Comparison of methods and characterization of small RNAs from plasma extracellular vesicles of HIV/HCV coinfected patients

The hepatitis C virus (HCV) and human immunodeficiency virus (HIV) hijacks the host machine exosomal addition to the mechanism of infection and evasion of the immune system, modifying a small RNA (smRNA) cargo during infection. Our surface epitope tagged extracellular vesicles (EV) on plasma HIV / HCV-coinfected patients and their cargo smRNA profile, by comparing the different isolation procedures.

six EVS isolation procedures were compared: ultracentrifugation, and five polyethylene glycol based on a different method (commercial, combined with step purification column and two custom); and the two RNA commercial kit (phenol and non-phenol based) are used. High-throughput sequencing of smRNAs do.

Exosomal surface epitopes were analyzed by MACSPlex Exosome Kit. Four miRNAs shown the difference between protocol (HSA-miR-205-5p and HSA-let-7a / b / f-5p). Selection of RNA isolation kits impact on the detection of miRNAs and other smRNAs, where the phenol-based RNA isolation kits are made welcome. EVS enriched surface with HLA-DR / DP / DQ, CD81, and CD8. There are three liver-specific miRNAs expressed (let-7a-5p, miR-21-5p and HSA-miR-122-5p), thus, EVS cargo may reflect the evolution of liver disease.

Another smRNAs like piwi-interacting RNAs were also detected for the first time. Custom-based polyethylene glycol precipitation method combined with phenol-based RNA kit produces a higher number of smRNAs for EVs isolated from patients with HIV / HCV plasma.


micrographs was used to determine the crystal structure, western blotting was used to identify markers exosomal, and NanoSight used to characterize the nanoparticles. The relative expression levels of miRNAs which former is calculated using quantitative PCR and compared between patients with PC and CP. The expression levels of the two former miRNA-21 and miRNA-210 were significantly higher in patients with PC compared to patients with CP (both P <0.001).

However, no significant differences in serum levels are relatively free miR-21 and miR-210 were observed between the 2 groups of patients (both P> 0.05). ex-miRNA-21 and miRNA-210 was associated with tumor stage, as well as other factors. Ex diagnostic potential miRNA-21 and miRNA-210 levels were 83 and 85%, respectively. In addition, when the former Mirna and serum carbohydrate antigen 19-9 combined expression levels, accuracy is increased to 90%. This study identified that the serum former miRNAs, miRNA-21 and miRNA-210 may be of value as a potential biomarker and therapeutic target for the diagnosis and treatment of PC.

 Comparison of methods and characterization of small RNAs from plasma extracellular vesicles of HIV/HCV coinfected patients
Comparison of methods and characterization of small RNAs from plasma extracellular vesicles of HIV/HCV coinfected patients

Evaluation exosome separation of human serum with fractional asymmetric flow field-flow-inlet frit and multiangle light scattering

Exosomes are extracellular vesicles that mediate communication between, the immune response, and tumor metastasis. However, the isolation of blood exosome complex because their size and density similar to blood lipoprotein.

ExoQuick Exosome Isolation and RNA Purification kit (for Tissue Culture Media)

EQ806TC-1 10 preps
EUR 493

ExoQuick-LP for lipoprotein pre-clear & exosome isolation

EXOLP5A-1 5 reactions
EUR 473

ExoQuick-CG cGMP-grade Exosome Isolation reagent (50 ml)

EXOCG50A-1 50 ml
EUR 1106

SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns)

RA800TC-1 10 reactions
EUR 595

ExoQuick-TC PLUS Exosome Purification Kit - for tissue culture media

EQPL10TC-1 10 reactions
EUR 518

ExoQuick ULTRA EV Isolation Kit for Serum and Plasma

EQULTRA-20A-1 20 reactions
EUR 941

Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800TC-1 with 50ml ExoQuick-TC, RA805A-1 and RA812A-1 components)

RA822TC-1 20 profiles
EUR 1533

Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL)

TMEXO-1 100 reactions
EUR 522

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Plasma, Luminometric)

K1206-100 each
EUR 1161.6

Exosome precipitation from Plasma and Serum

EPStep-PS 5 ml
EUR 356.4

ExoQuant? Overall Exosome Capture and Quantification ELISA Assay Kit (Human Plasma, Colorimetric)

K1201-100 each
EUR 1161.6

Exosome Purification and Detection Kit (Plasma)

abx290026-25tests 25 tests
EUR 777.6

ExoQuick TC 10ml

EXOTC10A-1 10 ml
EUR 341

ExoQuick TC 50ml

EXOTC50A-1 50 ml
EUR 1147

Exosome Isolation kit (for serum & plasma -Exosome-RNA-NGS)

P101NGS - Ask for price

XCF COMPLETE Exosome and cfDNA Isolation Kit (for Serum and Plasma)

XCF100A-1 20 rxn
EUR 583

Exosome Isolation kit (for serum & plasma)

P101 - Ask for price

Exosome Isolation kit (for serum & plasma)

P101L - Ask for price

Exosome Isolation kit (for serum & plasma)

P101S - Ask for price

Exosome precipitation from Plasma + Thrombin

EPStep-T 5 ml
EUR 403.2

ExoStd? Plasma Fluorescent Exosome Standard

M1073-100 each
EUR 907.2

Lyophilized Exosome Standard (Human Plasma)

ESL-25 30 µgx2 vials
EUR 1604.4
Description: Lyophilized exosomes can be used as control standards for FACS, WB, ELISA and as calibration standards for quantitation of exosome-derived markers from biological samples.

Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome)

P101EZ - Ask for price

VEX Exosome Isolation Reagent (from plasma)

R603 10 ml
EUR 769.2

ExoQuick-TC ULTRA EV Isolation Kit for Tissue Culture Media

EQULTRA-20TC-1 20 reactions
EUR 518

Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome) sample

P101EZ--sample - Ask for price

ExoDNAPS? Circulating and Exosome-associated DNA Extraction Kit (Human Plasma/Serum, 20 reactions)

K1230-20 each
EUR 861.6

ExoDNAPS? Circulating and Exosome-associated DNA Extraction Kit (Human Plasma/Serum, 40 reactions)

K1230-40 each
EUR 1273.2

ExoFACS? Kit for Plasma Exosomes

K1232-20 each
EUR 952.8

ExoStd? Lyophilized Exosome Standard (30 µg, Human Plasma, 2 vials)

M1040-2 each
EUR 711.6

ExoStd? Lyophilized Exosome Standard (30 µg, Human Plasma, 4 vials)

M1040-4 each
EUR 1090.8

ExoStd? Lyophilized Exosome Standard (30 µg, Human Plasma, 6 vials)

M1040-6 each
EUR 1560

ExoStd? Lyophilized Exosome Standard (100 µg, Human Plasma, 2 vials)

M1041-2 each
EUR 853.2

ExoStd? Lyophilized Exosome Standard (100 µg, Human Plasma, 4 vials)

M1041-4 each
EUR 1364.4

ExoStd? Lyophilized Exosome Standard (100 µg, Human Plasma, 6 vials)

M1041-6 each
EUR 1886.4

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Cell Media, Luminometric)

K1210-100 each
EUR 1161.6

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Cell media, Colorimetric)

K1205-100 each
EUR 1161.6

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Urine, Colorimetric)

K1202-100 each
EUR 1161.6

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Serum, Colorimetric)

K1203-100 each
EUR 1161.6

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Urine, Luminometric)

K1207-100 each
EUR 1161.6

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Serum, Luminometric)

K1208-100 each
EUR 1161.6

TumorExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay)

M1016-100-T each
EUR 874.8

TumorExoPure? Immunoplates (Exosome Isolation, plasma, luminometric assay)

M1017-100-W each
EUR 888

TumorExoPure? Immunoplates (Exosome Isolation, plasma, fluorimetric assay)

M1018-100-B each
EUR 888

GlialExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay)

M1022-100-T each
EUR 888

GlialExoPure? Immunoplates (Exosome Isolation, plasma, luminometric assay)

M1023-100-W each
EUR 888

GlialExoPure? Immunoplates (Exosome Isolation, plasma, fluorimetric assay)

M1024-100-B each
EUR 888

NeuralExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay)

M1019-100-T each
EUR 907.2

NeuralExoPure? Immunoplates (Exosome Isolation, plasma, luminometric assay)

M1020-100-W each
EUR 888

NeuralExoPure? Immunoplates (Exosome Isolation, plasma, fluorimetric assay)

M1021-100-B each
EUR 888

ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum)

M1038-10 each
EUR 1005.6

ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum)

M1038-20 each
EUR 1546.8

ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum)

M1038-3 each
EUR 523.2

ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum)

M1038-5 each
EUR 796.8

ExoPure? Immunoplates (Overall Exosome Isolation, plasma, urine, colorimetric assay)

M1004-100-T each
EUR 1136.4

ExoPure? Immunoplates (Overall Exosome Isolation, plasma, urine, luminometric assay)

M1005-100-W each
EUR 888

ExoPure? Immunoplates (Overall Exosome Isolation, plasma, urine, fluorometric assay)

M1006-100-B each
EUR 888

Monocytes/PlateletExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay)

M1025-100-T each
EUR 888

Monocytes/PlateletExoPure? Immunoplates (Exosome Isolation, plasma, luminometric assay)

M1026-100-W each
EUR 888

Monocytes/PlateletExoPure? Immunoplates (Exosome Isolation, plasma, fluorimetric assay)

M1027-100-B each
EUR 888

Human Neural derived plasma exosomes ELISA Kit

QY-E05473 96T
EUR 433.2

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum, 10 reactions)

M1039-10 each
EUR 1005.6

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum, 20 reactions)

M1039-20 each
EUR 1546.8

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum, 3 reactions)

M1039-3 each
EUR 523.2

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum, 5 reactions)

M1039-5 each
EUR 796.8

ExoQuant? Tumor-derived Exosome enrichment and Quantification Assay Kit (Biological Fluids/Cell Media, Colorimetric)

K1204-100 each
EUR 1161.6

ExoQuant? Tumor-derived Exosome enrichment and Quantification Assay Kit (Biological Fluids/Cell Media, Luminometric)

K1209-100 each
EUR 1161.6

Exosome Purification and Detection Kit (Urine)

abx290027-25tests 25 tests
EUR 777.6

ExoELISA-Step for detection and quantification of exosomes from human serum/plasma

Exo2508 96
EUR 654.5
Description: ExoELISA-Step for detection and quantification of exosomes from human serum/plasma

Exosome RNA and protein extraction

S103 -
EUR 1200

ExoFectin Plasmid DNA-into-Exosome Kit (DNA transfection kit for exosome)

P402 - Ask for price

Exosome Purification and Detection Kit (Cell Culture)

abx290025-25tests 25 tests
EUR 777.6

Exosome Isolation Kit

E601E001 20 tests
EUR 2500

Exosome-TEM-easy Kit

P130 - Ask for price

EXOCET Exosome Quantitation Kit

EXOCET96A-1 96 reactions
EUR 462

Exosome precipitation Solution

EPStep 12 ml
EUR 333

Psoriasis Exosome

P141-PS - Ask for price

Exosome (Pre-isolated) RNA Extraction kit Note! This kit doesn't contain the conponents for exosome enrichment.

EIRK-01 50 reactions
EUR 3380.4
Description: A RNA Extraction kit that is ideal for total RNA (miRNA+mRNAs) extraction from pre-isolated exosomes.

Exo-Fect Exosome Transfection Kit

EXFT10A-1 10 reactions
EUR 238

Exo-Fect Exosome Transfection Kit

EXFT20A-1 20 reactions
EUR 429

Lung Cancer Exosome

P141-LG - Ask for price

Exosome Isolation kit (for urine)

P120L - Ask for price

Exosome Isolation kit (for urine)

P120S - Ask for price

LiqBiopsy Tumor Exosome RNA Kit

K1254-24 each
EUR 1332

FluoroCet Exosome Quantitation Kit

FCET96A-1 96 reactions
EUR 661

Leukemia - ALL Exosome

P141-ALL - Ask for price

Leukemia - AML Exosome

P141-AML - Ask for price

Leukemia - CLL Exosome

P141-CLL - Ask for price

Leukemia - CML Exosome

P141-CML - Ask for price

Liver Cancer Exosome

P141-LV - Ask for price

Exosome FACS sorting

S107 -
EUR 2000

Breast Cancer Exosome

P141-BR - Ask for price

Crohn's Disease Exosome

P141-CR - Ask for price

Kidney Cancer Exosome

P141-KN - Ask for price

Psoriasis Exosome RNA

P241-PS - Ask for price

Bladder Cancer Exosome

P141-BL - Ask for price

Thyroid Cancer Exosome

P141-TH - Ask for price

GPC1 Capture Exosome Isolation Kit

E601E004 20 tests
EUR 2500

CD63 Capture Exosome Isolation Kit

E601E005 20 tests
EUR 2500

CD81 Capture Exosome Isolation Kit

E601E006 20 tests
EUR 2500

Prostate Cancer Exosome

P141-PR - Ask for price

Exosome Biotin labeling

S106 -
EUR 800

Breast cancer Exosome Isolation Kit

E601E002 20 tests
EUR 2500

EPCAM Capture Exosome Isolation Kit

E601E003 20 tests
EUR 2500

HSP70 Capture Exosome Isolation Kit

E601E007 20 tests
EUR 2500

ExoDNAPS? Conc Exosome DNA Extraction Kit

K1243-20 each
EUR 992.4

ExoDNAPS? Conc Exosome DNA Extraction Kit

K1243-40 each
EUR 1474.8

ExoDNAUC? Conc Exosome DNA Extraction Kit

K1244-20 each
EUR 992.4

ExoDNAUC? Conc Exosome DNA Extraction Kit

K1244-40 each
EUR 1474.8

LiqBiopsy Tumor Exosome DNA Kit I

K1255-20 each
EUR 1201.2

LiqBiopsy Tumor Exosome DNA Kit II

K1256-20 each
EUR 1266

SeraMir Exosome RNA Ampflication Kit

RA800A-1 20 reactions
EUR 846

Human Exosome Component 2 (EXOSC2) CLIA Kit

20-abx495555
  • EUR 9567.60
  • EUR 5095.20
  • EUR 1177.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Exosome Component 7 (EXOSC7) CLIA Kit

20-abx496063
  • EUR 10282.80
  • EUR 5472.00
  • EUR 1262.40
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Colorectal Cancer Exosome

P141-CO - Ask for price

Exosome antibody labeling

S105 -
EUR 800

Lung Cancer Exosome RNA

P241-LG - Ask for price

Human Exosome Component 2 (EXOSC2) ELISA Kit

20-abx151457
  • EUR 8853.60
  • EUR 4719.60
  • EUR 1093.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Exosome Component 7 (EXOSC7) ELISA Kit

20-abx258177
  • EUR 8853.60
  • EUR 4719.60
  • EUR 1093.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Exosome Component 1 (EXOSC1) ELISA Kit

abx387219-96tests 96 tests
EUR 1093.2

Human Exosome Component 3 (EXOSC3) ELISA Kit

abx387222-96tests 96 tests
EUR 1093.2

Human Exosome Component 4 (EXOSC4) ELISA Kit

abx387223-96tests 96 tests
EUR 1093.2

Human Exosome Component 5 (EXOSC5) ELISA Kit

abx387224-96tests 96 tests
EUR 1093.2

Human Exosome Component 8 (EXOSC8) ELISA Kit

abx387226-96tests 96 tests
EUR 1093.2

Human Exosome Component 9 (EXOSC9) ELISA Kit

abx387227-96tests 96 tests
EUR 1093.2

Human Exosome Component 1 (EXOSC1)ELISA Kit

201-12-2688 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Exosome Component 2 (EXOSC2)ELISA Kit

201-12-2689 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Exosome Component 3 (EXOSC3)ELISA Kit

201-12-2690 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Exosome Component 4 (EXOSC4)ELISA Kit

201-12-2691 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Exosome Component 1 (EXOSC5)ELISA Kit

201-12-2692 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Exosome Component 2 (EXOSC2) ELISA Kit

DLR-EXOSC2-Hu-48T 48T
EUR 620.4
Description: A sandwich quantitative ELISA assay kit for detection of Human Exosome Component 2 (EXOSC2) in samples from tissue homogenates, cell lysates or other biological fluids.

Human Exosome Component 2 (EXOSC2) ELISA Kit

DLR-EXOSC2-Hu-96T 96T
EUR 807.6
Description: A sandwich quantitative ELISA assay kit for detection of Human Exosome Component 2 (EXOSC2) in samples from tissue homogenates, cell lysates or other biological fluids.

Human Exosome Component 7 (EXOSC7) ELISA Kit

SEM477Hu-10x96wellstestplate 10x96-wells test plate
EUR 6227.58
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Exosome Component 7 (EXOSC7) in Tissue homogenates, cell lysates and other biological fluids.

Human Exosome Component 7 (EXOSC7) ELISA Kit

SEM477Hu-1x48wellstestplate 1x48-wells test plate
EUR 618.04
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Exosome Component 7 (EXOSC7) in Tissue homogenates, cell lysates and other biological fluids.

Human Exosome Component 7 (EXOSC7) ELISA Kit

SEM477Hu-1x96wellstestplate 1x96-wells test plate
EUR 831.48
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Exosome Component 7 (EXOSC7) in Tissue homogenates, cell lysates and other biological fluids.

Human Exosome Component 7 (EXOSC7) ELISA Kit

SEM477Hu-5x96wellstestplate 5x96-wells test plate
EUR 3381.66
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Exosome Component 7 (EXOSC7) in Tissue homogenates, cell lysates and other biological fluids.

Human Exosome Component 7 (EXOSC7) ELISA Kit

4-SEM477Hu
  • EUR 6288.00
  • EUR 3322.80
  • EUR 831.60
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Exosome Component 7 (EXOSC7) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

Human Exosome Component 2 ELISA Kit (EXOSC2)

RK01330 96 Tests
EUR 625.2

Human Exosome Component 7 ELISA Kit (EXOSC7)

RK01331 96 Tests
EUR 625.2

Human Exosome Component 2 (EXOSC2) ELISA Kit

RDR-EXOSC2-Hu-48Tests 48 Tests
EUR 652.8

Human Exosome Component 2 (EXOSC2) ELISA Kit

RDR-EXOSC2-Hu-96Tests 96 Tests
EUR 907.2

Human Exosome Component 1(EXOSC5)ELISA Kit

QY-E01127 96T
EUR 433.2

Human Exosome Component 4(EXOSC4)ELISA Kit

QY-E01128 96T
EUR 433.2

Human Exosome Component 3(EXOSC3)ELISA Kit

QY-E01129 96T
EUR 433.2

Human Exosome Component 2(EXOSC2)ELISA Kit

QY-E01130 96T
EUR 433.2

Human Exosome Component 1(EXOSC1)ELISA Kit

QY-E01131 96T
EUR 433.2

Human Exosome Component 2 (EXOSC2) ELISA Kit

RD-EXOSC2-Hu-48Tests 48 Tests
EUR 625.2

Human Exosome Component 2 (EXOSC2) ELISA Kit

RD-EXOSC2-Hu-96Tests 96 Tests
EUR 867.6

Human Exosome Component 2 (EXOSC2) ELISA Kit

RDR-EXOSC2-Hu-48T 48T
EUR 475.34
Description: tissue homogenates, cell lysates and other biological fluids.

Human Exosome Component 2 (EXOSC2) ELISA Kit

RDR-EXOSC2-Hu-96T 96T
EUR 679.04
Description: tissue homogenates, cell lysates and other biological fluids.

Human Exosome Component 2 (EXOSC2) ELISA Kit

DL-EXOSC2-Hu 96T
EUR 441
Description: tissue homogenates, cell lysates or other biological fluids.

Human Exosome Component 2 (EXOSC2) ELISA Kit

DLR-EXOSC2-Hu 96T
EUR 463
Description: tissue homogenates, cell lysates or other biological fluids.

Human Exosome Component 1 (EXOSC1)ELISA Kit

YLA3328HU-48T 48T
EUR 435

Human Exosome Component 1 (EXOSC1)ELISA Kit

YLA3328HU-96T 96T
EUR 562.5

Here, we employed programming field frit-inlet fractionation asymmetrical flow field-flow (FIAF4) coupled with light scattering multiangle (MALS) for effective separation of exosomes from the protein bound to the free and lipoproteins present in serum samples using the methods of pre-treatment is different, namely , a commercial kit exosome isolation, ultracentrifugation (UC), and a simple centrifugation followed by ultrafiltration (UF) .Sizes of exosomes eluted, as calculated by the MALS signal, approximated well with the results of dynamic light scattering batch of fraction was collected and the particle size of polystyrene