Mesenchymal stem cell derived-exosomes: a modern approach in translational medicine

Mesenchymal stem cells (MSCs) have captured great attention in regenerative treatment for more than decades based on their differentiation capacity, strong immunomodulator properties, and their ability to be kululiped and manipulated. The recent investigation implies that the MSCS pleiotropic effect is not related to their differentiation capabilities, but is more mediated by the secretion of soluble paracrine factors. Exosom, nano extracellular vesicles, is one of these paracrine mediators.

Exosom functional cargo transfers such as MIRNA and MRNA molecules, peptides, protein, cytokines, and lipids from MSc to the recipient’s cell. Exocomic participates in communication events between cells and contributes to healing tissues and injured organs. Studies report that exosom alone is responsible for the therapeutic effect of MSC in various experimental models. Therefore, the exosom derived MSC can be manipulated and applied to establish a new cell-free therapeutic approach to the treatment of various diseases including the heart, kidney, liver, immune, and neurological disease, and healing of skin wounds. Compared to their donor cells, the exosom derived MSC offers more stable entities and reduced risk of safety regarding living cell administration, eg. Microvascular occlusion risk.

This review discusses the exosome insulation method found and utilized in clinical settings so far and present the latest information summary of the MSC exosomes in the treatment of translation. Exosom is an extracellular type of vesicle (EVS) issued by several types of mammal cells and involved in communication between cells. A number of studies have explored the potential diagnostic and exosomal therapeutic. The main challenge is the lack of standard and standard techniques for insulation and analysis of downstream nanovesicles. Conventional insulation method, such as ultracentrification, precipitation, filtration, chromatography, and an immune affinity-based approach, depends on certain physical properties or in surface biomarkers. However, one of the existing methods has limitations. Various parameters, such as efficacy, specificity, labor input, cost and scalability, and standardization options, must be considered for the appropriate approach.

The communication of extracellular vesicles between mesenchymal stroma cells and immune effect cells

Mesenchymal Stem / Stromal Cell (MSCS) is a multiphotic cell that is on the body’s stroma network and is able to promote network repair and weaken the inflammatory process through its immunomodulatory properties. Preclinical and clinical observations reveal that it is not only inter-cell communication that directly mediates MSC property; In fact, an important role is also played by the release of soluble and bioactive factors, such as cytokines, growth factors and extracellular vesicles (EVs). EVS is a membrane plated vesicel that contains various kinds of bioactive molecules, including lipids, proteins, and nucleic acids, such as RNA.

EVS let go of their content into target cells, thus affecting the fate of cells through intracellular process control. In addition, EV which is revealed MSC can mediate the effect of modulation towards different effector cells belonging to innate and adaptive immunity. In this review, we will discuss the literature data on EVS obtained by MSC, including the current standard method for their isolation and characterization, the mechanism that supports its immunoregulator properties, and their potential clinical applications as an alternative MSc-based therapy for inflammatory reactions, such as grafting disease Versus-hosted (GVHD).

 Mesenchymal stem cell derived-exosomes: a modern approach in translational medicine
Mesenchymal stem cell derived-exosomes: a modern approach in translational medicine

Large extracellular vesicles can be characterized by multiplex labeling using imaging flow cytometry

The extracellular vesicles (EVS) are heterogeneous (30 Nm-10 μm), content (lipid, RNA, DNA, protein) and potential function (s). Many isolation techniques systematically throw large EVS in the early stages of the small or exosomatic isolation protocols. We describe here a standardized method for isolating large eV from medulloblastoma cells and examine the expression of the EV marker and the diameter using the imaging flow cytometry. Our approach allows the characterization of each large EVS as an individual event, decorated with several fluorescent conjugal markers with the additional advantage of visualizing each event to ensure an application of robust gage strategies.

Methods: We describe the isolation of the step and characterization of a subset of large EVS from the UW228-2 medulloblastoma cell line evaluated by fluorescent light microscopy, transmission electron microscopy (TEM) and Tunable resistance detection (TRP). The viability of parental cells was evaluated by the Annexine V exposure by flow cytometry. Imagery flow cytometry (ImageReam Mark II) identified EVS by direct fluorescent membrane marking with orange cell mask (CMO) in conjunction with the EV markers. A rigorous guarantee algorithm based on the intensity of lateral diffusion and fluorescence has been applied and the expression of the EV markers CD63, CD9 and the lamp 1 evaluated.

Results: UW228-2 cells extend prolifically EVS upwards up to 6 μm. We show that the Imaging Flow Cytometer II allows a robust and reproducible analysis of the large EVS, including the evaluation of the diameter. We also demonstrate a correlation between the increase in the size of the EV and the co-expression of the filtered markers.

Blood RNA Isolation(50) Kit     

TBS6002 50 samples
EUR 198

Viral DNA/RNA Isolation Kit

AGDK-0044 Kit Ask for price
Description: The Viral DNA/RNA Isolation Kit is intended for rapid co-extraction of viral DNA and RNA from a variety of biofluid samples, such as, plasma, serum, milk and swap samples. The proprietary microspherical paramagnetic beads used in the kit have a large binding surfaces and a high affinity towards nucleic acids. Going through sample lysis/binding, washing, and elution steps, the whole process can be completed under 35 minutes, and yields highly pure nucleic acids elute.  The recovered nucleic acids can be used in a wide range of applications, such as PCR, RT-PCR, Sanger Sequencing, NGS, and gene chips.

Rapid Blood RNA Isolation Kit

BT4182 10Preps
EUR 98.36

Rapid Blood RNA Isolation Kit

BT4183 50Preps
EUR 197.03

Rapid Blood RNA Isolation Kit

BT4184 250preps
EUR 608.1

Rapid Plant RNA Isolation Kit

PT4191 50Preps
EUR 95.63

Total RNA Isolation Kit (Plant)

P-9104 50 samples
EUR 416.7

Total RNA Isolation Kit (Plant)

PDC07-0100 100 rxns
EUR 209

Total RNA Isolation Kit (Tissue)

P-9102 50 samples
EUR 416.7

EpiMag Viral RNA Isolation Kit

P-9108-050 50 samples
EUR 152.9

EpiMag Viral RNA Isolation Kit

P-9108 50 samples
EUR 144

Total RNA Isolation Kit(Tissue)

PDC08-0100 100 rxns
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Easy3-Plant RNA Isolation Kit

FYG502-100P 100 Preps Ask for price

Easy3-Plant RNA Isolation Kit

FYG502-500P 500 Preps Ask for price

Rapid Bacteria RNA Isolation Kit

BS8625 50Preps
EUR 95.63

Total RNA Isolation Reagent Kit

abx460023-1096tests 10 × 96 tests Ask for price

Total RNA Isolation Reagent Kit

abx460023-596tests 5 × 96 tests Ask for price

Total RNA Isolation Reagent Kit

abx460023-96tests 96 tests
EUR 275

Broad Range Total RNA Isolation Kit

K1341050 1 kit
EUR 298

Phenol-Free Plant RNA Isolation Kit

10760056-1 50 Prep(s)
EUR 170.53

Column-Pure Fungi RNA Isolation Kit

D510 50 Preparations
EUR 150

Easy3-RNA Isolation Kit

FYG501-100P 100 Preps Ask for price

Easy3-RNA Isolation Kit

FYG501-500P 500 Preps Ask for price

EpiQuik Magbeads Quick RNA Isolation Kit

P-9106-050 50 samples
EUR 163.9

EpiQuik Magbeads Quick RNA Isolation Kit

P-9106 50 samples
EUR 151

Soil RNA Isolation Mini Kit (100prep)

FASRK-100 100 preps
EUR 224.4

Soil RNA Isolation Mini Kit (50prep)

FASRK-001 50 preps
EUR 180

EpiQuik Total RNA Isolation Fast Kit

P-9105-050 50 samples
EUR 176

EpiQuik Viral RNA Isolation Fast Kit

P-9107-050 50 samples
EUR 167.2

EpiQuik Total RNA Isolation Fast Kit

P-9105 50 samples
EUR 163

EpiQuik Viral RNA Isolation Fast Kit

P-9107 50 samples
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Total RNA Isolation Reagent

PDR03-0100 100 rxns
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Plant Total RNA Isolation Reagent Kit

abx460024-1096tests 10 × 96 tests Ask for price

Plant Total RNA Isolation Reagent Kit

abx460024-596tests 5 × 96 tests Ask for price

Plant Total RNA Isolation Reagent Kit

abx460024-96tests 96 tests
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FastPure Cell/Tissue Total RNA Isolation Kit

RC101 50 rxn
EUR 274.8

RNA-easy Isolation Reagent

R701-01 100 ml
EUR 201.6

RNA-easy Isolation Reagent

R701-02 200 ml
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FastPure Universal Plant Total RNA Isolation Kit

RC411-01 50 rxns
EUR 100

Total RNA Isolation Kit II (Tri-reagent + RNA spin column)

FATRS__050 1 kit
EUR 62

Total RNA Isolation Kit II (Tri-reagent + RNA spin column)

FATRS__100 1 kit
EUR 112

FastPure Cell/Tissue Total RNA Isolation Kit V2

RC112-01 50 rxns
EUR 82.6

Plant Total RNA Isolation Reagent

PDR04-0100 100 rxns
EUR 110

Total RNA Isolation Kit (Blood/Cultured Cell/Fungus)

PDC04-0100 100 rxns
EUR 220

rSYNC™ RNA Isolation Kit - Individual Packed Columns

RS004 each
EUR 3

rSYNC™ RNA Isolation Kit - Individual Packed Columns

RS050 each
EUR 83.4

rSYNC™ RNA Isolation Kit - Individual Packed Columns

RS100 each
EUR 153

rSYNC™ RNA Isolation Kit - Individual Packed Columns

RS300 each
EUR 408

miRNA Isolation Kit (Tri-reagent + RNA spin column)

FAMIK__001 1 kit
EUR 134.5

miRNA Isolation Kit (Tri-reagent + RNA spin column)

FAMIK__002 1 kit
EUR 73

PureXtract RNAsol RNA Isolation Solution, LS

R6103-120 2X60ml
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miRNA Isolation Kit

FYG304-100P 100 Preps Ask for price

miRNA Isolation Kit

FAMIK--001 Rxns: 100
EUR 398.4
Description: Contents of the kit:
  • Rxns:100Lysis
  • Buffer-10 ml
  • Acid-Phenol-10 ml
  • RNA Column and Collection Tube-100 sets
  • Wash Buffer-10 ml
  • Release Buffer-0.5 ml

miRNA Isolation Kit

FAMIK--002 Rxns: 50
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Exosome Isolation Kit

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rSYNC™ RNA Isolation Kit w/DNase Set - Individual Packed Columns

RSD004 each
EUR 5

rSYNC™ RNA Isolation Kit w/DNase Set - Individual Packed Columns

RSD050 each
EUR 103.4

rSYNC™ RNA Isolation Kit w/DNase Set - Individual Packed Columns

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rSYNC™ RNA Isolation Kit w/DNase Set - Individual Packed Columns

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MicroRNA Isolation Kit

KS341025 1 kit
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Endosome Isolation Kit

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Microsome Isolation Kit

K249-50 each
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FastPure Plant Total RNA Isolation Kit (Polysaccharides&Polyphenolics –rich)

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Protoplast Isolation Kit

30210002-1 20 Prep(s)
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Protoplast Isolation Kit

30210002-2 50 Prep(s)
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FastPure Plant Total RNA Isolation Kit (Polysaccharides/Polyphenolics-Rich)

RC401 50 rxn
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Chloroplast Isolation Kit

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Magnetic Pathogens Nucleic Acid (DNA/RNA)Isolation Kit, 50 reactions/pk

208601 8 pcs/cs
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Description: Magnetic Pathogens Nucleic Acid (DNA/RNA)Isolation Kit, 50 reactions/pk

CELLDATA RNAstorm™ Fresh Cell and Tissue RNA Isolation Kit, 50 preps

CD504 1
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Description: DNAse I from bovine pancreas (cow; Bos taurus)

CELLDATA RNAstorm™ Fresh Cell and Tissue RNA Isolation Kit, 50 preps

CD504-1 KT
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Mitochondria Isolation Kit

AR0156 1 kit
EUR 127.2

Preadipocyte Isolation Kit

K583-5 each
EUR 639.6

Urine DNA Isolation Kit

K5011150 1 kit
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Stool DNA Isolation Kit

K5011250 1 kit
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Serum DNA Isolation Kit

K5018100 1 kit
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Rat Cartilage Total RNA

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Saliva DNA Isolation Kit

AG0226 Kit Ask for price
Description: Saliva DNA Isolation Kit provides a fast and simple spin column procedure for isolating high quality DNA from saliva samples collected and preserved using AcceGen's Saliva DNA Collection and Preservation Devices, as well as fresh saliva samples.Saliva DNA purified using AcceGen's kit is of the excellent quality, and is compatible with a number of downstream research applications including PCR, qPCR, Southern Blot analysis, sequencing and microarray analysis.

Saliva DNA Isolation Kit

K5011050 1 kit
EUR 261

Genomic DNA Isolation Kit

55R-1362 50 assays
EUR 273
Description: Genomic DNA Isolation Kit for use in the research laboratory

Genomic DNA Isolation Kit

GWB-AXR187 50 assays Ask for price

Genomic DNA Isolation Kit

K2118-50 50 assays
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Description: Isolates highly pure Genomic DNA within 90 min.

Genomic DNA Isolation Kit

K281-50 each
EUR 326.4

Genomic DNA Isolation Kit

abx460025-1096tests 10 × 96 tests Ask for price

Genomic DNA Isolation Kit

abx460025-596tests 5 × 96 tests Ask for price

Genomic DNA Isolation Kit

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EUR 437.5

Yeast Nuclei Isolation Kit

K289-50 each
EUR 470.4

ExoPure? Isolation Kit   (Urine)

K1240-10 each
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ExoPure? Isolation Kit   (Urine)

K1240-2 each
EUR 483.6

Lipid Droplet Isolation Kit

MET-5011 50 preps
EUR 345

Apoptotic Cell Isolation Kit

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Description: Apoptotic Cell Isolation Kit for use in the research laboratory

miRNA ISOLATION KIT-TISSUE

IB47370 4 PREP KIT
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miRNA ISOLATION KIT-TISSUE

IB47371 100 PREP KIT
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Apoptotic Cell Isolation Kit

GWB-AXR172 30isolations Ask for price

Apoptotic Cell Isolation Kit

K258-30 each
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Exosome Isolation kit (for serum & plasma -Exosome-RNA-NGS)

P101NGS - Ask for price

Rat WS Cartilage Total RNA

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Cell Mitochondria Isolation Kit

abx090630-50100tests 50-100 tests
EUR 309.6

Mitochondrial DNA Isolation Kit

55R-1361 50 assays
EUR 660
Description: Mitochondrial DNA Isolation Kit for use in the research laboratory

Mitochondrial DNA Isolation Kit

GWB-AXR186 50 assays Ask for price

Cell Mitochondria Isolation Kit

ETF3601 100 assays
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Mitochondrial DNA Isolation Kit

K2117-1 1 kit
EUR 686
Description: Isolates mt. DNA, no genomic DNA contamination

Mitochondrial DNA Isolation Kit

K2117-50 50 assays
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Mitochondrial DNA Isolation Kit

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Cell Mitochondria Isolation Kit

abx090630-100l 100 µl
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Cell Mitochondria Isolation Kit

abx090630-1ml 1 ml Ask for price

Cell Mitochondria Isolation Kit

abx090630-200l 200 µl Ask for price

Exo-Urine EV Isolation Kit

EXOU100A-1 2 x 5 reactions
EUR 490

FitAmp Gel DNA Isolation Kit

P-1007 100 Samples
EUR 324.48

Yeast Mitochondria Isolation Kit

K259-50 each
EUR 502.8

Plant/Fungi DNA Isolation Kit

10760114-1 50 Prep(s)
EUR 350.39

Whole Blood DNA Isolation Kit

K528-100 each
EUR 451.2

Tissue Mitochondria Isolation Kit

abx090631-50100assays 50-100 assays
EUR 309.6

Tissue Mitochondria Isolation Kit

ETF3606 100 assays
EUR 135

Tissue Mitochondria Isolation Kit

abx090631-100l 100 µl
EUR 187.5

Tissue Mitochondria Isolation Kit

abx090631-1ml 1 ml Ask for price

Tissue Mitochondria Isolation Kit

abx090631-200l 200 µl Ask for price

FitAmp Urine DNA Isolation Kit 

P-1017
  • Ask for price
  • Ask for price
  • Ask for price
  • 100 Samples
  • 50 Samples
  • 100 Samples

XCF Exosomal DNA Isolation Kit

XCF200A-1 20 rxn
EUR 328

Mouse CD1 Cartilage Total RNA

MR-109 0.025mg
EUR 214

Mouse C57 Cartilage Total RNA

MR-109-C57 0.025mg
EUR 267

FastPure FFPE DNA Isolation Kit

DC105-01 50 rxns
EUR 75.2

Exosome Isolation kit (for urine)

P120L 40 rxn
EUR 769

Exosome Isolation kit (for urine)

P120S 2 rxn
EUR 69

Lysosome Isolation Kit (20 tests)

P534 - Ask for price

Genomic DNA Isolation Kit (Plant)

PDC10-0100 100 rxns
EUR 132

CD19 Positive Cell Isolation Kit

78564-1 1 x 10^8 Cells
EUR 70
Description: The CD19 Positive Cell Isolation Kit is designed to magnetically separate CD19-expressing-cells from a complex immune cell population. This kit is optimized for the isolation of CD19 positive cells from normal human peripheral blood mononuclear cells (PBMCs). Cells are incubated with the antibody:bead complex and placed on a magnet for quick and easy separation. When placed on the magnet, CD19-positive cells will be immobilized along the side of the tube while undesired CD19-negative cells will remain in suspension for easy removal.

CD19 Positive Cell Isolation Kit

78564-2 1 x 10^9 Cells
EUR 630
Description: The CD19 Positive Cell Isolation Kit is designed to magnetically separate CD19-expressing-cells from a complex immune cell population. This kit is optimized for the isolation of CD19 positive cells from normal human peripheral blood mononuclear cells (PBMCs). Cells are incubated with the antibody:bead complex and placed on a magnet for quick and easy separation. When placed on the magnet, CD19-positive cells will be immobilized along the side of the tube while undesired CD19-negative cells will remain in suspension for easy removal.

Genoirse Plant DNA Isolation Kit

GR101083 50
EUR 223

ExoPure? Isolation Kit   (Cell Media)

K1237-10 each
EUR 796.8

ExoPure? Isolation Kit   (Cell Media)

K1237-2 each
EUR 470.4

ExoPure? Isolation Kit   (Bio Fluids)

K1241-10 each
EUR 836.4

ExoPure? Isolation Kit   (Bio Fluids)

K1241-2 each
EUR 483.6

Virus Nucleic Acid Isolation Kit

PDC03-0100 100 rxns
EUR 198

Genomic DNA Isolation Kit (Tissue)

PDC11-0100 100 rxns
EUR 132

Lipopolysaccharide (LPS) Isolation Kit

K438-10 each
EUR 333.6

DNA/RNA Mini Kit (isolation of both genomic DNA and total RNA from a single sample)

FADRK_050 1 kit
EUR 90

Mouse Balbc Cartilage Total RNA

MR-109-BLC 0.025mg
EUR 218

GPC1 Capture Exosome Isolation Kit

E601E004 20 tests
EUR 2500

CD63 Capture Exosome Isolation Kit

E601E005 20 tests
EUR 2500

CD81 Capture Exosome Isolation Kit

E601E006 20 tests
EUR 2500

Conclusions: We have developed a labeling and gating strategy strictly capable of exploring the eV marker expression (CD63, CD9 and LAMP1) on individual EV in a largely heterogeneous population. Taken together, the data presented here strongly support the value of the exploration of large EVS in clinical samples for potential biomarkers, useful in the monitoring of diagnosis and disease surveillance.