Exosomes is 50- 150-nm-diameter extracellular vesicles secreted by all mammalian cell except mature red blood cells and contribute to a variety of physiological and pathological functions in the body. Many methods have been used to isolate and analyze exosomes, resulting in inconsistencies in the trial and raise the question of how to compare the results obtained using different approaches. Questions have also been raised about the purity of the preparations relating to the size and type of lipoprotein vesicles and presence.

Thus, researchers often find it challenging to identify exosome isolation protocol is optimal for their experimental needs. Our laboratory has compared ultracentrifugation and commercial precipitation- and column-based exosome isolation kit for exosome preparations. Here, we present a protocol for exosome isolation using the two most commonly used methods, ultracentrifugation and precipitation, followed by downstream analysis. We used NanoSight nanoparticle tracking analysis and flow cytometry (Cytek®) to determine the concentration and size of the exosome.

Imaging flow cytometry can be used for both exosomes size and surface markers immunophenotype in exosomes (ImageStream®). High performance liquid -Kromatografi followed by nano-flow liquid chromatography mass spectrometry (LCMS) of exosome fractions can be used to determine the presence of lipoproteins, the LCMS is able to provide a proteomic profile of exosome preparations. We found that the method of precipitation is six times faster and resulted in ~ 2.5-fold higher concentration of exosomes per milliliter compared with ultracentrifugation. Both methods produce extracellular vesicles in various sizes of exosomes, and both preparations, including apoproteins. © 2020 Wiley Periodicals LLC.

Basic Protocol 1: Pre-analytic fluid collection and processing base of Protocol 2: Exosome isolation by ultracentrifugation Alternative Protocol 1: Exosome isolation by precipitation basis of Protocol 3: Analysis of exosomes by NanoSight analysis tracking of nanoparticles Alternative Protocol 2: Analysis of exosomes by flow cytometry and imaging flow Basic cytometry Protocol 4: Downstream analysis of exosomes using high-performance liquid chromatography Basic Protocol 5: Downstream analysis of exosome proteome using nano-flow liquid chromatography-mass spectrometry.

Small insulation extracellular vesicles From Human Serum Using a combination of ultracentrifugation With Polymer-Based Deposition.

Exosome separation of lipoproteins validated by western blotting with several markers of exosomes and lipoproteins, followed by proteomic analysis using a nanoflow ultrahigh-performance liquid chromatography-electrospray ionization-tandem mass spectrometry. UC requires a relatively large amount of serum sample (at least 2 mL) but is more efficient in eliminating lipoproteins. UF method with centrifugal concentrators (300 kDa) were found to be more effective in taking serum exosomes with a low volume (50 uL). Overall, this study demonstrates the application of programming field FIAF4 for isolation / purification of exosomes from serum proteins and lipoproteins.

Methods to reproducibly isolate and enrich the small extracellular vesicles (EV) of blood is very important for clinical utilization of small EVs in cancer patients. We combine ultracentrifugation (UC) with rainfall based polymer (ExoQuick [EQ] or Total Exosome Isolation [TEI] kit) to isolate the small EVs (diameter, 30-150 nm) from the serum of breast cancer patients.

Retrovirus Precipitation Solution
VC200	ALSTEM	100 ml	EUR 194.04

Retrovirus Precipitation Solution
VC225	ALSTEM	250 ml	EUR 432.6

Retrovirus Precipitation Solution
VC250	ALSTEM	500 ml	EUR 814.8

Exosome precipitation from Plasma + Thrombin
EPStep-T	ImmunoStep	5 ml	EUR 291.5
Description: Exosome precipitation from Plasma + Thrombin - 5ml

PEG-it Virus Precipitation Solution (100 mL aliquot)
LV810A-1	SBI	100 ml	EUR 325

PEG-it Virus Precipitation Solution (250 mL aliquot)
LV825A-1	SBI	250 ml	EUR 711

Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL)
TMEXO-1	SBI	100 reactions	EUR 522

TMB for Precipitation
42R-TB100x	Fitzgerald	1000 ml	EUR 473
Description: High sensitivity TMB Substrate for Precipitation assays

TMB for Precipitation
MBS538888-1L	MyBiosource	1L	EUR 620

TMB for Precipitation
MBS538888-5x1L	MyBiosource	5x1L	EUR 2700

DNA Precipitation Buffer
D106	Cygnus Technologies	55 ml	EUR 210
Description: DNA Precipitation Buffer by Cygnus Technologies is available in Europe via Gentaur.

PEG Virus Precipitation Kit
K904-200	Biovision	each	EUR 1201.2

PEG Virus Precipitation Kit
K904-50	Biovision	each	EUR 451.2

PEG Virus Precipitation Kit
K2190-200	ApexBio	200 preparations	EUR 1270.8

PEG Virus Precipitation Kit
K2190-50	ApexBio	50 preparations	EUR 418
Description: High Viral Titer, No ultracentrifugation

ExoQuick Exosome RNA Column Purification kit
EQ808A-1	SBI	20 preps	EUR 344

Chicken IgY precipitation reagent
85R-1010	Fitzgerald	4 ml	EUR 638
Description: Chicken IgY precipitation reagent for use in immunoassays

Chicken IgY precipitation reagent
MBS5307794-2mL	MyBiosource	2mL	EUR 975

Chicken IgY precipitation reagent
MBS5307794-5x2mL	MyBiosource	5x2mL	EUR 4245

In Vivo SDS-K+ Precipitation Kit
TG1011-1	TopoGen	100 assays	EUR 362.4

In Vivo SDS-K+ Precipitation Kit
TG1011-2	TopoGen	250 assays	EUR 496.8

Nucleic Acid Precipitation Enhancer
TBS6010	Tribioscience	2 x0.5 mL	EUR 98

ExoQuick-LP for lipoprotein pre-clear & exosome isolation
EXOLP5A-1	SBI	5 reactions	EUR 473

In Vitro SDS-K+ Precipitation Kit
TG1010-1	TopoGen	100 assays	EUR 550.8

In Vitro SDS-K+ Precipitation Kit
TG1010-2	TopoGen	250 assays	EUR 685.2

50ML Protein Precipitation Kit - 50ML
NAT1254	Scientific Laboratory Supplies	50ML	EUR 348.3

PEG Virus Precipitation Kit - 200 preparations
GWB-AXR384	GenWay Biotech	200 preparations	Ask for price

PEG Virus Precipitation Kit - 50 preparations
GWB-AXR385	GenWay Biotech	50 preparations	Ask for price

ExoQuick-CG cGMP-grade Exosome Isolation reagent (50 ml)
EXOCG50A-1	SBI	50 ml	EUR 1106

101Bio High Efficiency Protein Precipitation kit
P5W6	101Bio	30 ml / 30 ml	EUR 129

Kinesis TELOS PPT Protein Precipitation Plate - EACH
CHR6509	Scientific Laboratory Supplies	EACH	EUR 118.97

LPA (Linear Polyacrylamide) for DNA/RNA Precipitation
10510000-1	Glycomatrix	10 mg	EUR 85.98

LPA (Linear Polyacrylamide) for DNA/RNA Precipitation
10510000-2	Glycomatrix	25 mg	EUR 140.95

Minute TM High Efficiency Protein Precipitation kit
WA-006	Inventbiotech	each	EUR 160

Kinesis TELOS PPT Protein Precipitation Columns - PK100
CHR1378	Scientific Laboratory Supplies	PK100	EUR 249.75

ExoQuick PLUS Exosome Purification Kit - for serum and plasma
EQPL10A-1	SBI	10 reactions	EUR 518

MinuteTM Native Protein Precipitation Kit from Saliva (20 tests)
SV-043	Inventbiotech	each	EUR 330

ExoQuick Exosome Isolation and RNA Purification kit (for Serum and Plasma)
EQ806A-1	SBI	20 preps	EUR 493

ExoQuick Exosome Isolation and RNA Purification kit (for Tissue Culture Media)
EQ806TC-1	SBI	10 preps	EUR 493

ExoQuick-TC PLUS Exosome Purification Kit - for tissue culture media
EQPL10TC-1	SBI	10 reactions	EUR 518

SeraMir Exosome RNA Purification kit (5ml ExoQuick and 20 exoRNA columns)
RA806A-1	SBI	20 preps	EUR 493

SeraMir Exosome RNA Purification kit (10ml ExoQuick-TC and 10 exoRNA columns) 10 preps
RA806TC-1	SBI	10 preps	EUR 493

SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns)
RA800TC-1	SBI	10 reactions	EUR 595

Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome)
P101EZ	101Bio	-	Ask for price

Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome)
P100EZ	101Bio	-	Ask for price

Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome) sample
P101EZ--sample	101Bio	-	Ask for price

ExoQuick TC 10ml
EXOTC10A-1	SBI	10 ml	EUR 341

ExoQuick TC 50ml
EXOTC50A-1	SBI	50 ml	EUR 1147

Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome) sample
P100EZ-sample	101Bio	-	Ask for price

Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800TC-1 with 50ml ExoQuick-TC, RA805A-1 and RA812A-1 components)
RA822TC-1	SBI	20 profiles	EUR 1533

TMB Precipitating (High Sensitivity)
TM5125	ScyTek Laboratories	125 ml	EUR 28.28

TMB Precipitating (High Sensitivity)
TM5500	ScyTek Laboratories	500 ml	EUR 70.71

TMB Precipitating (High Sensitivity)
TM5999	ScyTek Laboratories	1000 ml	EUR 127.28

TMB Precipitating Reagent (For Membranes)
TMP125	ScyTek Laboratories	125 ml	EUR 96

TMB Precipitating Reagent (For Membranes)
TMP500	ScyTek Laboratories	500 ml	EUR 159.6

TMB Precipitating Reagent (For Membranes)
TMP999	ScyTek Laboratories	1000 ml	EUR 236.4

TMB Precipitating (Standard Sensitivity)
TM3125	ScyTek Laboratories	125 ml	EUR 24.43

TMB Precipitating (Standard Sensitivity)
TM3500	ScyTek Laboratories	500 ml	EUR 64.28

TMB Precipitating (Standard Sensitivity)
TM3999	ScyTek Laboratories	1000 ml	EUR 117

Universal Protein Precipitating Reagent
BSP012	Bio Basic	100preps	EUR 97.58

ExoQuick ULTRA EV Isolation Kit for Serum and Plasma
EQULTRA-20A-1	SBI	20 reactions	EUR 941

ExoQuick-TC ULTRA EV Isolation Kit for Tissue Culture Media
EQULTRA-20TC-1	SBI	20 reactions	EUR 518

Sulfur Precipitated pure, 98%
69181	Sisco Laboratories	500 Gms	EUR 1.73
Description: Part B

Silicic acid, precipitated
GRM622-500G	EWC Diagnostics	1 unit	EUR 13.55
Description: Silicic acid, precipitated

Sulphur precipitated, Hi-AR™
RM1316-500G	EWC Diagnostics	1 unit	EUR 11.59
Description: Sulphur precipitated, Hi-AR™

ExoQualiTM Exosome DNA Extraction Kit (Pre-Isolated Exosomes)
CEIK-050L-30T	Creative Bioarray	30T	Ask for price

ExoQualiTM Exosome DNA Extraction Kit (Pre-Isolated Exosomes)
CEIK-050L-50T	Creative Bioarray	50T	Ask for price

ExoQualiTM Exosome RNA Extraction Kit (Pre-Isolated Exosomes)
CEIK-051L-30T	Creative Bioarray	30T	Ask for price

ExoQualiTM Exosome RNA Extraction Kit (Pre-Isolated Exosomes)
CEIK-051L-50T	Creative Bioarray	50T	Ask for price

Calcium resinate (precipitated)
C01800	Pfaltz & Bauer	25G	EUR 132.08

Magnesium stearate precipitated fine pow
GRM7219-500G	EWC Diagnostics	1 unit	EUR 6.93
Description: Magnesium stearate precipitated fine pow

Rabbit plasma, barium chloride precipitate
MBS136153-500mL	MyBiosource	500mL	EUR 1360

Rabbit plasma, barium chloride precipitate
MBS136153-50mL	MyBiosource	50mL	EUR 270

Rabbit plasma, barium chloride precipitate
MBS136153-5x500mL	MyBiosource	5x500mL	EUR 5930

ExoQualiTM Exosome Isolation Kit (Urine)
CEIK-003L-10T	Creative Bioarray	10 T	Ask for price

ExoQualiTM Exosome Isolation Kit (Urine)
CEIK-003L-30T	Creative Bioarray	30 T	Ask for price

ExoQualiTM Exosome Isolation Kit (Serum)
CEIK-005L-10T	Creative Bioarray	10 T	Ask for price

ExoQualiTM Exosome Isolation Kit (Serum)
CEIK-005L-30T	Creative Bioarray	30 T	Ask for price

Calcium carbonate, 99%, precipitated, Ph. Eur., BP, USP, FCC grade
GX0868	Glentham Life Sciences	1kg	EUR 30.35

Calcium carbonate, 99%, precipitated, Ph. Eur., BP, USP, FCC grade
GX0868-1	Glentham Life Sciences	1	EUR 33.1

ExoQualiTM Exosome Isolation Kit (Plasma)
CEIK-004L-10T	Creative Bioarray	10 T	Ask for price

ExoQualiTM Exosome Isolation Kit (Plasma)
CEIK-004L-30T	Creative Bioarray	30 T	Ask for price

ExoQualiTM Exosome Chromatography Columns
ESEC-001L-5columns	Creative Bioarray	5 columns	Ask for price

ExoQualiTM Exosome Isolation Kit (Cell Media)
CEIK-001L-10T	Creative Bioarray	10 T	Ask for price

ExoQualiTM Exosome Isolation Kit (Cell Media)
CEIK-001L-30T	Creative Bioarray	30 T	Ask for price

ExoQualiTM Exosome Isolation Kit (All in one)
CEIK-008L-10T	Creative Bioarray	10 T	Ask for price

ExoQualiTM Exosome Isolation Kit (All in one)
CEIK-008L-30T	Creative Bioarray	30 T	Ask for price

ExoQualiTM Max Exosome Chromatography Columns
ESEC-002L-3columns	Creative Bioarray	3 columns	Ask for price

ExoQualiTM Exosome Isolation Kit (Plasma & Serum)
CEIK-006L-10T	Creative Bioarray	10 T	Ask for price

ExoQualiTM Exosome Isolation Kit (Plasma & Serum)
CEIK-006L-30T	Creative Bioarray	30 T	Ask for price

ExoQualiTM Mini Exosome Chromatography Columns
ESEC-003L-2columns	Creative Bioarray	2 columns	Ask for price

ExoQualiTM Exosome Isolation Kit (Stem Cell Media)
CEIK-002L-10T	Creative Bioarray	10 T	Ask for price

ExoQualiTM Exosome Isolation Kit (Stem Cell Media)
CEIK-002L-30T	Creative Bioarray	30 T	Ask for price

ExoQualiTM Exosome Isolation Kit (other body fluids)
CEIK-007L-10T	Creative Bioarray	10 T	Ask for price

ExoQualiTM Exosome Isolation Kit (other body fluids)
CEIK-007L-30T	Creative Bioarray	30 T	Ask for price

ExoQualiTM Exosome DNA Extraction Kit (Plasma & Serum)
CEIK-048L-30T	Creative Bioarray	30T	Ask for price

ExoQualiTM Exosome DNA Extraction Kit (Plasma & Serum)
CEIK-048L-50T	Creative Bioarray	50T	Ask for price

ExoQualiTM Exosome RNA Extraction Kit (Plasma & Serum)
CEIK-093L-30T	Creative Bioarray	30T	Ask for price

ExoQualiTM Exosome RNA Extraction Kit (Plasma & Serum)
CEIK-093L-50T	Creative Bioarray	50T	Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Serum )
CEIK-070L-2vials1x1010particlespervial	Creative Bioarray	2 vials (> 1x10^10 particles per vial)	Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Serum )
CEIK-070L-5vials1x1010particlespervial	Creative Bioarray	5 vials (> 1x10^10 particles per vial)	Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Urine )
CEIK-071L-2vials1x1010particlespervial	Creative Bioarray	2 vials (> 1x10^10 particles per vial)	Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Urine )
CEIK-071L-5vials1x1010particlespervial	Creative Bioarray	5 vials (> 1x10^10 particles per vial)	Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Plasma )
CEIK-068L-2vials1x1010particlespervial	Creative Bioarray	2 vials (> 1x10^10 particles per vial)	Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Plasma )
CEIK-068L-5vials1x1010particlespervial	Creative Bioarray	5 vials (> 1x10^10 particles per vial)	Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Saliva )
CEIK-069L-2vials1x1010particlespervial	Creative Bioarray	2 vials (> 1x10^10 particles per vial)	Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Saliva )
CEIK-069L-5vials1x1010particlespervial	Creative Bioarray	5 vials (> 1x10^10 particles per vial)	Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from serum)
CEIK-044L-30T	Creative Bioarray	30 T	Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from serum)
CEIK-044L-50T	Creative Bioarray	50 T	Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from urine)
CEIK-046L-10T	Creative Bioarray	10 T	Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from urine)
CEIK-046L-24T	Creative Bioarray	24 T	Ask for price

ExoQuali™ Lyophilized Exosome Standards ( MM1 cell line )
CEIK-064L-2vials1x1010particlespervial	Creative Bioarray	2 vials (> 1x10^10 particles per vial)	Ask for price

ExoQuali™ Lyophilized Exosome Standards ( MM1 cell line )
CEIK-064L-5vials1x1010particlespervial	Creative Bioarray	5 vials (> 1x10^10 particles per vial)	Ask for price

ExoQuali™ Lyophilized Exosome Standards ( PC3 cell line )
CEIK-065L-2vials1x1010particlespervial	Creative Bioarray	2 vials (> 1x10^10 particles per vial)	Ask for price

ExoQuali™ Lyophilized Exosome Standards ( PC3 cell line )
CEIK-065L-5vials1x1010particlespervial	Creative Bioarray	5 vials (> 1x10^10 particles per vial)	Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from plasma)
CEIK-045L-30T	Creative Bioarray	30 T	Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from plasma)
CEIK-045L-50T	Creative Bioarray	50 T	Ask for price

ExoQuali™ Lyophilized Exosome Standards ( A549 cell line )
CEIK-054L-2vials1x1010particlespervial	Creative Bioarray	2 vials (> 1x10^10 particles per vial)	Ask for price

ExoQuali™ Lyophilized Exosome Standards ( A549 cell line )
CEIK-054L-5vials1x1010particlespervial	Creative Bioarray	5 vials (> 1x10^10 particles per vial)	Ask for price

ExoQualiTM Total Exosome Capture & Quantification Kit (Serum)
CEIK-107L-96wells	Creative Bioarray	96 wells	Ask for price

ExoQualiTM Total Exosome Capture & Quantification Kit (Urine)
CEIK-110L-96wells	Creative Bioarray	96 wells	Ask for price

ExoQuali™ Lyophilized Exosome Standards ( COLO1 cell line )
CEIK-058L-2vials1x1010particlespervial	Creative Bioarray	2 vials (> 1x10^10 particles per vial)	Ask for price

ExoQuali™ Lyophilized Exosome Standards ( COLO1 cell line )
CEIK-058L-5vials1x1010particlespervial	Creative Bioarray	5 vials (> 1x10^10 particles per vial)	Ask for price

ExoQualiTM Total Exosome Capture & Quantification Kit (Plasma)
CEIK-108L-96wells	Creative Bioarray	96 wells	Ask for price

ExoQuali™ Lyophilized Exosome Standards ( U87 MG cell line )
CEIK-067L-2vials1x1010particlespervial	Creative Bioarray	2 vials (> 1x10^10 particles per vial)	Ask for price

ExoQuali™ Lyophilized Exosome Standards ( U87 MG cell line )
CEIK-067L-5vials1x1010particlespervial	Creative Bioarray	5 vials (> 1x10^10 particles per vial)	Ask for price

ExoQuali™ Lyophilized Exosome Standards ( B16F10 cell line )
CEIK-055L-2vials1x1010particlespervial	Creative Bioarray	2 vials (> 1x10^10 particles per vial)	Ask for price

ExoQuali™ Lyophilized Exosome Standards ( B16F10 cell line )
CEIK-055L-5vials1x1010particlespervial	Creative Bioarray	5 vials (> 1x10^10 particles per vial)	Ask for price

ExoQuali™ Lyophilized Exosome Standards ( BLCL21 cell line )
CEIK-056L-2vials1x1010particlespervial	Creative Bioarray	2 vials (> 1x10^10 particles per vial)	Ask for price

ExoQuali™ Lyophilized Exosome Standards ( BLCL21 cell line )
CEIK-056L-5vials1x1010particlespervial	Creative Bioarray	5 vials (> 1x10^10 particles per vial)	Ask for price

ExoQuali™ Lyophilized Exosome Standards ( HCT116 cell line )
CEIK-060L-2vials1x1010particlespervial	Creative Bioarray	2 vials (> 1x10^10 particles per vial)	Ask for price

ExoQuali™ Lyophilized Exosome Standards ( HCT116 cell line )
CEIK-060L-5vials1x1010particlespervial	Creative Bioarray	5 vials (> 1x10^10 particles per vial)	Ask for price

ExoQuali™ Lyophilized Exosome Standards ( BPH-1 cell line )
CEIK-057L-2vials1x1010particlespervial	Creative Bioarray	2 vials (> 1x10^10 particles per vial)	Ask for price

ExoQuali™ Lyophilized Exosome Standards ( BPH-1 cell line )
CEIK-057L-5vials1x1010particlespervial	Creative Bioarray	5 vials (> 1x10^10 particles per vial)	Ask for price

ExoQuali™ Lyophilized Exosome Standards ( K-562 cell line )
CEIK-062L-2vials1x1010particlespervial	Creative Bioarray	2 vials (> 1x10^10 particles per vial)	Ask for price

ExoQuali™ Lyophilized Exosome Standards ( K-562 cell line )
CEIK-062L-5vials1x1010particlespervial	Creative Bioarray	5 vials (> 1x10^10 particles per vial)	Ask for price

ExoQualiTM Overall Exosome Isolation Immunoplates (from Serum)
CEIK-023L-96wells	Creative Bioarray	96 wells	Ask for price

ExoQualiTM Overall Exosome Isolation Immunoplates (from Saliva)
CEIK-026L-96wells	Creative Bioarray	96 wells	Ask for price

ExoQualiTM Overall Exosome Isolation Immunoplates (from Plasma)
CEIK-095L-96wells	Creative Bioarray	96 wells	Ask for price

ExoQualiTM Overall Exosome Isolation Immunoplates (from Urine)
CEIK-096L-96wells	Creative Bioarray	96 wells	Ask for price

ExoQualiTM Exosome DNA Extraction Kit (Urine & Cell Supernatant)
CEIK-049L-10T	Creative Bioarray	10T	Ask for price

ExoQualiTM Exosome DNA Extraction Kit (Urine & Cell Supernatant)
CEIK-049L-20T	Creative Bioarray	20T	Ask for price

ExoQualiTM Exosome RNA Extraction Kit (Urine & Cell Supernatant)
CEIK-094L-10T	Creative Bioarray	10T	Ask for price

ExoQualiTM Exosome RNA Extraction Kit (Urine & Cell Supernatant)
CEIK-094L-20T	Creative Bioarray	20T	Ask for price

*Innovative Grade US Origin Rabbit Barium Chloride Precipitate Plasma Lyophilized
IRBBACL2PPLALY500ML	Innovative research	each	EUR 1045
Description: *Innovative Grade US Origin Rabbit Barium Chloride Precipitate Plasma Lyophilized

*Innovative Grade US Origin Rabbit Barium Chloride Precipitate Plasma Lyophilized
IRBBACL2PPLALY50ML	Innovative research	each	EUR 150
Description: *Innovative Grade US Origin Rabbit Barium Chloride Precipitate Plasma Lyophilized

ExoQuali™ Lyophilized Exosome Standards ( SK-N-SH cell line )
CEIK-066L-2vials1x1010particlespervial	Creative Bioarray	2 vials (> 1x10^10 particles per vial)	Ask for price

ExoQuali™ Lyophilized Exosome Standards ( SK-N-SH cell line )
CEIK-066L-5vials1x1010particlespervial	Creative Bioarray	5 vials (> 1x10^10 particles per vial)	Ask for price

ExoQualiTM Overall Exosome Isolation Immunoplates (from Cell Media)
CEIK-029L-96wells	Creative Bioarray	96 wells	Ask for price

Wizard Precipitant Synergy - Tubes (Precipitant Synergy Protein Crystallization Screen Kit), 192 formulations, 1.7 mL volumes in tubes
M-R-1009539	MiTeGen	192 X 1.7 ml	EUR 2064
Description: Wizard Precipitant Synergy - Tubes (Precipitant Synergy Protein Crystallization Screen Kit), 192 formulations, 1.7 mL volumes in tubes

ExoQualiTM Overall Exosome Isolation Reagent (from other body fluids)
CEIK-047L-10T	Creative Bioarray	10 T	Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from other body fluids)
CEIK-047L-24T	Creative Bioarray	24 T	Ask for price

ExoQualiTM Total Exosome Capture & Quantification Kit (Cell Supernatant)
CEIK-109L-96wells	Creative Bioarray	96 wells	Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from cell culture media)
CEIK-043L-10T	Creative Bioarray	10 T	Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from cell culture media)
CEIK-043L-24T	Creative Bioarray	24 T	Ask for price

We compared the performance of four cycles of UC (UC4x) with those from two cycles of UC followed by enrichment using EQ (UC2x → EQ) or TEI (UC2x → TEI) kit. The average concentration of smaller EVs isolated from 1 mL of serum using UC2x → EQ (139.0 ± 29.1 mg) and UC2x → TEI (140.4 ± 5.0 mg) was not different from those obtained by using UC4x (141, 8 ± 26.9 mg).