Mesenchymal stem cell derived-exosomes: a modern approach in translational medicine

Mesenchymal stem cells (MSCs) have captured great attention in regenerative treatment for more than decades based on their differentiation capacity, strong immunomodulator properties, and their ability to be kululiped and manipulated. The recent investigation implies that the MSCS pleiotropic effect is not related to their differentiation capabilities, but is more mediated by the secretion of soluble paracrine factors. Exosom, nano extracellular vesicles, is one of these paracrine mediators.

Exosom functional cargo transfers such as MIRNA and MRNA molecules, peptides, protein, cytokines, and lipids from MSc to the recipient’s cell. Exocomic participates in communication events between cells and contributes to healing tissues and injured organs. Studies report that exosom alone is responsible for the therapeutic effect of MSC in various experimental models. Therefore, the exosom derived MSC can be manipulated and applied to establish a new cell-free therapeutic approach to the treatment of various diseases including the heart, kidney, liver, immune, and neurological disease, and healing of skin wounds. Compared to their donor cells, the exosom derived MSC offers more stable entities and reduced risk of safety regarding living cell administration, eg. Microvascular occlusion risk.

This review discusses the exosome insulation method found and utilized in clinical settings so far and present the latest information summary of the MSC exosomes in the treatment of translation. Exosom is an extracellular type of vesicle (EVS) issued by several types of mammal cells and involved in communication between cells. A number of studies have explored the potential diagnostic and exosomal therapeutic. The main challenge is the lack of standard and standard techniques for insulation and analysis of downstream nanovesicles. Conventional insulation method, such as ultracentrification, precipitation, filtration, chromatography, and an immune affinity-based approach, depends on certain physical properties or in surface biomarkers. However, one of the existing methods has limitations. Various parameters, such as efficacy, specificity, labor input, cost and scalability, and standardization options, must be considered for the appropriate approach.

The communication of extracellular vesicles between mesenchymal stroma cells and immune effect cells

Mesenchymal Stem / Stromal Cell (MSCS) is a multiphotic cell that is on the body’s stroma network and is able to promote network repair and weaken the inflammatory process through its immunomodulatory properties. Preclinical and clinical observations reveal that it is not only inter-cell communication that directly mediates MSC property; In fact, an important role is also played by the release of soluble and bioactive factors, such as cytokines, growth factors and extracellular vesicles (EVs). EVS is a membrane plated vesicel that contains various kinds of bioactive molecules, including lipids, proteins, and nucleic acids, such as RNA.

EVS let go of their content into target cells, thus affecting the fate of cells through intracellular process control. In addition, EV which is revealed MSC can mediate the effect of modulation towards different effector cells belonging to innate and adaptive immunity. In this review, we will discuss the literature data on EVS obtained by MSC, including the current standard method for their isolation and characterization, the mechanism that supports its immunoregulator properties, and their potential clinical applications as an alternative MSc-based therapy for inflammatory reactions, such as grafting disease Versus-hosted (GVHD).

 Mesenchymal stem cell derived-exosomes: a modern approach in translational medicine
Mesenchymal stem cell derived-exosomes: a modern approach in translational medicine

Large extracellular vesicles can be characterized by multiplex labeling using imaging flow cytometry

The extracellular vesicles (EVS) are heterogeneous (30 Nm-10 μm), content (lipid, RNA, DNA, protein) and potential function (s). Many isolation techniques systematically throw large EVS in the early stages of the small or exosomatic isolation protocols. We describe here a standardized method for isolating large eV from medulloblastoma cells and examine the expression of the EV marker and the diameter using the imaging flow cytometry. Our approach allows the characterization of each large EVS as an individual event, decorated with several fluorescent conjugal markers with the additional advantage of visualizing each event to ensure an application of robust gage strategies.

Methods: We describe the isolation of the step and characterization of a subset of large EVS from the UW228-2 medulloblastoma cell line evaluated by fluorescent light microscopy, transmission electron microscopy (TEM) and Tunable resistance detection (TRP). The viability of parental cells was evaluated by the Annexine V exposure by flow cytometry. Imagery flow cytometry (ImageReam Mark II) identified EVS by direct fluorescent membrane marking with orange cell mask (CMO) in conjunction with the EV markers. A rigorous guarantee algorithm based on the intensity of lateral diffusion and fluorescence has been applied and the expression of the EV markers CD63, CD9 and the lamp 1 evaluated.

Results: UW228-2 cells extend prolifically EVS upwards up to 6 μm. We show that the Imaging Flow Cytometer II allows a robust and reproducible analysis of the large EVS, including the evaluation of the diameter. We also demonstrate a correlation between the increase in the size of the EV and the co-expression of the filtered markers.

Rapid Blood RNA Isolation Kit

BT4184 250preps
EUR 608.1

Total RNA Isolation Kit (Plant)

P-9104 50 samples
EUR 416.7

Rapid Plant RNA Isolation Kit

PT4191 50Preps
EUR 95.63

Total RNA Isolation Kit (Tissue)

P-9102 50 samples
EUR 416.7

EpiMag Viral RNA Isolation Kit

P-9108-050 50 samples
EUR 152.9

Easy3-Plant RNA Isolation Kit

FYG502-100P 100 Preps Ask for price

Easy3-Plant RNA Isolation Kit

FYG502-500P 500 Preps Ask for price

Rapid Bacteria RNA Isolation Kit

BS8625 50Preps
EUR 95.63

Broad Range Total RNA Isolation Kit

K1341050 1 kit
EUR 259

Phenol-Free Plant RNA Isolation Kit

10760056-1 50 Prep(s)
EUR 170.53

Easy3-RNA Isolation Kit

FYG501-100P 100 Preps Ask for price

Easy3-RNA Isolation Kit

FYG501-500P 500 Preps Ask for price

EpiQuik Magbeads Quick RNA Isolation Kit

P-9106-050 50 samples
EUR 163.9

Soil RNA Isolation Mini Kit (100prep)

FASRK-100 100 preps
EUR 224.4

Soil RNA Isolation Mini Kit (50prep)

FASRK-001 50 preps
EUR 180

EpiQuik Total RNA Isolation Fast Kit

P-9105-050 50 samples
EUR 176

EpiQuik Viral RNA Isolation Fast Kit

P-9107-050 50 samples
EUR 167.2

RNA-easy Isolation Reagent

R701-01 100 ml
EUR 201.6

RNA-easy Isolation Reagent

R701-02 200 ml
EUR 254.4

FastPure Cell/Tissue Total RNA Isolation Kit

RC101 50 rxn
EUR 274.8

Total RNA Isolation Kit II (Tri-reagent + RNA spin column)

FATRS__050 1 kit
EUR 62

Total RNA Isolation Kit II (Tri-reagent + RNA spin column)

FATRS__100 1 kit
EUR 112

FastPure Universal Plant Total RNA Isolation Kit

RC411-01 50 rxns
EUR 100

FastPure Cell/Tissue Total RNA Isolation Kit V2

RC112-01 50 rxns
EUR 82.6

miRNA Isolation Kit (Tri-reagent + RNA spin column)

FAMIK__001 1 kit
EUR 134.5

miRNA Isolation Kit (Tri-reagent + RNA spin column)

FAMIK__002 1 kit
EUR 73

PureXtract RNAsol RNA Isolation Solution, LS

R6103-120 2X60ml
EUR 343.2

miRNA Isolation Kit

FAMIK--001 Rxns: 100
EUR 398.4
Description: Contents of the kit:
  • Rxns:100Lysis
  • Buffer-10 ml
  • Acid-Phenol-10 ml
  • RNA Column and Collection Tube-100 sets
  • Wash Buffer-10 ml
  • Release Buffer-0.5 ml

miRNA Isolation Kit

FAMIK--002 Rxns: 50
EUR 267.6

miRNA Isolation Kit

FYG304-100P 100 Preps Ask for price

Exosome Isolation Kit

E601E001 20 tests
EUR 2500

MicroRNA Isolation Kit

KS341025 1 kit
EUR 247

Endosome Isolation Kit

P528 - Ask for price

Microsome Isolation Kit

K249-50 each
EUR 633.6

FastPure Plant Total RNA Isolation Kit (Polysaccharides&Polyphenolics –rich)

RC401-01 50 rxns
EUR 100

Protoplast Isolation Kit

30210002-1 20 Prep(s)
EUR 199.16

Protoplast Isolation Kit

30210002-2 50 Prep(s)
EUR 438.32

CELLDATA RNAstorm™ Fresh Cell and Tissue RNA Isolation Kit, 50 preps

CD504 1
EUR 270
Description: DNAse I from bovine pancreas (cow; Bos taurus)

Chloroplast Isolation Kit

30210000-1 20 Prep(s)
EUR 273.97

FastPure Plant Total RNA Isolation Kit (Polysaccharides/Polyphenolics-Rich)

RC401 50 rxn
EUR 283.2

Mitochondria Isolation Kit

AR0156 1 kit
EUR 127.2

Preadipocyte Isolation Kit

K583-5 each
EUR 639.6

Urine DNA Isolation Kit

K5011150 1 kit
EUR 237

Stool DNA Isolation Kit

K5011250 1 kit
EUR 237

Serum DNA Isolation Kit

K5018100 1 kit
EUR 355

Saliva DNA Isolation Kit

K5011050 1 kit
EUR 237

Genomic DNA Isolation Kit

55R-1362 50 assays
EUR 462
Description: Genomic DNA Isolation Kit for use in the research laboratory

Genomic DNA Isolation Kit

K281-50 each
EUR 326.4

Genomic DNA Isolation Kit

K2118-50 50 assays
EUR 385.2

Yeast Nuclei Isolation Kit

K289-50 each
EUR 470.4

Lipid Droplet Isolation Kit

MET-5011 50 preps
EUR 498
Description: Lipid droplets are lipid rich organelles found in the adipose tissue of eukaryotes. They function to regulate the hydrolysis and storage of neutral lipids and serve as storage for cholesterol and acyl-glycerols. Lipid droplets have also been associated with inflammatory responses, obesity, atherosclerosis, and cancer.

ExoPure? Isolation Kit   (Urine)

K1240-10 each
EUR 836.4

ExoPure? Isolation Kit   (Urine)

K1240-2 each
EUR 483.6

Apoptotic Cell Isolation Kit

55R-1349 30 units
EUR 874.8
Description: Apoptotic Cell Isolation Kit for use in the research laboratory

Apoptotic Cell Isolation Kit

K258-30 each
EUR 561.6

miRNA ISOLATION KIT-TISSUE

IB47370 4 PREP KIT
EUR 30.42

miRNA ISOLATION KIT-TISSUE

IB47371 100 PREP KIT
EUR 579.66

Exosome Isolation kit (for serum & plasma -Exosome-RNA-NGS)

P101NGS - Ask for price

Exo-Urine EV Isolation Kit

EXOU100A-1 2 x 5 reactions
EUR 490

FitAmp Gel DNA Isolation Kit

P-1007 100 Samples
EUR 324.48

Cell Mitochondria Isolation Kit

abx090630-50100tests 50-100 tests
EUR 309.6

Mitochondrial DNA Isolation Kit

55R-1361 50 assays
EUR 990
Description: Mitochondrial DNA Isolation Kit for use in the research laboratory

Mitochondrial DNA Isolation Kit

K280-50 each
EUR 705.6

Mitochondrial DNA Isolation Kit

K2117-50 50 assays
EUR 718.8

Mitochondrial DNA Isolation Kit

K2117-1 1 kit
EUR 858
Description: Isolates mt. DNA, no genomic DNA contamination

Cell Mitochondria Isolation Kit

ETF3601 100 assays
EUR 135

Whole Blood DNA Isolation Kit

K528-100 each
EUR 451.2

Plant/Fungi DNA Isolation Kit

10760114-1 50 Prep(s)
EUR 350.39

Yeast Mitochondria Isolation Kit

K259-50 each
EUR 502.8

FitAmp Urine DNA Isolation Kit 

P-1017
  • EUR 474.12
  • EUR 171.60
  • EUR 292.60
  • 100 Samples
  • 50 Samples
  • 100 Samples

XCF Exosomal DNA Isolation Kit

XCF200A-1 20 rxn
EUR 328

Tissue Mitochondria Isolation Kit

abx090631-50100assays 50-100 assays
EUR 309.6

Tissue Mitochondria Isolation Kit

ETF3606 100 assays
EUR 135

Lysosome Isolation Kit (20 tests)

P534 - Ask for price

Exosome Isolation kit (for urine)

P120L - Ask for price

Exosome Isolation kit (for urine)

P120S - Ask for price

FastPure FFPE DNA Isolation Kit

DC105-01 50 rxns
EUR 75.2

DNA/RNA Mini Kit (isolation of both genomic DNA and total RNA from a single sample)

FADRK_050 1 kit
EUR 90

CD19 Positive Cell Isolation Kit

78564-1 1 x 10^8 Cells
EUR 70
Description: The CD19 Positive Cell Isolation Kit is designed to magnetically separate CD19-expressing-cells from a complex immune cell population. This kit is optimized for the isolation of CD19 positive cells from normal human peripheral blood mononuclear cells (PBMCs). Cells are incubated with the antibody:bead complex and placed on a magnet for quick and easy separation. When placed on the magnet, CD19-positive cells will be immobilized along the side of the tube while undesired CD19-negative cells will remain in suspension for easy removal.

CD19 Positive Cell Isolation Kit

78564-2 1 x 10^9 Cells
EUR 630
Description: The CD19 Positive Cell Isolation Kit is designed to magnetically separate CD19-expressing-cells from a complex immune cell population. This kit is optimized for the isolation of CD19 positive cells from normal human peripheral blood mononuclear cells (PBMCs). Cells are incubated with the antibody:bead complex and placed on a magnet for quick and easy separation. When placed on the magnet, CD19-positive cells will be immobilized along the side of the tube while undesired CD19-negative cells will remain in suspension for easy removal.

Genoirse Plant DNA Isolation Kit

GR101083 50
EUR 223

ExoPure? Isolation Kit   (Cell Media)

K1237-10 each
EUR 796.8

ExoPure? Isolation Kit   (Cell Media)

K1237-2 each
EUR 470.4

ExoPure? Isolation Kit   (Bio Fluids)

K1241-10 each
EUR 836.4

ExoPure? Isolation Kit   (Bio Fluids)

K1241-2 each
EUR 483.6

Lipopolysaccharide (LPS) Isolation Kit

K438-10 each
EUR 333.6

ExoPure? Isolation Kit   (Serum, Plasma)

K1238-10 each
EUR 836.4

ExoPure? Isolation Kit   (Serum, Plasma)

K1238-2 each
EUR 470.4

Apoptotic DNA Ladder Isolation Kit

K170-50 each
EUR 561.6

Cell Surface Protein Isolation Kit

K295-10 each
EUR 601.2

Apoptotic DNA Ladder Isolation Kit

K2045-50 50 assays
EUR 602.4

GPC1 Capture Exosome Isolation Kit

E601E004 20 tests
EUR 2500

CD63 Capture Exosome Isolation Kit

E601E005 20 tests
EUR 2500

CD81 Capture Exosome Isolation Kit

E601E006 20 tests
EUR 2500

Blood and Serum DNA Isolation Kit

K5017100 1 kit
EUR 355

Bacterial Genomic DNA Isolation Kit

K309-100 each
EUR 451.2

Breast cancer Exosome Isolation Kit

E601E002 20 tests
EUR 2500

EPCAM Capture Exosome Isolation Kit

E601E003 20 tests
EUR 2500

HSP70 Capture Exosome Isolation Kit

E601E007 20 tests
EUR 2500

EZExtract? Core Histone Isolation Kit

K234-100 each
EUR 314.4

10-min. Single Cell Isolation Kit

P713-20 - Ask for price

MagIso NK Cell Isolation Kit, Human

WHK-B023 20 tests, 200 tests Ask for price

Circulating Nuleic Acid Isolation Kit

FACFK-050 50 preps
EUR 589.2

Circulating Nuleic Acid Isolation Kit

FACFK-100 100 preps
EUR 990

Detergent-Free Nuclei Isolation Kit

P524-2 - Ask for price

Detergent-Free Nuclei Isolation Kit

P524-20 - Ask for price

Detergent-Free Nuclei Isolation Kit

P524-80 - Ask for price

Mouse Brain Microvessel Isolation Kit

P750-4 - Ask for price

Circulating Nuleic Acid Isolation Kit

FACFK_050 1 kit
EUR 476

MagIso CD3 Cell Isolation Kit, Mouse

WHK-B009 20 tests, 200 tests Ask for price

Org Frontier? Chloroplast Isolation Kit

K468-10 each
EUR 666

Nucleic fluid extraction isolation kit

K1137-100 100rxns
EUR 160
Description: Nucleic fluid extraction isolation kit

Nucleic fluid extraction isolation kit

K1137-200 200rxns
EUR 270
Description: Nucleic fluid extraction isolation kit

Nucleic fluid extraction isolation kit

K1137-50 50rxns
EUR 90
Description: Nucleic fluid extraction isolation kit

MagIso CD90.2 Cell Isolation Kit, Mouse

WHK-B010 20 tests, 200 tests Ask for price

ExoQuick Exosome Isolation and RNA Purification kit (for Serum and Plasma)

EQ806A-1 20 preps
EUR 493

Easy3-Total NucleicAcid Isolation Kit

FYG504-100ML 100 ml Ask for price

Easy3-Total NucleicAcid Isolation Kit

FYG504-500ML 500 ml Ask for price

FitAmp Plasma/Serum DNA Isolation Kit 

P-1004
  • EUR 416.70
  • EUR 149.60
  • EUR 253.00
  • 100 Samples
  • 50 Samples
  • 100 Samples

ExoPure? Isolation Kit   (Stem Cell Media)

K1239-10 each
EUR 901.2

ExoPure? Isolation Kit   (Stem Cell Media)

K1239-2 each
EUR 510

Exosome Isolation kit (for serum & plasma)

P101 - Ask for price

Exosome Isolation kit (for serum & plasma)

P101L - Ask for price

Exosome Isolation kit (for serum & plasma)

P101S - Ask for price

Rapid Fungal Genomic DNA Isolation Kit

FT71415 50Preps
EUR 91.97

Plant Tissue Genomic DNA Isolation Kit

K316-100 each
EUR 451.2

MagIso CX3CR1 Cell Isolation Kit, Mouse

WHK-B017 10 tests, 100 tests Ask for price

OrgFrontier? Plasma Membrane Isolation Kit

K414-10 each
EUR 679.2

Mitochondria Isolation Kit (Cultured Cells)

K1138-10 10rxns
EUR 35
Description: Mitochondria Isolation Kit

Mitochondria Isolation Kit (Cultured Cells)

K1138-20 20rxns
EUR 55
Description: Mitochondria Isolation Kit

Mitochondria Isolation Kit (Cultured Cells)

K1138-30 30rxns
EUR 70
Description: Mitochondria Isolation Kit

Easy3-Plant Genomic DNA Isolation Kit

FYG503-100P 100 Preps Ask for price

Easy3-Plant Genomic DNA Isolation Kit

FYG503-500P 500 Preps Ask for price

MagIso CD4 T Cell Isolation Kit, Human

WHK-B001 10 tests
EUR 724.8

MagIso CD8 T Cell Isolation Kit, Human

WHK-B002 10 tests
EUR 724.8

MagIso CD4 T Cell Isolation Kit, Mouse

WHK-B003 10 tests
EUR 724.8

MagIso CD8 T Cell Isolation Kit, Mouse

WHK-B004 10 tests
EUR 724.8

MagIso Pan B Cell Isolation Kit, Mouse

WHK-B013 20 tests, 200 tests Ask for price

MagIso Pan B Cell Isolation Kit, Human

WHK-B022 20 tests, 200 tests Ask for price

Rapid Bacteria Genomic DNA Isolation Kit

BS8225 50Preps
EUR 91.97

Conclusions: We have developed a labeling and gating strategy strictly capable of exploring the eV marker expression (CD63, CD9 and LAMP1) on individual EV in a largely heterogeneous population. Taken together, the data presented here strongly support the value of the exploration of large EVS in clinical samples for potential biomarkers, useful in the monitoring of diagnosis and disease surveillance.