miR-365 (microRNA): Potential Biomarker in Oral Squamous Cell Carcinoma Exosomes and Extracellular Vesicles

Introduction: miR-365 is a non-coding microRNA that regulates transcription and have been shown to promote metastasis in oncogenes and some types of cancer, while suppressing the effect on others. Many microRNAs is produced and then exported extracellular in exosomes, the small extracellular vesicles ranging from 30 to 100 nm are found in eukaryotic fluid and facilitate various cellular functions.

Exosomes and extracellular vesicles produced by many types of cells, including cancer cells mouth-though no study to date has evaluated miR-365 and oral cancer exosomes or extracellular vesicles. Based on this information, the question of our research is to evaluate whether the cancer of the mouth produces extracellular exosomes or vesicles containing miR-365.


Materials and methods: Two commercially available oral cancer cell lines (SCC25 and CAL27) and normal oral keratinocytes (OKF4) were grown in serum-free media, supplemented with exosome-depleted fetal bovine serum. extracellular vesicles and exosomes then isolated using Invitrogen Total exosome RNA and protein isolation kit for processing using HSA-miR-365a-5p microRNA qPCR assay kits.


Results: RNA was isolated from exosome-depleted supernatant from each cell line-SCC9, SCC15, SCC25, and CAL27 (oral squamous cell carcinoma) and OKF4 (oral epithelial cell lines). the relative concentration of RNA were similar among each cell line, which is not significant, p = 0.233. RNA quality was established by A260: A280 absorbance using a NanoDrop, expressing the purity ranging from 1.73 to 1.86. Expression of miR-16 microRNA is used to confirm the presence of exosomes extracted and extracellular vesicles. The presence of miR-365 was later confirmed and normalized to miR-16 expression, which indicates the rate of increase of miR-365 in both CAL27 and SCC25. In addition, the normalization relative quantity (RQ) for miR-365 exhibited a greater variation between SCC25 (1.382 to 4.363) of cells CAL27 (1.248 to 1.536).


Conclusion: These results confirm that miR-365 was not only expressed in cancer cell lines of the mouth, but also subsequently exported to exosomes and extracellular vesicles derived from this culture. This data can help to contextualize this microRNA potential to contribute to the phenotype and behavior of oral cancers that express this microRNA. Future research will begin to investigate the potential mechanisms and pathways and to determine whether miR-365 may be useful as a biomarker of cancer of the mouth for saliva or fluid biopsy.

miR-365 (microRNA): Potential Biomarker in Oral Squamous Cell Carcinoma Exosomes and Extracellular Vesicles
miR-365 (microRNA): Potential Biomarker in Oral Squamous Cell Carcinoma Exosomes and Extracellular Vesicles

Exosomal Circulating microRNAs as potential novel biomarker detection in pancreatic cancer

Circulating microRNAs exosomal (ex-miRNA) is reflective of the characteristics of the tumor and valuable biomarkers in a variety of tumor types. In addition, miRNAs serve an important role in tumor progression and metastasis.

This study aims to determine the outstanding ex-miRNA-21 and miRNA-210 as a new biomarker for patients with pancreatic cancer (PC). For this purpose, the former serum miRNAs extracted from the serum of patients with PC (n = 30) and chronic pancreatitis (CP) (n = 10) using the RNA isolation kit.

Exosome Precipitation Solution from Plasma - Serum
EPStep-PS 5 ml
EUR 209
Description: Exosome Precipitation Solution from Plasma and Serum - 5ml

Exosome precipitation from Plasma + Thrombin
EPStep-T 5 ml
EUR 291.5
Description: Exosome precipitation from Plasma + Thrombin - 5ml

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VC125 250 ml
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Lentivirus Precipitation Solution
VC150 500 ml
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Retrovirus Precipitation Solution
VC200 100 ml
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Retrovirus Precipitation Solution
VC225 250 ml
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Retrovirus Precipitation Solution
VC250 500 ml
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Lentivirus Precipitation Solution
VC100 100 ml
EUR 194.04

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EUR 522

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PEG-it Virus Precipitation Solution (100 mL aliquot)
LV810A-1 100 ml
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42R-TB100x 1000 ml
EUR 473
Description: High sensitivity TMB Substrate for Precipitation assays

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D106 55 ml
EUR 210
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EUR 344

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EUR 473

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85R-1010 4 ml
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Description: Chicken IgY precipitation reagent for use in immunoassays

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EUR 362.4

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EUR 348.3

PEG Virus Precipitation Kit - 200 preparations
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101Bio High Efficiency Protein Precipitation kit
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Kinesis TELOS PPT Protein Precipitation Plate - EACH
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EUR 160

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ExoQuick Exosome Isolation and RNA Purification kit (for Serum and Plasma)
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MinuteTM Native Protein Precipitation Kit from Saliva (20 tests)
SV-043 each
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EUR 595

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SDS-Blue™ - Coomassie based solution for protein staining in SDS-PAGE
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TM5125 125 ml
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TMB Precipitating Reagent (For Membranes)
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TMB Precipitating Reagent (For Membranes)
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TMB Precipitating (Standard Sensitivity)
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TMB Precipitating (Standard Sensitivity)
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EUR 117

Universal Protein Precipitating Reagent
BSP012 100preps
EUR 97.58

ExoQuick ULTRA EV Isolation Kit for Serum and Plasma
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EUR 941

ExoQuick-TC ULTRA EV Isolation Kit for Tissue Culture Media
EQULTRA-20TC-1 20 reactions
EUR 518

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GRM622-500G 1 unit
EUR 13.55
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ExoQualiTM Exosome Isolation Kit (Plasma)
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ExoQualiTM Exosome Chromatography Columns
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Calcium resinate (precipitated)
C01800 25G
EUR 132.08

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ExoQualiTM Mini Exosome Chromatography Columns
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Magnesium stearate precipitated fine pow
GRM7219-500G 1 unit
EUR 6.93
Description: Magnesium stearate precipitated fine pow

ExoQualiTM Exosome Isolation Kit (other body fluids)
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ExoQualiTM Exosome DNA Extraction Kit (Plasma & Serum)
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ExoQualiTM Exosome RNA Extraction Kit (Plasma & Serum)
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ExoQualiTM Exosome RNA Extraction Kit (Plasma & Serum)
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Calcium carbonate, 99%, precipitated, Ph. Eur., BP, USP, FCC grade
GX0868-1 1
EUR 33.1

ExoQuali™ Lyophilized Exosome Standards ( Human Serum )
CEIK-070L-2vials1x1010particlespervial 2 vials (> 1x10^10 particles per vial) Ask for price

ExoQuali™ Lyophilized Exosome Standards ( Human Serum )
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ExoQuali™ Lyophilized Exosome Standards ( Human Urine )
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ExoQuali™ Lyophilized Exosome Standards ( Human Urine )
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ExoQuali™ Lyophilized Exosome Standards ( Human Saliva )
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ExoQualiTM Overall Exosome Isolation Reagent (from serum)
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ExoQualiTM Overall Exosome Isolation Reagent (from urine)
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ExoQualiTM Overall Exosome Isolation Reagent (from urine)
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ExoQuali™ Lyophilized Exosome Standards ( A549 cell line )
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ExoQualiTM Total Exosome Capture & Quantification Kit (Serum)
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ExoQualiTM Total Exosome Capture & Quantification Kit (Urine)
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ExoQuali™ Lyophilized Exosome Standards ( COLO1 cell line )
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ExoQualiTM Total Exosome Capture & Quantification Kit (Plasma)
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ExoQualiTM Overall Exosome Isolation Immunoplates (from Serum)
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ExoQualiTM Overall Exosome Isolation Immunoplates (from Plasma)
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ExoQualiTM Overall Exosome Isolation Immunoplates (from Urine)
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ExoQualiTM Exosome DNA Extraction Kit (Urine & Cell Supernatant)
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ExoQualiTM Exosome DNA Extraction Kit (Urine & Cell Supernatant)
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ExoQualiTM Exosome RNA Extraction Kit (Urine & Cell Supernatant)
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ExoQuali™ Lyophilized Exosome Standards ( SK-N-SH cell line )
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ExoQualiTM Overall Exosome Isolation Immunoplates (from Cell Media)
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ExoQualiTM Overall Exosome Isolation Reagent (from other body fluids)
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ExoQualiTM Overall Exosome Isolation Reagent (from other body fluids)
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ExoQualiTM Total Exosome Capture & Quantification Kit (Cell Supernatant)
CEIK-109L-96wells 96 wells Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from cell culture media)
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ExoQualiTM Overall Exosome Isolation Reagent (from cell culture media)
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*Innovative Grade US Origin Rabbit Barium Chloride Precipitate Plasma Lyophilized
IRBBACL2PPLALY500ML each
EUR 1045
Description: *Innovative Grade US Origin Rabbit Barium Chloride Precipitate Plasma Lyophilized

*Innovative Grade US Origin Rabbit Barium Chloride Precipitate Plasma Lyophilized
IRBBACL2PPLALY50ML each
EUR 150
Description: *Innovative Grade US Origin Rabbit Barium Chloride Precipitate Plasma Lyophilized

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Cell media, Colorimetric)
K1205-100 each
EUR 1161.6

ExoQuant? Overall Exosome Capture and Quantification Assay Kit (Cell Media, Luminometric)
K1210-100 each
EUR 1161.6

To exosome identified in serum, transmission electron micrographs were used to determine the crystal structure, western blotting was used to identify markers exosomal, and NanoSight used to characterize the nanoparticles.