miR-365 (microRNA): Potential Biomarker in Oral Squamous Cell Carcinoma Exosomes and Extracellular Vesicles

Introduction: miR-365 is a non-coding microRNA that regulates transcription and have been shown to promote metastasis in oncogenes and some types of cancer, while suppressing the effect on others. Many microRNAs is produced and then exported extracellular in exosomes, the small extracellular vesicles ranging from 30 to 100 nm are found in eukaryotic fluid and facilitate various cellular functions.

Exosomes and extracellular vesicles produced by many types of cells, including cancer cells mouth-though no study to date has evaluated miR-365 and oral cancer exosomes or extracellular vesicles. Based on this information, the question of our research is to evaluate whether the cancer of the mouth produces extracellular exosomes or vesicles containing miR-365.


Materials and methods: Two commercially available oral cancer cell lines (SCC25 and CAL27) and normal oral keratinocytes (OKF4) were grown in serum-free media, supplemented with exosome-depleted fetal bovine serum. extracellular vesicles and exosomes then isolated using Invitrogen Total exosome RNA and protein isolation kit for processing using HSA-miR-365a-5p microRNA qPCR assay kits.


Results: RNA was isolated from exosome-depleted supernatant from each cell line-SCC9, SCC15, SCC25, and CAL27 (oral squamous cell carcinoma) and OKF4 (oral epithelial cell lines). the relative concentration of RNA were similar among each cell line, which is not significant, p = 0.233. RNA quality was established by A260: A280 absorbance using a NanoDrop, expressing the purity ranging from 1.73 to 1.86. Expression of miR-16 microRNA is used to confirm the presence of exosomes extracted and extracellular vesicles. The presence of miR-365 was later confirmed and normalized to miR-16 expression, which indicates the rate of increase of miR-365 in both CAL27 and SCC25. In addition, the normalization relative quantity (RQ) for miR-365 exhibited a greater variation between SCC25 (1.382 to 4.363) of cells CAL27 (1.248 to 1.536).


Conclusion: These results confirm that miR-365 was not only expressed in cancer cell lines of the mouth, but also subsequently exported to exosomes and extracellular vesicles derived from this culture. This data can help to contextualize this microRNA potential to contribute to the phenotype and behavior of oral cancers that express this microRNA. Future research will begin to investigate the potential mechanisms and pathways and to determine whether miR-365 may be useful as a biomarker of cancer of the mouth for saliva or fluid biopsy.

miR-365 (microRNA): Potential Biomarker in Oral Squamous Cell Carcinoma Exosomes and Extracellular Vesicles
miR-365 (microRNA): Potential Biomarker in Oral Squamous Cell Carcinoma Exosomes and Extracellular Vesicles

Exosomal Circulating microRNAs as potential novel biomarker detection in pancreatic cancer

Circulating microRNAs exosomal (ex-miRNA) is reflective of the characteristics of the tumor and valuable biomarkers in a variety of tumor types. In addition, miRNAs serve an important role in tumor progression and metastasis.

This study aims to determine the outstanding ex-miRNA-21 and miRNA-210 as a new biomarker for patients with pancreatic cancer (PC). For this purpose, the former serum miRNAs extracted from the serum of patients with PC (n = 30) and chronic pancreatitis (CP) (n = 10) using the RNA isolation kit.

Exosome Precipitation Solution from Plasma - Serum
EPStep-PS 5 ml
EUR 209
Description: Exosome Precipitation Solution from Plasma and Serum - 5ml

Lentivirus Precipitation Solution
VC100 100 ml
EUR 194.04

Lentivirus Precipitation Solution
VC125 250 ml
EUR 432.6

Lentivirus Precipitation Solution
VC150 500 ml
EUR 814.8

Retrovirus Precipitation Solution
VC200 100 ml
EUR 194.04

Retrovirus Precipitation Solution
VC225 250 ml
EUR 432.6

Retrovirus Precipitation Solution
VC250 500 ml
EUR 814.8

Exosome precipitation from Plasma + Thrombin
EPStep-T 5 ml
EUR 291.5
Description: Exosome precipitation from Plasma + Thrombin - 5ml

Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL)
TMEXO-1 100 reactions
EUR 522

PEG-it Virus Precipitation Solution (100 mL aliquot)
LV810A-1 100 ml
EUR 325

PEG-it Virus Precipitation Solution (250 mL aliquot)
LV825A-1 250 ml
EUR 711

TMB for Precipitation
42R-TB100x 1000 ml
EUR 473
Description: High sensitivity TMB Substrate for Precipitation assays

TMB for Precipitation
MBS538888-1L 1L
EUR 620

TMB for Precipitation
MBS538888-5x1L 5x1L
EUR 2700

DNA Precipitation Buffer
D106 55 ml
EUR 210
Description: DNA Precipitation Buffer by Cygnus Technologies is available in Europe via Gentaur.

PEG Virus Precipitation Kit
K904-200 each
EUR 1201.2

PEG Virus Precipitation Kit
K904-50 each
EUR 451.2

PEG Virus Precipitation Kit
K2190-200 200 preparations
EUR 1270.8

PEG Virus Precipitation Kit
K2190-50 50 preparations
EUR 418
Description: High Viral Titer, No ultracentrifugation

ExoQuick Exosome RNA Column Purification kit
EQ808A-1 20 preps
EUR 344

Chicken IgY precipitation reagent
85R-1010 4 ml
EUR 638
Description: Chicken IgY precipitation reagent for use in immunoassays

Chicken IgY precipitation reagent
MBS5307794-2mL 2mL
EUR 975

Chicken IgY precipitation reagent
MBS5307794-5x2mL 5x2mL
EUR 4245

In Vivo SDS-K+ Precipitation Kit
TG1011-1 100 assays
EUR 362.4

In Vivo SDS-K+ Precipitation Kit
TG1011-2 250 assays
EUR 496.8

Nucleic Acid Precipitation Enhancer
TBS6010 2 x0.5 mL
EUR 98

ExoQuick-LP for lipoprotein pre-clear & exosome isolation
EXOLP5A-1 5 reactions
EUR 473

In Vitro SDS-K+ Precipitation Kit
TG1010-1 100 assays
EUR 550.8

In Vitro SDS-K+ Precipitation Kit
TG1010-2 250 assays
EUR 685.2

50ML Protein Precipitation Kit - 50ML
NAT1254 50ML
EUR 348.3

PEG Virus Precipitation Kit - 200 preparations
GWB-AXR384 200 preparations Ask for price

PEG Virus Precipitation Kit - 50 preparations
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101Bio High Efficiency Protein Precipitation kit
P5W6 30 ml / 30 ml
EUR 129

Kinesis TELOS PPT Protein Precipitation Plate - EACH
CHR6509 EACH
EUR 118.97

LPA (Linear Polyacrylamide) for DNA/RNA Precipitation
10510000-1 10 mg
EUR 85.98

LPA (Linear Polyacrylamide) for DNA/RNA Precipitation
10510000-2 25 mg
EUR 140.95

Minute TM High Efficiency Protein Precipitation kit
WA-006 each
EUR 160

Kinesis TELOS PPT Protein Precipitation Columns - PK100
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EUR 249.75

ExoQuick-CG cGMP-grade Exosome Isolation reagent (50 ml)
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EUR 1106

ExoQuick PLUS Exosome Purification Kit - for serum and plasma
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EUR 518

MinuteTM Native Protein Precipitation Kit from Saliva (20 tests)
SV-043 each
EUR 330

ExoQuick Exosome Isolation and RNA Purification kit (for Serum and Plasma)
EQ806A-1 20 preps
EUR 493

ExoQuick Exosome Isolation and RNA Purification kit (for Tissue Culture Media)
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EUR 493

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EQPL10TC-1 10 reactions
EUR 518

SeraMir Exosome RNA Purification kit (5ml ExoQuick and 20 exoRNA columns)
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EUR 493

SeraMir Exosome RNA Purification kit (10ml ExoQuick-TC and 10 exoRNA columns) 10 preps
RA806TC-1 10 preps
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SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns)
RA800TC-1 10 reactions
EUR 595

Exo-Check Exosome Antibody Array
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EUR 469.2

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Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome) sample
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ExoQuick TC 10ml
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EUR 341

ExoQuick TC 50ml
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EUR 1147

Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome) sample
P100EZ-sample - Ask for price

Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800TC-1 with 50ml ExoQuick-TC, RA805A-1 and RA812A-1 components)
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EUR 1533

SDS-Blue™ - Coomassie based solution for protein staining in SDS-PAGE
04-GSB
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TMB Precipitating (High Sensitivity)
TM5125 125 ml
EUR 28.28

TMB Precipitating (High Sensitivity)
TM5500 500 ml
EUR 70.71

TMB Precipitating (High Sensitivity)
TM5999 1000 ml
EUR 127.28

TMB Precipitating Reagent (For Membranes)
TMP125 125 ml
EUR 96

TMB Precipitating Reagent (For Membranes)
TMP500 500 ml
EUR 159.6

TMB Precipitating Reagent (For Membranes)
TMP999 1000 ml
EUR 236.4

TMB Precipitating (Standard Sensitivity)
TM3125 125 ml
EUR 24.43

TMB Precipitating (Standard Sensitivity)
TM3500 500 ml
EUR 64.28

TMB Precipitating (Standard Sensitivity)
TM3999 1000 ml
EUR 117

Universal Protein Precipitating Reagent
BSP012 100preps
EUR 97.58

ExoQuick ULTRA EV Isolation Kit for Serum and Plasma
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EUR 941

ExoQuick-TC ULTRA EV Isolation Kit for Tissue Culture Media
EQULTRA-20TC-1 20 reactions
EUR 518

Sulfur Precipitated pure, 98%
69181 500 Gms
EUR 1.73
Description: Part B

Silicic acid, precipitated
GRM622-500G 1 unit
EUR 13.55
Description: Silicic acid, precipitated

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RM1316-500G 1 unit
EUR 11.59
Description: Sulphur precipitated, Hi-AR™

ExoQualiTM Exosome DNA Extraction Kit (Pre-Isolated Exosomes)
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ExoQualiTM Exosome DNA Extraction Kit (Pre-Isolated Exosomes)
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ExoQualiTM Exosome RNA Extraction Kit (Pre-Isolated Exosomes)
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ExoQualiTM Exosome RNA Extraction Kit (Pre-Isolated Exosomes)
CEIK-051L-50T 50T Ask for price

Calcium resinate (precipitated)
C01800 25G
EUR 132.08

Magnesium stearate precipitated fine pow
GRM7219-500G 1 unit
EUR 6.93
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Rabbit plasma, barium chloride precipitate
MBS136153-500mL 500mL
EUR 1360

Rabbit plasma, barium chloride precipitate
MBS136153-50mL 50mL
EUR 270

Rabbit plasma, barium chloride precipitate
MBS136153-5x500mL 5x500mL
EUR 5930

ExoQualiTM Exosome Isolation Kit (Urine)
CEIK-003L-10T 10 T Ask for price

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ExoQualiTM Exosome Isolation Kit (Serum)
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Calcium carbonate, 99%, precipitated, Ph. Eur., BP, USP, FCC grade
GX0868 1kg
EUR 30.35

Calcium carbonate, 99%, precipitated, Ph. Eur., BP, USP, FCC grade
GX0868-1 1
EUR 33.1

ExoQualiTM Exosome Isolation Kit (Plasma)
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ExoQualiTM Exosome Isolation Kit (Plasma)
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ExoQualiTM Exosome Chromatography Columns
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ExoQualiTM Exosome Isolation Kit (All in one)
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ExoQualiTM Max Exosome Chromatography Columns
ESEC-002L-3columns 3 columns Ask for price

ExoQualiTM Exosome Isolation Kit (Plasma & Serum)
CEIK-006L-10T 10 T Ask for price

ExoQualiTM Exosome Isolation Kit (Plasma & Serum)
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ExoQualiTM Mini Exosome Chromatography Columns
ESEC-003L-2columns 2 columns Ask for price

ExoQualiTM Exosome Isolation Kit (Stem Cell Media)
CEIK-002L-10T 10 T Ask for price

ExoQualiTM Exosome Isolation Kit (Stem Cell Media)
CEIK-002L-30T 30 T Ask for price

ExoQualiTM Exosome Isolation Kit (other body fluids)
CEIK-007L-10T 10 T Ask for price

ExoQualiTM Exosome Isolation Kit (other body fluids)
CEIK-007L-30T 30 T Ask for price

ExoQualiTM Exosome DNA Extraction Kit (Plasma & Serum)
CEIK-048L-30T 30T Ask for price

ExoQualiTM Exosome DNA Extraction Kit (Plasma & Serum)
CEIK-048L-50T 50T Ask for price

ExoQualiTM Exosome RNA Extraction Kit (Plasma & Serum)
CEIK-093L-30T 30T Ask for price

ExoQualiTM Exosome RNA Extraction Kit (Plasma & Serum)
CEIK-093L-50T 50T Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Serum )
CEIK-070L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Serum )
CEIK-070L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Urine )
CEIK-071L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Urine )
CEIK-071L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Plasma )
CEIK-068L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Plasma )
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ExoQuali™ Lyophilized Exosome Standards ( Human Saliva )
CEIK-069L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Saliva )
CEIK-069L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from serum)
CEIK-044L-30T 30 T Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from serum)
CEIK-044L-50T 50 T Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from urine)
CEIK-046L-10T 10 T Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from urine)
CEIK-046L-24T 24 T Ask for price

ExoQuali™ Lyophilized Exosome Standards ( MM1 cell line )
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ExoQuali™ Lyophilized Exosome Standards ( MM1 cell line )
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ExoQuali™ Lyophilized Exosome Standards ( PC3 cell line )
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ExoQualiTM Overall Exosome Isolation Reagent (from plasma)
CEIK-045L-30T 30 T Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from plasma)
CEIK-045L-50T 50 T Ask for price

ExoQuali™ Lyophilized Exosome Standards ( A549 cell line )
CEIK-054L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( A549 cell line )
CEIK-054L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQualiTM Total Exosome Capture & Quantification Kit (Serum)
CEIK-107L-96wells 96 wells Ask for price

ExoQualiTM Total Exosome Capture & Quantification Kit (Urine)
CEIK-110L-96wells 96 wells Ask for price

ExoQuali™ Lyophilized Exosome Standards ( COLO1 cell line )
CEIK-058L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( COLO1 cell line )
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ExoQualiTM Total Exosome Capture & Quantification Kit (Plasma)
CEIK-108L-96wells 96 wells Ask for price

ExoQuali™ Lyophilized Exosome Standards ( U87 MG cell line )
CEIK-067L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( U87 MG cell line )
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ExoQuali™ Lyophilized Exosome Standards ( B16F10 cell line )
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ExoQuali™ Lyophilized Exosome Standards ( B16F10 cell line )
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ExoQuali™ Lyophilized Exosome Standards ( BLCL21 cell line )
CEIK-056L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( BLCL21 cell line )
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ExoQuali™ Lyophilized Exosome Standards ( HCT116 cell line )
CEIK-060L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( HCT116 cell line )
CEIK-060L-5vials1x1010particlespervial 5 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( BPH-1 cell line )
CEIK-057L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( BPH-1 cell line )
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ExoQuali™ Lyophilized Exosome Standards ( K-562 cell line )
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ExoQuali™ Lyophilized Exosome Standards ( K-562 cell line )
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ExoQualiTM Overall Exosome Isolation Immunoplates (from Serum)
CEIK-023L-96wells 96 wells Ask for price

ExoQualiTM Overall Exosome Isolation Immunoplates (from Saliva)
CEIK-026L-96wells 96 wells Ask for price

ExoQualiTM Overall Exosome Isolation Immunoplates (from Plasma)
CEIK-095L-96wells 96 wells Ask for price

ExoQualiTM Overall Exosome Isolation Immunoplates (from Urine)
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ExoQualiTM Exosome DNA Extraction Kit (Urine & Cell Supernatant)
CEIK-049L-10T 10T Ask for price

ExoQualiTM Exosome DNA Extraction Kit (Urine & Cell Supernatant)
CEIK-049L-20T 20T Ask for price

ExoQualiTM Exosome RNA Extraction Kit (Urine & Cell Supernatant)
CEIK-094L-10T 10T Ask for price

ExoQualiTM Exosome RNA Extraction Kit (Urine & Cell Supernatant)
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*Innovative Grade US Origin Rabbit Barium Chloride Precipitate Plasma Lyophilized
IRBBACL2PPLALY500ML each
EUR 1045
Description: *Innovative Grade US Origin Rabbit Barium Chloride Precipitate Plasma Lyophilized

*Innovative Grade US Origin Rabbit Barium Chloride Precipitate Plasma Lyophilized
IRBBACL2PPLALY50ML each
EUR 150
Description: *Innovative Grade US Origin Rabbit Barium Chloride Precipitate Plasma Lyophilized

ExoQuali™ Lyophilized Exosome Standards ( SK-N-SH cell line )
CEIK-066L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

To exosome identified in serum, transmission electron micrographs were used to determine the crystal structure, western blotting was used to identify markers exosomal, and NanoSight used to characterize the nanoparticles.