Exosome-mediated Hic-5 regulates proliferation and apoptosis of osteosarcoma via Wnt/β-catenin signal pathway

HIC-5 expression was detected in osteosarkoma patients and osteosaroma cell lines by RT-PCR. Then RFP-SH-HIC-5 is transfected into the osteosarcoma cell line. The HIC-5 effect on cell viability, proliferation and apoptosis is assessed by MTT, Edu Kit and Flow Cytometry. Exosom is isolated from mg-63 cell supernatant by an exosomed insulation kit. Exosome-HIC-5 is confirmed by microscope electron transmission, particle size detection and RT-PCR.

Furthermore, the cells of the exosom-hic-5-5 are explored eligibility of cells, proliferation, and apoptosis. Furthermore, the C-IP test is used to identify the relationship between HIC-5 and SMAD4. TCF / LEF and the level of protein components of the WNT / β-Catenin signal are detected by the top luciferase test and Western blot. HIC-5 is regulated in osteosaroma tissue and cells. The decreased expression forced HIC-5 inhibits the proliferation of osteosarkoma cell lines, and induces the apoptosis of MG-63 and HOS. In Vivo, silencing HIC-5 sent the development of tumors. Furthermore, we isolate the exosom of the MG-63 supernatant, the excosom to conclude HIC-5 will regulate the proliferation and level of cell apoptosis of MG-63 and HOS. Furthermore, HIC-5 interacts with SMAD4 and regulates WNT / β-Catenin signals by reducing TCF / LEF activity.

Silencing HIC-5 inhibits proliferation and induces the apoptosis of cell osteosarcoma through deactivating the WNT / β-Catenin signal with the exosome path. Hypoxia is a characteristic of several serious diseases such as cardiovascular disorders and metabolism and cancer. A decrease at the oxygen level of the network induces hypoxic response in cells that seek to adapt to changing conditions. Failure to adapt to prolonged or severe hypoxia can trigger cell death. While some types of cells, such as neurons, are very susceptible to hypoxia, cancer cells utilize the hypoxia environment to undergo tumor growth, angiogenesis and metastasis. Hypoxic-induced processes trigger communication between complex cells and now there is an indication that the extracellular vesicle (EVS) plays a fundamental role in this process. The latest developments in EV isolation and the characterization methodology have raised awareness of the importance of EV purity in functional studies and cargo.

 

Exosomal limitations in the treatment of inflammatory diseases

Background: Despite great interest and many studies, there are currently no integrated standards that describe sequential manipulation with cells to get exosom for clinical use. The use of exosom has become an interesting alternative to cell therapy, because of the flexible properties of these biological vesicles. Enables scientists to manipulate their composition to produce the desired exosom to bring certain drugs, RNA and protein. This study aims to analyze the scientific literature on changes in the functional characteristics of exosom, depending on the manipulation method, which has the potential to contribute to the development of negative effects in the treatment of inflammatory genesis diseases. The choice of insulation method affects the set of protein markers disclosed, nucleic acid and receptors on microparticles.

Various surface receptors present on the exosome membrane can be engineered for the target of the lesion. Exocomals of healthy patients help reduce inflammation, normalize communication between cells and have antifibrotic, antioxidant and cytoprotective effects. Exosom can change the micro environment, but the micro environment can also change the composition of the exosomes.

Conclusion: Ecosomes obtained from sick patients carry markers of the characteristics of the appropriate disease. Such ecosomes can have pro-inflammatory properties, pro-fibrotic, cytotoxic, and oncogenic, and disrupt cellular cooperation. Until now, questions about doses, reactions to recurring giving, and the dose regime has not been fully resolved.

 Exosome-mediated Hic-5 regulates proliferation and apoptosis of osteosarcoma via Wnt/β-catenin signal pathway
Exosome-mediated Hic-5 regulates proliferation and apoptosis of osteosarcoma via Wnt/β-catenin signal pathway

Proteomic exploration based on mass spectrometry of small urinary extracellular vesicles in vasculitis associated with ANCA compared to total urine

The vasculitis associated with the ANCA (AAV) is a rare, but potentially serious self-immune disease, if not nowadays, with increased mortality and morbidity. Finding the first activity biomarkers and the prognosis is very important. The small extracellular vesicles (EVS) isolated from the urine can be considered as a non-invasive source of biomarkers. We evaluated several protocols for the isolation of urinary electric vehicles.

To eliminate contaminating non-vesicular proteins due to AAV associated proteinuria, we used the processing of proteinase K. We have studied the differences in proteomes of small patient events with AAV compared to healthy controls by the freedom of LC-MS / MS labels. In parallel, we performed a similar proteomic analysis of urine samples from identical patients. The results of the study showed significant differences and similarities in the EV proteome and urine, the latter being strongly affected by proteinuria. Using bioinformatic tools, we have explored the modified proteins differently and their related routes by focusing on AAV’s pathophysiology. Our results indicate a significant regulation of Golgi enzymes, such as MAN1A1, which may be involved in the activation of the T cell by N-glycans glycosylation and can play a key role in the pathogenesis and diagnosis of AAV.

Total RNA Isolation Kit

P-9100 50 samples
EUR 347.25
Description: The best epigenetics products

Total RNA Isolation Kit II

FATRS--001 100 preps
EUR 308

Total RNA Isolation Kit II

FATRS--050 50 preps
EUR 216

Total RNA Isolation Kit (Tissue)

P-9102 50 samples
EUR 347.25
Description: reagents widely cited

Total RNA Isolation Kit (Plant)

P-9104 50 samples
EUR 347.25
Description: kits suitable for this type of research

AccuBlue Broad Range RNA Quantitation Kit (1000 assays)

31073 1kit
EUR 363
Description: Minimum order quantity: 1 unit of 1kit

AccuBlue Broad Range RNA Quantitation Kit (200 assays)

31073-T 1kit
EUR 141
Description: Minimum order quantity: 1 unit of 1kit

FastPure Cell/Tissue Total RNA Isolation Kit

RC101 50 rxn
EUR 229

AccuBlue Broad Range dsDNA Quantitation Kit

31007 1KIT
EUR 312
Description: Minimum order quantity: 1 unit of 1KIT

FastPure Plant Total RNA Isolation Kit (Polysaccharides/Polyphenolics-Rich)

RC401 50 rxn
EUR 236

AccuBlue Broad Range dsDNA Quantitation Solution

31009 1KIT
EUR 226
Description: Minimum order quantity: 1 unit of 1KIT

AccuGreenâ„¢ Broad Range dsDNA Quantitation Kit (500 assays)

31069 1kit
EUR 223
Description: Minimum order quantity: 1 unit of 1kit

AccuGreenâ„¢ Broad Range dsDNA Quantitation Kit (100 assays)

31069-T 1kit
EUR 124
Description: Minimum order quantity: 1 unit of 1kit

Easy3-Total NucleicAcid Isolation Kit

FYG504-100ML 100 ml Ask for price

Easy3-Total NucleicAcid Isolation Kit

FYG504-500ML 500 ml Ask for price

Easy3-RNA Isolation Kit

FYG501-100P 100 Preps Ask for price

Easy3-RNA Isolation Kit

FYG501-500P 500 Preps Ask for price

Cartilage RNA Isolation Kit

K2031010 1 kit
EUR 419
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Easy3-Plant RNA Isolation Kit

FYG502-100P 100 Preps Ask for price

Easy3-Plant RNA Isolation Kit

FYG502-500P 500 Preps Ask for price

Rapid Bacteria RNA Isolation Kit

BS8625 50Preps
EUR 79.69
  • Product category: Molecular Biology Kits/RNA - Extraction/Bacteria

Rapid Blood RNA Isolation Kit

BT4182 10Preps
EUR 81.97
  • Product category: Molecular Biology Kits/RNA - Extraction/Blood

Rapid Blood RNA Isolation Kit

BT4183 50Preps
EUR 164.19
  • Product category: Molecular Biology Kits/RNA - Extraction/Blood

Rapid Blood RNA Isolation Kit

BT4184 250preps
EUR 506.75
  • Product category: Molecular Biology Kits/RNA - Extraction/Blood

Rapid Plant RNA Isolation Kit

PT4191 50Preps
EUR 79.69
  • Product category: Molecular Biology Kits/RNA - Extraction/Plant

AccuBlue Broad Range dsDNA Quantitation Solution, trial size

31009-T 1KIT
EUR 96
Description: Minimum order quantity: 1 unit of 1KIT

AccuGreenâ„¢ Broad Range dsDNA Quantitation Solution (500 assays)

31070 1kit
EUR 189
Description: Minimum order quantity: 1 unit of 1kit

AccuGreenâ„¢ Broad Range dsDNA Quantitation Solution (100 assays)

31070-T 1kit
EUR 109
Description: Minimum order quantity: 1 unit of 1kit

Prosi Prestained Protein Marker, Broad Range(11-170KDa)

P8500-040 200ulX2
EUR 226

Human Total PSA (t-PSA) ELISA Kit

PRB-5049-TOTAL 96 assays
EUR 572

RNA-easy Isolation Reagent

R701-01 100 ml
EUR 168

RNA-easy Isolation Reagent

R701-02 200 ml
EUR 212

Green RNA Lysis Kit 50

GREENE1-RNA 50pack
EUR 180
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Green RNA Lysis Kit 250

GREENE5-RNA 250pack
EUR 571
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Navy RNA Lysis Kit 50

NAVYE1-RNA 50pack
EUR 198
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Navy RNA Lysis Kit 250

NAVYE5-RNA 250pack
EUR 651
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Pink RNA Lysis Kit 50

PINKE1-RNA 50pack
EUR 178
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Pink RNA Lysis Kit 250

PINKE5-RNA 250pack
EUR 561
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RNA Lysis Kit 50

REDE1-RNA 50pack
EUR 192
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RNA Lysis Kit 250

REDE5-RNA 250pack
EUR 622
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RNA Lysis Kit 50

YELLOWE1-RNA 50pack
EUR 192
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RNA Lysis Kit 250

YELLOWE5-RNA 250pack
EUR 624
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Total RNA Extraction Kit

K2014005 1 kit
EUR 344
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

5E GREEN RNA Lysis Kit 100

GREEN5E100-RNA 100pack
EUR 386
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 1000, 5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

5E GREEN RNA Lysis Kit 20

GREEN5E20-RNA 20pack
EUR 138
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Green RINO RNA Lysis Kit 50

GREENR1-RNA 50pack
EUR 184
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with beads. RNase free.

Green RINO RNA Lysis Kit 250

GREENR5-RNA 250pack
EUR 587
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with beads. RNase free.

5E Navy RNA Lysis Kit 100

NAVY5E100-RNA 100pack
EUR 453
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

5E Navy RNA Lysis Kit 20

NAVY5E20-RNA 20pack
EUR 153
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Navy RINO RNA Lysis Kit 50

NAVYR1-RNA 50pack
EUR 202
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. RNase free.

Navy RINO RNA Lysis Kit 250

NAVYR5-RNA 250pack
EUR 666
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. RNase free.

5E PINK RNA Lysis Kit 100

PINK5E100-RNA 100pack
EUR 380
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

5E PINK RNA Lysis Kit 20

PINK5E20-RNA 20pack
EUR 136
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Pink RINO RNA Lysis Kit 50

PINKR1-RNA 50pack
EUR 181
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

Pink RINO RNA Lysis Kit 250

PINKR5-RNA 250pack
EUR 576
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

5E RED RNA Lysis Kit 100

RED5E100-RNA 100pack
EUR 429
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

5E RED RNA Lysis Kit 20

RED5E20-RNA 20pack
EUR 148
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RINO RNA Lysis Kit 50

REDR1-RNA 50pack
EUR 195
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

Red RINO RNA Lysis Kit 250

REDR5-RNA 250pack
EUR 638
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RINO RNA Lysis Kit 50

YELLOWR1-RNA 50pack
EUR 199
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RINO RNA Lysis Kit 250

YELLOWR5-RNA 250pack
EUR 656
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

Soil RNA Isolation Mini Kit (50prep)

FASRK-001 50 preps
EUR 150

Soil RNA Isolation Mini Kit (100prep)

FASRK-100 100 preps
EUR 187

Human Total PSA (t-PSA) ELISA Kit

PRB-5049-TOTAL-5 5 x 96 assays
EUR 2283

AnaPrep Total RNA Extraction Kit

Z1322015 1 kit (48 extractions) Including all required plastic disposables
EUR 308
Description: This kit is developped for our fully automated magnetic bead-based nucleic acid extraction platform which uses preprogrammed protocols and can process up to 12 samples simultaneously. With the AnaPrep 12 Extractor you will have the option to choose and work with a wide range of sample and reagent volumes. This sturdy, realiable and user-friendly machine will save you both time and expenses while ensuring consistently high quality performance and nucleic acids for your downstream applications.

RNA isolater Total RNA Extraction Reagent

R401-01 100 ml
EUR 133

PureXtract RNAsol RNA Isolation Solution, LS

R6103-120 2X60ml
EUR 286

Plant Total RNA Mini Kit (50prep)

FAPRK-001 50 preps
EUR 165

Plant Total RNA Mini Kit (100prep)

FAPRK-001-1 100 preps
EUR 216

Plant Total RNA Mini Kit (300prep)

FAPRK-001-2 300 preps
EUR 404

Plant Total RNA Maxi Kit (10prep)

FAPRK-002 10 preps
EUR 201

Plant Total RNA Maxi Kit (24prep)

FAPRK-002-1 24 preps
EUR 317

Tissue Total RNA Mini Kit (50prep)

FATRK-001 50 preps
EUR 165

Tissue Total RNA Mini Kit (100prep)

FATRK-001-1 100 preps
EUR 216

Tissue Total RNA Mini Kit (300prep)

FATRK-001-2 300 preps
EUR 404

Tissue Total RNA Maxi Kit (10prep)

FATRK-003 10 preps
EUR 201

Tissue Total RNA Maxi Kit (24prep)

FATRK-003-1 24 preps
EUR 317

Zenoquick Whole Blood Total RNA Kit

Z5010-050 50 preps
EUR 482

Zenoquick Whole Blood Total RNA Kit

Z5010-100 2x50 preps
EUR 806

Rapid Animal Total RNA Extraction Kit

AT4181 50Preps
EUR 88.06
  • Product category: Molecular Biology Kits/RNA - Extraction/Animal

Rapid Animal Total RNA Extraction Kit

AT4182 250preps
EUR 202.25
  • Product category: Molecular Biology Kits/RNA - Extraction/Animal

AccuBlue Broad Range dsDNA Quantitation Kit with DNA Standard, trial size (200 assays)

31007-T 1KIT
EUR 109
Description: Minimum order quantity: 1 unit of 1KIT

Reagent for Total Exosome Isolation (Culture Media Supplement)

EIR-02 50 ml
EUR 2071
Description: This product is used for isolating exosomes from culture media supplement based on chemical precipitation isolation method.

AccuBlue Broad Range dsDNA Quantitation Standards, set of nine, 0.5 mL each

31007C 1KIT
EUR 137
Description: Minimum order quantity: 1 unit of 1KIT

Total RNA ExtractionMini KitAnimal Tissues

FYG301-50P 50 Preps Ask for price

Total RNA ExtractionMini KitAnimal Tissues

FYG302-100P 100 Preps Ask for price

Total RNA ExtractionMini KitAnimal Tissues

FYG302-300P 300 Preps Ask for price

Total RNA ExtractionMini KitPlant Tissues

FYG303-100P 100 Preps Ask for price

Total RNA ExtractionMini KitPlant Tissues

FYG303-300P 300 Preps Ask for price

Total RNA ExtractionMini KitPlant Tissues

FYG303-50P 50 Preps Ask for price

Total RNA from Lupus: Colon

R1236090Lup-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from hypertension: Heart

R1236122Hd-2 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Kidney

R1236142Lup-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Liver

R1236149Lup-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Lung

R1236152Lup-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Pancreas

R1236188Lup-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Spleen

R1236246Lup-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Stomach

R1236248Lup-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Uterus

R1236274Lup-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

96-well Total RNA Kit (1 plates)

FATRE-96001 1 prep
EUR 194

96-well Total RNA Kit (2 plates)

FATRE-96002 2 preps
EUR 281

96-well Total RNA Kit (4 plates)

FATRE-96004 4 preps
EUR 448

Total RNA Plus Mini Kit (50 prep)

FATRK-P-050 50 preps
EUR 179

Total RNA Plus Mini Kit (100 prep)

FATRK-P-100 100 preps
EUR 242

Exosome Isolation kit (for serum & plasma -Exosome-RNA-NGS)

P101NGS NULL
EUR 0

Total RNA ExtractionMini KitBlood/Cultured Cell

FYG301-300P 300 Preps Ask for price

Total RNA from Alzheimer's Disease: Brain

R1236035Alz-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Pons

R1236071Alz-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Diabetic Disease: Colon

R1236090Dia-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Colon

R1236090Lcs-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Diabetic Disease: Esophagus

R1236106Dia-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from hypertension: Interventricular Septum

R1236130Hd-2 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Kidney

R1236142Lcs-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Liver

R1236149Lcs-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Lung

R1236152Lcs-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Diaphragm

R1236169Lcs-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Diabetic Disease: Pancreas

R1236188Dia-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Pancreas

R1236188Lcs-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Rectum

R1236206Lcs-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Small Intestine

R1236226Lup-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Spleen

R1236246Lcs-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Diabetic Disease: Stomach

R1236248Dia-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Blood/Cultured Cell Total RNA Mini Kit (50prep)

FABRK-001 50 preps
EUR 165

Blood/Cultured Cell Total RNA Mini Kit (100prep)

FABRK-001-1 100 preps
EUR 216

Blood/Cultured Cell Total RNA Mini Kit (300prep)

FABRK-001-2 300 preps
EUR 404

Blood/Cultured Cell Total RNA Maxi Kit (10prep)

FABRK-003 10 preps
EUR 201

Blood/Cultured Cell Total RNA Maxi Kit (24prep)

FABRK-003-1 24 preps
EUR 317

AnaPrep Total RNA Extraction Kit with DNase-Treatment

Z1322017 1 kit (48 extractions) Including all required plastic disposables
EUR 405
Description: This kit is developped for our fully automated magnetic bead-based nucleic acid extraction platform which uses preprogrammed protocols and can process up to 12 samples simultaneously. With the AnaPrep 12 Extractor you will have the option to choose and work with a wide range of sample and reagent volumes. This sturdy, realiable and user-friendly machine will save you both time and expenses while ensuring consistently high quality performance and nucleic acids for your downstream applications.

EZ-10 Spin Column Total RNA Miniprep Kit

BS1361(SK8655) 50Preps
EUR 94.15
  • Product category: Molecular Biology Kits/RNA - Extraction/Animal

Mitochondria Isolation Kit

AR0156 1 kit
EUR 106

miRNA Isolation Kit

FAMIK--001 Rxns: 100
EUR 332
Description: Contents of the kit:
  • Rxns:100Lysis
  • Buffer-10 ml
  • Acid-Phenol-10 ml
  • RNA Column and Collection Tube-100 sets
  • Wash Buffer-10 ml
  • Release Buffer-0.5 ml

miRNA Isolation Kit

FAMIK--002 Rxns: 50
EUR 223

miRNA Isolation Kit

FYG304-100P 100 Preps Ask for price

MicroRNA Isolation Kit

KS341025 1 kit
EUR 256
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Preadipocyte Isolation Kit

K583-5
EUR 533

Microsome Isolation Kit

K249-50
EUR 528

Endosome Isolation Kit

P528 NULL
EUR 0

ExoQuick Exosome Isolation and RNA Purification kit (for Serum and Plasma)

EQ806A-1 20 preps
EUR 554

Meaning: The current study explores for the first time the changes in proteomes of small extracellular vesicles and urine of patients with vasculitis associated with former kidney relative to healthy controls by LC-MS / MS label. The isolation of vesicles from proteinuric urine samples has been modified to minimize plasma protein contamination and reduce co-insulation of extramuminal proteins. Differentially modified proteins and related routes with a role in AAV pathophysiology have been described and discussed. The results could be useful for the search for potential biomarkers in renal vasculitis associated with ANCa.