Optimization of microRNA Acquirement from Seminal Plasma and Identification of Diminished Seminal microRNA-34b as Indicator of Low Semen Concentration

Approximately 10-15% of couples who want to conceive suffer from subfertility, while in 30% of these cases, male factors play a role. The level of specific microRNAs in seminal plasma, including those involved in spermatogenesis, can serve as a parameter indicative of subfertility. We first optimized protocols to obtain microRNAs from seminal plasma.

Next, using a test strategy is validated in a group of men, we aim to identify microRNAs whose levels associated with semen motility and concentration. By qPCR, 742 microRNAs are profiled in normozoospermic three samples, three samples of sperm and low sperm motility (asthenozoospermia), and three with semen low concentration (oligozoospermia). MicroRNAs show a significant difference between the groups further validated in a second group consisting of 40 samples with normozoospermia (control group), 47 samples with asthenozoospermia, and 19 samples with oligozoospermia (which is 74% too low motility).

MicroRNA highest results obtained with biofluids RNA extraction kits, with the inclusion of MS2 RNA and proteinase K treatment operator for the protocol, and when 50 uL seminal plasma is used as input. Exosome RNA isolation before extraction does not lead to improved results. In the test cohort, 236 microRNAs can be detected, of which 54 microRNAs showed differences between groups. Five microRNAs analyzed in the validation group. the level of MiR-34b-5p in the control group was significantly higher compared with asthenozoospermia group (p <0.05) and compared with oligozoospermia group (p <0.001).

We optimized the acquisition of seminal plasma microRNA and microRNA levels were identified in relation to sperm concentration and motility. As the latest human and rat studies showed that miR-34 family is a marker of low concentration of semen and is very important in spermatogenesis, seminal plasma miR-34b-5p may be a suitable candidate for further study as a marker of male subfertility.

 Optimization of microRNA Acquirement from Seminal Plasma and Identification of Diminished Seminal microRNA-34b as Indicator of Low Semen Concentration
Optimization of microRNA Acquirement from Seminal Plasma and Identification of Diminished Seminal microRNA-34b as Indicator of Low Semen Concentration

When Less Is More: Special Capture and Analysis in the Plasma Tumor Exosom Liquid Biopsy Improves Sensitivity for Detection of Multiple androgen receptor Comprehensive Phenotypes in Advanced Prostate Cancer Patients

We evaluate the advantages and reliability of new protocols for enriching tumor extracellular vesicles (EV), allowing blood-based test for noninvasive parallel profiles of some of the androgen receptor (AR) gene changes. Three AR clinically relevant variants associated with response / resistance to standard-of-care treatment (AR-V7 transcripts, a point mutation AR T878A and AR gene amplification) were evaluated by digital PCR in 15 samples from patients affected by Castration-Resistant Prostate Cancer (CRPC).

Plasma is processed to obtain circulating RNA and DNA using protocols based enrichment EVS tumor through immuno-affinity and peptide-affinity compared with the generic extraction kit. Our results indicate that the immuno-affinity enrichment before RNA extraction clearly exceed the generic isolation method in the detection of the AR-V7, also allow for the differences between the respondents (R) and non-responders (NR) patients.The T878A mutation is detected, as a whole, in nine out of 15 samples, and no one approach alone is capable of expressing the mutation in all samples store, indicating that the method employed complementary.

Lentivirus Precipitation Solution

VC125 250 ml
EUR 432.6

Lentivirus Precipitation Solution

VC150 500 ml
EUR 814.8

Retrovirus Precipitation Solution

VC200 100 ml
EUR 194.04

Retrovirus Precipitation Solution

VC225 250 ml
EUR 432.6

Retrovirus Precipitation Solution

VC250 500 ml
EUR 814.8

PEG-it Virus Precipitation Solution (250 mL aliquot)

LV825A-1 250 ml
EUR 711

PEG-it Virus Precipitation Solution (100 mL aliquot)

LV810A-1 100 ml
EUR 325

ExoQuick Exosome RNA Column Purification kit

EQ808A-1 20 preps
EUR 344

TMB for Precipitation

42R-TB100x 1000 ml
EUR 645.6
Description: High sensitivity TMB Substrate for Precipitation assays

DNA Precipitation Buffer

D106 55 ml
EUR 210
Description: DNA Precipitation Buffer by Cygnus Technologies is available in Europe via Gentaur.

ExoQuick-LP for lipoprotein pre-clear & exosome isolation

EXOLP5A-1 5 reactions
EUR 473

PEG Virus Precipitation Kit

K904-200 each
EUR 1201.2

PEG Virus Precipitation Kit

K904-50 each
EUR 451.2

PEG Virus Precipitation Kit

K2190-200 200 preparations
EUR 1270.8

PEG Virus Precipitation Kit

K2190-50 50 preparations
EUR 486

ExoQuick-CG cGMP-grade Exosome Isolation reagent (50 ml)

EXOCG50A-1 50 ml
EUR 1106

50ML Protein Precipitation Kit

NAT1254 50ML
EUR 309.6

Chicken IgY precipitation reagent

85R-1010 4 ml
EUR 664.8
Description: Chicken IgY precipitation reagent for use in immunoassays

In Vivo SDS-K+ Precipitation Kit

TG1011-1 100 assays
EUR 362.4

In Vivo SDS-K+ Precipitation Kit

TG1011-2 250 assays
EUR 496.8

In Vitro SDS-K+ Precipitation Kit

TG1010-1 100 assays
EUR 550.8

In Vitro SDS-K+ Precipitation Kit

TG1010-2 250 assays
EUR 685.2

SeraMir Exosome RNA Purification kit (5ml ExoQuick and 20 exoRNA columns)

RA806A-1 20 preps
EUR 493

Kinesis TELOS PPT Protein Precipitation Plate

CHR6509 EACH
EUR 105.75

ExoQuick PLUS Exosome Purification Kit - for serum and plasma

EQPL10A-1 10 reactions
EUR 518

Kinesis TELOS PPT Protein Precipitation Columns

CHR1378 PK100
EUR 222

ExoQuick Exosome Isolation and RNA Purification kit (for Serum and Plasma)

EQ806A-1 20 preps
EUR 493

101Bio High Efficiency Protein Precipitation kit

P5W6 - Ask for price

LPA (Linear Polyacrylamide) for DNA/RNA Precipitation

10510000-1 10 mg
EUR 85.98

LPA (Linear Polyacrylamide) for DNA/RNA Precipitation

10510000-2 25 mg
EUR 140.95

ExoQuick Exosome Isolation and RNA Purification kit (for Tissue Culture Media)

EQ806TC-1 10 preps
EUR 493

ExoQuick-TC PLUS Exosome Purification Kit - for tissue culture media

EQPL10TC-1 10 reactions
EUR 518

SeraMir Exosome RNA Purification kit (10ml ExoQuick-TC and 10 exoRNA columns) 10 preps

RA806TC-1 10 preps
EUR 493

SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns)

RA800TC-1 10 reactions
EUR 595

Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome)

P101EZ - Ask for price

Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome)

P100EZ - Ask for price

Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome) sample

P101EZ--sample - Ask for price

Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome) sample

P100EZ-sample - Ask for price

ExoQuick TC 10ml

EXOTC10A-1 10 ml
EUR 341

ExoQuick TC 50ml

EXOTC50A-1 50 ml
EUR 1147

Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800TC-1 with 50ml ExoQuick-TC, RA805A-1 and RA812A-1 components)

RA822TC-1 20 profiles
EUR 1533

TMB Precipitating (High Sensitivity)

TM5125 125 ml
EUR 102

TMB Precipitating (High Sensitivity)

TM5500 500 ml
EUR 165.6

TMB Precipitating (High Sensitivity)

TM5999 1000 ml
EUR 249.6

TMB Precipitating Reagent (For Membranes)

TMP125 125 ml
EUR 96

TMB Precipitating Reagent (For Membranes)

TMP500 500 ml
EUR 159.6

TMB Precipitating Reagent (For Membranes)

TMP999 1000 ml
EUR 236.4

ExoQuick ULTRA EV Isolation Kit for Serum and Plasma

EQULTRA-20A-1 20 reactions
EUR 941

TMB Precipitating (Standard Sensitivity)

TM3125 125 ml
EUR 96

TMB Precipitating (Standard Sensitivity)

TM3500 500 ml
EUR 156

TMB Precipitating (Standard Sensitivity)

TM3999 1000 ml
EUR 235.2

Universal Protein Precipitating Reagent

BSP012 100preps
EUR 97.58

ExoQuick-TC ULTRA EV Isolation Kit for Tissue Culture Media

EQULTRA-20TC-1 20 reactions
EUR 518

Exosome binding buffer for ELISAs (20 ml)

EXOEL-BB-20 20 ml
EUR 262.8

Calcium carbonate, 99%, precipitated, Ph. Eur., BP, USP, FCC grade

GX0868-1 1
EUR 33.1

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Cell Media, Luminometric)

K1210-100 each
EUR 1161.6

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Cell media, Colorimetric)

K1205-100 each
EUR 1161.6

Wizard Precipitant Synergy - Tubes (Precipitant Synergy Protein Crystallization Screen Kit), 192 formulations, 1.7 mL volumes in tubes

M-R-1009539 192 X 1.7 ml
EUR 2064
Description: Wizard Precipitant Synergy - Tubes (Precipitant Synergy Protein Crystallization Screen Kit), 192 formulations, 1.7 mL volumes in tubes

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Urine, Colorimetric)

K1202-100 each
EUR 1161.6

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Serum, Colorimetric)

K1203-100 each
EUR 1161.6

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Plasma, Luminometric)

K1206-100 each
EUR 1161.6

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Urine, Luminometric)

K1207-100 each
EUR 1161.6

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Serum, Luminometric)

K1208-100 each
EUR 1161.6

20% SDS Solution

S0793-050 500ml
EUR 144

20% SDS Solution

S0793-100 2X500ml
EUR 199.2

*Innovative Grade US Origin Rabbit Barium Chloride Precipitate Plasma Lyophilized

IRBBACL2PPLALY500ML each
EUR 1045
Description: *Innovative Grade US Origin Rabbit Barium Chloride Precipitate Plasma Lyophilized

*Innovative Grade US Origin Rabbit Barium Chloride Precipitate Plasma Lyophilized

IRBBACL2PPLALY50ML each
EUR 150
Description: *Innovative Grade US Origin Rabbit Barium Chloride Precipitate Plasma Lyophilized

ExoQuant? Overall Exosome Capture and Quantification ELISA Assay Kit (Human Plasma, Colorimetric)

K1201-100 each
EUR 1161.6

Formalin Solution (20%)

FRL125 125 ml
EUR 75.6

Formalin Solution (20%)

FRL500 500 ml
EUR 98.4

Formalin Solution (20%)

FRL999 1000 ml
EUR 121.2

Psoriasis Exosome

P141-PS - Ask for price

Exosome Isolation kit (for serum & plasma -Exosome-RNA-NGS)

P101NGS - Ask for price

HSA Ag (20% Solution)

30-2026 100 ml Ask for price
Description: Human Serum Albumin

HSA Solution 20% (cGMP)

AK8214-0100 100mL Ask for price

100ML SDS Solution 20%

NAT1228 100ML
EUR 48

450ML SDS Solution 20%

NAT1230 450ML
EUR 118.8

SDS, 20% Liquid Solution

18-234 500ml/Unit
EUR 334

SDS, 20% Liquid Solution

18-234B 1000ml/Unit
EUR 390

Lung Cancer Exosome

P141-LG - Ask for price

Leukemia - ALL Exosome

P141-ALL - Ask for price

Leukemia - AML Exosome

P141-AML - Ask for price

Leukemia - CLL Exosome

P141-CLL - Ask for price

Leukemia - CML Exosome

P141-CML - Ask for price

Liver Cancer Exosome

P141-LV - Ask for price

Exosome FACS sorting

S107 -
EUR 2000

Gentamicin Solution, Gentamicin Solution, 50 mg/mL

CCM1123-010 10 mL
EUR 76.86

Breast Cancer Exosome

P141-BR - Ask for price

Crohn's Disease Exosome

P141-CR - Ask for price

Kidney Cancer Exosome

P141-KN - Ask for price

Psoriasis Exosome RNA

P241-PS - Ask for price

Exosome Isolation Kit

E601E001 20 tests
EUR 2500

cleaning solution 500 ml

B530 ea
EUR 36

Bladder Cancer Exosome

P141-BL - Ask for price

Thyroid Cancer Exosome

P141-TH - Ask for price

SeraMir Exosome RNA Purification Column kit (20 exoRNA columns)

RA808A-1 20 preps
EUR 344

SDS, 20%(w/v) solution

SD8119 100ml
EUR 76.7

Glycerol, 20% Sterile Solution

GR103065 100 mL
EUR 59

Prostate Cancer Exosome

P141-PR - Ask for price

Exosome Biotin labeling

S106 -
EUR 800

Timentin Solution, 100mg/mL

40110060-1 10 mL
EUR 43

Timentin Solution, 100mg/mL

40110060-2 10 mL
EUR 51.77

Timentin Solution, 100mg/mL

40110060-3 50 mL
EUR 97.01

Timentin Solution, 100mg/mL

40110060-4 100 mL
EUR 170.21

Morpheus - Precipitant Mix 4, 100mL *SHIPPED ON ICE*

M-MD2-100-84 each
EUR 312
Description: Morpheus - Precipitant Mix 4, 100mL *SHIPPED ON ICE*

Colorectal Cancer Exosome

P141-CO - Ask for price

Exosome antibody labeling

S105 -
EUR 800

Arabinose induction solution (20%)

MN850A-1 20 ml
EUR 75

Lung Cancer Exosome RNA

P241-LG - Ask for price

Exosome (Pre-isolated) RNA Extraction kit Note! This kit doesn't contain the conponents for exosome enrichment.

EIRK-01 50 reactions
EUR 3380.4
Description: A RNA Extraction kit that is ideal for total RNA (miRNA+mRNAs) extraction from pre-isolated exosomes.

Diabetes (Type 1) Exosome

P141-DB1 - Ask for price

Diabetes (Type 2) Exosome

P141-DB2 - Ask for price

Normal human exosome RNA

P240 - Ask for price

Leukemia - ALL Exosome RNA

P241-ALL - Ask for price

Leukemia - AML Exosome RNA

P241-AML - Ask for price

Leukemia - CLL Exosome RNA

P241-CLL - Ask for price

Leukemia - CML Exosome RNA

P241-CML - Ask for price

Liver Cancer Exosome RNA

P241-LV - Ask for price

Exosome-TEM-easy Kit

P130 - Ask for price

Exo-APC Exosome FACS stain

EXOFLOW810A-1 20 reactions
EUR 188

X-Gal Solution, 100mg/mL

40121144-1 5 mL
EUR 43.01

X-Gal Solution, 100mg/mL

40121144-2 10 mL
EUR 63.14

EXOCET Exosome Quantitation Kit

EXOCET96A-1 96 reactions
EUR 462

Breast Cancer Exosome RNA

P241-BR - Ask for price

Crohn's Disease Exosome RNA

P241-CR - Ask for price

Kidney Cancer Exosome RNA

P241-KN - Ask for price

Exo-FITC Exosome FACS stain

EXOFLOW800A-1 20 reactions
EUR 188

Dispase Solution, 1mg/ml

CA092-010 100ml
EUR 134.4

Luminol Solution, 1mg/mL

40120074-1 25 mL
EUR 15.1

Luminol Solution, 1mg/mL

40120074-2 100 mL
EUR 27.17

Rheumatoid Arthritis Exosome

P141-RA - Ask for price

Exosome fluorescent staining

S104 -
EUR 800

ExoQuant? Tumor-derived Exosome enrichment and Quantification Assay Kit (Biological Fluids/Cell Media, Colorimetric)

K1204-100 each
EUR 1161.6

ExoQuant? Tumor-derived Exosome enrichment and Quantification Assay Kit (Biological Fluids/Cell Media, Luminometric)

K1209-100 each
EUR 1161.6

Puromycin DiHCl Solution (10 mg/mL in 20 mM HEPES)

P025-P026-P025-5x1ML P025-5 x 1 mL
EUR 148.8

Puromycin DiHCl Solution (10 mg/mL in 20 mM HEPES)

P026-P026-20ML P026-20 mL
EUR 337.2

Bladder Cancer Exosome RNA

P241-BL - Ask for price

Thyroid Cancer Exosome RNA

P241-TH - Ask for price

20 x Synovial Fluid Solution

GR103059 100 mL
EUR 70

Nystatin Solution, 5mg/mL

41410012-1 10 mL
EUR 24.65

Pronase Solution, 10mg/mL

21730016-1 10 mL
EUR 105.48

Lyophilized Exosome Standards

ExoA375 100 μg
EUR 364.2

Lyophilized Exosome Standards

ExoHT29 100 μg
EUR 364.2

Lyophilized Exosome Standards

ExoMCF7 100 μg
EUR 364.2

Lyophilized Exosome Standards

ExoPC3 100 μg
EUR 364.2

Lyophilized Exosome Standards

ExoPLASMA 100 μg
EUR 364.2

Lyophilized Exosome Standards

ExoUrine 100 μg
EUR 364.2

Nasopharyngeal Cancer Exosome

P141-NA - Ask for price

Timentin Solution, 50mg/mL

40110059-1 20 mL
EUR 23.8

Timentin Solution, 50mg/mL

40110059-2 50 mL
EUR 33.86

Timentin Solution, 50mg/mL

40110059-3 100 mL
EUR 44.84

Glycogen Solution, 20mg/mL

11170053-1 1 mL
EUR 76.66

Leukemia - Hairy Cell Exosome

P141-HC - Ask for price

Prostate Cancer Exosome RNA

P241-PR - Ask for price

pepsine cleaning solution 1000 ml

B232 ea
EUR 118.8

Kanamycin solution (50mg/ml)

MN800A-1 20 ml
EUR 75

AR amplification CRPC were detected in most samples were analyzed using either a cell-free DNA (cfDNA) or exosome isolation kit (80%). We show that the selective isolation of exosomes enriched subset for the origin of the tumor, rather than the total analysis of exosomes plasma or plasma total nucleic acids, increased sensitivity and specificity to detect specific changes in circulation.