNA Isolation Mini Kit

58400 50 Preps
EUR 843

Urine Exosome Purification and RNA Isolation Midi Kit

58700 25 Preps
EUR 797.4

Urine Exosome Purification and RNA Isolation Maxi Kit

58800 15 Preps
EUR 479.4

EASYspin RNA Isolation Kit (For yeast)(without Phenol & Chloroform)

MBS355954-50Tests 50Tests
EUR 395

EASYspin RNA Isolation Kit (For yeast)(without Phenol & Chloroform)

MBS355954-5x50Tests 5x50Tests
EUR 1755

miRNA Isolation Kit

FYG304-100P 100 Preps Ask for price

miRNA Isolation Kit

FAMIK--001 Rxns: 100
EUR 398.4
Description: Contents of the kit:
  • Rxns:100Lysis
  • Buffer-10 ml
  • Acid-Phenol-10 ml
  • RNA Column and Collection Tube-100 sets
  • Wash Buffer-10 ml
  • Release Buffer-0.5 ml

miRNA Isolation Kit

FAMIK--002 Rxns: 50
EUR 267.6

Exosome Isolation Kit

E601E001 20 tests
EUR 2500

Exosome Isolation Kit

EIK01 2T
EUR 800
Description: Chromatography Reagents

Exosome Isolation Kit

MBS8579443-20Tests 20Tests
EUR 2625

Exosome Isolation Kit

MBS8579443-5x20Tests 5x20Tests
EUR 12240

Endosome Isolation Kit

P528 - Ask for price

Microsome Isolation Kit

K249-50 each
EUR 633.6

Protoplast Isolation Kit

30210002-1 20 Prep(s)
EUR 272.26

Protoplast Isolation Kit

30210002-2 50 Prep(s)
EUR 564.44

Chloroplast Isolation Kit

30210000-1 20 Prep(s)
EUR 336

Mitochondria Isolation Kit

AR0156 1 kit
EUR 127.2

Mitochondria Isolation Kit

BC156-50T 50T
EUR 150

Preadipocyte Isolation Kit

K583-5 each
EUR 639.6

EASYspin RNA Isolation Kit (For Bacteria)(with DNA removation columns)

MBS355951-50Tests 50Tests
EUR 475

EASYspin RNA Isolation Kit (For Bacteria)(with DNA removation columns)

MBS355951-5x50Tests 5x50Tests
EUR 2135

rSYNC™ RNA Isolation Kit w/DNase Set - Individual Packed Columns

RSD004 each
EUR 5

rSYNC™ RNA Isolation Kit w/DNase Set - Individual Packed Columns

RSD050 each
EUR 103.4

rSYNC™ RNA Isolation Kit w/DNase Set - Individual Packed Columns

RSD100 each
EUR 193

rSYNC™ RNA Isolation Kit w/DNase Set - Individual Packed Columns

RSD300 each
EUR 518

Plant Total RNA Isolation Reagent (Reagent Based)

MBS402053-100mL 100mL
EUR 245

Plant Total RNA Isolation Reagent (Reagent Based)

MBS402053-4Reactions 4Reactions
EUR 165

Food DNA Isolation Kit

54500 50 Preps
EUR 101.4

Soil DNA Isolation Kit

26500 50 Preps
EUR 149.4

FastPure Plant Total RNA Isolation Kit (Polysaccharides&Polyphenolics –rich)

RC401-01 50 rxns
EUR 100

FastPure Plant Total RNA Isolation Kit (Polysaccharides&Polyphenolics –rich)

RC401-01-50rxns 50 rxns
EUR 129.44

Urine Exosome and Free-Circulating RNA Isolation Mini Kit

59200 50 Preps
EUR 941.4

Urine Exosome and Free-Circulating RNA Isolation Midi Kit

59300 25 Preps
EUR 843

Urine Exosome and Free-Circulating RNA Isolation Maxi Kit

59400 15 Preps
EUR 641.4

Phage DNA Isolation Kit

46800 50 Preps
EUR 161.4

Phage DNA Isolation Kit

46850 100 Preps
EUR 305.4

Stool DNA Isolation Kit

27600 50 Preps
EUR 171

Stool DNA Isolation Kit

27640 4 Preps
EUR 12

Urine DNA Isolation Kit

18100 50 Preps
EUR 285

EVtrap™ Isolation Kit

JOT-EV02-01-01 1 mL
EUR 202.9

EVlent™ Isolation Kit

JOT-EV02-05-01 1 mL
EUR 414.75

Urine DNA Isolation Kit

K5011150 1 kit
EUR 191.8

Stool DNA Isolation Kit

K5011250 1 kit
EUR 191.8

Serum DNA Isolation Kit

K5018100 1 kit
EUR 287.35

EASYspin RNA Isolation Kit (For Tissues & Cells)(Without Phenol & Chloroform)

MBS355950-50Tests 50Tests
EUR 435

EASYspin RNA Isolation Kit (For Tissues & Cells)(Without Phenol & Chloroform)

MBS355950-5x50Tests 5x50Tests
EUR 1935

PLANTpure Total RNA Rapid Isolation Kit (plants tissues or cells)

MBS356000-INQUIRE INQUIRE Ask for price

Plasma/Serum Exosome Purification and RNA Isolation Mini Kit

58300 50 Preps
EUR 627

Plasma/Serum Exosome Purification and RNA Isolation Midi Kit

58500 25 Preps
EUR 569.4

Plasma/Serum Exosome Purification and RNA Isolation Maxi Kit

58600 15 Preps
EUR 341.4

Microm DNA Isolation Kit

55300 50 Preps
EUR 131.4

Saliva DNA Isolation Kit

AG0226 Kit Ask for price
Description: Saliva DNA Isolation Kit provides a fast and simple spin column procedure for isolating high quality DNA from saliva samples collected and preserved using AcceGen's Saliva DNA Collection and Preservation Devices, as well as fresh saliva samples.Saliva DNA purified using AcceGen's kit is of the excellent quality, and is compatible with a number of downstream research applications including PCR, qPCR, Southern Blot analysis, sequencing and microarray analysis.

Saliva DNA Isolation Kit

K5011050 1 kit
EUR 191.8

Saliva DNA Isolation Kit

RU45400 50 Preps
EUR 101.4

Saliva DNA Isolation Kit

RU45410 4 Preps
EUR 12

FastPure Plant Total RNA Isolation Kit (Polysaccharides/Polyphenolics-Rich)

RC401 50 rxn
EUR 283.2

EASYspin RNA Isolation Kit (For Fibrous Tissues)(without Phenol & Chloroform)

MBS355952-50Tests 50Tests
EUR 485

EASYspin RNA Isolation Kit (For Fibrous Tissues)(without Phenol & Chloroform)

MBS355952-5x50Tests 5x50Tests
EUR 2190

Genomic DNA Isolation Kit

55R-1362 50 assays
EUR 273
Description: Genomic DNA Isolation Kit for use in the research laboratory

Biofilm DNA Isolation Kit

62300 50 Preps
EUR 243

Genomic DNA Isolation Kit

24700 50 Preps
EUR 99

Genomic DNA Isolation Kit

24750 100 Preps
EUR 185.4

Genomic DNA Isolation Kit

24770 250 Preps
EUR 441

Genomic DNA Isolation Kit

abx460025-1096tests 10 × 96 tests Ask for price

Genomic DNA Isolation Kit

abx460025-596tests 5 × 96 tests Ask for price

Genomic DNA Isolation Kit

abx460025-96tests 96 tests
EUR 437.5

Genomic DNA Isolation Kit

GWB-AXR187 50 assays Ask for price

Genomic DNA Isolation kit

MBK0069 50 TEST
EUR 366.24

Genomic DNA Isolation Kit

K2118-50 50 assays
EUR 601.6
Description: Tools Kit|DNA Isolation Kit#Kits|Tools Kit#Kits

Genomic DNA Isolation Kit

K281-50 each
EUR 326.4

Magnetic Pathogens Nucleic Acid (DNA/RNA)Isolation Kit, 50 reactions/pk

208601 8 pcs/cs
EUR 131.12
Description: Magnetic Pathogens Nucleic Acid (DNA/RNA)Isolation Kit, 50 reactions/pk

Conclusions: We have developed a labeling and gating strategy strictly capable of exploring the eV marker expression (CD63, CD9 and LAMP1) on individual EV in a largely heterogeneous population. Taken together, the data presented here strongly support the value of the exploration of large EVS in clinical samples for potential biomarkers, useful in the monitoring of diagnosis and disease surveillance.