Mesenchymal stem cells (MSCs) have captured great attention in regenerative treatment for more than decades based on their differentiation capacity, strong immunomodulator properties, and their ability to be kululiped and manipulated. The recent investigation implies that the MSCS pleiotropic effect is not related to their differentiation capabilities, but is more mediated by the secretion of soluble paracrine factors. Exosom, nano extracellular vesicles, is one of these paracrine mediators.

Exosom functional cargo transfers such as MIRNA and MRNA molecules, peptides, protein, cytokines, and lipids from MSc to the recipient’s cell. Exocomic participates in communication events between cells and contributes to healing tissues and injured organs. Studies report that exosom alone is responsible for the therapeutic effect of MSC in various experimental models. Therefore, the exosom derived MSC can be manipulated and applied to establish a new cell-free therapeutic approach to the treatment of various diseases including the heart, kidney, liver, immune, and neurological disease, and healing of skin wounds. Compared to their donor cells, the exosom derived MSC offers more stable entities and reduced risk of safety regarding living cell administration, eg. Microvascular occlusion risk.

This review discusses the exosome insulation method found and utilized in clinical settings so far and present the latest information summary of the MSC exosomes in the treatment of translation. Exosom is an extracellular type of vesicle (EVS) issued by several types of mammal cells and involved in communication between cells. A number of studies have explored the potential diagnostic and exosomal therapeutic. The main challenge is the lack of standard and standard techniques for insulation and analysis of downstream nanovesicles. Conventional insulation method, such as ultracentrification, precipitation, filtration, chromatography, and an immune affinity-based approach, depends on certain physical properties or in surface biomarkers. However, one of the existing methods has limitations. Various parameters, such as efficacy, specificity, labor input, cost and scalability, and standardization options, must be considered for the appropriate approach.

The communication of extracellular vesicles between mesenchymal stroma cells and immune effect cells

Mesenchymal Stem / Stromal Cell (MSCS) is a multiphotic cell that is on the body’s stroma network and is able to promote network repair and weaken the inflammatory process through its immunomodulatory properties. Preclinical and clinical observations reveal that it is not only inter-cell communication that directly mediates MSC property; In fact, an important role is also played by the release of soluble and bioactive factors, such as cytokines, growth factors and extracellular vesicles (EVs). EVS is a membrane plated vesicel that contains various kinds of bioactive molecules, including lipids, proteins, and nucleic acids, such as RNA.

EVS let go of their content into target cells, thus affecting the fate of cells through intracellular process control. In addition, EV which is revealed MSC can mediate the effect of modulation towards different effector cells belonging to innate and adaptive immunity. In this review, we will discuss the literature data on EVS obtained by MSC, including the current standard method for their isolation and characterization, the mechanism that supports its immunoregulator properties, and their potential clinical applications as an alternative MSc-based therapy for inflammatory reactions, such as grafting disease Versus-hosted (GVHD).

Large extracellular vesicles can be characterized by multiplex labeling using imaging flow cytometry

The extracellular vesicles (EVS) are heterogeneous (30 Nm-10 μm), content (lipid, RNA, DNA, protein) and potential function (s). Many isolation techniques systematically throw large EVS in the early stages of the small or exosomatic isolation protocols. We describe here a standardized method for isolating large eV from medulloblastoma cells and examine the expression of the EV marker and the diameter using the imaging flow cytometry. Our approach allows the characterization of each large EVS as an individual event, decorated with several fluorescent conjugal markers with the additional advantage of visualizing each event to ensure an application of robust gage strategies.

Methods: We describe the isolation of the step and characterization of a subset of large EVS from the UW228-2 medulloblastoma cell line evaluated by fluorescent light microscopy, transmission electron microscopy (TEM) and Tunable resistance detection (TRP). The viability of parental cells was evaluated by the Annexine V exposure by flow cytometry. Imagery flow cytometry (ImageReam Mark II) identified EVS by direct fluorescent membrane marking with orange cell mask (CMO) in conjunction with the EV markers. A rigorous guarantee algorithm based on the intensity of lateral diffusion and fluorescence has been applied and the expression of the EV markers CD63, CD9 and the lamp 1 evaluated.

Results: UW228-2 cells extend prolifically EVS upwards up to 6 μm. We show that the Imaging Flow Cytometer II allows a robust and reproducible analysis of the large EVS, including the evaluation of the diameter. We also demonstrate a correlation between the increase in the size of the EV and the co-expression of the filtered markers.

Rapid Bacteria RNA Isolation Kit
BS8625	Bio Basic	50Preps	EUR 95.63

Easy3-Plant RNA Isolation Kit
FYG502-100P	Yeastern Biotech	100 Preps	Ask for price

Easy3-Plant RNA Isolation Kit
FYG502-500P	Yeastern Biotech	500 Preps	Ask for price

Total RNA Isolation Kit II
FATRS--001	Favorgen	100 preps	EUR 369.6

Total RNA Isolation Kit II
FATRS--050	Favorgen	50 preps	EUR 259.2

Rapid Plant RNA Isolation Kit
PT4191	Bio Basic	50Preps	EUR 95.63

Total RNA Isolation Kit (Tissue)
P-9102	EpiGentek	50 samples	EUR 416.7

Total RNA Isolation Kit (Plant)
P-9104	EpiGentek	50 samples	EUR 416.7

EpiMag Viral RNA Isolation Kit
P-9108-050	EpiGentek	50 samples	EUR 152.9

EVery EV RNA Isolation Kit
EVery100B-1	SBI	20 preps	EUR 412

Navy RNA Lysis Kit 50
NAVYE1-RNA	Next Advance	50pack	EUR 237.6
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Navy RNA Lysis Kit 250
NAVYE5-RNA	Next Advance	250pack	EUR 781.2
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Green RNA Lysis Kit 50
GREENE1-RNA	Next Advance	50pack	EUR 216
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Green RNA Lysis Kit 250
GREENE5-RNA	Next Advance	250pack	EUR 685.2
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Pink RNA Lysis Kit 50
PINKE1-RNA	Next Advance	50pack	EUR 213.6
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Pink RNA Lysis Kit 250
PINKE5-RNA	Next Advance	250pack	EUR 673.2
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RNA Lysis Kit 50
REDE1-RNA	Next Advance	50pack	EUR 230.4
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RNA Lysis Kit 250
REDE5-RNA	Next Advance	250pack	EUR 746.4
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RNA Lysis Kit 50
YELLOWE1-RNA	Next Advance	50pack	EUR 230.4
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RNA Lysis Kit 250
YELLOWE5-RNA	Next Advance	250pack	EUR 748.8
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

RNA-easy Isolation Reagent
R701-01	Vazyme	100 ml	EUR 201.6

RNA-easy Isolation Reagent
R701-02	Vazyme	200 ml	EUR 254.4

5E Navy RNA Lysis Kit 100
NAVY5E100-RNA	Next Advance	100pack	EUR 543.6
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

5E Navy RNA Lysis Kit 20
NAVY5E20-RNA	Next Advance	20pack	EUR 183.6
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Navy RINO RNA Lysis Kit 50
NAVYR1-RNA	Next Advance	50pack	EUR 242.4
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. RNase free.

Navy RINO RNA Lysis Kit 250
NAVYR5-RNA	Next Advance	250pack	EUR 799.2
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. RNase free.

5E GREEN RNA Lysis Kit 100
GREEN5E100-RNA	Next Advance	100pack	EUR 463.2
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 1000, 5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

5E GREEN RNA Lysis Kit 20
GREEN5E20-RNA	Next Advance	20pack	EUR 165.6
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Green RINO RNA Lysis Kit 50
GREENR1-RNA	Next Advance	50pack	EUR 220.8
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with beads. RNase free.

Green RINO RNA Lysis Kit 250
GREENR5-RNA	Next Advance	250pack	EUR 704.4
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with beads. RNase free.

5E PINK RNA Lysis Kit 100
PINK5E100-RNA	Next Advance	100pack	EUR 456
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

5E PINK RNA Lysis Kit 20
PINK5E20-RNA	Next Advance	20pack	EUR 163.2
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Pink RINO RNA Lysis Kit 50
PINKR1-RNA	Next Advance	50pack	EUR 217.2
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

Pink RINO RNA Lysis Kit 250
PINKR5-RNA	Next Advance	250pack	EUR 691.2
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

5E RED RNA Lysis Kit 100
RED5E100-RNA	Next Advance	100pack	EUR 514.8
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

5E RED RNA Lysis Kit 20
RED5E20-RNA	Next Advance	20pack	EUR 177.6
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RINO RNA Lysis Kit 50
REDR1-RNA	Next Advance	50pack	EUR 234
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

Red RINO RNA Lysis Kit 250
REDR5-RNA	Next Advance	250pack	EUR 765.6
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RINO RNA Lysis Kit 50
YELLOWR1-RNA	Next Advance	50pack	EUR 238.8
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RINO RNA Lysis Kit 250
YELLOWR5-RNA	Next Advance	250pack	EUR 787.2
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

Broad Range Total RNA Isolation Kit
K1341050	Biochain	1 kit	EUR 319.2
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Soil RNA Isolation Mini Kit (50prep)
FASRK-001	Favorgen	50 preps	EUR 180

Soil RNA Isolation Mini Kit (100prep)
FASRK-100	Favorgen	100 preps	EUR 224.4

EpiQuik Total RNA Isolation Fast Kit
P-9105-050	EpiGentek	50 samples	EUR 176

EpiQuik Magbeads Quick RNA Isolation Kit
P-9106-050	EpiGentek	50 samples	EUR 163.9

EpiQuik Viral RNA Isolation Fast Kit
P-9107-050	EpiGentek	50 samples	EUR 167.2

FastPure Cell/Tissue Total RNA Isolation Kit
RC101	Vazyme	50 rxn	EUR 274.8

PureXtract RNAsol RNA Isolation Solution, LS
R6103-120	GenDepot	2X60ml	EUR 343.2

FastPure Plant Total RNA Isolation Kit (Polysaccharides/Polyphenolics-Rich)
RC401	Vazyme	50 rxn	EUR 283.2

Mitochondria Isolation Kit
AR0156	BosterBio	1 kit	EUR 127.2

MicroRNA Isolation Kit
KS341025	Biochain	1 kit	EUR 307.2
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

miRNA Isolation Kit
FYG304-100P	Yeastern Biotech	100 Preps	Ask for price

miRNA Isolation Kit
FAMIK--001	Favorgen	Rxns: 100	EUR 398.4
Description: Contents of the kit: Rxns:100Lysis Buffer-10 ml Acid-Phenol-10 ml RNA Column and Collection Tube-100 sets Wash Buffer-10 ml Release Buffer-0.5 ml

miRNA Isolation Kit
FAMIK--002	Favorgen	Rxns: 50	EUR 267.6

Microsome Isolation Kit
K249-50	Biovision	each	EUR 633.6

Preadipocyte Isolation Kit
K583-5	Biovision	each	EUR 639.6

ExoQuick Exosome Isolation and RNA Purification kit (for Serum and Plasma)
EQ806A-1	SBI	20 preps	EUR 664.8

ExoQuick Exosome Isolation and RNA Purification kit (for Tissue Culture Media)
EQ806TC-1	SBI	10 preps	EUR 664.8

Apoptotic Cell Isolation Kit
55R-1349	Fitzgerald	30 units	EUR 874.8
Description: Apoptotic Cell Isolation Kit for use in the research laboratory

Mitochondrial DNA Isolation Kit
55R-1361	Fitzgerald	50 assays	EUR 990
Description: Mitochondrial DNA Isolation Kit for use in the research laboratory

Genomic DNA Isolation Kit
55R-1362	Fitzgerald	50 assays	EUR 462
Description: Genomic DNA Isolation Kit for use in the research laboratory

Cell Mitochondria Isolation Kit
abx090630-50100tests	Abbexa	50-100 tests	EUR 309.6

Tissue Mitochondria Isolation Kit
abx090631-50100assays	Abbexa	50-100 assays	EUR 309.6

Lipid Droplet Isolation Kit
MET-5011	Cell Biolabs	50 preps	EUR 498
Description: Lipid droplets are lipid rich organelles found in the adipose tissue of eukaryotes. They function to regulate the hydrolysis and storage of neutral lipids and serve as storage for cholesterol and acyl-glycerols. Lipid droplets have also been associated with inflammatory responses, obesity, atherosclerosis, and cancer.

Mitochondrial DNA Isolation Kit
K2117-50	ApexBio	50 assays	EUR 718.8

Genomic DNA Isolation Kit
K2118-50	ApexBio	50 assays	EUR 385.2

Saliva DNA Isolation Kit
K5011050	Biochain	1 kit	EUR 307.2

Urine DNA Isolation Kit
K5011150	Biochain	1 kit	EUR 307.2

Stool DNA Isolation Kit
K5011250	Biochain	1 kit	EUR 307.2

Serum DNA Isolation Kit
K5018100	Biochain	1 kit	EUR 432

ExoPure? Isolation Kit   (Urine)
K1240-10	Biovision	each	EUR 836.4

ExoPure? Isolation Kit   (Urine)
K1240-2	Biovision	each	EUR 483.6

Apoptotic Cell Isolation Kit
K258-30	Biovision	each	EUR 561.6

Yeast Mitochondria Isolation Kit
K259-50	Biovision	each	EUR 502.8

Mitochondrial DNA Isolation Kit
K280-50	Biovision	each	EUR 705.6

Genomic DNA Isolation Kit
K281-50	Biovision	each	EUR 326.4

Yeast Nuclei Isolation Kit
K289-50	Biovision	each	EUR 470.4

Lipopolysaccharide (LPS) Isolation Kit
K438-10	Biovision	each	EUR 333.6

Frit Kit
FRIT-KIT	Next Advance	1each	EUR 148.8
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Column Packing Kit
PACK-KIT	Next Advance	1pack	EUR 1242
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

Exo-Urine EV Isolation Kit
EXOU100A-1	SBI	2 x 5 reactions	EUR 661.2

Easy3-Total NucleicAcid Isolation Kit
FYG504-100ML	Yeastern Biotech	100 ml	Ask for price

Easy3-Total NucleicAcid Isolation Kit
FYG504-500ML	Yeastern Biotech	500 ml	Ask for price

Circulating Nuleic Acid Isolation Kit
FACFK-050	Favorgen	50 preps	EUR 589.2

Circulating Nuleic Acid Isolation Kit
FACFK-100	Favorgen	100 preps	EUR 990

Genoirse Plant DNA Isolation Kit
GR101083	Genorise Scientific	50	EUR 267.6

Apoptotic DNA Ladder Isolation Kit
K2045-50	ApexBio	50 assays	EUR 602.4

XCF Exosomal DNA Isolation Kit
XCF200A-1	SBI	20 rxn	EUR 463.2

FitAmp Urine DNA Isolation Kit
P-1017-050	EpiGentek	50 Samples	EUR 171.6

FitAmp Urine DNA Isolation Kit
P-1017-100	EpiGentek	100 Samples	EUR 292.6

ChromaFlash Chromatin Isolation & Shearing Kit
P-2023-100	EpiGentek	100 Preparations	EUR 189.2

FitAmp Gel DNA Isolation Kit
P-1007	EpiGentek	100 Samples	EUR 324.48

FitAmp Urine DNA Isolation Kit
P-1017	EpiGentek	EUR 474.12 EUR 171.60 EUR 292.60	100 Samples 50 Samples 100 Samples

ChromaFlash Chromatin Isolation & Shearing Kit
P-2023	EpiGentek	EUR 324.48 EUR 189.20	100 Preperations 100 Preparations

ExoPure? Isolation Kit   (Cell Media)
K1237-10	Biovision	each	EUR 796.8

ExoPure? Isolation Kit   (Cell Media)
K1237-2	Biovision	each	EUR 470.4

ExoPure? Isolation Kit   (Serum, Plasma)
K1238-10	Biovision	each	EUR 836.4

ExoPure? Isolation Kit   (Serum, Plasma)
K1238-2	Biovision	each	EUR 470.4

ExoPure? Isolation Kit   (Bio Fluids)
K1241-10	Biovision	each	EUR 836.4

ExoPure? Isolation Kit   (Bio Fluids)
K1241-2	Biovision	each	EUR 483.6

Apoptotic DNA Ladder Isolation Kit
K170-50	Biovision	each	EUR 561.6

EZExtract? Core Histone Isolation Kit
K234-100	Biovision	each	EUR 314.4

Cell Surface Protein Isolation Kit
K295-10	Biovision	each	EUR 601.2

Bacterial Genomic DNA Isolation Kit
K309-100	Biovision	each	EUR 451.2

OrgFrontier? Plasma Membrane Isolation Kit
K414-10	Biovision	each	EUR 679.2

Org Frontier? Chloroplast Isolation Kit
K468-10	Biovision	each	EUR 666

Whole Blood DNA Isolation Kit
K528-100	Biovision	each	EUR 451.2

CD19 Positive Cell Isolation Kit
78564-1	BPS Bioscience	1 x 10^8 Cells	EUR 70
Description: The CD19 Positive Cell Isolation Kit is designed to magnetically separate CD19-expressing-cells from a complex immune cell population. This kit is optimized for the isolation of CD19 positive cells from normal human peripheral blood mononuclear cells (PBMCs). Cells are incubated with the antibody:bead complex and placed on a magnet for quick and easy separation. When placed on the magnet, CD19-positive cells will be immobilized along the side of the tube while undesired CD19-negative cells will remain in suspension for easy removal.

CD19 Positive Cell Isolation Kit
78564-2	BPS Bioscience	1 x 10^9 Cells	EUR 630
Description: The CD19 Positive Cell Isolation Kit is designed to magnetically separate CD19-expressing-cells from a complex immune cell population. This kit is optimized for the isolation of CD19 positive cells from normal human peripheral blood mononuclear cells (PBMCs). Cells are incubated with the antibody:bead complex and placed on a magnet for quick and easy separation. When placed on the magnet, CD19-positive cells will be immobilized along the side of the tube while undesired CD19-negative cells will remain in suspension for easy removal.

PCR Mycoplasma Detection Kit
M034-Kit	TOKU-E	Kit	EUR 319.2

Human anti-cartilage-antibody(anti-cartilage-Ab) ELISA Kit
YLA0056HU-48T	Shanghai YL Biotech	48T	EUR 435

Human anti-cartilage-antibody(anti-cartilage-Ab) ELISA Kit
YLA0056HU-96T	Shanghai YL Biotech	96T	EUR 562.5

Orange Lysis Kit 50
ORANGEE1-RNA	Next Advance	50pack	EUR 198
Description: Bead lysis kits for extracting bacteria or generating single cell suspensions from tissue samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Orange Lysis Kit 250
ORANGEE5-RNA	Next Advance	250pack	EUR 644.4
Description: Bead lysis kits for extracting bacteria or generating single cell suspensions from tissue samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Mouse Cartilage PrimaCell: Normal Articular Cartilage
2-82096	CHI Scientific	1 Kit	Ask for price

Rat Cartilage PrimaCell: Normal Articular Cartilage
2-82523	CHI Scientific	1 Kit	Ask for price

Human Cartilage PrimaCell: Normal Articular Cartilage
2-96043	CHI Scientific	1 Kit	Ask for price

PSEUDOMONAS ISOLATION AGAR
P16-114-10kg	Alphabiosciences	10 kg	EUR 2058

PSEUDOMONAS ISOLATION AGAR
P16-114-2kg	Alphabiosciences	2kg	EUR 494.4

PSEUDOMONAS ISOLATION AGAR
P16-114-500g	Alphabiosciences	500 g	EUR 177.6

PSEUDOMONAS ISOLATION BROTH
P16-115-10kg	Alphabiosciences	10 kg	Ask for price

PSEUDOMONAS ISOLATION BROTH
P16-115-2Kg	Alphabiosciences	2 Kg	Ask for price

PSEUDOMONAS ISOLATION BROTH
P16-115-500g	Alphabiosciences	500 g	Ask for price

Orange RINO Lysis Kit 50
ORANGER1-RNA	Next Advance	50pack	EUR 202.8
Description: Bead lysis kits for extracting bacteria or generating single cell suspensions from tissue samples. Pack of 50, 1.5mL RINO tubes prefilled with stainless steel beads. RNase free.

Orange RINO Lysis Kit 250
ORANGER5-RNA	Next Advance	250pack	EUR 664.8
Description: Bead lysis kits for extracting bacteria or generating single cell suspensions from tissue samples. Pack of 250, 1.5mL RINO tubes prefilled with stainless steel beads. RNase free.

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit
CAS400A-KIT	SBI	1 kit (10 rxn)	EUR 1332

Pesticide Kit Containing All 10 Multi-Compound Mixes
SPXPR-KIT	Scientific Laboratory Supplies	1ML	EUR 1923.18

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit
CAS410A-KIT	SBI	1 kit (10 rxn)	EUR 798

Cas9-EGFP Protein and T7 gRNA SmartNuclease Synthesis Kit
CAS420A-KIT	SBI	1 kit (10 rxn)	EUR 828

RNA Kit
20-abx098089	Abbexa	EUR 126.00 EUR 410.40	2 rxns 50 rxns

CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV100PA-KIT	SBI	1 Kit	EUR 1358.4

CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV105PA-KIT	SBI	1 Kit	EUR 1358.4

MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV120PA-KIT	SBI	1 Kit	EUR 1358.4

MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV125PA-KIT	SBI	1 Kit	EUR 1358.4

Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector
CAS700A-KIT	SBI	10 rxn	EUR 1358.4

Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector
CAS720A-KIT	SBI	10 rxn	EUR 1358.4

Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector
CAS740A-KIT	SBI	10 rxn	EUR 1358.4

Mammalian Mitochondria Isolation Kit for Tissue
abx098857-50rxns	Abbexa	50 rxns	EUR 493.2

FastPure Bacteria DNA Isolation Mini Kit
DC103	Vazyme	100 rxn	EUR 212.4

FastPure Plant DNA Isolation Mini Kit
DC104	Vazyme	50 rxn	EUR 192

Conclusions: We have developed a labeling and gating strategy strictly capable of exploring the eV marker expression (CD63, CD9 and LAMP1) on individual EV in a largely heterogeneous population. Taken together, the data presented here strongly support the value of the exploration of large EVS in clinical samples for potential biomarkers, useful in the monitoring of diagnosis and disease surveillance.