Comparison of methods and characterization of small RNAs from plasma extracellular vesicles of HIV/HCV coinfected patients

The hepatitis C virus (HCV) and human immunodeficiency virus (HIV) hijacks the host machine exosomal addition to the mechanism of infection and evasion of the immune system, modifying a small RNA (smRNA) cargo during infection. Our surface epitope tagged extracellular vesicles (EV) on plasma HIV / HCV-coinfected patients and their cargo smRNA profile, by comparing the different isolation procedures.

six EVS isolation procedures were compared: ultracentrifugation, and five polyethylene glycol based on a different method (commercial, combined with step purification column and two custom); and the two RNA commercial kit (phenol and non-phenol based) are used. High-throughput sequencing of smRNAs do.

Exosomal surface epitopes were analyzed by MACSPlex Exosome Kit. Four miRNAs shown the difference between protocol (HSA-miR-205-5p and HSA-let-7a / b / f-5p). Selection of RNA isolation kits impact on the detection of miRNAs and other smRNAs, where the phenol-based RNA isolation kits are made welcome. EVS enriched surface with HLA-DR / DP / DQ, CD81, and CD8. There are three liver-specific miRNAs expressed (let-7a-5p, miR-21-5p and HSA-miR-122-5p), thus, EVS cargo may reflect the evolution of liver disease.

Another smRNAs like piwi-interacting RNAs were also detected for the first time. Custom-based polyethylene glycol precipitation method combined with phenol-based RNA kit produces a higher number of smRNAs for EVs isolated from patients with HIV / HCV plasma.


micrographs was used to determine the crystal structure, western blotting was used to identify markers exosomal, and NanoSight used to characterize the nanoparticles. The relative expression levels of miRNAs which former is calculated using quantitative PCR and compared between patients with PC and CP. The expression levels of the two former miRNA-21 and miRNA-210 were significantly higher in patients with PC compared to patients with CP (both P <0.001).

However, no significant differences in serum levels are relatively free miR-21 and miR-210 were observed between the 2 groups of patients (both P> 0.05). ex-miRNA-21 and miRNA-210 was associated with tumor stage, as well as other factors. Ex diagnostic potential miRNA-21 and miRNA-210 levels were 83 and 85%, respectively. In addition, when the former Mirna and serum carbohydrate antigen 19-9 combined expression levels, accuracy is increased to 90%. This study identified that the serum former miRNAs, miRNA-21 and miRNA-210 may be of value as a potential biomarker and therapeutic target for the diagnosis and treatment of PC.

 Comparison of methods and characterization of small RNAs from plasma extracellular vesicles of HIV/HCV coinfected patients
Comparison of methods and characterization of small RNAs from plasma extracellular vesicles of HIV/HCV coinfected patients

Evaluation exosome separation of human serum with fractional asymmetric flow field-flow-inlet frit and multiangle light scattering

Exosomes are extracellular vesicles that mediate communication between, the immune response, and tumor metastasis. However, the isolation of blood exosome complex because their size and density similar to blood lipoprotein.

ExoQuick Exosome RNA Column Purification kit

EQ808A-1 20 preps
EUR 402.00

ExoQuick ULTRA EV Isolation Kit for Serum and Plasma

EQULTRA-20A-1 20 reactions
EUR 579.00

Exosome precipitation from Plasma and Serum

EPStep-PS 5 ml
EUR 297.00

ExoQuick Exosome Isolation and RNA Purification kit (for Tissue Culture Media)

EQ806TC-1 10 preps
EUR 554.00

SeraMir Exosome RNA Purification kit (5ml ExoQuick and 20 exoRNA columns)

RA806A-1 20 preps
EUR 554.00
  • Category: MicroRNA Tools

ExoQuick exosome precipitation solution (20 ml)

EXOQ20A-1 20 ml
EUR 1179.00
  • Category: Exosomes

ExoQuick exosome precipitation solution (5 ml)

EXOQ5A-1 5 ml
EUR 400.00
  • Category: Exosomes

Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL)

TMEXO-1 100 reactions
EUR 583.00
  • Category: Exosomes

XCF COMPLETE Exosome and cfDNA Isolation Kit (for Serum and Plasma)

XCF100A-1 20 rxn
EUR 647.00
  • Category: Exosome

SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns)

RA800TC-1 10 reactions
EUR 659.00
  • Category: MicroRNA Tools

ExoQuick-LP for lipoprotein pre-clear & exosome isolation

EXOLP5A-1 5 reactions
EUR 533.00
  • Category: Exosomes

SeraMir Exosome RNA Purification kit (10ml ExoQuick-TC and 10 exoRNA columns) 10 preps

RA806TC-1 10 preps
EUR 554.00
  • Category: MicroRNA Tools

Exosome Isolation kit (for serum & plasma -Exosome-RNA-NGS)

P101NGS NULL
EUR NULL

ExoQuick-TC PLUS Exosome Purification Kit - for tissue culture media

EQPL10TC-1 10 reactions
EUR 579.00
  • Category: Exosomes

Exosome Isolation kit (for serum & plasma)

P101 NULL
EUR NULL

Exosome Isolation kit (for serum & plasma)

P101L NULL
EUR NULL

Exosome Isolation kit (for serum & plasma)

P101S NULL
EUR NULL

ExoQuick-CG cGMP-grade Exosome Isolation reagent (50 ml)

EXOCG50A-1 50 ml
EUR 1181.00
  • Category: Exosomes

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Plasma, Luminometric)

K1206-100
EUR 968.00

Exosome precipitation from Plasma + Thrombin

EPStep-T 5 ml
EUR 336.00

ExoStd? Plasma Fluorescent Exosome Standard

M1073-100
EUR 756.00

Lyophilized Exosome Standard (Human Plasma)

ESL-25 30 µgx2 vials
EUR 1337.00
Description: Lyophilized exosomes can be used as control standards for FACS, WB, ELISA and as calibration standards for quantitation of exosome-derived markers from biological samples.

ExoQuant? Overall Exosome Capture and Quantification ELISA Assay Kit (Human Plasma, Colorimetric)

K1201-100
EUR 968.00

VEX Exosome Isolation Reagent (from plasma)

R603 10 ml
EUR 641.00

ExoDNAPS? Circulating and Exosome-associated DNA Extraction Kit (Human Plasma/Serum, 20 reactions)

K1230-20
EUR 718.00

ExoDNAPS? Circulating and Exosome-associated DNA Extraction Kit (Human Plasma/Serum, 40 reactions)

K1230-40
EUR 1061.00

Exosome Purification and Detection Kit (Urine)

abx290027-25tests 25 tests
EUR 648.00
  • Shipped within 2-3 weeks.

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit

CAS400A-KIT 1 kit (10 rxn)
EUR 1110.00
  • Category: Cas9

Prep/ Rat Prep ELISA Kit

ELI-36167r 96 Tests
EUR 886.00

TumorExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay)

M1016-100-T
EUR 729.00

TumorExoPure? Immunoplates (Exosome Isolation, plasma, luminometric assay)

M1017-100-W
EUR 740.00

TumorExoPure? Immunoplates (Exosome Isolation, plasma, fluorimetric assay)

M1018-100-B
EUR 740.00

NeuralExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay)

M1019-100-T
EUR 756.00

NeuralExoPure? Immunoplates (Exosome Isolation, plasma, luminometric assay)

M1020-100-W
EUR 740.00

NeuralExoPure? Immunoplates (Exosome Isolation, plasma, fluorimetric assay)

M1021-100-B
EUR 740.00

GlialExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay)

M1022-100-T
EUR 740.00

GlialExoPure? Immunoplates (Exosome Isolation, plasma, luminometric assay)

M1023-100-W
EUR 740.00

GlialExoPure? Immunoplates (Exosome Isolation, plasma, fluorimetric assay)

M1024-100-B
EUR 740.00

ExoQuick TC 10ml

EXOTC10A-1 10 ml
EUR 399.00
  • Category: Exosomes

ExoQuick TC 50ml

EXOTC50A-1 50 ml
EUR 1222.00
  • Category: Exosomes

Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome)

P101EZ NULL
EUR NULL

Exosome Purification and Detection Kit (Cell Culture)

abx290025-25tests 25 tests
EUR 648.00
  • Shipped within 2-3 weeks.

Monocytes/PlateletExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay)

M1025-100-T
EUR 740.00

Monocytes/PlateletExoPure? Immunoplates (Exosome Isolation, plasma, luminometric assay)

M1026-100-W
EUR 740.00

Monocytes/PlateletExoPure? Immunoplates (Exosome Isolation, plasma, fluorimetric assay)

M1027-100-B
EUR 740.00

Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800TC-1 with 50ml ExoQuick-TC, RA805A-1 and RA812A-1 components)

RA822TC-1 20 profiles
EUR 1618.00
  • Category: MicroRNA Tools

Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome) sample

P101EZ--sample NULL
EUR NULL

Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]

CAS9LIG-KIT 1 Kit
EUR 153.00
  • Category: Cas9

ExoPure? Immunoplates (Overall Exosome Isolation, plasma, urine, colorimetric assay)

M1004-100-T
EUR 947.00

ExoPure? Immunoplates (Overall Exosome Isolation, plasma, urine, luminometric assay)

M1005-100-W
EUR 740.00

ExoPure? Immunoplates (Overall Exosome Isolation, plasma, urine, fluorometric assay)

M1006-100-B
EUR 740.00

ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum)

M1038-10
EUR 838.00

ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum)

M1038-20
EUR 1289.00

ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum)

M1038-3
EUR 436.00

ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum)

M1038-5
EUR 664.00

ExoStd? Lyophilized Exosome Standard (30 µg, Human Plasma, 2 vials)

M1040-2
EUR 593.00

ExoStd? Lyophilized Exosome Standard (30 µg, Human Plasma, 4 vials)

M1040-4
EUR 909.00

ExoStd? Lyophilized Exosome Standard (30 µg, Human Plasma, 6 vials)

M1040-6
EUR 1300.00

ExoStd? Lyophilized Exosome Standard (100 µg, Human Plasma, 2 vials)

M1041-2
EUR 711.00

ExoStd? Lyophilized Exosome Standard (100 µg, Human Plasma, 4 vials)

M1041-4
EUR 1137.00

ExoStd? Lyophilized Exosome Standard (100 µg, Human Plasma, 6 vials)

M1041-6
EUR 1572.00

PREP ELISA KIT|Human

EF002043 96 Tests
EUR 689.00

ProtoGel Sample Prep Kit

NAT1248 EACH
EUR 186.00

PREP siRNA

20-abx904225
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

PREP Antibody

20-abx327717
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

PREP siRNA

20-abx929702
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

PREP siRNA

20-abx929703
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

PREP antibody

70R-4323 50 ug
EUR 467.00
Description: Rabbit polyclonal PREP antibody raised against the N terminal of PREP

PREP antibody

70R-4324 50 ug
EUR 467.00
Description: Rabbit polyclonal PREP antibody raised against the middle region of PREP

PREP antibody

70R-19508 50 ul
EUR 435.00
Description: Rabbit polyclonal PREP antibody

PREP Antibody

1-CSB-PA004861
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Description: A polyclonal antibody against PREP. Recognizes PREP from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000

PREP Antibody

1-CSB-PA018664GA01HU
  • EUR 597.00
  • EUR 333.00
  • 150ul
  • 50ul
  • Form: Liquid
  • Buffer: PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. -20℃, Avoid freeze / thaw cycles. Antigen Affinity Purified
Description: A polyclonal antibody against PREP. Recognizes PREP from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC

PREP Antibody

1-CSB-PA018664LA01HU
  • EUR 317.00
  • EUR 335.00
  • 100ul
  • 50ul
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 Antigen Affinity Purified
Description: A polyclonal antibody against PREP. Recognizes PREP from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200

anti-PREP

YF-PA27335 50 ug
EUR 363.00
Description: Mouse polyclonal to PREP

CLOuD9 Gene Expression Regulation Kit (includes 10 ug each of dCas9-PYL1 and dCas9-ABI1 lentivectors, and 100 ul of 0.5M Inducer Agent)

CASCL9-100A-KIT 1 Kit
EUR 1132.00
  • Category: Cas9

Zenoquick Nucleosomal DNA Prep Kit

Z2010-020 20 preps
EUR 286.00

FluoroCet Exosome Quantitation Kit

FCET96A-1 96 reactions
EUR 726.00
  • Category: Exosomes

EXOCET Exosome Quantitation Kit

EXOCET96A-1 96 reactions
EUR 521.00
  • Category: Exosomes

Exosome-TEM-easy Kit

P130 NULL
EUR NULL

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Urine, Colorimetric)

K1202-100
EUR 968.00

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Serum, Colorimetric)

K1203-100
EUR 968.00

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Cell media, Colorimetric)

K1205-100
EUR 968.00

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Urine, Luminometric)

K1207-100
EUR 968.00

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Serum, Luminometric)

K1208-100
EUR 968.00

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Cell Media, Luminometric)

K1210-100
EUR 968.00

TumorExoRNA? Tumor-derived exosome immunocapture and RNA extraction kit (10 reactions)

K1221-10
EUR 827.00

TumorExoRNA? Tumor-derived exosome immunocapture and RNA extraction kit (20 reactions)

K1221-20
EUR 1219.00

PREP Polyclonal Antibody

E-AB-33402-120uL 120uL
EUR 257.00
  • Conjugation: Unconjugated
  • Buffer composition: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
  • Purified by: Affinity purification
  • Background: The protein encoded by this gene is a cytosolic prolyl endopeptidase that cleaves peptide bo
  • Show more
Description: Rabbit antibody against Human,Mouse,Rat PREP for WB,IHC-p,ELISA applications.

PREP Polyclonal Antibody

E-AB-33402-20uL 20uL
EUR 130.00
  • Conjugation: Unconjugated
  • Buffer composition: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
  • Purified by: Affinity purification
  • Background: The protein encoded by this gene is a cytosolic prolyl endopeptidase that cleaves peptide bo
  • Show more
Description: Rabbit antibody against Human,Mouse,Rat PREP for WB,IHC-p,ELISA applications.

PREP Polyclonal Antibody

E-AB-33402-60uL 60uL
EUR 175.00
  • Conjugation: Unconjugated
  • Buffer composition: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
  • Purified by: Affinity purification
  • Background: The protein encoded by this gene is a cytosolic prolyl endopeptidase that cleaves peptide bo
  • Show more
Description: Rabbit antibody against Human,Mouse,Rat PREP for WB,IHC-p,ELISA applications.

PREP Polyclonal Antibody

ABP52863-003ml 0.03ml
EUR 158.00
  • Immunogen information: Synthesized peptide derived from the N-terminal region of human PREP at AA range: 10-90
  • Applications tips:
Description: A polyclonal antibody for detection of PREP from Human, Mouse, Rat. This PREP antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human PREP at AA range: 10-90

PREP Polyclonal Antibody

ABP52863-01ml 0.1ml
EUR 289.00
  • Immunogen information: Synthesized peptide derived from the N-terminal region of human PREP at AA range: 10-90
  • Applications tips:
Description: A polyclonal antibody for detection of PREP from Human, Mouse, Rat. This PREP antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human PREP at AA range: 10-90

PREP Polyclonal Antibody

ABP52863-02ml 0.2ml
EUR 414.00
  • Immunogen information: Synthesized peptide derived from the N-terminal region of human PREP at AA range: 10-90
  • Applications tips:
Description: A polyclonal antibody for detection of PREP from Human, Mouse, Rat. This PREP antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human PREP at AA range: 10-90

PREP Rabbit pAb

A9838-100ul 100 ul
EUR 308.00

PREP Rabbit pAb

A9838-200ul 200 ul
EUR 459.00

PREP Rabbit pAb

A9838-20ul 20 ul
EUR 183.00

PREP Rabbit pAb

A9838-50ul 50 ul
EUR 223.00

PREP Blocking Peptide

33R-5028 100 ug
EUR 180.00
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of PREP antibody, catalog no. 70R-4324

PREP Blocking Peptide

33R-9102 100 ug
EUR 180.00
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of PREP antibody, catalog no. 70R-4323

PREP Polyclonal Antibody

31751-100ul 100ul
EUR 252.00

PREP Polyclonal Antibody

31751-50ul 50ul
EUR 187.00

PREP Polyclonal Antibody

ABP59997-003ml 0.03ml
EUR 158.00
  • Immunogen information: Synthesized peptide derived from part region of human PREP protein at amino acid sequence of 450-530
  • Applications tips:
Description: A polyclonal antibody for detection of PREP from Human, Mouse. This PREP antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human PREP protein at amino acid sequence of 450-530

PREP Polyclonal Antibody

ABP59997-01ml 0.1ml
EUR 289.00
  • Immunogen information: Synthesized peptide derived from part region of human PREP protein at amino acid sequence of 450-530
  • Applications tips:
Description: A polyclonal antibody for detection of PREP from Human, Mouse. This PREP antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human PREP protein at amino acid sequence of 450-530

PREP Polyclonal Antibody

ABP59997-02ml 0.2ml
EUR 414.00
  • Immunogen information: Synthesized peptide derived from part region of human PREP protein at amino acid sequence of 450-530
  • Applications tips:
Description: A polyclonal antibody for detection of PREP from Human, Mouse. This PREP antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human PREP protein at amino acid sequence of 450-530

anti- PREP antibody

FNab06767 100µg
EUR 548.75
  • Immunogen: prolyl endopeptidase
  • Uniprot ID: P48147
  • Gene ID: 5550
  • Research Area: Metabolism
Description: Antibody raised against PREP

PREP Polyclonal Antibody

ES11287-100ul 100ul
EUR 279.00
Description: A Rabbit Polyclonal antibody against PREP from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA

PREP Polyclonal Antibody

ES11287-50ul 50ul
EUR 207.00
Description: A Rabbit Polyclonal antibody against PREP from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA

PREP Polyclonal Antibody

ES3862-100ul 100ul
EUR 279.00
Description: A Rabbit Polyclonal antibody against PREP from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

PREP Polyclonal Antibody

ES3862-50ul 50ul
EUR 207.00
Description: A Rabbit Polyclonal antibody against PREP from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

Anti-PREP antibody

PAab06767 100 ug
EUR 386.00

Anti-PREP antibody

STJ111880 100 µl
EUR 277.00
Description: The protein encoded by this gene is a cytosolic prolyl endopeptidase that cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Prolyl endopeptidases have been reported to be involved in the maturation and degradation of peptide hormones and neuropeptides.

Anti-PREP antibody

STJ96497 200 µl
EUR 197.00
Description: Rabbit polyclonal to PREP.

Anti-PREP antibody

STJ192445 200 µl
EUR 197.00
Description: Unconjugated Rabbit polyclonal to PREP

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum, 10 reactions)

M1039-10
EUR 838.00

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum, 20 reactions)

M1039-20
EUR 1289.00

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum, 3 reactions)

M1039-3
EUR 436.00

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum, 5 reactions)

M1039-5
EUR 664.00

Psoriasis Exosome

P141-PS NULL
EUR NULL

AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE620A-KIT 1 kit
EUR 2132.00
  • Category: Gene Editing

ExoFectin Plasmid DNA-into-Exosome Kit (DNA transfection kit for exosome)

P402 NULL
EUR NULL

Frit Kit

FRIT-KIT 1each
EUR 124.00
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE622A-KIT 1 kit
EUR 2132.00
  • Category: Gene Editing

AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE624A-KIT 1 kit
EUR 2132.00
  • Category: Gene Editing

Column Packing Kit

PACK-KIT 1pack
EUR 1035.00
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 266.00

Rabbit Prolyl endopeptidase(PREP) ELISA kit

E04P0865-192T 192 tests
EUR 1270.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Prolyl endopeptidase(PREP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Prolyl endopeptidase(PREP) ELISA kit

E04P0865-48 1 plate of 48 wells
EUR 520.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Prolyl endopeptidase(PREP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Prolyl endopeptidase(PREP) ELISA kit

E04P0865-96 1 plate of 96 wells
EUR 685.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Prolyl endopeptidase(PREP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Prolyl endopeptidase(PREP) ELISA kit

E03P0865-192T 192 tests
EUR 1270.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Mouse Prolyl endopeptidase(PREP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Prolyl endopeptidase(PREP) ELISA kit

E03P0865-48 1 plate of 48 wells
EUR 520.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Mouse Prolyl endopeptidase(PREP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Prolyl endopeptidase(PREP) ELISA kit

E03P0865-96 1 plate of 96 wells
EUR 685.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Mouse Prolyl endopeptidase(PREP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Prolyl endopeptidase(PREP) ELISA kit

E02P0865-192T 192 tests
EUR 1270.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Rat Prolyl endopeptidase(PREP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Prolyl endopeptidase(PREP) ELISA kit

E02P0865-48 1 plate of 48 wells
EUR 520.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Rat Prolyl endopeptidase(PREP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Prolyl endopeptidase(PREP) ELISA kit

E02P0865-96 1 plate of 96 wells
EUR 685.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Prolyl endopeptidase(PREP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Prolyl endopeptidase(PREP) ELISA kit

E01P0865-192T 192 tests
EUR 1270.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Human Prolyl endopeptidase(PREP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Prolyl endopeptidase(PREP) ELISA kit

E01P0865-48 1 plate of 48 wells
EUR 520.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Human Prolyl endopeptidase(PREP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Prolyl endopeptidase(PREP) ELISA kit

E01P0865-96 1 plate of 96 wells
EUR 685.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Human Prolyl endopeptidase(PREP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Prolyl endopeptidase (PREP) ELISA Kit

20-abx390308
  • EUR 7378.00
  • EUR 3933.00
  • EUR 911.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Shipped within 5-12 working days.

Rat Prolyl endopeptidase (PREP) ELISA Kit

20-abx391848
  • EUR 7378.00
  • EUR 3933.00
  • EUR 911.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Shipped within 5-12 working days.

Cow Prolyl endopeptidase (PREP) ELISA Kit

20-abx380739
  • EUR 7911.00
  • EUR 4215.00
  • EUR 973.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Shipped within 5-12 working days.

Dog Prolyl endopeptidase(PREP) ELISA kit

E08P0865-192T 192 tests
EUR 1270.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Canine Prolyl endopeptidase(PREP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Prolyl endopeptidase(PREP) ELISA kit

E08P0865-48 1 plate of 48 wells
EUR 520.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Canine Prolyl endopeptidase(PREP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Prolyl endopeptidase(PREP) ELISA kit

E08P0865-96 1 plate of 96 wells
EUR 685.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Canine Prolyl endopeptidase(PREP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Prolyl endopeptidase(PREP) ELISA kit

E09P0865-192T 192 tests
EUR 1270.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Monkey Prolyl endopeptidase(PREP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Prolyl endopeptidase(PREP) ELISA kit

E09P0865-48 1 plate of 48 wells
EUR 520.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Monkey Prolyl endopeptidase(PREP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Prolyl endopeptidase(PREP) ELISA kit

E09P0865-96 1 plate of 96 wells
EUR 685.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Monkey Prolyl endopeptidase(PREP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Prolyl endopeptidase(PREP) ELISA kit

E07P0865-192T 192 tests
EUR 1270.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Porcine Prolyl endopeptidase(PREP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Prolyl endopeptidase(PREP) ELISA kit

E07P0865-48 1 plate of 48 wells
EUR 520.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Porcine Prolyl endopeptidase(PREP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Prolyl endopeptidase(PREP) ELISA kit

E07P0865-96 1 plate of 96 wells
EUR 685.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Porcine Prolyl endopeptidase(PREP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Prolyl endopeptidase (PREP) ELISA Kit

20-abx382454
  • EUR 7378.00
  • EUR 3933.00
  • EUR 911.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Shipped within 5-12 working days.

Human Prolyl endopeptidase, PREP ELISA KIT

ELI-45388h 96 Tests
EUR 824.00

Mouse Prolyl endopeptidase, Prep ELISA KIT

ELI-37820m 96 Tests
EUR 865.00

Goat Prolyl endopeptidase(PREP) ELISA kit

E06P0865-192T 192 tests
EUR 1270.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Goat Prolyl endopeptidase(PREP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Prolyl endopeptidase(PREP) ELISA kit

E06P0865-48 1 plate of 48 wells
EUR 520.00
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Goat Prolyl endopeptidase(PREP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Here, we employed programming field frit-inlet fractionation asymmetrical flow field-flow (FIAF4) coupled with light scattering multiangle (MALS) for effective separation of exosomes from the protein bound to the free and lipoproteins present in serum samples using the methods of pre-treatment is different, namely , a commercial kit exosome isolation, ultracentrifugation (UC), and a simple centrifugation followed by ultrafiltration (UF) .Sizes of exosomes eluted, as calculated by the MALS signal, approximated well with the results of dynamic light scattering batch of fraction was collected and the particle size of polystyrene