HIC-5 expression was detected in osteosarkoma patients and osteosaroma cell lines by RT-PCR. Then RFP-SH-HIC-5 is transfected into the osteosarcoma cell line. The HIC-5 effect on cell viability, proliferation and apoptosis is assessed by MTT, Edu Kit and Flow Cytometry. Exosom is isolated from mg-63 cell supernatant by an exosomed insulation kit. Exosome-HIC-5 is confirmed by microscope electron transmission, particle size detection and RT-PCR.

Furthermore, the cells of the exosom-hic-5-5 are explored eligibility of cells, proliferation, and apoptosis. Furthermore, the C-IP test is used to identify the relationship between HIC-5 and SMAD4. TCF / LEF and the level of protein components of the WNT / β-Catenin signal are detected by the top luciferase test and Western blot. HIC-5 is regulated in osteosaroma tissue and cells. The decreased expression forced HIC-5 inhibits the proliferation of osteosarkoma cell lines, and induces the apoptosis of MG-63 and HOS. In Vivo, silencing HIC-5 sent the development of tumors. Furthermore, we isolate the exosom of the MG-63 supernatant, the excosom to conclude HIC-5 will regulate the proliferation and level of cell apoptosis of MG-63 and HOS. Furthermore, HIC-5 interacts with SMAD4 and regulates WNT / β-Catenin signals by reducing TCF / LEF activity.

Silencing HIC-5 inhibits proliferation and induces the apoptosis of cell osteosarcoma through deactivating the WNT / β-Catenin signal with the exosome path. Hypoxia is a characteristic of several serious diseases such as cardiovascular disorders and metabolism and cancer. A decrease at the oxygen level of the network induces hypoxic response in cells that seek to adapt to changing conditions. Failure to adapt to prolonged or severe hypoxia can trigger cell death. While some types of cells, such as neurons, are very susceptible to hypoxia, cancer cells utilize the hypoxia environment to undergo tumor growth, angiogenesis and metastasis. Hypoxic-induced processes trigger communication between complex cells and now there is an indication that the extracellular vesicle (EVS) plays a fundamental role in this process. The latest developments in EV isolation and the characterization methodology have raised awareness of the importance of EV purity in functional studies and cargo.

 

Exosomal limitations in the treatment of inflammatory diseases

Background: Despite great interest and many studies, there are currently no integrated standards that describe sequential manipulation with cells to get exosom for clinical use. The use of exosom has become an interesting alternative to cell therapy, because of the flexible properties of these biological vesicles. Enables scientists to manipulate their composition to produce the desired exosom to bring certain drugs, RNA and protein. This study aims to analyze the scientific literature on changes in the functional characteristics of exosom, depending on the manipulation method, which has the potential to contribute to the development of negative effects in the treatment of inflammatory genesis diseases. The choice of insulation method affects the set of protein markers disclosed, nucleic acid and receptors on microparticles.

Various surface receptors present on the exosome membrane can be engineered for the target of the lesion. Exocomals of healthy patients help reduce inflammation, normalize communication between cells and have antifibrotic, antioxidant and cytoprotective effects. Exosom can change the micro environment, but the micro environment can also change the composition of the exosomes.

Conclusion: Ecosomes obtained from sick patients carry markers of the characteristics of the appropriate disease. Such ecosomes can have pro-inflammatory properties, pro-fibrotic, cytotoxic, and oncogenic, and disrupt cellular cooperation. Until now, questions about doses, reactions to recurring giving, and the dose regime has not been fully resolved.

Proteomic exploration based on mass spectrometry of small urinary extracellular vesicles in vasculitis associated with ANCA compared to total urine

The vasculitis associated with the ANCA (AAV) is a rare, but potentially serious self-immune disease, if not nowadays, with increased mortality and morbidity. Finding the first activity biomarkers and the prognosis is very important. The small extracellular vesicles (EVS) isolated from the urine can be considered as a non-invasive source of biomarkers. We evaluated several protocols for the isolation of urinary electric vehicles.

To eliminate contaminating non-vesicular proteins due to AAV associated proteinuria, we used the processing of proteinase K. We have studied the differences in proteomes of small patient events with AAV compared to healthy controls by the freedom of LC-MS / MS labels. In parallel, we performed a similar proteomic analysis of urine samples from identical patients. The results of the study showed significant differences and similarities in the EV proteome and urine, the latter being strongly affected by proteinuria. Using bioinformatic tools, we have explored the modified proteins differently and their related routes by focusing on AAV’s pathophysiology. Our results indicate a significant regulation of Golgi enzymes, such as MAN1A1, which may be involved in the activation of the T cell by N-glycans glycosylation and can play a key role in the pathogenesis and diagnosis of AAV.

Total RNA Isolation Kit II
FATRS--001	Favorgen	100 preps	EUR 369.6

Total RNA Isolation Kit II
FATRS--050	Favorgen	50 preps	EUR 259.2

Total RNA Isolation Kit (Tissue)
P-9102	EpiGentek	50 samples	EUR 416.7

Total RNA Isolation Kit (Plant)
P-9104	EpiGentek	50 samples	EUR 416.7

AccuBlue Broad Range RNA Quantitation Kit (1000 assays)
31073	Biotium	1kit	EUR 435.6
Description: Minimum order quantity: 1 unit of 1kit

AccuBlue Broad Range RNA Quantitation Kit (200 assays)
31073-T	Biotium	1kit	EUR 169.2
Description: Minimum order quantity: 1 unit of 1kit

EpiQuik Total RNA Isolation Fast Kit
P-9105-050	EpiGentek	50 samples	EUR 176

FastPure Cell/Tissue Total RNA Isolation Kit
RC101	Vazyme	50 rxn	EUR 274.8

AccuBlue Broad Range dsDNA Quantitation Kit
31007	Biotium	1KIT	EUR 374.4
Description: Minimum order quantity: 1 unit of 1KIT

Rna Isolation Kit
11828665001	Scientific Laboratory Supplies	EACH	EUR 330.6

FastPure Plant Total RNA Isolation Kit (Polysaccharides/Polyphenolics-Rich)
RC401	Vazyme	50 rxn	EUR 283.2

Broad Range DNA Quantification Kit, 100 assays
NS1020-BR-100	Benchmark Scientific	1 each	EUR 131.3

AccuBlue Broad Range dsDNA Quantitation Solution
31009	Biotium	1KIT	EUR 271.2
Description: Minimum order quantity: 1 unit of 1KIT

Easy3-RNA Isolation Kit
FYG501-100P	Yeastern Biotech	100 Preps	Ask for price

Easy3-RNA Isolation Kit
FYG501-500P	Yeastern Biotech	500 Preps	Ask for price

Cartilage RNA Isolation Kit
K2031010	Biochain	1 kit	EUR 502.8
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Easy3-Total NucleicAcid Isolation Kit
FYG504-100ML	Yeastern Biotech	100 ml	Ask for price

Easy3-Total NucleicAcid Isolation Kit
FYG504-500ML	Yeastern Biotech	500 ml	Ask for price

AccuGreenâ„¢ Broad Range dsDNA Quantitation Kit (500 assays)
31069	Biotium	1kit	EUR 267.6
Description: Minimum order quantity: 1 unit of 1kit

AccuGreenâ„¢ Broad Range dsDNA Quantitation Kit (100 assays)
31069-T	Biotium	1kit	EUR 148.8
Description: Minimum order quantity: 1 unit of 1kit

Pharmalyte broad range pH 2.5-5 25mL
17045101	Scientific Laboratory Supplies	EACH	EUR 636.12

Pharmalyte broad range pH 4-6.5 25mL
17045201	Scientific Laboratory Supplies	EACH	EUR 636.12

Pharmalyte broad range pH 5-8 25mL
17045301	Scientific Laboratory Supplies	EACH	EUR 636.12

Pharmalyte broad range pH 8-10.5 25mL
17045501	Scientific Laboratory Supplies	EACH	EUR 636.12

Pharmalyte broad range pH 3-10 25mL
17045601	Scientific Laboratory Supplies	EACH	EUR 530.1

Human Total PSA (t-PSA) ELISA Kit
PRB-5049-TOTAL	Cell Biolabs	96 assays	EUR 686.4

Rapid Blood RNA Isolation Kit
BT4182	Bio Basic	10Preps	EUR 98.36

Rapid Blood RNA Isolation Kit
BT4183	Bio Basic	50Preps	EUR 197.03

Rapid Blood RNA Isolation Kit
BT4184	Bio Basic	250preps	EUR 608.1

Rapid Bacteria RNA Isolation Kit
BS8625	Bio Basic	50Preps	EUR 95.63

Easy3-Plant RNA Isolation Kit
FYG502-100P	Yeastern Biotech	100 Preps	Ask for price

Easy3-Plant RNA Isolation Kit
FYG502-500P	Yeastern Biotech	500 Preps	Ask for price

Rapid Plant RNA Isolation Kit
PT4191	Bio Basic	50Preps	EUR 95.63

EpiMag Viral RNA Isolation Kit
P-9108-050	EpiGentek	50 samples	EUR 152.9

EVery EV RNA Isolation Kit
EVery100B-1	SBI	20 preps	EUR 412

Navy RNA Lysis Kit 50
NAVYE1-RNA	Next Advance	50pack	EUR 237.6
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Navy RNA Lysis Kit 250
NAVYE5-RNA	Next Advance	250pack	EUR 781.2
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Green RNA Lysis Kit 50
GREENE1-RNA	Next Advance	50pack	EUR 216
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Green RNA Lysis Kit 250
GREENE5-RNA	Next Advance	250pack	EUR 685.2
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Pink RNA Lysis Kit 50
PINKE1-RNA	Next Advance	50pack	EUR 213.6
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Pink RNA Lysis Kit 250
PINKE5-RNA	Next Advance	250pack	EUR 673.2
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RNA Lysis Kit 50
REDE1-RNA	Next Advance	50pack	EUR 230.4
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RNA Lysis Kit 250
REDE5-RNA	Next Advance	250pack	EUR 746.4
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RNA Lysis Kit 50
YELLOWE1-RNA	Next Advance	50pack	EUR 230.4
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RNA Lysis Kit 250
YELLOWE5-RNA	Next Advance	250pack	EUR 748.8
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

AccuBlue Broad Range dsDNA Quantitation Solution, trial size
31009-T	Biotium	1KIT	EUR 115.2
Description: Minimum order quantity: 1 unit of 1KIT

AccuGreenâ„¢ Broad Range dsDNA Quantitation Solution (500 assays)
31070	Biotium	1kit	EUR 226.8
Description: Minimum order quantity: 1 unit of 1kit

AccuGreenâ„¢ Broad Range dsDNA Quantitation Solution (100 assays)
31070-T	Biotium	1kit	EUR 130.8
Description: Minimum order quantity: 1 unit of 1kit

Prosi Prestained Protein Marker, Broad Range(11-170KDa)
P8500-040	GenDepot	200ulX2	EUR 271.2

RNA-easy Isolation Reagent
R701-01	Vazyme	100 ml	EUR 201.6

RNA-easy Isolation Reagent
R701-02	Vazyme	200 ml	EUR 254.4

5E Navy RNA Lysis Kit 100
NAVY5E100-RNA	Next Advance	100pack	EUR 543.6
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

5E Navy RNA Lysis Kit 20
NAVY5E20-RNA	Next Advance	20pack	EUR 183.6
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Navy RINO RNA Lysis Kit 50
NAVYR1-RNA	Next Advance	50pack	EUR 242.4
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. RNase free.

Navy RINO RNA Lysis Kit 250
NAVYR5-RNA	Next Advance	250pack	EUR 799.2
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. RNase free.

5E GREEN RNA Lysis Kit 100
GREEN5E100-RNA	Next Advance	100pack	EUR 463.2
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 1000, 5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

5E GREEN RNA Lysis Kit 20
GREEN5E20-RNA	Next Advance	20pack	EUR 165.6
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Green RINO RNA Lysis Kit 50
GREENR1-RNA	Next Advance	50pack	EUR 220.8
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with beads. RNase free.

Green RINO RNA Lysis Kit 250
GREENR5-RNA	Next Advance	250pack	EUR 704.4
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with beads. RNase free.

5E PINK RNA Lysis Kit 100
PINK5E100-RNA	Next Advance	100pack	EUR 456
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

5E PINK RNA Lysis Kit 20
PINK5E20-RNA	Next Advance	20pack	EUR 163.2
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Pink RINO RNA Lysis Kit 50
PINKR1-RNA	Next Advance	50pack	EUR 217.2
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

Pink RINO RNA Lysis Kit 250
PINKR5-RNA	Next Advance	250pack	EUR 691.2
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

5E RED RNA Lysis Kit 100
RED5E100-RNA	Next Advance	100pack	EUR 514.8
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

5E RED RNA Lysis Kit 20
RED5E20-RNA	Next Advance	20pack	EUR 177.6
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RINO RNA Lysis Kit 50
REDR1-RNA	Next Advance	50pack	EUR 234
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

Red RINO RNA Lysis Kit 250
REDR5-RNA	Next Advance	250pack	EUR 765.6
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RINO RNA Lysis Kit 50
YELLOWR1-RNA	Next Advance	50pack	EUR 238.8
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RINO RNA Lysis Kit 250
YELLOWR5-RNA	Next Advance	250pack	EUR 787.2
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

Soil RNA Isolation Mini Kit (50prep)
FASRK-001	Favorgen	50 preps	EUR 180

Soil RNA Isolation Mini Kit (100prep)
FASRK-100	Favorgen	100 preps	EUR 224.4

EpiQuik Magbeads Quick RNA Isolation Kit
P-9106-050	EpiGentek	50 samples	EUR 163.9

EpiQuik Viral RNA Isolation Fast Kit
P-9107-050	EpiGentek	50 samples	EUR 167.2

Human Total PSA (t-PSA) ELISA Kit
PRB-5049-TOTAL-5	Cell Biolabs	5 x 96 assays	EUR 2739.6

AnaPrep Total RNA Extraction Kit
Z1322015	Biochain	1 kit (48 extractions) Including all required plastic disposables	EUR 369.6
Description: This kit is developped for our fully automated magnetic bead-based nucleic acid extraction platform which uses preprogrammed protocols and can process up to 12 samples simultaneously. With the AnaPrep 12 Extractor you will have the option to choose and work with a wide range of sample and reagent volumes. This sturdy, realiable and user-friendly machine will save you both time and expenses while ensuring consistently high quality performance and nucleic acids for your downstream applications.

xTractPure Total RNA extraction Kit
Z2212007	Biochain	1 kit	EUR 499

RNA isolater Total RNA Extraction Reagent
R401-01	Vazyme	100 ml	EUR 159.6

Helixyte™ Green Fluorimetric dsDNA Quantitation Kit *Optimized for Broad Dynamic Range*
17645	AAT Bioquest	200 Tests	EUR 222

Helixyte™ Green Fluorimetric dsDNA Quantitation Kit *Optimized for Broad Dynamic Range*
17646	AAT Bioquest	1000 Tests	EUR 558

Rapid Animal Total RNA Extraction Kit
AT4181	Bio Basic	50Preps	EUR 105.67

Rapid Animal Total RNA Extraction Kit
AT4182	Bio Basic	250preps	EUR 242.7

Plant Total RNA Mini Kit (50prep)
FAPRK-001	Favorgen	50 preps	EUR 198

Plant Total RNA Mini Kit (100prep)
FAPRK-001-1	Favorgen	100 preps	EUR 259.2

Plant Total RNA Mini Kit (300prep)
FAPRK-001-2	Favorgen	300 preps	EUR 484.8

Plant Total RNA Maxi Kit (10prep)
FAPRK-002	Favorgen	10 preps	EUR 241.2

Plant Total RNA Maxi Kit (24prep)
FAPRK-002-1	Favorgen	24 preps	EUR 380.4

Tissue Total RNA Mini Kit (50prep)
FATRK-001	Favorgen	50 preps	EUR 198

Tissue Total RNA Mini Kit (100prep)
FATRK-001-1	Favorgen	100 preps	EUR 259.2

Tissue Total RNA Mini Kit (300prep)
FATRK-001-2	Favorgen	300 preps	EUR 484.8

Tissue Total RNA Maxi Kit (10prep)
FATRK-003	Favorgen	10 preps	EUR 241.2

Tissue Total RNA Maxi Kit (24prep)
FATRK-003-1	Favorgen	24 preps	EUR 380.4

Zenoquick Whole Blood Total RNA Kit
Z5010-050	GenDepot	50 preps	EUR 578.4

Zenoquick Whole Blood Total RNA Kit
Z5010-100	GenDepot	2x50 preps	EUR 967.2

PureXtract RNAsol RNA Isolation Solution, LS
R6103-120	GenDepot	2X60ml	EUR 343.2

AccuBlue Broad Range dsDNA Quantitation Kit with DNA Standard, trial size (200 assays)
31007-T	Biotium	1KIT	EUR 130.8
Description: Minimum order quantity: 1 unit of 1KIT

Total RNA ExtractionMini KitAnimal Tissues
FYG301-50P	Yeastern Biotech	50 Preps	Ask for price

Total RNA ExtractionMini KitAnimal Tissues
FYG302-100P	Yeastern Biotech	100 Preps	Ask for price

Total RNA ExtractionMini KitAnimal Tissues
FYG302-300P	Yeastern Biotech	300 Preps	Ask for price

Total RNA ExtractionMini KitPlant Tissues
FYG303-100P	Yeastern Biotech	100 Preps	Ask for price

Total RNA ExtractionMini KitPlant Tissues
FYG303-300P	Yeastern Biotech	300 Preps	Ask for price

Total RNA ExtractionMini KitPlant Tissues
FYG303-50P	Yeastern Biotech	50 Preps	Ask for price

Total RNA from Lupus: Colon
R1236090Lup-50	Biochain	50 ug	EUR 421.2
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from hypertension: Heart
R1236122Hd-2	Biochain	50 ug	EUR 421.2
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Kidney
R1236142Lup-50	Biochain	50 ug	EUR 421.2
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Liver
R1236149Lup-50	Biochain	50 ug	EUR 421.2
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Lung
R1236152Lup-50	Biochain	50 ug	EUR 421.2
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Pancreas
R1236188Lup-50	Biochain	50 ug	EUR 421.2
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Spleen
R1236246Lup-50	Biochain	50 ug	EUR 421.2
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Stomach
R1236248Lup-50	Biochain	50 ug	EUR 421.2
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Uterus
R1236274Lup-50	Biochain	50 ug	EUR 421.2
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Reagent for Total Exosome Isolation (Culture Media Supplement)
EIR-02	Creative Biolabs	50 ml	EUR 2485.2
Description: This product is used for isolating exosomes from culture media supplement based on chemical precipitation isolation method.

96-well Total RNA Kit (1 plates)
FATRE-96001	Favorgen	1 prep	EUR 232.8

96-well Total RNA Kit (2 plates)
FATRE-96002	Favorgen	2 preps	EUR 337.2

96-well Total RNA Kit (4 plates)
FATRE-96004	Favorgen	4 preps	EUR 537.6

Total RNA Plus Mini Kit (50 prep)
FATRK-P-050	Favorgen	50 preps	EUR 214.8

Total RNA Plus Mini Kit (100 prep)
FATRK-P-100	Favorgen	100 preps	EUR 290.4

AccuBlue Broad Range dsDNA Quantitation Standards, set of nine, 0.5 mL each
31007C	Biotium	1KIT	EUR 164.4
Description: Minimum order quantity: 1 unit of 1KIT

Total RNA ExtractionMini KitBlood/Cultured Cell
FYG301-300P	Yeastern Biotech	300 Preps	Ask for price

Total RNA from Alzheimer's Disease: Brain
R1236035Alz-50	Biochain	50 ug	EUR 421.2
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Pons
R1236071Alz-10	Biochain	10 ug	EUR 421.2
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Diabetic Disease: Colon
R1236090Dia-50	Biochain	50 ug	EUR 421.2
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Colon
R1236090Lcs-50	Biochain	50 ug	EUR 421.2
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Diabetic Disease: Esophagus
R1236106Dia-50	Biochain	50 ug	EUR 421.2
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from hypertension: Interventricular Septum
R1236130Hd-2	Biochain	50 ug	EUR 421.2
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Kidney
R1236142Lcs-50	Biochain	50 ug	EUR 421.2
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Liver
R1236149Lcs-50	Biochain	50 ug	EUR 421.2
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Lung
R1236152Lcs-50	Biochain	50 ug	EUR 421.2
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Diaphragm
R1236169Lcs-50	Biochain	50 ug	EUR 421.2
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Diabetic Disease: Pancreas
R1236188Dia-50	Biochain	50 ug	EUR 421.2
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Pancreas
R1236188Lcs-50	Biochain	50 ug	EUR 421.2
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Rectum
R1236206Lcs-50	Biochain	50 ug	EUR 421.2
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Small Intestine
R1236226Lup-50	Biochain	50 ug	EUR 421.2
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Spleen
R1236246Lcs-50	Biochain	50 ug	EUR 421.2
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Diabetic Disease: Stomach
R1236248Dia-50	Biochain	50 ug	EUR 421.2
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

EZ-10 Spin Column Total RNA Miniprep Kit
BS1361(SK8655)	Bio Basic	50Preps	EUR 112.98

Blood/Cultured Cell Total RNA Mini Kit (50prep)
FABRK-001	Favorgen	50 preps	EUR 198

Blood/Cultured Cell Total RNA Mini Kit (100prep)
FABRK-001-1	Favorgen	100 preps	EUR 259.2

Blood/Cultured Cell Total RNA Mini Kit (300prep)
FABRK-001-2	Favorgen	300 preps	EUR 484.8

Meaning: The current study explores for the first time the changes in proteomes of small extracellular vesicles and urine of patients with vasculitis associated with former kidney relative to healthy controls by LC-MS / MS label. The isolation of vesicles from proteinuric urine samples has been modified to minimize plasma protein contamination and reduce co-insulation of extramuminal proteins. Differentially modified proteins and related routes with a role in AAV pathophysiology have been described and discussed. The results could be useful for the search for potential biomarkers in renal vasculitis associated with ANCa.