Optimization of microRNA Acquirement from Seminal Plasma and Identification of Diminished Seminal microRNA-34b as Indicator of Low Semen Concentration

Approximately 10-15% of couples who want to conceive suffer from subfertility, while in 30% of these cases, male factors play a role. The level of specific microRNAs in seminal plasma, including those involved in spermatogenesis, can serve as a parameter indicative of subfertility. We first optimized protocols to obtain microRNAs from seminal plasma.

Next, using a test strategy is validated in a group of men, we aim to identify microRNAs whose levels associated with semen motility and concentration. By qPCR, 742 microRNAs are profiled in normozoospermic three samples, three samples of sperm and low sperm motility (asthenozoospermia), and three with semen low concentration (oligozoospermia). MicroRNAs show a significant difference between the groups further validated in a second group consisting of 40 samples with normozoospermia (control group), 47 samples with asthenozoospermia, and 19 samples with oligozoospermia (which is 74% too low motility).

MicroRNA highest results obtained with biofluids RNA extraction kits, with the inclusion of MS2 RNA and proteinase K treatment operator for the protocol, and when 50 uL seminal plasma is used as input. Exosome RNA isolation before extraction does not lead to improved results. In the test cohort, 236 microRNAs can be detected, of which 54 microRNAs showed differences between groups. Five microRNAs analyzed in the validation group. the level of MiR-34b-5p in the control group was significantly higher compared with asthenozoospermia group (p <0.05) and compared with oligozoospermia group (p <0.001).

We optimized the acquisition of seminal plasma microRNA and microRNA levels were identified in relation to sperm concentration and motility. As the latest human and rat studies showed that miR-34 family is a marker of low concentration of semen and is very important in spermatogenesis, seminal plasma miR-34b-5p may be a suitable candidate for further study as a marker of male subfertility.

 Optimization of microRNA Acquirement from Seminal Plasma and Identification of Diminished Seminal microRNA-34b as Indicator of Low Semen Concentration
Optimization of microRNA Acquirement from Seminal Plasma and Identification of Diminished Seminal microRNA-34b as Indicator of Low Semen Concentration

When Less Is More: Special Capture and Analysis in the Plasma Tumor Exosom Liquid Biopsy Improves Sensitivity for Detection of Multiple androgen receptor Comprehensive Phenotypes in Advanced Prostate Cancer Patients

We evaluate the advantages and reliability of new protocols for enriching tumor extracellular vesicles (EV), allowing blood-based test for noninvasive parallel profiles of some of the androgen receptor (AR) gene changes. Three AR clinically relevant variants associated with response / resistance to standard-of-care treatment (AR-V7 transcripts, a point mutation AR T878A and AR gene amplification) were evaluated by digital PCR in 15 samples from patients affected by Castration-Resistant Prostate Cancer (CRPC).

Plasma is processed to obtain circulating RNA and DNA using protocols based enrichment EVS tumor through immuno-affinity and peptide-affinity compared with the generic extraction kit. Our results indicate that the immuno-affinity enrichment before RNA extraction clearly exceed the generic isolation method in the detection of the AR-V7, also allow for the differences between the respondents (R) and non-responders (NR) patients.The T878A mutation is detected, as a whole, in nine out of 15 samples, and no one approach alone is capable of expressing the mutation in all samples store, indicating that the method employed complementary.

Retrovirus Precipitation Solution

VC200 100 ml
EUR 194.04

Retrovirus Precipitation Solution

VC225 250 ml
EUR 432.6

Retrovirus Precipitation Solution

VC250 500 ml
EUR 814.8

Exosome precipitation from Plasma + Thrombin

EPStep-T 5 ml
EUR 291.5
Description: Exosome precipitation from Plasma + Thrombin - 5ml

PEG-it Virus Precipitation Solution (100 mL aliquot)

LV810A-1 100 ml
EUR 325

PEG-it Virus Precipitation Solution (250 mL aliquot)

LV825A-1 250 ml
EUR 711

Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL)

TMEXO-1 100 reactions
EUR 522

TMB for Precipitation

42R-TB100x 1000 ml
EUR 473
Description: High sensitivity TMB Substrate for Precipitation assays

TMB for Precipitation

MBS538888-1L 1L
EUR 620

TMB for Precipitation

MBS538888-5x1L 5x1L
EUR 2700

DNA Precipitation Buffer

D106 55 ml
EUR 210
Description: DNA Precipitation Buffer by Cygnus Technologies is available in Europe via Gentaur.

PEG Virus Precipitation Kit

K904-200 each
EUR 1201.2

PEG Virus Precipitation Kit

K904-50 each
EUR 451.2

PEG Virus Precipitation Kit

K2190-200 200 preparations
EUR 1270.8

PEG Virus Precipitation Kit

K2190-50 50 preparations
EUR 418
Description: High Viral Titer, No ultracentrifugation

ExoQuick Exosome RNA Column Purification kit

EQ808A-1 20 preps
EUR 344

Chicken IgY precipitation reagent

85R-1010 4 ml
EUR 638
Description: Chicken IgY precipitation reagent for use in immunoassays

Chicken IgY precipitation reagent

MBS5307794-2mL 2mL
EUR 975

Chicken IgY precipitation reagent

MBS5307794-5x2mL 5x2mL
EUR 4245

In Vivo SDS-K+ Precipitation Kit

TG1011-1 100 assays
EUR 362.4

In Vivo SDS-K+ Precipitation Kit

TG1011-2 250 assays
EUR 496.8

Nucleic Acid Precipitation Enhancer

TBS6010 2 x0.5 mL
EUR 98

ExoQuick-LP for lipoprotein pre-clear & exosome isolation

EXOLP5A-1 5 reactions
EUR 473

In Vitro SDS-K+ Precipitation Kit

TG1010-1 100 assays
EUR 550.8

In Vitro SDS-K+ Precipitation Kit

TG1010-2 250 assays
EUR 685.2

50ML Protein Precipitation Kit - 50ML

NAT1254 50ML
EUR 348.3

MinuteTM Native Protein Precipitation Kit from Saliva (20 tests)

SV-043 each
EUR 330

PEG Virus Precipitation Kit - 200 preparations

GWB-AXR384 200 preparations Ask for price

PEG Virus Precipitation Kit - 50 preparations

GWB-AXR385 50 preparations Ask for price

ExoQuick-CG cGMP-grade Exosome Isolation reagent (50 ml)

EXOCG50A-1 50 ml
EUR 1106

101Bio High Efficiency Protein Precipitation kit

P5W6 30 ml / 30 ml
EUR 129

Kinesis TELOS PPT Protein Precipitation Plate - EACH

CHR6509 EACH
EUR 118.97

SeraMir Exosome RNA Purification kit (5ml ExoQuick and 20 exoRNA columns)

RA806A-1 20 preps
EUR 493

LPA (Linear Polyacrylamide) for DNA/RNA Precipitation

10510000-1 10 mg
EUR 85.98

LPA (Linear Polyacrylamide) for DNA/RNA Precipitation

10510000-2 25 mg
EUR 140.95

Minute TM High Efficiency Protein Precipitation kit

WA-006 each
EUR 160

Kinesis TELOS PPT Protein Precipitation Columns - PK100

CHR1378 PK100
EUR 249.75

ExoQuick PLUS Exosome Purification Kit - for serum and plasma

EQPL10A-1 10 reactions
EUR 518

ExoQuick Exosome Isolation and RNA Purification kit (for Serum and Plasma)

EQ806A-1 20 preps
EUR 493

ExoQuick Exosome Isolation and RNA Purification kit (for Tissue Culture Media)

EQ806TC-1 10 preps
EUR 493

ExoQuick-TC PLUS Exosome Purification Kit - for tissue culture media

EQPL10TC-1 10 reactions
EUR 518

SeraMir Exosome RNA Purification kit (10ml ExoQuick-TC and 10 exoRNA columns) 10 preps

RA806TC-1 10 preps
EUR 493

SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns)

RA800TC-1 10 reactions
EUR 595

Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome)

P101EZ - Ask for price

Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome)

P100EZ - Ask for price

Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome) sample

P101EZ--sample - Ask for price

ExoQuick TC 10ml

EXOTC10A-1 10 ml
EUR 341

ExoQuick TC 50ml

EXOTC50A-1 50 ml
EUR 1147

Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome) sample

P100EZ-sample - Ask for price

Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800TC-1 with 50ml ExoQuick-TC, RA805A-1 and RA812A-1 components)

RA822TC-1 20 profiles
EUR 1533

TMB Precipitating (High Sensitivity)

TM5125 125 ml
EUR 28.28

TMB Precipitating (High Sensitivity)

TM5500 500 ml
EUR 70.71

TMB Precipitating (High Sensitivity)

TM5999 1000 ml
EUR 127.28

TMB Precipitating Reagent (For Membranes)

TMP125 125 ml
EUR 96

TMB Precipitating Reagent (For Membranes)

TMP500 500 ml
EUR 159.6

TMB Precipitating Reagent (For Membranes)

TMP999 1000 ml
EUR 236.4

TMB Precipitating (Standard Sensitivity)

TM3125 125 ml
EUR 24.43

TMB Precipitating (Standard Sensitivity)

TM3500 500 ml
EUR 64.28

TMB Precipitating (Standard Sensitivity)

TM3999 1000 ml
EUR 117

Universal Protein Precipitating Reagent

BSP012 100preps
EUR 97.58

ExoQuick ULTRA EV Isolation Kit for Serum and Plasma

EQULTRA-20A-1 20 reactions
EUR 941

ExoQuick-TC ULTRA EV Isolation Kit for Tissue Culture Media

EQULTRA-20TC-1 20 reactions
EUR 518

Sulfur Precipitated pure, 98%

69181 500 Gms
EUR 1.73
Description: Part B

Silicic acid, precipitated

GRM622-500G 1 unit
EUR 13.55
Description: Silicic acid, precipitated

Sulphur precipitated, Hi-AR™

RM1316-500G 1 unit
EUR 11.59
Description: Sulphur precipitated, Hi-AR™

ExoQualiTM Exosome DNA Extraction Kit (Pre-Isolated Exosomes)

CEIK-050L-30T 30T Ask for price

ExoQualiTM Exosome DNA Extraction Kit (Pre-Isolated Exosomes)

CEIK-050L-50T 50T Ask for price

ExoQualiTM Exosome RNA Extraction Kit (Pre-Isolated Exosomes)

CEIK-051L-30T 30T Ask for price

ExoQualiTM Exosome RNA Extraction Kit (Pre-Isolated Exosomes)

CEIK-051L-50T 50T Ask for price

Calcium resinate (precipitated)

C01800 25G
EUR 132.08

Magnesium stearate precipitated fine pow

GRM7219-500G 1 unit
EUR 6.93
Description: Magnesium stearate precipitated fine pow

Rabbit plasma, barium chloride precipitate

MBS136153-500mL 500mL
EUR 1360

Rabbit plasma, barium chloride precipitate

MBS136153-50mL 50mL
EUR 270

Rabbit plasma, barium chloride precipitate

MBS136153-5x500mL 5x500mL
EUR 5930

ExoQualiTM Exosome Isolation Kit (Urine)

CEIK-003L-10T 10 T Ask for price

ExoQualiTM Exosome Isolation Kit (Urine)

CEIK-003L-30T 30 T Ask for price

ExoQualiTM Exosome Isolation Kit (Serum)

CEIK-005L-10T 10 T Ask for price

ExoQualiTM Exosome Isolation Kit (Serum)

CEIK-005L-30T 30 T Ask for price

Calcium carbonate, 99%, precipitated, Ph. Eur., BP, USP, FCC grade

GX0868 1kg
EUR 30.35

Calcium carbonate, 99%, precipitated, Ph. Eur., BP, USP, FCC grade

GX0868-1 1
EUR 33.1

ExoQualiTM Exosome Isolation Kit (Plasma)

CEIK-004L-10T 10 T Ask for price

ExoQualiTM Exosome Isolation Kit (Plasma)

CEIK-004L-30T 30 T Ask for price

ExoQualiTM Exosome Chromatography Columns

ESEC-001L-5columns 5 columns Ask for price

ExoQualiTM Exosome Isolation Kit (Cell Media)

CEIK-001L-10T 10 T Ask for price

ExoQualiTM Exosome Isolation Kit (Cell Media)

CEIK-001L-30T 30 T Ask for price

ExoQualiTM Exosome Isolation Kit (All in one)

CEIK-008L-10T 10 T Ask for price

ExoQualiTM Exosome Isolation Kit (All in one)

CEIK-008L-30T 30 T Ask for price

ExoQualiTM Max Exosome Chromatography Columns

ESEC-002L-3columns 3 columns Ask for price

ExoQualiTM Exosome Isolation Kit (Plasma & Serum)

CEIK-006L-10T 10 T Ask for price

ExoQualiTM Exosome Isolation Kit (Plasma & Serum)

CEIK-006L-30T 30 T Ask for price

ExoQualiTM Mini Exosome Chromatography Columns

ESEC-003L-2columns 2 columns Ask for price

ExoQualiTM Exosome Isolation Kit (Stem Cell Media)

CEIK-002L-10T 10 T Ask for price

ExoQualiTM Exosome Isolation Kit (Stem Cell Media)

CEIK-002L-30T 30 T Ask for price

ExoQualiTM Exosome Isolation Kit (other body fluids)

CEIK-007L-10T 10 T Ask for price

ExoQualiTM Exosome Isolation Kit (other body fluids)

CEIK-007L-30T 30 T Ask for price

ExoQualiTM Exosome DNA Extraction Kit (Plasma & Serum)

CEIK-048L-30T 30T Ask for price

ExoQualiTM Exosome DNA Extraction Kit (Plasma & Serum)

CEIK-048L-50T 50T Ask for price

ExoQualiTM Exosome RNA Extraction Kit (Plasma & Serum)

CEIK-093L-30T 30T Ask for price

ExoQualiTM Exosome RNA Extraction Kit (Plasma & Serum)

CEIK-093L-50T 50T Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Serum )

CEIK-070L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Serum )

CEIK-070L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Urine )

CEIK-071L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Urine )

CEIK-071L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Plasma )

CEIK-068L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Plasma )

CEIK-068L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Saliva )

CEIK-069L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Saliva )

CEIK-069L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from serum)

CEIK-044L-30T 30 T Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from serum)

CEIK-044L-50T 50 T Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from urine)

CEIK-046L-10T 10 T Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from urine)

CEIK-046L-24T 24 T Ask for price

ExoQuali™ Lyophilized Exosome Standards ( MM1 cell line )

CEIK-064L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( MM1 cell line )

CEIK-064L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( PC3 cell line )

CEIK-065L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( PC3 cell line )

CEIK-065L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from plasma)

CEIK-045L-30T 30 T Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from plasma)

CEIK-045L-50T 50 T Ask for price

ExoQuali™ Lyophilized Exosome Standards ( A549 cell line )

CEIK-054L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( A549 cell line )

CEIK-054L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQualiTM Total Exosome Capture & Quantification Kit (Serum)

CEIK-107L-96wells 96 wells Ask for price

ExoQualiTM Total Exosome Capture & Quantification Kit (Urine)

CEIK-110L-96wells 96 wells Ask for price

ExoQuali™ Lyophilized Exosome Standards ( COLO1 cell line )

CEIK-058L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( COLO1 cell line )

CEIK-058L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQualiTM Total Exosome Capture & Quantification Kit (Plasma)

CEIK-108L-96wells 96 wells Ask for price

ExoQuali™ Lyophilized Exosome Standards ( U87 MG cell line )

CEIK-067L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( U87 MG cell line )

CEIK-067L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

Exosome binding buffer for ELISAs (20 ml)

EXOEL-BB-20 20 ml
EUR 262.8

ExoQuali™ Lyophilized Exosome Standards ( B16F10 cell line )

CEIK-055L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( B16F10 cell line )

CEIK-055L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( BLCL21 cell line )

CEIK-056L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( BLCL21 cell line )

CEIK-056L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( HCT116 cell line )

CEIK-060L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( HCT116 cell line )

CEIK-060L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( BPH-1 cell line )

CEIK-057L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( BPH-1 cell line )

CEIK-057L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( K-562 cell line )

CEIK-062L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( K-562 cell line )

CEIK-062L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQualiTM Overall Exosome Isolation Immunoplates (from Serum)

CEIK-023L-96wells 96 wells Ask for price

ExoQualiTM Overall Exosome Isolation Immunoplates (from Saliva)

CEIK-026L-96wells 96 wells Ask for price

ExoQualiTM Overall Exosome Isolation Immunoplates (from Plasma)

CEIK-095L-96wells 96 wells Ask for price

ExoQualiTM Overall Exosome Isolation Immunoplates (from Urine)

CEIK-096L-96wells 96 wells Ask for price

ExoQualiTM Exosome DNA Extraction Kit (Urine & Cell Supernatant)

CEIK-049L-10T 10T Ask for price

ExoQualiTM Exosome DNA Extraction Kit (Urine & Cell Supernatant)

CEIK-049L-20T 20T Ask for price

ExoQualiTM Exosome RNA Extraction Kit (Urine & Cell Supernatant)

CEIK-094L-10T 10T Ask for price

ExoQualiTM Exosome RNA Extraction Kit (Urine & Cell Supernatant)

CEIK-094L-20T 20T Ask for price

*Innovative Grade US Origin Rabbit Barium Chloride Precipitate Plasma Lyophilized

IRBBACL2PPLALY500ML each
EUR 1045
Description: *Innovative Grade US Origin Rabbit Barium Chloride Precipitate Plasma Lyophilized

*Innovative Grade US Origin Rabbit Barium Chloride Precipitate Plasma Lyophilized

IRBBACL2PPLALY50ML each
EUR 150
Description: *Innovative Grade US Origin Rabbit Barium Chloride Precipitate Plasma Lyophilized

ExoQuali™ Lyophilized Exosome Standards ( SK-N-SH cell line )

CEIK-066L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( SK-N-SH cell line )

CEIK-066L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQualiTM Overall Exosome Isolation Immunoplates (from Cell Media)

CEIK-029L-96wells 96 wells Ask for price

Wizard Precipitant Synergy - Tubes (Precipitant Synergy Protein Crystallization Screen Kit), 192 formulations, 1.7 mL volumes in tubes

M-R-1009539 192 X 1.7 ml
EUR 2064
Description: Wizard Precipitant Synergy - Tubes (Precipitant Synergy Protein Crystallization Screen Kit), 192 formulations, 1.7 mL volumes in tubes

ExoQualiTM Overall Exosome Isolation Reagent (from other body fluids)

CEIK-047L-10T 10 T Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from other body fluids)

CEIK-047L-24T 24 T Ask for price

RNase A Solution (20 mg/ml)

DS0003-15ML 1 unit
EUR 211.62
Description: RNase A Solution (20 mg/ml)

RNase A Solution (20 mg/ml)

DS0003-2ML 1 unit
EUR 45.01
Description: RNase A Solution (20 mg/ml)

RNase A Solution (20 mg/ml)

DS0003-5ML 1 unit
EUR 88.21
Description: RNase A Solution (20 mg/ml)

AR amplification CRPC were detected in most samples were analyzed using either a cell-free DNA (cfDNA) or exosome isolation kit (80%). We show that the selective isolation of exosomes enriched subset for the origin of the tumor, rather than the total analysis of exosomes plasma or plasma total nucleic acids, increased sensitivity and specificity to detect specific changes in circulation.