Comparison of methods and characterization of small RNAs from plasma extracellular vesicles of HIV/HCV coinfected patients

The hepatitis C virus (HCV) and human immunodeficiency virus (HIV) hijacks the host machine exosomal addition to the mechanism of infection and evasion of the immune system, modifying a small RNA (smRNA) cargo during infection. Our surface epitope tagged extracellular vesicles (EV) on plasma HIV / HCV-coinfected patients and their cargo smRNA profile, by comparing the different isolation procedures.

six EVS isolation procedures were compared: ultracentrifugation, and five polyethylene glycol based on a different method (commercial, combined with step purification column and two custom); and the two RNA commercial kit (phenol and non-phenol based) are used. High-throughput sequencing of smRNAs do.

Exosomal surface epitopes were analyzed by MACSPlex Exosome Kit. Four miRNAs shown the difference between protocol (HSA-miR-205-5p and HSA-let-7a / b / f-5p). Selection of RNA isolation kits impact on the detection of miRNAs and other smRNAs, where the phenol-based RNA isolation kits are made welcome. EVS enriched surface with HLA-DR / DP / DQ, CD81, and CD8. There are three liver-specific miRNAs expressed (let-7a-5p, miR-21-5p and HSA-miR-122-5p), thus, EVS cargo may reflect the evolution of liver disease.

Another smRNAs like piwi-interacting RNAs were also detected for the first time. Custom-based polyethylene glycol precipitation method combined with phenol-based RNA kit produces a higher number of smRNAs for EVs isolated from patients with HIV / HCV plasma.


micrographs was used to determine the crystal structure, western blotting was used to identify markers exosomal, and NanoSight used to characterize the nanoparticles. The relative expression levels of miRNAs which former is calculated using quantitative PCR and compared between patients with PC and CP. The expression levels of the two former miRNA-21 and miRNA-210 were significantly higher in patients with PC compared to patients with CP (both P <0.001).

However, no significant differences in serum levels are relatively free miR-21 and miR-210 were observed between the 2 groups of patients (both P> 0.05). ex-miRNA-21 and miRNA-210 was associated with tumor stage, as well as other factors. Ex diagnostic potential miRNA-21 and miRNA-210 levels were 83 and 85%, respectively. In addition, when the former Mirna and serum carbohydrate antigen 19-9 combined expression levels, accuracy is increased to 90%. This study identified that the serum former miRNAs, miRNA-21 and miRNA-210 may be of value as a potential biomarker and therapeutic target for the diagnosis and treatment of PC.

Comparison of methods and characterization of small RNAs from plasma extracellular vesicles of HIV/HCV coinfected patients
Comparison of methods and characterization of small RNAs from plasma extracellular vesicles of HIV/HCV coinfected patients

Evaluation exosome separation of human serum with fractional asymmetric flow field-flow-inlet frit and multiangle light scattering

Exosomes are extracellular vesicles that mediate communication between, the immune response, and tumor metastasis. However, the isolation of blood exosome complex because their size and density similar to blood lipoprotein.

ExoQuick Exosome Isolation and RNA Purification kit (for Tissue Culture Media)
EQ806TC-1 10 preps
EUR 493

ExoQuick-LP for lipoprotein pre-clear & exosome isolation
EXOLP5A-1 5 reactions
EUR 473

ExoQuick ULTRA EV Isolation Kit for Serum and Plasma
EQULTRA-20A-1 20 reactions
EUR 941

ExoQuick-CG cGMP-grade Exosome Isolation reagent (50 ml)
EXOCG50A-1 50 ml
EUR 1106

SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns)
RA800TC-1 10 reactions
EUR 595

ExoQuick-TC PLUS Exosome Purification Kit - for tissue culture media
EQPL10TC-1 10 reactions
EUR 518

ExoQualiTM Exosome Isolation Kit (Plasma)
CEIK-004L-10T 10 T Ask for price

ExoQualiTM Exosome Isolation Kit (Plasma)
CEIK-004L-30T 30 T Ask for price

Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800TC-1 with 50ml ExoQuick-TC, RA805A-1 and RA812A-1 components)
RA822TC-1 20 profiles
EUR 1533

ExoQualiTM Exosome Isolation Kit (Plasma & Serum)
CEIK-006L-10T 10 T Ask for price

ExoQualiTM Exosome Isolation Kit (Plasma & Serum)
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ExoQualiTM Exosome DNA Extraction Kit (Plasma & Serum)
CEIK-048L-30T 30T Ask for price

ExoQualiTM Exosome DNA Extraction Kit (Plasma & Serum)
CEIK-048L-50T 50T Ask for price

ExoQualiTM Exosome RNA Extraction Kit (Plasma & Serum)
CEIK-093L-30T 30T Ask for price

ExoQualiTM Exosome RNA Extraction Kit (Plasma & Serum)
CEIK-093L-50T 50T Ask for price

EXO-Prep for exosome isolation from Plasma and serum
HBM-EXP-B5 5 ml
EUR 181.44

ExoQualiTM Total Exosome Capture & Quantification Kit (Plasma)
CEIK-108L-96wells 96 wells Ask for price

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Plasma, Luminometric)
K1206-100 each
EUR 1161.6

Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL)
TMEXO-1 100 reactions
EUR 522

ExoQuick TC 10ml
EXOTC10A-1 10 ml
EUR 341

ExoQuick TC 50ml
EXOTC50A-1 50 ml
EUR 1147

ExoQuali™ Lyophilized Exosome Standards ( Human Plasma )
CEIK-068L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Plasma )
CEIK-068L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQuant? Overall Exosome Capture and Quantification ELISA Assay Kit (Human Plasma, Colorimetric)
K1201-100 each
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ExoQualiTM Overall Exosome Isolation Reagent (from plasma)
CEIK-045L-30T 30 T Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from plasma)
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ExoQualiTM Human Exosome Capture and Isolation Kits (for Serum/Plasma) (CD9 Capture Beads)
CEIK-101L-10T 10T Ask for price

ExoQualiTM Human Exosome Capture and Isolation Kits (for Serum/Plasma) (CD63 Capture Beads)
CEIK-102L-10T 10T Ask for price

ExoQualiTM Overall Exosome Isolation Immunoplates (from Plasma)
CEIK-095L-96wells 96 wells Ask for price

ExoQualiTM Human Exosome Capture and Isolation Kits (for Serum/Plasma) (CD9/CD63 Capture Beads)
CEIK-103L-10T 10T Ask for price

Exosome Purification and Detection Kit (Plasma)
abx290026-25tests 25 tests
EUR 777.6

Exosome Isolation kit (for serum & plasma -Exosome-RNA-NGS)
P101NGS - Ask for price

XCF COMPLETE Exosome and cfDNA Isolation Kit (for Serum and Plasma)
XCF100A-1 20 rxn
EUR 583

Plasma/Serum Exosome Purification and RNA Isolation Mini Kit
58300 50 Preps
EUR 627

Plasma/Serum Exosome Purification and RNA Isolation Midi Kit
58500 25 Preps
EUR 569.4

Plasma/Serum Exosome Purification and RNA Isolation Maxi Kit
58600 15 Preps
EUR 341.4

Plasma/Serum Exosome Purification Kit
57440 4 Preps
EUR 12

Exosome Isolation kit (for serum & plasma)
P101 10 rxn
EUR 199

Exosome Isolation kit (for serum & plasma)
P101L 40 rxn
EUR 769

Exosome Isolation kit (for serum & plasma)
P101S 2 rxn
EUR 69

Exosome precipitation from Plasma + Thrombin
EPStep-T 5 ml
EUR 291.5
Description: Exosome precipitation from Plasma + Thrombin - 5ml

Plasma/Serum Exosome and Free-Circulating RNA Isolation Mini Kit
59500 50 Preps
EUR 797.4

Plasma/Serum Exosome and Free-Circulating RNA Isolation Midi Kit
59600 25 Preps
EUR 683.4

Plasma/Serum Exosome and Free-Circulating RNA Isolation Maxi Kit
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EUR 513

Plasma/Serum Exosome Purification Mini Kit
57400 50 Preps
EUR 473.4

Plasma/Serum Exosome Purification Midi Kit
57500 25 Preps
EUR 441

Plasma/Serum Exosome Purification Maxi Kit
57600 15 Preps
EUR 395.4

ExoQuick-TC ULTRA EV Isolation Kit for Tissue Culture Media
EQULTRA-20TC-1 20 reactions
EUR 518

EXTRAClean Plasma/Serum Exosome Purification and RNA Isolation Mini Kit
73100 50 Preps
EUR 783

EXTRAClean Plasma/Serum Exosome Purification and RNA Isolation Midi Kit
73110 25 Preps
EUR 711

EXTRAClean Plasma/Serum Exosome Purification and RNA Isolation Maxi Kit
73120 15 Preps
EUR 426.6

ExoStd? Plasma Fluorescent Exosome Standard
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EUR 907.2

Exosome Precipitation Solution from Plasma - Serum
EPStep-PS 5 ml
EUR 209
Description: Exosome Precipitation Solution from Plasma and Serum - 5ml

EXTRAClean Plasma/Serum Exosome and Free-Circulating RNA Isolation Mini Kit
73400 50 Preps
EUR 996.6

EXTRAClean Plasma/Serum Exosome and Free-Circulating RNA Isolation Midi Kit
73410 25 Preps
EUR 853.8

EXTRAClean Plasma/Serum Exosome and Free-Circulating RNA Isolation Maxi Kit
73420 15 Preps
EUR 641.4

Lyophilized Exosome Standard (Human Plasma)
ESL-25 30 µgx2 vials
EUR 1604.4
Description: Lyophilized exosomes can be used as control standards for FACS, WB, ELISA and as calibration standards for quantitation of exosome-derived markers from biological samples.

Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome)
P101EZ - Ask for price

ExoTEST™ for Overall Exosome capture and quantification from human plasma
HBM-RTK-POF/TP KIT
EUR 506.52

VEX Exosome Isolation Reagent (from plasma)
R603 10 ml
EUR 769.2

ExoDNAPS? Circulating and Exosome-associated DNA Extraction Kit (Human Plasma/Serum, 20 reactions)
K1230-20 each
EUR 861.6

ExoDNAPS? Circulating and Exosome-associated DNA Extraction Kit (Human Plasma/Serum, 40 reactions)
K1230-40 each
EUR 1273.2

Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome) sample
P101EZ--sample - Ask for price

Exosomal RNA Isolation Kit (for use with Urine Exosome Purification Kits or Plasma/Serum Exosome Purification Kits)
58000 50 Preps
EUR 243

Exosome derived from human plasma (healthy donors)
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EUR 1147.5
Description: Exosome

ExoQualiTM Exosome DNA Extraction Kit (Pre-Isolated Exosomes)
CEIK-050L-30T 30T Ask for price

ExoQualiTM Exosome DNA Extraction Kit (Pre-Isolated Exosomes)
CEIK-050L-50T 50T Ask for price

ExoQualiTM Exosome RNA Extraction Kit (Pre-Isolated Exosomes)
CEIK-051L-30T 30T Ask for price

ExoQualiTM Exosome RNA Extraction Kit (Pre-Isolated Exosomes)
CEIK-051L-50T 50T Ask for price

ExoTEST™ for Tumor-derived Exosome enrichment and quantification from human plasma
HBM-RTK-PTF/TC KIT
EUR 506.52

ExoQualiTM Exosome Isolation Kit (Urine)
CEIK-003L-10T 10 T Ask for price

ExoQualiTM Exosome Isolation Kit (Urine)
CEIK-003L-30T 30 T Ask for price

ExoQualiTM Exosome Isolation Kit (Serum)
CEIK-005L-10T 10 T Ask for price

ExoQualiTM Exosome Isolation Kit (Serum)
CEIK-005L-30T 30 T Ask for price

ExoStd? Lyophilized Exosome Standard (30 µg, Human Plasma, 2 vials)
M1040-2 each
EUR 711.6

ExoStd? Lyophilized Exosome Standard (30 µg, Human Plasma, 4 vials)
M1040-4 each
EUR 1090.8

ExoStd? Lyophilized Exosome Standard (30 µg, Human Plasma, 6 vials)
M1040-6 each
EUR 1560

ExoStd? Lyophilized Exosome Standard (100 µg, Human Plasma, 2 vials)
M1041-2 each
EUR 853.2

ExoStd? Lyophilized Exosome Standard (100 µg, Human Plasma, 4 vials)
M1041-4 each
EUR 1364.4

ExoStd? Lyophilized Exosome Standard (100 µg, Human Plasma, 6 vials)
M1041-6 each
EUR 1886.4

ExoQualiTM Exosome Isolation Kit (Cell Media)
CEIK-001L-10T 10 T Ask for price

ExoQualiTM Exosome Isolation Kit (Cell Media)
CEIK-001L-30T 30 T Ask for price

ExoQualiTM Exosome Isolation Kit (All in one)
CEIK-008L-10T 10 T Ask for price

ExoQualiTM Exosome Isolation Kit (All in one)
CEIK-008L-30T 30 T Ask for price

ExoFACS? Kit for Plasma Exosomes
K1232-20 each
EUR 952.8

TumorExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay)
M1016-100-T each
EUR 874.8

TumorExoPure? Immunoplates (Exosome Isolation, plasma, luminometric assay)
M1017-100-W each
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TumorExoPure? Immunoplates (Exosome Isolation, plasma, fluorimetric assay)
M1018-100-B each
EUR 888

GlialExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay)
M1022-100-T each
EUR 888

GlialExoPure? Immunoplates (Exosome Isolation, plasma, luminometric assay)
M1023-100-W each
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GlialExoPure? Immunoplates (Exosome Isolation, plasma, fluorimetric assay)
M1024-100-B each
EUR 888

NeuralExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay)
M1019-100-T each
EUR 907.2

NeuralExoPure? Immunoplates (Exosome Isolation, plasma, luminometric assay)
M1020-100-W each
EUR 888

NeuralExoPure? Immunoplates (Exosome Isolation, plasma, fluorimetric assay)
M1021-100-B each
EUR 888

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Cell media, Colorimetric)
K1205-100 each
EUR 1161.6

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Cell Media, Luminometric)
K1210-100 each
EUR 1161.6

ExoQualiTM Exosome Isolation Kit (Stem Cell Media)
CEIK-002L-10T 10 T Ask for price

ExoQualiTM Exosome Isolation Kit (Stem Cell Media)
CEIK-002L-30T 30 T Ask for price

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Urine, Colorimetric)
K1202-100 each
EUR 1161.6

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Serum, Colorimetric)
K1203-100 each
EUR 1161.6

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Urine, Luminometric)
K1207-100 each
EUR 1161.6

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Human Serum, Luminometric)
K1208-100 each
EUR 1161.6

ExoQualiTM Exosome Isolation Kit (other body fluids)
CEIK-007L-10T 10 T Ask for price

ExoQualiTM Exosome Isolation Kit (other body fluids)
CEIK-007L-30T 30 T Ask for price

ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum)
M1038-10 each
EUR 1005.6

ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum)
M1038-20 each
EUR 1546.8

ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum)
M1038-3 each
EUR 523.2

ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum)
M1038-5 each
EUR 796.8

Fluorescent labeled exosome derived from human plasma (healthy donors)
Exo-F488-Plasma Each
EUR 1593.75
Description: Exosome

Fluorescent labeled Exosome derived from human plasma (healthy donors)
Exo-F665 Each
EUR 1249.5
Description: Exosome

ExoPure? Immunoplates (Overall Exosome Isolation, plasma, urine, colorimetric assay)
M1004-100-T each
EUR 1136.4

ExoPure? Immunoplates (Overall Exosome Isolation, plasma, urine, luminometric assay)
M1005-100-W each
EUR 888

ExoPure? Immunoplates (Overall Exosome Isolation, plasma, urine, fluorometric assay)
M1006-100-B each
EUR 888

ExoQualiTM Exosome Chromatography Columns
ESEC-001L-5columns 5 columns Ask for price

Monocytes/PlateletExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay)
M1025-100-T each
EUR 888

Monocytes/PlateletExoPure? Immunoplates (Exosome Isolation, plasma, luminometric assay)
M1026-100-W each
EUR 888

Monocytes/PlateletExoPure? Immunoplates (Exosome Isolation, plasma, fluorimetric assay)
M1027-100-B each
EUR 888

ExoQualiTM Total Exosome Capture & Quantification Kit (Serum)
CEIK-107L-96wells 96 wells Ask for price

ExoQualiTM Total Exosome Capture & Quantification Kit (Urine)
CEIK-110L-96wells 96 wells Ask for price

ExoQualiTM Max Exosome Chromatography Columns
ESEC-002L-3columns 3 columns Ask for price

ExoQualiTM Mini Exosome Chromatography Columns
ESEC-003L-2columns 2 columns Ask for price

ExoQualiTM Exosome DNA Extraction Kit (Urine & Cell Supernatant)
CEIK-049L-10T 10T Ask for price

ExoQualiTM Exosome DNA Extraction Kit (Urine & Cell Supernatant)
CEIK-049L-20T 20T Ask for price

ExoQualiTM Exosome RNA Extraction Kit (Urine & Cell Supernatant)
CEIK-094L-10T 10T Ask for price

ExoQualiTM Exosome RNA Extraction Kit (Urine & Cell Supernatant)
CEIK-094L-20T 20T Ask for price

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum, 10 reactions)
M1039-10 each
EUR 1005.6

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum, 20 reactions)
M1039-20 each
EUR 1546.8

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum, 3 reactions)
M1039-3 each
EUR 523.2

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum, 5 reactions)
M1039-5 each
EUR 796.8

Human Neural derived plasma exosomes ELISA Kit
QY-E05473 96T
EUR 400

ExoQualiTM Total Exosome Capture & Quantification Kit (Cell Supernatant)
CEIK-109L-96wells 96 wells Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Serum )
CEIK-070L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Serum )
CEIK-070L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Urine )
CEIK-071L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Urine )
CEIK-071L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQualiTM Human Exosome Capture and Isolation Kits (for Cell Culture Media/ Urine) (CD9 Capture Beads)
CEIK-097L-10T 10T Ask for price

ExoQualiTM Human Exosome Capture and Isolation Kits (for Cell Culture Media/ Urine) (CD63 Capture Beads)
CEIK-098L-10T 10T Ask for price

ExoQualiTM Human Exosome Capture and Isolation Kits (for Cell Culture Media/ Urine) (CD81 Capture Beads)
CEIK-099L-10T 10T Ask for price

ExoQualiTM Mouse Exosome Capture and Isolation Kits (for Cell Culture Media/ Urine) (mCD9 Capture Beads)
CEIK-104L-10T 10T Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Saliva )
CEIK-069L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Saliva )
CEIK-069L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

Saliva Exosome Collection and Preservation Kit
65400 50 Devices
EUR 407.4

ExoELISA-Step for detection and quantification of exosomes from human serum/plasma
Exo2508 96
EUR 654.5
Description: ExoELISA-Step for detection and quantification of exosomes from human serum/plasma

ExoQualiTM Mouse Exosome Capture and Isolation Kits (for Cell Culture Media/ Urine) (mCD63 Capture Beads)
CEIK-105L-10T 10T Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from serum)
CEIK-044L-30T 30 T Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from serum)
CEIK-044L-50T 50 T Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from urine)
CEIK-046L-10T 10 T Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from urine)
CEIK-046L-24T 24 T Ask for price

ExoQuali™ Lyophilized Exosome Standards ( MM1 cell line )
CEIK-064L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( MM1 cell line )
CEIK-064L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( PC3 cell line )
CEIK-065L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( PC3 cell line )
CEIK-065L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

Exosome Labeling and Cleaning Kit
68400 25 Preps
EUR 185.4

Exosome Isolation Kit for Cell Culture Supernatant,Serum,Plasma,Breast Milk,Urine,Saliva,Yeast, Plant
CSB-EI0102 2T
EUR 340
Description: Exosomes are a type of extracellular vesicles secreted by cells that contain a variety of RNA, proteins, and phospholipids with a diameter of about 30-150 nm. Exosomes play an important role in the transfer of material and information between cells.This exosome isolation kit provides a simple and reliable method to extract intact exosomes from the cell supernatant. Comparing with other well-known brand kits, cell supernatant exosome isolation kit provided by CUSABIO has its own features. It is suitable for the isolation of exosomes from the supernatant of a variety of cells. And the exosomes isolated by this kit are comparable to ultracentrifugation with High Purity and High Yield. Moreover, this kit is simple to use and no equipment requirement.Exosomal vesicles isolated by this product are suitable for various downstream applications, such as electron microscopy analysis, NTA analysis, NanoFCM analysis, Western Blot, fluorescence quantitative (qPCR), and high-throughput sequencing, etc.

Here, we employed programming field frit-inlet fractionation asymmetrical flow field-flow (FIAF4) coupled with light scattering multiangle (MALS) for effective separation of exosomes from the protein bound to the free and lipoproteins present in serum samples using the methods of pre-treatment is different, namely , a commercial kit exosome isolation, ultracentrifugation (UC), and a simple centrifugation followed by ultrafiltration (UF) .Sizes of exosomes eluted, as calculated by the MALS signal, approximated well with the results of dynamic light scattering batch of fraction was collected and the particle size of polystyrene