Approximately 10-15% of couples who want to conceive suffer from subfertility, while in 30% of these cases, male factors play a role. The level of specific microRNAs in seminal plasma, including those involved in spermatogenesis, can serve as a parameter indicative of subfertility. We first optimized protocols to obtain microRNAs from seminal plasma.
Next, using a test strategy is validated in a group of men, we aim to identify microRNAs whose levels associated with semen motility and concentration. By qPCR, 742 microRNAs are profiled in normozoospermic three samples, three samples of sperm and low sperm motility (asthenozoospermia), and three with semen low concentration (oligozoospermia). MicroRNAs show a significant difference between the groups further validated in a second group consisting of 40 samples with normozoospermia (control group), 47 samples with asthenozoospermia, and 19 samples with oligozoospermia (which is 74% too low motility).
MicroRNA highest results obtained with biofluids RNA extraction kits, with the inclusion of MS2 RNA and proteinase K treatment operator for the protocol, and when 50 uL seminal plasma is used as input. Exosome RNA isolation before extraction does not lead to improved results. In the test cohort, 236 microRNAs can be detected, of which 54 microRNAs showed differences between groups. Five microRNAs analyzed in the validation group. the level of MiR-34b-5p in the control group was significantly higher compared with asthenozoospermia group (p <0.05) and compared with oligozoospermia group (p <0.001).
We optimized the acquisition of seminal plasma microRNA and microRNA levels were identified in relation to sperm concentration and motility. As the latest human and rat studies showed that miR-34 family is a marker of low concentration of semen and is very important in spermatogenesis, seminal plasma miR-34b-5p may be a suitable candidate for further study as a marker of male subfertility.
Optimization of microRNA Acquirement from Seminal Plasma and Identification of Diminished Seminal microRNA-34b as Indicator of Low Semen Concentration
When Less Is More: Special Capture and Analysis in the Plasma Tumor Exosom Liquid Biopsy Improves Sensitivity for Detection of Multiple androgen receptor Comprehensive Phenotypes in Advanced Prostate Cancer Patients
We evaluate the advantages and reliability of new protocols for enriching tumor extracellular vesicles (EV), allowing blood-based test for noninvasive parallel profiles of some of the androgen receptor (AR) gene changes. Three AR clinically relevant variants associated with response / resistance to standard-of-care treatment (AR-V7 transcripts, a point mutation AR T878A and AR gene amplification) were evaluated by digital PCR in 15 samples from patients affected by Castration-Resistant Prostate Cancer (CRPC).
Plasma is processed to obtain circulating RNA and DNA using protocols based enrichment EVS tumor through immuno-affinity and peptide-affinity compared with the generic extraction kit. Our results indicate that the immuno-affinity enrichment before RNA extraction clearly exceed the generic isolation method in the detection of the AR-V7, also allow for the differences between the respondents (R) and non-responders (NR) patients.The T878A mutation is detected, as a whole, in nine out of 15 samples, and no one approach alone is capable of expressing the mutation in all samples store, indicating that the method employed complementary.
Retrovirus Precipitation Solution
VC200
ALSTEM
100 ml
EUR 194.04
Retrovirus Precipitation Solution
VC225
ALSTEM
250 ml
EUR 432.6
Retrovirus Precipitation Solution
VC250
ALSTEM
500 ml
EUR 814.8
Exosome precipitation from Plasma + Thrombin
EPStep-T
ImmunoStep
5 ml
EUR 291.5
Description: Exosome precipitation from Plasma + Thrombin - 5ml
PEG-it Virus Precipitation Solution (100 mL aliquot)
LV810A-1
SBI
100 ml
EUR 325
PEG-it Virus Precipitation Solution (250 mL aliquot)
LV825A-1
SBI
250 ml
EUR 711
Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL)
TMEXO-1
SBI
100 reactions
EUR 522
TMB for Precipitation
42R-TB100x
Fitzgerald
1000 ml
EUR 473
Description: High sensitivity TMB Substrate for Precipitation assays
TMB for Precipitation
MBS538888-1L
MyBiosource
1L
EUR 620
TMB for Precipitation
MBS538888-5x1L
MyBiosource
5x1L
EUR 2700
DNA Precipitation Buffer
D106
Cygnus Technologies
55 ml
EUR 210
Description: DNA Precipitation Buffer by Cygnus Technologies is available in Europe via Gentaur.
PEG Virus Precipitation Kit
K904-200
Biovision
each
EUR 1201.2
PEG Virus Precipitation Kit
K904-50
Biovision
each
EUR 451.2
PEG Virus Precipitation Kit
K2190-200
ApexBio
200 preparations
EUR 1270.8
PEG Virus Precipitation Kit
K2190-50
ApexBio
50 preparations
EUR 418
Description: High Viral Titer, No ultracentrifugation
ExoQuick Exosome RNA Column Purification kit
EQ808A-1
SBI
20 preps
EUR 344
Chicken IgY precipitation reagent
85R-1010
Fitzgerald
4 ml
EUR 638
Description: Chicken IgY precipitation reagent for use in immunoassays
Chicken IgY precipitation reagent
MBS5307794-2mL
MyBiosource
2mL
EUR 975
Chicken IgY precipitation reagent
MBS5307794-5x2mL
MyBiosource
5x2mL
EUR 4245
In Vivo SDS-K+ Precipitation Kit
TG1011-1
TopoGen
100 assays
EUR 362.4
In Vivo SDS-K+ Precipitation Kit
TG1011-2
TopoGen
250 assays
EUR 496.8
Nucleic Acid Precipitation Enhancer
TBS6010
Tribioscience
2 x0.5 mL
EUR 98
ExoQuick-LP for lipoprotein pre-clear & exosome isolation
EXOLP5A-1
SBI
5 reactions
EUR 473
In Vitro SDS-K+ Precipitation Kit
TG1010-1
TopoGen
100 assays
EUR 550.8
In Vitro SDS-K+ Precipitation Kit
TG1010-2
TopoGen
250 assays
EUR 685.2
MinuteTM Native Protein Precipitation Kit from Saliva (20 tests)
SV-043
Inventbiotech
each
EUR 330
PEG Virus Precipitation Kit - 200 preparations
GWB-AXR384
GenWay Biotech
200 preparations
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PEG Virus Precipitation Kit - 50 preparations
GWB-AXR385
GenWay Biotech
50 preparations
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ExoQuick-CG cGMP-grade Exosome Isolation reagent (50 ml)
EXOCG50A-1
SBI
50 ml
EUR 1106
101Bio High Efficiency Protein Precipitation kit
P5W6
101Bio
30 ml / 30 ml
EUR 129
SeraMir Exosome RNA Purification kit (5ml ExoQuick and 20 exoRNA columns)
RA806A-1
SBI
20 preps
EUR 493
LPA (Linear Polyacrylamide) for DNA/RNA Precipitation
10510000-1
Glycomatrix
10 mg
EUR 85.98
LPA (Linear Polyacrylamide) for DNA/RNA Precipitation
10510000-2
Glycomatrix
25 mg
EUR 140.95
Minute TM High Efficiency Protein Precipitation kit
WA-006
Inventbiotech
each
EUR 160
ExoQuick PLUS Exosome Purification Kit - for serum and plasma
EQPL10A-1
SBI
10 reactions
EUR 518
ExoQuick Exosome Isolation and RNA Purification kit (for Serum and Plasma)
EQ806A-1
SBI
20 preps
EUR 493
ExoQuick Exosome Isolation and RNA Purification kit (for Tissue Culture Media)
EQ806TC-1
SBI
10 preps
EUR 493
ExoQuick-TC PLUS Exosome Purification Kit - for tissue culture media
EQPL10TC-1
SBI
10 reactions
EUR 518
SeraMir Exosome RNA Purification kit (10ml ExoQuick-TC and 10 exoRNA columns) 10 preps
RA806TC-1
SBI
10 preps
EUR 493
SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns)
RA800TC-1
SBI
10 reactions
EUR 595
Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome)
P101EZ
101Bio
-
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Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome)
P100EZ
101Bio
-
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Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome) sample
P101EZ--sample
101Bio
-
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ExoQuick TC 10ml
EXOTC10A-1
SBI
10 ml
EUR 341
ExoQuick TC 50ml
EXOTC50A-1
SBI
50 ml
EUR 1147
Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome) sample
P100EZ-sample
101Bio
-
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Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800TC-1 with 50ml ExoQuick-TC, RA805A-1 and RA812A-1 components)
RA822TC-1
SBI
20 profiles
EUR 1533
Universal Protein Precipitating Reagent
BSP012
Bio Basic
100preps
EUR 97.58
ExoQuick ULTRA EV Isolation Kit for Serum and Plasma
EQULTRA-20A-1
SBI
20 reactions
EUR 941
ExoQuick-TC ULTRA EV Isolation Kit for Tissue Culture Media
EQULTRA-20TC-1
SBI
20 reactions
EUR 518
Sulfur Precipitated pure, 98%
69181
Sisco Laboratories
500 Gms
EUR 1.73
Description: Part B
Silicic acid, precipitated
GRM622-500G
EWC Diagnostics
1 unit
EUR 13.55
Description: Silicic acid, precipitated
Sulphur precipitated, Hi-AR™
RM1316-500G
EWC Diagnostics
1 unit
EUR 11.59
Description: Sulphur precipitated, Hi-AR™
ExoQualiTM Exosome DNA Extraction Kit (Pre-Isolated Exosomes)
CEIK-050L-30T
Creative Bioarray
30T
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ExoQualiTM Exosome DNA Extraction Kit (Pre-Isolated Exosomes)
CEIK-050L-50T
Creative Bioarray
50T
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ExoQualiTM Exosome RNA Extraction Kit (Pre-Isolated Exosomes)
CEIK-051L-30T
Creative Bioarray
30T
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ExoQualiTM Exosome RNA Extraction Kit (Pre-Isolated Exosomes)
CEIK-051L-50T
Creative Bioarray
50T
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Magnesium stearate precipitated fine pow
GRM7219-500G
EWC Diagnostics
1 unit
EUR 6.93
Description: Magnesium stearate precipitated fine pow
Rabbit plasma, barium chloride precipitate
MBS136153-500mL
MyBiosource
500mL
EUR 1360
Rabbit plasma, barium chloride precipitate
MBS136153-50mL
MyBiosource
50mL
EUR 270
Rabbit plasma, barium chloride precipitate
MBS136153-5x500mL
MyBiosource
5x500mL
EUR 5930
ExoQualiTM Exosome Isolation Kit (Urine)
CEIK-003L-10T
Creative Bioarray
10 T
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ExoQualiTM Exosome Isolation Kit (Urine)
CEIK-003L-30T
Creative Bioarray
30 T
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ExoQualiTM Exosome Isolation Kit (Serum)
CEIK-005L-10T
Creative Bioarray
10 T
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ExoQualiTM Exosome Isolation Kit (Serum)
CEIK-005L-30T
Creative Bioarray
30 T
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Calcium carbonate, 99%, precipitated, Ph. Eur., BP, USP, FCC grade
GX0868
Glentham Life Sciences
1kg
EUR 30.35
ExoQualiTM Exosome Isolation Kit (Plasma)
CEIK-004L-10T
Creative Bioarray
10 T
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ExoQualiTM Exosome Isolation Kit (Plasma)
CEIK-004L-30T
Creative Bioarray
30 T
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ExoQualiTM Exosome Chromatography Columns
ESEC-001L-5columns
Creative Bioarray
5 columns
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ExoQualiTM Exosome Isolation Kit (Cell Media)
CEIK-001L-10T
Creative Bioarray
10 T
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ExoQualiTM Exosome Isolation Kit (Cell Media)
CEIK-001L-30T
Creative Bioarray
30 T
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ExoQualiTM Exosome Isolation Kit (All in one)
CEIK-008L-10T
Creative Bioarray
10 T
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ExoQualiTM Exosome Isolation Kit (All in one)
CEIK-008L-30T
Creative Bioarray
30 T
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ExoQualiTM Max Exosome Chromatography Columns
ESEC-002L-3columns
Creative Bioarray
3 columns
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ExoQualiTM Exosome Isolation Kit (Plasma & Serum)
CEIK-006L-10T
Creative Bioarray
10 T
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ExoQualiTM Exosome Isolation Kit (Plasma & Serum)
CEIK-006L-30T
Creative Bioarray
30 T
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ExoQualiTM Mini Exosome Chromatography Columns
ESEC-003L-2columns
Creative Bioarray
2 columns
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ExoQualiTM Exosome Isolation Kit (Stem Cell Media)
CEIK-002L-10T
Creative Bioarray
10 T
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ExoQualiTM Exosome Isolation Kit (Stem Cell Media)
CEIK-002L-30T
Creative Bioarray
30 T
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ExoQualiTM Exosome Isolation Kit (other body fluids)
CEIK-007L-10T
Creative Bioarray
10 T
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ExoQualiTM Exosome Isolation Kit (other body fluids)
CEIK-007L-30T
Creative Bioarray
30 T
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ExoQualiTM Exosome DNA Extraction Kit (Plasma & Serum)
CEIK-048L-30T
Creative Bioarray
30T
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ExoQualiTM Exosome DNA Extraction Kit (Plasma & Serum)
CEIK-048L-50T
Creative Bioarray
50T
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ExoQualiTM Exosome RNA Extraction Kit (Plasma & Serum)
CEIK-093L-30T
Creative Bioarray
30T
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ExoQualiTM Exosome RNA Extraction Kit (Plasma & Serum)
CEIK-093L-50T
Creative Bioarray
50T
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ExoQuali™ Lyophilized Exosome Standards ( Human Serum )
CEIK-070L-2vials1x1010particlespervial
Creative Bioarray
2 vials (> 1x10^10 particles per vial)
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ExoQuali™ Lyophilized Exosome Standards ( Human Serum )
CEIK-070L-5vials1x1010particlespervial
Creative Bioarray
5 vials (> 1x10^10 particles per vial)
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ExoQuali™ Lyophilized Exosome Standards ( Human Urine )
CEIK-071L-2vials1x1010particlespervial
Creative Bioarray
2 vials (> 1x10^10 particles per vial)
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ExoQuali™ Lyophilized Exosome Standards ( Human Urine )
CEIK-071L-5vials1x1010particlespervial
Creative Bioarray
5 vials (> 1x10^10 particles per vial)
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ExoQuali™ Lyophilized Exosome Standards ( Human Plasma )
CEIK-068L-2vials1x1010particlespervial
Creative Bioarray
2 vials (> 1x10^10 particles per vial)
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ExoQuali™ Lyophilized Exosome Standards ( Human Plasma )
CEIK-068L-5vials1x1010particlespervial
Creative Bioarray
5 vials (> 1x10^10 particles per vial)
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ExoQuali™ Lyophilized Exosome Standards ( Human Saliva )
CEIK-069L-2vials1x1010particlespervial
Creative Bioarray
2 vials (> 1x10^10 particles per vial)
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ExoQuali™ Lyophilized Exosome Standards ( Human Saliva )
CEIK-069L-5vials1x1010particlespervial
Creative Bioarray
5 vials (> 1x10^10 particles per vial)
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ExoQualiTM Overall Exosome Isolation Reagent (from serum)
CEIK-044L-30T
Creative Bioarray
30 T
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ExoQualiTM Overall Exosome Isolation Reagent (from serum)
CEIK-044L-50T
Creative Bioarray
50 T
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ExoQualiTM Overall Exosome Isolation Reagent (from urine)
CEIK-046L-10T
Creative Bioarray
10 T
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ExoQualiTM Overall Exosome Isolation Reagent (from urine)
CEIK-046L-24T
Creative Bioarray
24 T
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ExoQuali™ Lyophilized Exosome Standards ( MM1 cell line )
CEIK-064L-2vials1x1010particlespervial
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2 vials (> 1x10^10 particles per vial)
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ExoQuali™ Lyophilized Exosome Standards ( MM1 cell line )
CEIK-064L-5vials1x1010particlespervial
Creative Bioarray
5 vials (> 1x10^10 particles per vial)
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ExoQuali™ Lyophilized Exosome Standards ( PC3 cell line )
CEIK-065L-2vials1x1010particlespervial
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2 vials (> 1x10^10 particles per vial)
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ExoQuali™ Lyophilized Exosome Standards ( PC3 cell line )
CEIK-065L-5vials1x1010particlespervial
Creative Bioarray
5 vials (> 1x10^10 particles per vial)
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ExoQualiTM Overall Exosome Isolation Reagent (from plasma)
CEIK-045L-30T
Creative Bioarray
30 T
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ExoQualiTM Overall Exosome Isolation Reagent (from plasma)
CEIK-045L-50T
Creative Bioarray
50 T
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ExoQuali™ Lyophilized Exosome Standards ( A549 cell line )
CEIK-054L-2vials1x1010particlespervial
Creative Bioarray
2 vials (> 1x10^10 particles per vial)
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ExoQuali™ Lyophilized Exosome Standards ( A549 cell line )
CEIK-054L-5vials1x1010particlespervial
Creative Bioarray
5 vials (> 1x10^10 particles per vial)
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ExoQualiTM Total Exosome Capture & Quantification Kit (Serum)
CEIK-107L-96wells
Creative Bioarray
96 wells
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ExoQualiTM Total Exosome Capture & Quantification Kit (Urine)
CEIK-110L-96wells
Creative Bioarray
96 wells
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ExoQuali™ Lyophilized Exosome Standards ( COLO1 cell line )
CEIK-058L-2vials1x1010particlespervial
Creative Bioarray
2 vials (> 1x10^10 particles per vial)
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ExoQuali™ Lyophilized Exosome Standards ( COLO1 cell line )
CEIK-058L-5vials1x1010particlespervial
Creative Bioarray
5 vials (> 1x10^10 particles per vial)
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ExoQualiTM Total Exosome Capture & Quantification Kit (Plasma)
CEIK-108L-96wells
Creative Bioarray
96 wells
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ExoQuali™ Lyophilized Exosome Standards ( U87 MG cell line )
CEIK-067L-2vials1x1010particlespervial
Creative Bioarray
2 vials (> 1x10^10 particles per vial)
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ExoQuali™ Lyophilized Exosome Standards ( U87 MG cell line )
CEIK-067L-5vials1x1010particlespervial
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5 vials (> 1x10^10 particles per vial)
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Exosome binding buffer for ELISAs (20 ml)
EXOEL-BB-20
SBI
20 ml
EUR 262.8
ExoQuali™ Lyophilized Exosome Standards ( B16F10 cell line )
CEIK-055L-2vials1x1010particlespervial
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2 vials (> 1x10^10 particles per vial)
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ExoQuali™ Lyophilized Exosome Standards ( B16F10 cell line )
CEIK-055L-5vials1x1010particlespervial
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ExoQuali™ Lyophilized Exosome Standards ( BLCL21 cell line )
CEIK-056L-2vials1x1010particlespervial
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ExoQuali™ Lyophilized Exosome Standards ( BLCL21 cell line )
CEIK-056L-5vials1x1010particlespervial
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ExoQuali™ Lyophilized Exosome Standards ( HCT116 cell line )
CEIK-060L-2vials1x1010particlespervial
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ExoQuali™ Lyophilized Exosome Standards ( HCT116 cell line )
CEIK-060L-5vials1x1010particlespervial
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ExoQuali™ Lyophilized Exosome Standards ( BPH-1 cell line )
CEIK-057L-2vials1x1010particlespervial
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ExoQuali™ Lyophilized Exosome Standards ( BPH-1 cell line )
CEIK-057L-5vials1x1010particlespervial
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ExoQuali™ Lyophilized Exosome Standards ( K-562 cell line )
CEIK-062L-2vials1x1010particlespervial
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ExoQuali™ Lyophilized Exosome Standards ( K-562 cell line )
CEIK-062L-5vials1x1010particlespervial
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ExoQualiTM Overall Exosome Isolation Immunoplates (from Serum)
CEIK-023L-96wells
Creative Bioarray
96 wells
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ExoQualiTM Overall Exosome Isolation Immunoplates (from Saliva)
CEIK-026L-96wells
Creative Bioarray
96 wells
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ExoQualiTM Overall Exosome Isolation Immunoplates (from Plasma)
CEIK-095L-96wells
Creative Bioarray
96 wells
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ExoQualiTM Overall Exosome Isolation Immunoplates (from Urine)
CEIK-096L-96wells
Creative Bioarray
96 wells
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ExoQualiTM Exosome DNA Extraction Kit (Urine & Cell Supernatant)
CEIK-049L-10T
Creative Bioarray
10T
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ExoQualiTM Exosome DNA Extraction Kit (Urine & Cell Supernatant)
CEIK-049L-20T
Creative Bioarray
20T
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ExoQualiTM Exosome RNA Extraction Kit (Urine & Cell Supernatant)
CEIK-094L-10T
Creative Bioarray
10T
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ExoQualiTM Exosome RNA Extraction Kit (Urine & Cell Supernatant)
CEIK-094L-20T
Creative Bioarray
20T
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*Innovative Grade US Origin Rabbit Barium Chloride Precipitate Plasma Lyophilized
IRBBACL2PPLALY500ML
Innovative research
each
EUR 1045
Description: *Innovative Grade US Origin Rabbit Barium Chloride Precipitate Plasma Lyophilized
*Innovative Grade US Origin Rabbit Barium Chloride Precipitate Plasma Lyophilized
IRBBACL2PPLALY50ML
Innovative research
each
EUR 150
Description: *Innovative Grade US Origin Rabbit Barium Chloride Precipitate Plasma Lyophilized
ExoQuali™ Lyophilized Exosome Standards ( SK-N-SH cell line )
CEIK-066L-2vials1x1010particlespervial
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2 vials (> 1x10^10 particles per vial)
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ExoQuali™ Lyophilized Exosome Standards ( SK-N-SH cell line )
CEIK-066L-5vials1x1010particlespervial
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5 vials (> 1x10^10 particles per vial)
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ExoQualiTM Overall Exosome Isolation Immunoplates (from Cell Media)
CEIK-029L-96wells
Creative Bioarray
96 wells
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Wizard Precipitant Synergy - Tubes (Precipitant Synergy Protein Crystallization Screen Kit), 192 formulations, 1.7 mL volumes in tubes
M-R-1009539
MiTeGen
192 X 1.7 ml
EUR 2064
Description: Wizard Precipitant Synergy - Tubes (Precipitant Synergy Protein Crystallization Screen Kit), 192 formulations, 1.7 mL volumes in tubes
ExoQualiTM Overall Exosome Isolation Reagent (from other body fluids)
CEIK-047L-10T
Creative Bioarray
10 T
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ExoQualiTM Overall Exosome Isolation Reagent (from other body fluids)
CEIK-047L-24T
Creative Bioarray
24 T
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RNase A Solution (20 mg/ml)
DS0003-15ML
EWC Diagnostics
1 unit
EUR 211.62
Description: RNase A Solution (20 mg/ml)
RNase A Solution (20 mg/ml)
DS0003-2ML
EWC Diagnostics
1 unit
EUR 45.01
Description: RNase A Solution (20 mg/ml)
RNase A Solution (20 mg/ml)
DS0003-5ML
EWC Diagnostics
1 unit
EUR 88.21
Description: RNase A Solution (20 mg/ml)
AR amplification CRPC were detected in most samples were analyzed using either a cell-free DNA (cfDNA) or exosome isolation kit (80%). We show that the selective isolation of exosomes enriched subset for the origin of the tumor, rather than the total analysis of exosomes plasma or plasma total nucleic acids, increased sensitivity and specificity to detect specific changes in circulation.