Exosomes isolated from two different cell lines using three different isolation techniques show variation in physical and molecular characteristics

Exosom is an extracellular vesicel bi-lipid nanoscopic lipid with the potential to be used as a targeted therapy. In our investigation, we compare three main exosome insulation techniques which are total exosome insulation reagents (TEIs), protein precipitation of organic solvents (PROSPR) and differential ultracentrification (UC) based on biophysical and physicochemical characteristics of exosoms isolated from Colo 205 and MCF-7 Media is conditioned by cancer cells with the aim of choosing the appropriate method for translational studies. Analysis of 3D images and the distribution of the size of the exosomal particle of the image of their hrtem describes morphological differences.

Molecular and analytic characterization of exosomes uses Western Blotting, Raman and ATR-FTIR spectroscopy and multivariate analysis in spectral data obtained, assessed for better molecular specifications and particle purity. The Exocomic Insulated TEI Method with higher exosomal results, purity, and recovery that directly translates into targeted drugs and therapies while Ultracentrifuge has good particle morphological recovery but shows a smaller average size particle aggregation. ProSPI technique isolates the EVS mixture, indicating lower protein recovery in pages and western bloting but higher spectroscopic proteins on lipid ratios and EV populations that can be distinguished in multivariate analysis compared to exosoms isolated by ex. This comparative study must help in choosing the specific exosomatic insulation techniques needed for downstream application purposes.

 

Exosom in cardiovascular disease

Ecosomes are bioveicles of nano-sized nano origin physiologically released by almost all types of cells into surrounding body fluids. They carry protein cargo, lipids, and special genetics of cells and can be selectively taken by neighboring cells or far away. Because the intrinsic nature of the exosomes is greatly influenced by the state of the cells of parents and by the cellular micro environment, the origin and content analysis of the exosomes, and the specificity of their cell targeting, making it interesting as a possible diagnostic biomarker and prognostic. While the possibility of the exosom role as a messenger and regenerative tool in cardiovascular disease (CVD) is actively investigated, evidence of its usefulness as biomarkers is still limited and incomplete.

Further complications are caused by the lack of consensus regarding the most appropriate approach to ecosome isolation and characterization, both important problems for effective clinical translations. As a consequence, in this review, we will discuss some information that is currently accessible to the diagnostic / prognostic potential of the exosomes in the CVD and on the methodology available for ecosome isolation, analysis, and characterization. Exosom is an important group of extracellular vesicles. They are formed in the endosomal compartment and actively secreted in extracellular space. Some membrane proteins, including Integrin, are present on the surface of the exosom.

Because integrin exosomes are competent to bind the ligand, they can play an important role in directing the exosom network distribution. Integrin exosomal trade directed at Vivo is involved in regulating renovation of cell homing niches for cancer metastasis and migration lymphocytes. This chapter explains the methods used to study integrin functions in exosom including: isolation and characterization of exosomal biophysome, binding exosomal integrin-ligands, and in the competitive excosom homing test of Vivo.

 Exosomes isolated from two different cell lines using three different isolation techniques show variation in physical and molecular characteristics
Exosomes isolated from two different cell lines using three different isolation techniques show variation in physical and molecular characteristics

Optical, electrochemical and electrical (Nano) biosensors for the detection of exosomes: a complete overview.

The exosomes are small extracellular vesicles involved in many physiological activities of cells in the human body. The exosoms of cancer cells have great application potential in clinical diagnosis, anticipated cancer detection and target identification for molecular therapy. Although this area is gaining increased interests of the academy and industry, barriers such as superse sensing techniques and highly efficient isolation methods remain. In clinical settings, there is an urgent need for rapid analysis, reliable detection and point of care test (POCT). With these challenges, this article aims to examine recent developments and technical advances, including optical, electrochemical and electrical biosensors for the detection of exosomes in the field of cancer and other diseases and demonstrate how nanobiosensters could improve

The performance of conventional sensors. Work strategies, the limit of detections, benefits and gaps of studies are summarized. The new trends, challenges and future prospects of the exosomatic OPP in liquid biopsy were discussed. Adipocytes associated with cancer have functional roles in the development of the tumor thanks to factors and exosomes derived from secreted adipocytes as well as by metabolic symbiosis, where malignant cells take lactate, fatty acids and glutamine produced by the neighboring adipocytes. Recent research has demonstrated the value of adipocytes as cell-based distribution platforms for drugs (or prodrugs), nucleic acids or nanoparticles loaded for cancer therapy.

Actinobacillus actinomycetemcomitans

Oneq-H446-OneqH446100D Oneq-H446-100D
EUR 515

Actinobacillus actinomycetemcomitans

Oneq-H446-OneqH446150D Oneq-H446-150D
EUR 594

Actinobacillus actinomycetemcomitans

Oneq-H446-OneqH44650D Oneq-H446-50D
EUR 413

Actinobacillus actinomycetemcomitans PCR kit

PCR-H446-48D 50T
EUR 543.6

Actinobacillus actinomycetemcomitans PCR kit

PCR-H446-96D 100T
EUR 686.4

Actinobacillus actinomycetemcomitans RT PCR kit

RTq-H446-100D 100T
EUR 860.4

Actinobacillus actinomycetemcomitans RT PCR kit

RTq-H446-150D 150T
EUR 969.6

Actinobacillus actinomycetemcomitans RT PCR kit

RTq-H446-50D 50T
EUR 717.6

Actinobacillus actinomycetemcomitans One-Step PCR kit

Oneq-H446-100D 100T
EUR 1039.2

Actinobacillus actinomycetemcomitans One-Step PCR kit

Oneq-H446-150D 150T
EUR 1177.2

Actinobacillus actinomycetemcomitans One-Step PCR kit

Oneq-H446-50D 50T
EUR 861.6

Aggregatibacter actinomycetemcomitans PCR kit

PCR-H710-48D 50T
EUR 543.6

Aggregatibacter actinomycetemcomitans PCR kit

PCR-H710-96D 100T
EUR 686.4

Aggregatibacter actinomycetemcomitans RT PCR kit

RTq-H710-100D 100T
EUR 860.4

Aggregatibacter actinomycetemcomitans RT PCR kit

RTq-H710-150D 150T
EUR 969.6

Aggregatibacter actinomycetemcomitans RT PCR kit

RTq-H710-50D 50T
EUR 717.6

Aggregatibacter actinomycetemcomitans One-Step PCR kit

Oneq-H710-100D 100T
EUR 1039.2

Aggregatibacter actinomycetemcomitans One-Step PCR kit

Oneq-H710-150D 150T
EUR 1177.2

Aggregatibacter actinomycetemcomitans One-Step PCR kit

Oneq-H710-50D 50T
EUR 861.6

Aggregatibacter actinomycetemcomitans Leukotoxin (ltxA)

1-CSB-YP322853AYK
  • EUR 814.80
  • EUR 402.00
  • EUR 2606.40
  • EUR 1261.20
  • EUR 1730.40
  • EUR 522.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Aggregatibacter actinomycetemcomitans Leukotoxin(ltxA),partial expressed in Yeast

Rna Isolation Kit

11828665001 EACH
EUR 378.62

miRNA Isolation Kit

FAMIK--001 Rxns: 100
EUR 398.4
Description: Contents of the kit:
  • Rxns:100Lysis
  • Buffer-10 ml
  • Acid-Phenol-10 ml
  • RNA Column and Collection Tube-100 sets
  • Wash Buffer-10 ml
  • Release Buffer-0.5 ml

miRNA Isolation Kit

FAMIK--002 Rxns: 50
EUR 267.6

miRNA Isolation Kit

FYG304-100P 100 Preps Ask for price

Exosome Isolation Kit

E601E001 20 tests
EUR 2500

MicroRNA Isolation Kit

KS341025 1 kit
EUR 247

Endosome Isolation Kit

P528 - Ask for price

Microsome Isolation Kit

K249-50 each
EUR 633.6

Protoplast Isolation Kit

30210002-1 20 Prep(s)
EUR 199.16

Protoplast Isolation Kit

30210002-2 50 Prep(s)
EUR 438.32

Recombinant Aggregatibacter actinomycetemcomitans Leukotoxin (ltxA) ,partial

RPC25117-100ug 100ug
EUR 817.3

Recombinant Aggregatibacter actinomycetemcomitans Leukotoxin (ltxA) ,partial

RPC25117-1mg 1mg
EUR 3461.9

Recombinant Aggregatibacter actinomycetemcomitans Leukotoxin (ltxA) ,partial

RPC25117-20ug 20ug
EUR 479.6

Chloroplast Isolation Kit

30210000-1 20 Prep(s)
EUR 273.97

Urine DNA Isolation Kit

K5011150 1 kit
EUR 237

Stool DNA Isolation Kit

K5011250 1 kit
EUR 237

Serum DNA Isolation Kit

K5018100 1 kit
EUR 355

Total RNA Isolation Kit

P-9100 50 samples
EUR 416.7

Mitochondria Isolation Kit

AR0156 1 kit
EUR 127.2

Preadipocyte Isolation Kit

K583-5 each
EUR 639.6

Chlamydia Isolation Medium

10-340100 100 mL Ask for price
Description: use with Chlamydial cell cultures

Chlamydia Isolation Medium

10-340500 500 mL Ask for price
Description: use with Chlamydial cell cultures

Saliva DNA Isolation Kit

K5011050 1 kit
EUR 237

PSEUDOMONAS ISOLATION BROTH

P16-115-10kg 10 kg Ask for price

PSEUDOMONAS ISOLATION BROTH

P16-115-2Kg 2 Kg Ask for price

PSEUDOMONAS ISOLATION BROTH

P16-115-500g 500 g Ask for price

10x Cell Isolation Buffer

78563 5 ml
EUR 25
Description: This buffer is intended for use with mammalian cells during magnetic cell isolation or single cell processing. This buffer is optimized and formulated to reduce nonspecific binding and cell clumping

Genomic DNA Isolation Kit

55R-1362 50 assays
EUR 462
Description: Genomic DNA Isolation Kit for use in the research laboratory

Easy3-RNA Isolation Kit

FYG501-100P 100 Preps Ask for price

Easy3-RNA Isolation Kit

FYG501-500P 500 Preps Ask for price

Genomic DNA Isolation Kit

K281-50 each
EUR 326.4

Genomic DNA Isolation Kit

K2118-50 50 assays
EUR 385.2

Yeast Nuclei Isolation Kit

K289-50 each
EUR 470.4

Lipid Droplet Isolation Kit

MET-5011 50 preps
EUR 498
Description: Lipid droplets are lipid rich organelles found in the adipose tissue of eukaryotes. They function to regulate the hydrolysis and storage of neutral lipids and serve as storage for cholesterol and acyl-glycerols. Lipid droplets have also been associated with inflammatory responses, obesity, atherosclerosis, and cancer.

ExoPure? Isolation Kit   (Urine)

K1240-10 each
EUR 836.4

ExoPure? Isolation Kit   (Urine)

K1240-2 each
EUR 483.6

Cartilage RNA Isolation Kit

K2031010 1 kit
EUR 443

Total RNA Isolation Kit II

FATRS--001 100 preps
EUR 369.6

Total RNA Isolation Kit II

FATRS--050 50 preps
EUR 259.2

EVery EV RNA Isolation Kit

EVery100B-1 20 preps
EUR 412

Apoptotic Cell Isolation Kit

55R-1349 30 units
EUR 874.8
Description: Apoptotic Cell Isolation Kit for use in the research laboratory

Apoptotic Cell Isolation Kit

K258-30 each
EUR 561.6

RNA-easy Isolation Reagent

R701-01 100 ml
EUR 201.6

RNA-easy Isolation Reagent

R701-02 200 ml
EUR 254.4

miRNA ISOLATION KIT-TISSUE

IB47370 4 PREP KIT
EUR 30.42

miRNA ISOLATION KIT-TISSUE

IB47371 100 PREP KIT
EUR 579.66

Custom cell exosome isolation

S101 -
EUR 700

Exo-Urine EV Isolation Kit

EXOU100A-1 2 x 5 reactions
EUR 490

FitAmp Gel DNA Isolation Kit

P-1007 100 Samples
EUR 324.48

Albumin, cold alcohol isolation

A0903 1g
EUR 99.67

Disease cell exosome isolation

S102 - Ask for price

Rapid Blood RNA Isolation Kit

BT4182 10Preps
EUR 98.36

Rapid Blood RNA Isolation Kit

BT4183 50Preps
EUR 197.03

Rapid Blood RNA Isolation Kit

BT4184 250preps
EUR 608.1

Whole Blood DNA Isolation Kit

K528-100 each
EUR 451.2

Total RNA Isolation Kit (Plant)

P-9104 50 samples
EUR 416.7

Rapid Plant RNA Isolation Kit

PT4191 50Preps
EUR 95.63

Plant/Fungi DNA Isolation Kit

10760114-1 50 Prep(s)
EUR 350.39

Cell Mitochondria Isolation Kit

abx090630-50100tests 50-100 tests
EUR 309.6

Mitochondrial DNA Isolation Kit

55R-1361 50 assays
EUR 990
Description: Mitochondrial DNA Isolation Kit for use in the research laboratory

Kinesis Rheodyne Isolation Seal

CHR2645 EACH
EUR 27.6

Kinesis Rheodyne Isolation Seal

CHR2791 EACH
EUR 46.8

Mitochondrial DNA Isolation Kit

K280-50 each
EUR 705.6

Mitochondrial DNA Isolation Kit

K2117-50 50 assays
EUR 718.8

Mitochondrial DNA Isolation Kit

K2117-1 1 kit
EUR 858
Description: Isolates mt. DNA, no genomic DNA contamination

Cell Mitochondria Isolation Kit

ETF3601 100 assays
EUR 135

FitAmp Urine DNA Isolation Kit 

P-1017
  • EUR 474.12
  • EUR 171.60
  • EUR 292.60
  • 100 Samples
  • 50 Samples
  • 100 Samples

Total RNA Isolation Kit (Tissue)

P-9102 50 samples
EUR 416.7

EpiMag Viral RNA Isolation Kit

P-9108-050 50 samples
EUR 152.9

XCF Exosomal DNA Isolation Kit

XCF200A-1 20 rxn
EUR 328

Yeast Mitochondria Isolation Kit

K259-50 each
EUR 502.8

cfMAX™ cfDNA Isolation System

CFMAX100A-1 1 Kit
EUR 412

Lysosome Isolation Kit (20 tests)

P534 - Ask for price

Exosome Isolation kit (for urine)

P120L - Ask for price

Exosome Isolation kit (for urine)

P120S - Ask for price

Easy3-Plant RNA Isolation Kit

FYG502-100P 100 Preps Ask for price

Easy3-Plant RNA Isolation Kit

FYG502-500P 500 Preps Ask for price

FastPure FFPE DNA Isolation Kit

DC105-01 50 rxns
EUR 75.2

Tissue Mitochondria Isolation Kit

abx090631-50100assays 50-100 assays
EUR 309.6

Tissue Mitochondria Isolation Kit

ETF3606 100 assays
EUR 135

CD19 Positive Cell Isolation Kit

78564-1 1 x 10^8 Cells
EUR 70
Description: The CD19 Positive Cell Isolation Kit is designed to magnetically separate CD19-expressing-cells from a complex immune cell population. This kit is optimized for the isolation of CD19 positive cells from normal human peripheral blood mononuclear cells (PBMCs). Cells are incubated with the antibody:bead complex and placed on a magnet for quick and easy separation. When placed on the magnet, CD19-positive cells will be immobilized along the side of the tube while undesired CD19-negative cells will remain in suspension for easy removal.

CD19 Positive Cell Isolation Kit

78564-2 1 x 10^9 Cells
EUR 630
Description: The CD19 Positive Cell Isolation Kit is designed to magnetically separate CD19-expressing-cells from a complex immune cell population. This kit is optimized for the isolation of CD19 positive cells from normal human peripheral blood mononuclear cells (PBMCs). Cells are incubated with the antibody:bead complex and placed on a magnet for quick and easy separation. When placed on the magnet, CD19-positive cells will be immobilized along the side of the tube while undesired CD19-negative cells will remain in suspension for easy removal.

Rapid Bacteria RNA Isolation Kit

BS8625 50Preps
EUR 95.63

Genoirse Plant DNA Isolation Kit

GR101083 50
EUR 223

ExoPure? Isolation Kit   (Cell Media)

K1237-10 each
EUR 796.8

ExoPure? Isolation Kit   (Cell Media)

K1237-2 each
EUR 470.4

ExoPure? Isolation Kit   (Bio Fluids)

K1241-10 each
EUR 836.4

ExoPure? Isolation Kit   (Bio Fluids)

K1241-2 each
EUR 483.6

Cell Isolation Magnetic Tube Rack

78571 15 ml and 50 ml
EUR 850
Description: This Cell Isolation Magnetic Tube Rack is designed to accompany our magnetic cell isolation products, such as the CD19 Positive Cell Isolation Kit (BPS Bioscience #78564). This magnet can quickly separate cells labeled with magnetic beads and is designed to allow users a near 360° view of their cell samples during separation. This magnet can accommodate both 15 and 50 ml tubes simultaneously, which allows flexibility for scaling up or down your isolation samples. Additionally, the rack is compatible with both conical and round-bottom tubes. The compact nature of this magnet allows it to fit easily in various incubators or refrigerators to match desired conditions.

Plasmid DNA Isolation Solution I

10760028-1 250 mL
EUR 29.83

Plasmid DNA Isolation Solution I

10760028-2 500 mL
EUR 45.3

Plasmid DNA Isolation Solution II

10760029-1 250 mL
EUR 29.83

Plasmid DNA Isolation Solution II

10760029-2 500 mL
EUR 37.89

Blood and Serum DNA Isolation Kit

K5017100 1 kit
EUR 355

ExoPure? Isolation Kit   (Serum, Plasma)

K1238-10 each
EUR 836.4

ExoPure? Isolation Kit   (Serum, Plasma)

K1238-2 each
EUR 470.4

Apoptotic DNA Ladder Isolation Kit

K170-50 each
EUR 561.6

Cell Surface Protein Isolation Kit

K295-10 each
EUR 601.2

Apoptotic DNA Ladder Isolation Kit

K2045-50 50 assays
EUR 602.4

Plasmid DNA Isolation Solution III

10760030-1 250 mL
EUR 28.07

Plasmid DNA Isolation Solution III

10760030-2 500 mL
EUR 43

GPC1 Capture Exosome Isolation Kit

E601E004 20 tests
EUR 2500

CD63 Capture Exosome Isolation Kit

E601E005 20 tests
EUR 2500

CD81 Capture Exosome Isolation Kit

E601E006 20 tests
EUR 2500

Lipopolysaccharide (LPS) Isolation Kit

K438-10 each
EUR 333.6

Bacterial Genomic DNA Isolation Kit

K309-100 each
EUR 451.2

SmartSEC Single EV Isolation System

SSEC200A-1 10 rxn
EUR 391

This strategy benefits from the biocompatibility of the delivery system, its ability to locate the site of the tumor and the predisposition of cancer cells to come into functional contact with adipocytes of tumor microenvironment for metabolic subsistence. In addition, their exosomal content can be used in the context of the reprogramming of cancer stem cells or as a delivery vehicle for different businesses, such as non-coding nucleic acids.