miR-365 (microRNA): Potential Biomarker in Oral Squamous Cell Carcinoma Exosomes and Extracellular Vesicles

Introduction: miR-365 is a non-coding microRNA that regulates transcription and have been shown to promote metastasis in oncogenes and some types of cancer, while suppressing the effect on others. Many microRNAs is produced and then exported extracellular in exosomes, the small extracellular vesicles ranging from 30 to 100 nm are found in eukaryotic fluid and facilitate various cellular functions.

Exosomes and extracellular vesicles produced by many types of cells, including cancer cells mouth-though no study to date has evaluated miR-365 and oral cancer exosomes or extracellular vesicles. Based on this information, the question of our research is to evaluate whether the cancer of the mouth produces extracellular exosomes or vesicles containing miR-365.


Materials and methods: Two commercially available oral cancer cell lines (SCC25 and CAL27) and normal oral keratinocytes (OKF4) were grown in serum-free media, supplemented with exosome-depleted fetal bovine serum. extracellular vesicles and exosomes then isolated using Invitrogen Total exosome RNA and protein isolation kit for processing using HSA-miR-365a-5p microRNA qPCR assay kits.


Results: RNA was isolated from exosome-depleted supernatant from each cell line-SCC9, SCC15, SCC25, and CAL27 (oral squamous cell carcinoma) and OKF4 (oral epithelial cell lines). the relative concentration of RNA were similar among each cell line, which is not significant, p = 0.233. RNA quality was established by A260: A280 absorbance using a NanoDrop, expressing the purity ranging from 1.73 to 1.86. Expression of miR-16 microRNA is used to confirm the presence of exosomes extracted and extracellular vesicles. The presence of miR-365 was later confirmed and normalized to miR-16 expression, which indicates the rate of increase of miR-365 in both CAL27 and SCC25. In addition, the normalization relative quantity (RQ) for miR-365 exhibited a greater variation between SCC25 (1.382 to 4.363) of cells CAL27 (1.248 to 1.536).


Conclusion: These results confirm that miR-365 was not only expressed in cancer cell lines of the mouth, but also subsequently exported to exosomes and extracellular vesicles derived from this culture. This data can help to contextualize this microRNA potential to contribute to the phenotype and behavior of oral cancers that express this microRNA. Future research will begin to investigate the potential mechanisms and pathways and to determine whether miR-365 may be useful as a biomarker of cancer of the mouth for saliva or fluid biopsy.

 miR-365 (microRNA): Potential Biomarker in Oral Squamous Cell Carcinoma Exosomes and Extracellular Vesicles
miR-365 (microRNA): Potential Biomarker in Oral Squamous Cell Carcinoma Exosomes and Extracellular Vesicles

Exosomal Circulating microRNAs as potential novel biomarker detection in pancreatic cancer

Circulating microRNAs exosomal (ex-miRNA) is reflective of the characteristics of the tumor and valuable biomarkers in a variety of tumor types. In addition, miRNAs serve an important role in tumor progression and metastasis.

This study aims to determine the outstanding ex-miRNA-21 and miRNA-210 as a new biomarker for patients with pancreatic cancer (PC). For this purpose, the former serum miRNAs extracted from the serum of patients with PC (n = 30) and chronic pancreatitis (CP) (n = 10) using the RNA isolation kit.

Exosome precipitation from Plasma + Thrombin

EPStep-T 5 ml
EUR 403.2

ExoQuick Exosome RNA Column Purification kit

EQ808A-1 20 preps
EUR 482.4

Exosome precipitation from Plasma and Serum

EPStep-PS 5 ml
EUR 356.4

Exo-Check Exosome Antibody Array

322-EXO-FBS-50A-1 100 µg
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ExoQuick-LP for lipoprotein pre-clear & exosome isolation

EXOLP5A-1 5 reactions
EUR 639.6

ExoQuick-CG cGMP-grade Exosome Isolation reagent (50 ml)

EXOCG50A-1 50 ml
EUR 1417.2

ExoQuick PLUS Exosome Purification Kit - for serum and plasma

EQPL10A-1 10 reactions
EUR 694.8

PEG-it Virus Precipitation Solution (250 mL aliquot)

LV825A-1 250 ml
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PEG-it Virus Precipitation Solution (100 mL aliquot)

LV810A-1 100 ml
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ExoQuick-TC PLUS Exosome Purification Kit - for tissue culture media

EQPL10TC-1 10 reactions
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ExoQuick Exosome Isolation and RNA Purification kit (for Serum and Plasma)

EQ806A-1 20 preps
EUR 664.8

ExoQuick Exosome Isolation and RNA Purification kit (for Tissue Culture Media)

EQ806TC-1 10 preps
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SeraMir Exosome RNA Purification kit (5ml ExoQuick and 20 exoRNA columns)

RA806A-1 20 preps
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Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL)

TMEXO-1 100 reactions
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ExoQuick TC 10ml

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EXOTC50A-1 50 ml
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SDS-Blue™ - Coomassie based solution for protein staining in SDS-PAGE

04-GSB
  • EUR 144.00
  • EUR 96.00
  • 1L
  • 500mL
Description: SDS-Blue™ is an innovative patented formula, based on Coomassie blue, that comes in a convenient ready to use format for staining proteins in SDS-PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis). The formulation of SDS-Blue™ provides numerous advantages compared to the classic Coomassie staining or to other similar protein stains. SDS-Blue™ provides higher sensitivity, virtually no background and eliminates the need for destaining of the gel due to its high specificity and affinity to bind to protein only. Not only does SDS-Blue™ yield clear and sharp bands, but it also contains no methanol and acetic acid, making it non-hazardous, safe to handle and friendly to the environment when disposed of. Two other advantages that make SDS-Blue™ the better option is that it is not light sensitive and can be stored at ambient temperature for 24 months. And this provides a considerable convenience, especially to laboratories that need and keep big amount of protein staining solutions – no more jammed refrigerators, you can keep SDS-Blue™ wherever it is most convenient for You!

Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome)

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DNA Precipitation Buffer

D106 55 ml
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TMB for Precipitation

42R-TB100x 1000 ml
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Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome) sample

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SeraMir Exosome RNA Purification kit (10ml ExoQuick-TC and 10 exoRNA columns) 10 preps

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Chicken IgY precipitation reagent

85R-1010 4 ml
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Description: Chicken IgY precipitation reagent for use in immunoassays

PEG Virus Precipitation Kit

K904-200
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PEG Virus Precipitation Kit

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PEG Virus Precipitation Kit

K2190-200 200 preparations
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PEG Virus Precipitation Kit

K2190-50 50 preparations
EUR 486

50ML Protein Precipitation Kit

NAT1254 50ML
EUR 237.12

SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns)

RA800TC-1 10 reactions
EUR 790.8

Psoriasis Exosome

P141-PS - Ask for price

101Bio High Efficiency Protein Precipitation kit

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In Vitro SDS-K+ Precipitation Kit

TG1010-1 100 assays
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In Vitro SDS-K+ Precipitation Kit

TG1010-2 250 assays
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In Vivo SDS-K+ Precipitation Kit

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Kinesis TELOS PPT Protein Precipitation Columns

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Kinesis TELOS PPT Protein Precipitation Plate

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Solution

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Lyophilized Exosome Standards

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EUR 364.2

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Description: This product includes 40 ml of Solution #11 for large-scale preparative extraction of membrane proteins (up to 130 mg) from the cell membranes.

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Description: This product includes 40 ml of Solution #12 for large-scale preparative extraction of membrane proteins (up to 130 mg) from the cell membranes.

Denaturing solution

DE540 1L
EUR 73.57

Borax Solution

BOR015 15 ml
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BOR125 125 ml
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BOR999 1000 ml
EUR 219.6

Stop Solution

F006 12 ml
EUR 216
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SDS Solution

2102-100
EUR 164.4

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TRIMMING SOLUTION

99-676-CM 1 L /pk
EUR 105.6
Description: Specialty Media; Islet products

Orcein Solution

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EUR 79.2

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Plug Solution

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EUR 102

Prehybridization Solution

K2191050-1 5 ml
EUR 164.4
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Hybridization Solution

K2191050-2 5 ml
EUR 188.4
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Blocking Solution

K2191050-8 250 ul
EUR 164.4
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Schiff's Solution

SRF030 30 ml
EUR 76.8

Schiff's Solution

SRF250 250 ml
EUR 92.4

Schiff's Solution

SRF3800 1 Gal.
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Schiff's Solution

SRF500 500 ml
EUR 123.6

Schiff's Solution

SRF999 1000 ml
EUR 178.8

Tartrazine Solution

TZQ125 125 ml
EUR 76.8

Tartrazine Solution

TZQ500 500 ml
EUR 117.6

Tartrazine Solution

TZQ999 1000 ml
EUR 152.4

DTT Solution 

R-1104-1 1 ml, 500 mM
EUR 48.4

Chloroprene Solution

S-930A 1ML
EUR 128.82

Fortification Solution

5242-F 1ML
EUR 36.48

Fortification Solution

5252-FS 1ML
EUR 42.18

Decalcifying Solution

DECAL01 100ML
EUR 31.92

TISAB Solution

TISAB1L-500ML 500ML
EUR 33.06

TISAB Solution

TISAB5 5L
EUR 191.52

DTT Solution 

R-1104
  • EUR 131.34
  • EUR 48.40
  • 1 ml, 500 mM
  • 1 ml, 500 mM

Bovine Anti-Chicken IgY Beads for immuno-precipitation

XG-6134 1 mL
EUR 546.72
Description: The anti-IgY beads were made by cross-linking of bovine IgG anti-IgY antibodies to Protein A/G agarose beads. IgY is the original designation of chicken IgG like immunoglobulin.

ExoQuick ULTRA EV Isolation Kit for Serum and Plasma

EQULTRA-20A-1 20 reactions
EUR 694.8

FluoroCet Exosome Quantitation Kit

FCET96A-1 96 reactions
EUR 871.2

Fluorescent Exosome Standard (Urine )

ESF-15 100 µg
EUR 2067.6
Description: Fluorescent exosomes are labeled with green dye, which offer a stable fluorescent labeling.

EXOCET Exosome Quantitation Kit

EXOCET96A-1 96 reactions
EUR 625.2

Exosome Component 9 Peptide

46-967P 0.1 mg
EUR 405.6
Description: (CT) PMSCL1 Peptide

Exosome Component 5 Protein

20-abx260873
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 25 ug
  • 5 ug

Exosome Component 7 Protein

20-abx261347
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

Exosome Component 10 Protein

20-abx261805
  • EUR 5238.00
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

Exosome Component 4 Protein

20-abx261954
  • EUR 5388.00
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

Exosome Component 9 Protein

20-abx262123
  • EUR 393.60
  • EUR 6106.80
  • EUR 276.00
  • 10 ug
  • 1 mg
  • 2 µg

Exosome Component 8 Protein

20-abx262850
  • EUR 393.60
  • EUR 7676.40
  • EUR 276.00
  • 10 ug
  • 1 mg
  • 2 µg

Exosome Component 3 Protein

20-abx263422
  • EUR 276.00
  • EUR 1930.80
  • EUR 393.60
  • 1 µg
  • 50 ug
  • 5 ug

Exosome Component 1 Protein

20-abx263423
  • EUR 1930.80
  • EUR 393.60
  • EUR 276.00
  • 100 ug
  • 10 ug
  • 2 µg

Exosome-TEM-easy Kit

P130 - Ask for price

Diabetes (Type 1) Exosome

P141-DB1 - Ask for price

Diabetes (Type 2) Exosome

P141-DB2 - Ask for price

Leukemia - Hairy Cell Exosome

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Leukemia - Multiple Myeloma Exosome

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Melanoma (skin cancer) Exosome

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Non-Hodgkin lymphoma Exosome

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Systemic Lupus Erythematosus Exosome

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Uterine Cervical Cancer Exosome

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Uterine Corpus Cancer Exosome

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Normal human exosome RNA

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Colorectal Cancer Exosome RNA

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Gentamicin Solution, Gentamicin Solution, 50 mg/mL

CCM1123-010 10 mL
EUR 76.86

ExoFectin Plasmid DNA-into-Exosome Kit (DNA transfection kit for exosome)

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To exosome identified in serum, transmission electron micrographs were used to determine the crystal structure, western blotting was used to identify markers exosomal, and NanoSight used to characterize the nanoparticles.