miR-365 (microRNA): Potential Biomarker in Oral Squamous Cell Carcinoma Exosomes and Extracellular Vesicles

Introduction: miR-365 is a non-coding microRNA that regulates transcription and have been shown to promote metastasis in oncogenes and some types of cancer, while suppressing the effect on others. Many microRNAs is produced and then exported extracellular in exosomes, the small extracellular vesicles ranging from 30 to 100 nm are found in eukaryotic fluid and facilitate various cellular functions.

Exosomes and extracellular vesicles produced by many types of cells, including cancer cells mouth-though no study to date has evaluated miR-365 and oral cancer exosomes or extracellular vesicles. Based on this information, the question of our research is to evaluate whether the cancer of the mouth produces extracellular exosomes or vesicles containing miR-365.


Materials and methods: Two commercially available oral cancer cell lines (SCC25 and CAL27) and normal oral keratinocytes (OKF4) were grown in serum-free media, supplemented with exosome-depleted fetal bovine serum. extracellular vesicles and exosomes then isolated using Invitrogen Total exosome RNA and protein isolation kit for processing using HSA-miR-365a-5p microRNA qPCR assay kits.


Results: RNA was isolated from exosome-depleted supernatant from each cell line-SCC9, SCC15, SCC25, and CAL27 (oral squamous cell carcinoma) and OKF4 (oral epithelial cell lines). the relative concentration of RNA were similar among each cell line, which is not significant, p = 0.233. RNA quality was established by A260: A280 absorbance using a NanoDrop, expressing the purity ranging from 1.73 to 1.86. Expression of miR-16 microRNA is used to confirm the presence of exosomes extracted and extracellular vesicles. The presence of miR-365 was later confirmed and normalized to miR-16 expression, which indicates the rate of increase of miR-365 in both CAL27 and SCC25. In addition, the normalization relative quantity (RQ) for miR-365 exhibited a greater variation between SCC25 (1.382 to 4.363) of cells CAL27 (1.248 to 1.536).


Conclusion: These results confirm that miR-365 was not only expressed in cancer cell lines of the mouth, but also subsequently exported to exosomes and extracellular vesicles derived from this culture. This data can help to contextualize this microRNA potential to contribute to the phenotype and behavior of oral cancers that express this microRNA. Future research will begin to investigate the potential mechanisms and pathways and to determine whether miR-365 may be useful as a biomarker of cancer of the mouth for saliva or fluid biopsy.

 miR-365 (microRNA): Potential Biomarker in Oral Squamous Cell Carcinoma Exosomes and Extracellular Vesicles
miR-365 (microRNA): Potential Biomarker in Oral Squamous Cell Carcinoma Exosomes and Extracellular Vesicles

Exosomal Circulating microRNAs as potential novel biomarker detection in pancreatic cancer

Circulating microRNAs exosomal (ex-miRNA) is reflective of the characteristics of the tumor and valuable biomarkers in a variety of tumor types. In addition, miRNAs serve an important role in tumor progression and metastasis.

This study aims to determine the outstanding ex-miRNA-21 and miRNA-210 as a new biomarker for patients with pancreatic cancer (PC). For this purpose, the former serum miRNAs extracted from the serum of patients with PC (n = 30) and chronic pancreatitis (CP) (n = 10) using the RNA isolation kit.

Exosome precipitation from Plasma + Thrombin

EPStep-T 5 ml
EUR 336

ExoQuick Exosome RNA Column Purification kit

EQ808A-1 20 preps
EUR 402

Exosome precipitation from Plasma and Serum

EPStep-PS 5 ml
EUR 297

ExoQuick-LP for lipoprotein pre-clear & exosome isolation

EXOLP5A-1 5 reactions
EUR 533

ExoQuick PLUS Exosome Purification Kit - for serum and plasma

EQPL10A-1 10 reactions
EUR 579

ExoQuick-CG cGMP-grade Exosome Isolation reagent (50 ml)

EXOCG50A-1 50 ml
EUR 1181

PEG-it Virus Precipitation Solution (100 mL aliquot)

LV810A-1 100 ml
EUR 382

PEG-it Virus Precipitation Solution (250 mL aliquot)

LV825A-1 250 ml
EUR 777

ExoQuick-TC PLUS Exosome Purification Kit - for tissue culture media

EQPL10TC-1 10 reactions
EUR 579

SDS-Blue™ - Coomassie based solution for protein staining in SDS-PAGE

04-GSB
  • EUR 120.00
  • EUR 80.00
  • 1L
  • 500mL
Description: SDS-Blue™ is an innovative patented formula, based on Coomassie blue, that comes in a convenient ready to use format for staining proteins in SDS-PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis). The formulation of SDS-Blue™ provides numerous advantages compared to the classic Coomassie staining or to other similar protein stains. SDS-Blue™ provides higher sensitivity, virtually no background and eliminates the need for destaining of the gel due to its high specificity and affinity to bind to protein only. Not only does SDS-Blue™ yield clear and sharp bands, but it also contains no methanol and acetic acid, making it non-hazardous, safe to handle and friendly to the environment when disposed of. Two other advantages that make SDS-Blue™ the better option is that it is not light sensitive and can be stored at ambient temperature for 24 months. And this provides a considerable convenience, especially to laboratories that need and keep big amount of protein staining solutions – no more jammed refrigerators, you can keep SDS-Blue™ wherever it is most convenient for You!

ExoQuick Exosome Isolation and RNA Purification kit (for Serum and Plasma)

EQ806A-1 20 preps
EUR 554

ExoQuick Exosome Isolation and RNA Purification kit (for Tissue Culture Media)

EQ806TC-1 10 preps
EUR 554

SeraMir Exosome RNA Purification kit (5ml ExoQuick and 20 exoRNA columns)

RA806A-1 20 preps
EUR 554

Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL)

TMEXO-1 100 reactions
EUR 583

Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome)

P101EZ - Ask for price

ExoQuick TC 10ml

EXOTC10A-1 10 ml
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42R-TB100x 1000 ml
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Description: High sensitivity TMB Substrate for Precipitation assays

DNA Precipitation Buffer

D106 55 ml
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Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome)

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Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome) sample

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Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome) sample

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SeraMir Exosome RNA Purification kit (10ml ExoQuick-TC and 10 exoRNA columns) 10 preps

RA806TC-1 10 preps
EUR 554

Chicken IgY precipitation reagent

85R-1010 4 ml
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PEG Virus Precipitation Kit

K904-200
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PEG Virus Precipitation Kit

K904-50
EUR 376

50ML Protein Precipitation Kit

NAT1254 50ML
EUR 225

PEG Virus Precipitation Kit

K2190-200 200 preparations
EUR 1059

PEG Virus Precipitation Kit

K2190-50 50 preparations
EUR 405

SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns)

RA800TC-1 10 reactions
EUR 659

Psoriasis Exosome

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101Bio High Efficiency Protein Precipitation kit

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In Vitro SDS-K+ Precipitation Kit

TG1010-1 100 assays
EUR 459

In Vitro SDS-K+ Precipitation Kit

TG1010-2 250 assays
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In Vivo SDS-K+ Precipitation Kit

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In Vivo SDS-K+ Precipitation Kit

TG1011-2 250 assays
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Leukemia - ALL Exosome

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Bladder Cancer Exosome

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Thyroid Cancer Exosome

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Psoriasis Exosome RNA

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Lyophilized Exosome Standards

ExoA375 100 μg
EUR 303.5

Lyophilized Exosome Standards

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Lyophilized Exosome Standards

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Lyophilized Exosome Standards

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Lyophilized Exosome Standards

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Lyophilized Exosome Standards

ExoUrine 100 μg
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Sodium Pyruvate Solution, 100 mM Solution

CCM1753-100 100 mL
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SDS Solution

2102-100
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EDTA Solution

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Prehybridization Solution

K2191050-1 5 ml
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Hybridization Solution

K2191050-2 5 ml
EUR 157
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Blocking Solution

K2191050-8 250 ul
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Stop Solution

F006 12 ml
EUR 180
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Denaturing solution

DE540 1L
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TRIMMING SOLUTION

99-676-CM 1 L /pk
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Description: Specialty Media; Islet products

DTT Solution

R-1104 1 ml, 500 mM
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AC001 20 ml
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Borax Solution

BOR999 1000 ml
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OAA125 125 ml
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Orcein Solution

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EUR 109

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OAA999 1000 ml
EUR 148

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MET125 125 ml
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EUR 95

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MET999 1000 ml
EUR 132

Schiff's Solution

SRF030 30 ml
EUR 64

Schiff's Solution

SRF250 250 ml
EUR 77

Schiff's Solution

SRF3800 1 Gal.
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Schiff's Solution

SRF500 500 ml
EUR 103

Schiff's Solution

SRF999 1000 ml
EUR 149

Tartrazine Solution

TZQ125 125 ml
EUR 64

Tartrazine Solution

TZQ500 500 ml
EUR 98

Tartrazine Solution

TZQ999 1000 ml
EUR 127

50ml TC Tubes, Conical, 440 units/box

04-5540150 440 units/box
EUR 71

GMP IL4, 50µg

04-GMP-HU-IL4-50UG 50µg
EUR 483
Description: Recombinant Human IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15000 Dalton. The rHuIL-4 is purified by proprietary chromatographic techniques.

rHu IL 2 , 3MIU

04-RHIL2-02F01 1 vial
EUR 249
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated.

rHu IL 2 , 3MIU , Lot 200908F02

04-RHIL2-08F02 1 vial
EUR 249
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated.

PRE-GMP rHu GM-CSF, Molgramostim-Leukoma

04-RHUGM-CSF-7A10 300 µg
EUR 385
Description: Recombinant human GM-CSF produced in E.coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids, two pairs of disulfide bonds and having a molecular mass of approximately 14.5kD.

Fluorescent Exosome Standard (Urine )

ESF-15 100 µg
EUR 1723
Description: Fluorescent exosomes are labeled with green dye, which offer a stable fluorescent labeling.

Exosome Component 5 Protein

20-abx260873
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 25 ug
  • 5 ug

Exosome Component 7 Protein

20-abx261347
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug

Exosome Component 10 Protein

20-abx261805
  • EUR 4365.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug

Exosome Component 4 Protein

20-abx261954
  • EUR 4490.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug

Exosome Component 9 Protein

20-abx262123
  • EUR 328.00
  • EUR 5089.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg

Exosome Component 8 Protein

20-abx262850
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg

Exosome Component 3 Protein

20-abx263422
  • EUR 230.00
  • EUR 1609.00
  • EUR 328.00
  • 1 µg
  • 50 ug
  • 5 ug

Exosome Component 1 Protein

20-abx263423
  • EUR 1609.00
  • EUR 328.00
  • EUR 230.00
  • 100 ug
  • 10 ug
  • 2 µg

Exosome-TEM-easy Kit

P130 - Ask for price

Normal human exosome sample

P140 - Ask for price

Diabetes (Type 1) Exosome

P141-DB1 - Ask for price

Diabetes (Type 2) Exosome

P141-DB2 - Ask for price

Leukemia - Hairy Cell Exosome

P141-HC - Ask for price

Leukemia - Multiple Myeloma Exosome

P141-MM - Ask for price

Melanoma (skin cancer) Exosome

P141-MN - Ask for price

Non-Hodgkin lymphoma Exosome

P141-NH - Ask for price

Systemic Lupus Erythematosus Exosome

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Uterine Cervical Cancer Exosome

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Uterine Corpus Cancer Exosome

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Normal human exosome RNA

P240 - Ask for price

Leukemia - ALL Exosome RNA

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Leukemia - AML Exosome RNA

P241-AML - Ask for price

Bladder Cancer Exosome RNA

P241-BL - Ask for price

Breast Cancer Exosome RNA

P241-BR - Ask for price

Leukemia - CLL Exosome RNA

P241-CLL - Ask for price

Leukemia - CML Exosome RNA

P241-CML - Ask for price

Colorectal Cancer Exosome RNA

P241-CO - Ask for price

Crohn's Disease Exosome RNA

P241-CR - Ask for price

Kidney Cancer Exosome RNA

P241-KN - Ask for price

Lung Cancer Exosome RNA

P241-LG - Ask for price

Liver Cancer Exosome RNA

P241-LV - Ask for price

Nasopharyngeal Cancer Exosome RNA

P241-NA - Ask for price

Prostate Cancer Exosome RNA

P241-PR - Ask for price

Rheumatoid Arthritis Exosome RNA

P241-RA - Ask for price

Thyroid Cancer Exosome RNA

P241-TH - Ask for price

EXOCET Exosome Quantitation Kit

EXOCET96A-1 96 reactions
EUR 521

FluoroCet Exosome Quantitation Kit

FCET96A-1 96 reactions
EUR 726

Gentamicin Solution, Gentamicin Solution, 50 mg/mL

CCM1123-010 10 mL
EUR 64.05

ExoQuick ULTRA EV Isolation Kit for Serum and Plasma

EQULTRA-20A-1 20 reactions
EUR 579

ExoFectin Plasmid DNA-into-Exosome Kit (DNA transfection kit for exosome)

P402 - Ask for price

Stop/Elution Solution

85R-123 250 ml
EUR 155
Description: Elution or stop reagent for use in immunoassays

RNase A Solution

G118 1.0 ml; 10 mg/ml
EUR 102

Tyloxapol (10% ) solution

B1159-250
EUR 512

Tyloxapol (10% ) solution

B1159-50
EUR 175

Stop Solution, 25ML

X020-25ML 25ML
EUR 109

Stop Solution, 5ML

X020-5ML 5ML
EUR 65

Stop Solution, 1ML

X046-1ML 1ML
EUR 65

Creatinine Solution, 100ML

X116-100ML 100ML
EUR 131

Creatinine Solution, 25ML

X120-25ML 25ML
EUR 173

Extraction Solution, 250ML

X123-250ML 250ML
EUR 263

Extraction Solution, 50ML

X123-50ML 50ML
EUR 123

Mesotocin Solution, 625UL

X127-625UL 625UL
EUR 227

Isotocin Solution, 625UL

X128-625UL 625UL
EUR 227

Stop Solution, 5ML

X134-5ML 5ML
EUR 65

FastHyb-Hybridization Solution

L1031250 250 ml
EUR 249
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

DNA Storage Solution

LB3401010 10 ml
EUR 79
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Southgate's Mucicarmine Solution

M1430-125
EUR 327

Wright-Giemsa Solution

M1438-1000
EUR 316

Wright-Giemsa Solution

M1438-500
EUR 207

To exosome identified in serum, transmission electron micrographs were used to determine the crystal structure, western blotting was used to identify markers exosomal, and NanoSight used to characterize the nanoparticles.